Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	49.4%	96.7 / 35.8	77	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (8/31);  new base G (38/0);  total (46/31)
Fisher's exact test for biased strand distribution p-value = 2.31e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434736‑2435030                                                                                                                                                   |                                                                                                                                                      gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATc                                                                                                                                                   <  2:1254031/151‑1 (MQ=255)   ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATcc                                                                                                                                                  <  1:867907/150‑1 (MQ=255)    cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCCAACCGGAGAGGGGAATACa                                                                                                                                                 >  2:135813/1‑150 (MQ=255)    cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAc                                                                                                                                                <  1:453026/151‑1 (MQ=255)    cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAc                                                                                                                                                <  1:210109/151‑1 (MQ=255)       atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAaccacc                                                                                                                                               >  2:135540/1‑149 (MQ=255)         ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgcg                                                                                                                                            <  1:42765/150‑1 (MQ=255)               aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGTCAGGGGAAACCACCGCGGCCAGa                                                                                                                                      >  1:925903/1‑150 (MQ=255)                tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGCTTTTGGAGAACATACCGGGGGGGGTAATACACCCCCAcc                                                                                                                                         >  2:1143748/1‑146 (MQ=255)                  ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATCCACCCCGACCAGATa                                                                                                                                    >  1:886905/1‑149 (MQ=255)                  ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa                                                                                                                                  <  2:676524/151‑1 (MQ=255)                  ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa                                                                                                                                  <  2:882602/151‑1 (MQ=255)                  ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACa                                                                                                                                  <  2:246578/151‑1 (MQ=255)                  ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGATCAAACCGGGCAGGGGAAACCACCCCGCCCCGATa                                                                                                                                    >  2:786166/1‑149 (MQ=255)                  ccTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGAAAATCCTACCCGGCAGGGGAAaccacccccaac                                                                                                                                         >  2:581900/1‑138 (MQ=255)                    ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCCCGGCCAGATa                                                                                                                                    >  1:957801/1‑147 (MQ=255)                     tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAG‑cc                                                                                                                              >  1:311182/1‑151 (MQ=255)                          tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATAACGGGCAGGGGAATTCACCCCGCCCCGAAAAAG‑CCCTTcc                                                                                                                         >  2:502315/1‑151 (MQ=255)                              cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAATATAACGGTCAGGGGAATCCACCCCGACCAGATACAA‑CCCTTCCCCtt                                                                                                                     >  2:336369/1‑151 (MQ=255)                              cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGGCCCGATAAAA‑CCCCTCCGCtt                                                                                                                     >  1:684953/1‑151 (MQ=255)                                   ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCCGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCCCtttt                                                                                                                   >  2:437647/1‑148 (MQ=255)                                   ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCCGGGGAATCCACCCCGACCAGATACAGCCCCTTCCCCtttt                                                                                                                   >  2:670393/1‑149 (MQ=255)                                   ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATAATAACGGGCAGGGGAAACAACCCAGGCCAGAAACAA‑CCCTTccccct                                                                                                                     >  1:1261093/1‑142 (MQ=255)                                     cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCg                                                                                                               <  1:117233/150‑1 (MQ=255)                                      aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATTAAACCCGACAGGGGAATCTACCGCCAACCGATATAA‑CCCCTCCGCTTTTTccct                                                                                                              >  2:607474/1‑148 (MQ=255)                                        gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTtgc                                                                                                           <  2:1038198/151‑1 (MQ=255)                                           gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTtgct                                                                                                          <  1:162350/149‑1 (MQ=255)                                             aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCCCGCCCAGAAACAG‑CCCTTCCCCTTTTTCCCTTTCTgccc                                                                                                      >  1:422482/1‑150 (MQ=255)                                              aCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGGCAGGGGAAACCACCGCGACCAGATACAG‑CCCCTCCGCTTTTTCCCTTGCTgccgc                                                                                                     >  1:469806/1‑151 (MQ=255)                                                tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAAACCACCGCGACCCGATACAG‑CCCTTCCCCTTTTTCCGGTGCTgccgcc                                                                                                    >  2:1147780/1‑150 (MQ=255)                                                    ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAATTCACCCCGACCAGATACAG‑ACCTTCCCCTTTTTCCGTTGCTtccccc                                                                                                    >  2:48357/1‑146 (MQ=255)                                                     tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGTCAGGGGAAATCACCCCGACCAGATAAAG‑CCCTTCCCCTTTTGCCGCTGCTGCCGCCCGCGt                                                                                               >  2:1073298/1‑150 (MQ=255)                                                       gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCCGGCAGGGGAATCCCCCGCGGCCCGATACAG‑CCCCTCCGCCTTTGCCGGTGATGCCGCCCGCGGTCt                                                                                            >  1:595633/1‑151 (MQ=255)                                                            aGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACAG‑CCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTcc                                                                                        >  1:669583/1‑150 (MQ=255)                                                             gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCtt                                                                                      <  1:502319/151‑1 (MQ=255)                                                              ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCttt                                                                                     <  1:177593/151‑1 (MQ=255)                                                              ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCttt                                                                                     <  2:1067250/151‑1 (MQ=255)                                                                  aaaTGTTCCGCGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCCTTCCGCCCAGGGGAATACCACGCGACCCACCACAG‑CCCTTTCCCTCTTTCCGGTCGTTCCGCCAGCGGTCTGGCttttttc                                                                                  >  2:780480/1‑147 (MQ=255)                                                                   aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGATGTTATACCCGTCAGGGGAATCCATCGCGGGCCGAAACAG‑CCCCTCCTCTTTTTCCGGTGGTGCCCCCAGCGGTCTGTCCttttgcgc                                                                                >  2:1210324/1‑146 (MQ=255)                                                                       ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGGCAGGGGAATCCACCCCGACCAGATACAG‑CCCTTCCCCTTTTTCCGTTGCTGCCCCCAGCGTTCTTTCCTTTtccgcc                                                                               >  2:980184/1‑148 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCaa                                                                          <  1:1196898/151‑1 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                           <  1:982053/150‑1 (MQ=255)                                                                         ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                           <  1:1176593/150‑1 (MQ=255)                                                                            aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGCCCAGATAAAG‑GCCTTCCGCTTTTTCCCGTTCTGCCGCCCGCGTTCTGTCCTTTTCCGCCCGCAActc                                                                       >  2:679122/1‑151 (MQ=255)                                                                            aGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActc                                                                       <  2:217036/151‑1 (MQ=255)                                                                              cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAAACCACCGCGACCAGATACAA‑CCCCTCCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCGCCAGCAActc                                                                       >  1:330638/1‑149 (MQ=255)                                                                               attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACCGGCAGGGGAATCCACCGCGACCCGATACAG‑CCCTTTCGCTTTTTCCGTTGCTGCCCCCCGCGGTCTGTCCTTTTCCGCCCGCCAccc                                                                       >  2:856350/1‑146 (MQ=255)                                                                                 tattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCt                                                                   <  2:941989/150‑1 (MQ=255)                                                                                  attCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATGATACCGGTCAGGGGAATCCACCGCGGCCAGATACAA‑CCCCTCCGCCTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTGCCGCCCGCAACTCTTCTa                                                                  >  2:596348/1‑150 (MQ=255)                                                                                    tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATc                                                                <  1:24549/150‑1 (MQ=255)                                                                                       cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAA‑CCCTTCCGCTTTTTCCGTTGCTGCCGCCCGCGGTCTGCCCTTTTCCGCCCGCAACCCTGCTATCCCGc                                                            >  2:191647/1‑151 (MQ=255)                                                                                           aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct                                                         <  2:28209/150‑1 (MQ=255)                                                                                           aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCtctc                                                        <  1:38282/151‑1 (MQ=255)                                                                                             tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCAAACCCGTCAGGGGAATCCACCGCGGCCCCATACAG‑CCCTTTCGCCTTTTCCGTTGCTGCCGCCAGGGTTTTGTCCTTTTTCGCCCGCAACGCTGCTCTCCAGCTCTccc                                                      >  1:926675/1‑150 (MQ=255)                                                                                               cGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCCTCCGCTTTTGCCGTTGTTGCCGCCCGCGTTCTGTCCTTTTCCGCCCGCAACTCTTCTATCCAGCTCTcccg                                                     >  1:325162/1‑148 (MQ=255)                                                                                               cGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCCGTCAGGGGAATCCACCCCGACCAGATACAA‑CCCTTCCCCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCTTTTTCGCCCGCAACTCTGCTATTCAGCTCTcccg                                                     >  1:812272/1‑148 (MQ=255)                                                                                               cGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGc                                                    <  1:733702/151‑1 (MQ=255)                                                                                                gCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGTCAGGGGAATCCACCCCGACCCGATAAAG‑CCCTTCCCCTTTTTCCGGTGCTGCCCCCCGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTATCCAGCTCTCCGGc                                                    >  1:1256595/1‑150 (MQ=255)                                                                                                gCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCt                                                   <  1:123001/151‑1 (MQ=255)                                                                                                  cAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCCGGCAGGGGAAACCACCCCGACCAGATAAAG‑CCCCTCCGCCTTTTCCGCTGCTGCCCCCAGCGGTCTGTCCTTTTCCCCCCGCAAATCTGCTATCCAGCTCTcccg                                                     >  2:194160/1‑145 (MQ=255)                                                                                                     aaaTAGCGGGCCCATTGGCGGGTTTGGAAAATCATACCCGGCAAGGGAAATCACCCCCACCAGATACAG‑CCCTTCCCCTTTTGCCCTTGCTGCCCCCCGCGGTCTGTCCCTTTCCGCCCGCAACACTGCTATTCAGCTCTCCGGCTgg                                                 >  2:285034/1‑148 (MQ=255)                                                                                                         aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                           <  2:330635/150‑1 (MQ=255)                                                                                                          gCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCCGGGGAATCCACCCCGACCAGATACAG‑CCCTTCCGCTTTTTCCGGTGGTGCCCCCCGCGGTCTTTCCTTTTCCGCCCGCAACTCTGCTATCCAGCTCTCCCGCTGGTTGTggg                                          >  2:806993/1‑150 (MQ=255)                                                                                                            gggCCCATTGGCGGTTTTTGGAGATCATAACGGGCAGGGGAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa                                        >  2:629311/1‑150 (MQ=255)                                                                                                                ccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGAc                                   <  2:44661/151‑1 (MQ=255)                                                                                                                       cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                            >  1:170616/1‑151 (MQ=255)                                                                                                                        ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                           <  2:929849/151‑1 (MQ=255)                                                                                                                           tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg                         >  1:890742/1‑150 (MQ=255)                                                                                                                             tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                      >  1:1192458/1‑151 (MQ=255)                                                                                                                              ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                      <  1:50095/150‑1 (MQ=255)                                                                                                                               gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                    >  1:149430/1‑151 (MQ=255)                                                                                                                                       tACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAatat            >  2:533368/1‑151 (MQ=255)                                                                                                                                        aCCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAatat            <  2:269208/150‑1 (MQ=255)                                                                                                                                          cGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc         >  2:872097/1‑151 (MQ=255)                                                                                                                                          cGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTcc         <  1:732248/151‑1 (MQ=255)                                                                                                                                           ggTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt        >  1:205393/1‑151 (MQ=255)                                                                                                                                                 ggTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat  <  2:1070191/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAG‑CCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT  >  NC_000913/2434736‑2435030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑