Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,0550AC45.2% 121.1 / 14.1 75V455G (GTG→GGG) tdcEpyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase
Reads supporting (aligned to +/- strand):  ref base A (33/7);  new base C (0/33);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 3.02e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTT  >  NC_000913/3260906‑3261205
                                                                                                                                                      |                                                                                                                                                      
tGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAc                                                                                                                                                       >  2:681537/1‑151 (MQ=255)
  tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTcccccc                                                                                                                                                     >  1:425940/1‑151 (MQ=255)
    gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCAcccc                                                                                                                                                    <  2:1211308/150‑1 (MQ=255)
     tGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCccgc                                                                                                                                                  >  1:463782/1‑151 (MQ=255)
        aTGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGt                                                                                                                                               >  2:491358/1‑151 (MQ=255)
         tGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGtt                                                                                                                                              >  2:507543/1‑151 (MQ=255)
          gATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGtt                                                                                                                                              >  2:877648/1‑150 (MQ=255)
           atatTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTa                                                                                                                                             >  2:1270278/1‑150 (MQ=255)
             atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                                                                                          >  1:810806/1‑151 (MQ=255)
             atTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAAtt                                                                                                                                          >  1:681188/1‑151 (MQ=255)
                        tgatttccgcccccGCCACCCAGTCAATGAAGTGATCGAGGCTGTCCTTCTCTTGGTCTTAATCCAGCCCTTCGTCCATCAGGGGTGCTTTTTGCGGCCCGACGTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGagc                                                                                                                                 <  2:932544/137‑1 (MQ=255)
                        tGATTTACTGCCCCGCCAGCCTGCCCATGAAGTGATCGAGGCTGTCCATCCCTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGCTTTTTTCGGCCCGCCCTGAATCTTCAGTTTCTCGTCCCCCCCGCCGTTAATTGCGTAGagc                                                                                                                                 <  2:172848/149‑1 (MQ=255)
                        tGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagc                                                                                                                                 >  1:876417/1‑149 (MQ=255)
                         gATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGagcagc                                                                                                                              >  1:700619/1‑151 (MQ=255)
                           tttcgcgCACCCCCAGCAAGTCCATGAAGTGGCCGGGGCTGTCCATCACTTTGTCGTAATCCAGCACGTGGTCCATCGGCGGTGGTTTTTTCGCCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTaga                                                                                                                                   <  2:610183/138‑1 (MQ=255)
                           tGTCCTGCCCCGCCAGCCATTCCATGAGGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCCCGTCGCCCACCAGGGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCg                                                                                                                             <  2:482278/150‑1 (MQ=255)
                             tACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGttt                                                                                                                          >  1:825976/1‑151 (MQ=255)
                                tGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCg                                                                                                                       >  2:457286/1‑151 (MQ=255)
                                 gCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCACGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGc                                                                                                                      >  2:536861/1‑151 (MQ=255)
                                  cccccccagccagTCCATGAAGTGATCGAGGCTTTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGGGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCg                                                                                                                       <  1:677090/144‑1 (MQ=255)
                                    cccccagccagTCCATGAAGTGATCGAGGCTGTCCATCCCTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                                                                                   <  1:216433/148‑1 (MQ=39)
                                    cccccacccagCCCATGAAGTGATCGGGGCTGTCCATCACTTTGTCTAACCCCACCCCGTCTCCCATCGGCGGTGTTTTTTTCGGCCCGCCCGAAATCTTCGCCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCa                                                                                                                    <  2:778310/147‑1 (MQ=255)
                                    ccGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCATTTTGTCGTAATCCAGCCCGTCGTCCATCAGCGGTGTTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCa                                                                                                                    <  1:912418/150‑1 (MQ=255)
                                    ccGCCACCAAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCCCGTCTTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAg                                                                                                                   <  1:1101333/151‑1 (MQ=255)
                                       ccagccagTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGtt                                                                                                                >  2:155391/1‑151 (MQ=255)
                                           ccagTCAAGGAAGGGATCGGGGCTGCCCATCACTTTGTCGAAATCCAGCACGCCGCCCACCAGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTGGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                               <  2:388839/148‑1 (MQ=255)
                                              gTCCATGAAGTGATCGAGGCTGTCCATCATTTTGTCGAAACCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTAAATCTTCAGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAgcg                                                                                                            <  2:501514/148‑1 (MQ=255)
                                               tCCATGAAGAGTCCGAGGCTGTCCATCATTTTGCCGAAACCCAGCGCGTCGCCCATCAGCGGCGTTTTTTTCGGCCCCCCCTGAATCTTCAGCTTCTCCTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTa                                                                                                               <  1:547471/144‑1 (MQ=255)
                                                       gTGATCGAGGCTGTCCATCTCTTTGTCGTAATCCAGCCCGTCGTCCATCAGCGGTGTTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAg                                                                                                <  1:1204117/151‑1 (MQ=255)
                                                       gTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCaa                                                                                                  >  1:252811/1‑149 (MQ=255)
                                                             ggggCTGTCCATCTTTTTGTCGTAACCCAGCCCGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGCCCTGAATTTCCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACt                                                                                            <  2:589136/147‑1 (MQ=255)
                                                             gAGGTTGCCCATCCCTTTGTCGTAACCCAGCACGTCGTCCATCGGCGGTGTTGTTTTCGGCCCGACCGGACCCTCCAGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGa                                                                                               <  2:722474/146‑1 (MQ=255)
                                                             gAGGCTGTCCATCACTTTGCCGTAACCCGGCCCGCCGTCCATCAGCGTTCCTGTTTTCGCCCAGCCCTAAATTTTCGGCTTCCCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACt                                                                                            <  2:223798/149‑1 (MQ=255)
                                                              aGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTg                                                                                           >  2:74851/1‑149 (MQ=255)
                                                                   gcccATCAGTTTGTCGTAGACCAGGTGATCGACCGTCAGGGTTGGTGTTGGGGGCCCGCCCTAATACTGCAGGTTCTCCCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTg                                                                                     <  1:154732/148‑1 (MQ=255)
                                                                          aCTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                                                                              >  1:132357/1‑150 (MQ=255)
                                                                          aCTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCa                                                                              >  1:987915/1‑150 (MQ=255)
                                                                           cTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAAt                                                                            >  1:1254535/1‑151 (MQ=255)
                                                                            tttGTCGTAACCCAGCACGCCGTCCATGAGCGGGTCTTTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTAcc                                                                               <  2:335693/147‑1 (MQ=255)
                                                                             ttGTCGTAATCCAGCCCGTCGTCCATCAGCGGATCTGTTTTCGCCCCGACCGGAATCTTCAGCTTCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCaa                                                                             <  1:900746/148‑1 (MQ=255)
                                                                              tGTCGTAATCCAGCCCTCCGTCCATGGGCGGTGCTGTTTCCGGCCCGACCGGAATTTTCGGCTTCTCTTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCa                                                                          <  1:1062942/150‑1 (MQ=255)
                                                                               gTCGTAACCCAGCGCGTCGTCCATCGGCGGTGTTTTTTCCGCCCCGACCTGATTTTCCAGCTTCTGGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCa                                                                          <  2:964091/149‑1 (MQ=255)
                                                                                   tAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTg                                                                    >  2:148802/1‑151 (MQ=255)
                                                                                    aaTCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTgg                                                                   >  1:633683/1‑151 (MQ=255)
                                                                                    aaTCCAGCACGTCGCCCACCAGCGGTGCTTTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCAtt                                                                     <  1:902347/149‑1 (MQ=255)
                                                                                       ccAGCCCGTCCTCCATCGGCGGTGCTGTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCt                                                                <  2:421332/151‑1 (MQ=255)
                                                                                           cACGTCGTCCATCGGCGGTGCTTTTTTCGGCCCGACCGGAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACg                                                            <  2:1162778/151‑1 (MQ=255)
                                                                                           cACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACg                                                            >  1:164336/1‑151 (MQ=255)
                                                                                              gtcgtcCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgc                                                           >  2:58398/1‑149 (MQ=255)
                                                                                              gtcgtcCATCAGCGGCGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgc                                                           >  2:1259989/1‑149 (MQ=255)
                                                                                                   ccttcggCGGTGTTTTTTTCGGCCCGCCCTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGcagcag                                                      <  2:1154078/143‑1 (MQ=255)
                                                                                                   ccaccaGCGGTGCTGTTTTCGGCCCGCCTTAAATCTTCACCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAgg                                                     <  2:1046882/150‑1 (MQ=255)
                                                                                                     aTCAGGGGGGCTGTTTTCGGCCCCACCGAAACTTCCCGCTTTTCCACCGCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACgcagca                                                       <  2:303112/146‑1 (MQ=255)
                                                                                                       cAGCGGTGCTTTTTGCGCCCCGACCTGAATTTTCAGTTTCCGGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGc                                                    <  2:245583/147‑1 (MQ=255)
                                                                                                        aGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcg                                               >  1:886529/1‑151 (MQ=255)
                                                                                                         gCGGTGTTTTTTCCGGCCCGCCCTAAATCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAAt                                                 <  1:385675/148‑1 (MQ=255)
                                                                                                          cGGGGCGGTTTTCGCCCCAACCTCATTCTTCTGCTTCTCGCCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAAt                                                 <  2:1120246/147‑1 (MQ=255)
                                                                                                           ggTGCTGTTTTCGGCCCGCCCTAACTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATcgcg                                             <  2:878305/150‑1 (MQ=255)
                                                                                                               cTGTCTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtc                                        >  2:1259877/1‑151 (MQ=255)
                                                                                                                    ttCGGCCCGACCTGATTCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAAtcgtcg                                    <  2:843262/150‑1 (MQ=255)
                                                                                                                      cGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTg                                 >  2:281798/1‑151 (MQ=255)
                                                                                                                       ggCCCGACCTGATTCTTCAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCt                                  <  2:700610/149‑1 (MQ=255)
                                                                                                                        gCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGtt                               >  2:31235/1‑151 (MQ=255)
                                                                                                                          ccGCCCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGa                             <  2:1220904/151‑1 (MQ=255)
                                                                                                                          ccGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCACTCGCGTAATCGTCGCTGTTg                              >  2:223699/1‑150 (MQ=255)
                                                                                                                            gccttGAGTCTTCTCCTCCTCCCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGaa                            <  2:1234520/146‑1 (MQ=255)
                                                                                                                            gACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGa                             >  2:825918/1‑149 (MQ=255)
                                                                                                                              ccTGAATCTTCAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGaa                            <  2:1168583/148‑1 (MQ=255)
                                                                                                                                         cAGCTTCTCGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACg                   <  2:645555/147‑1 (MQ=255)
                                                                                                                                         cAGCTTCTCGCCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc               <  1:116311/151‑1 (MQ=255)
                                                                                                                                          aGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc               >  1:197212/1‑150 (MQ=255)
                                                                                                                                            cTTCTCGCCCGCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCAt                <  2:58508/147‑1 (MQ=255)
                                                                                                                                             ttCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATc               >  1:1007917/1‑147 (MQ=255)
                                                                                                                                               cccGTCCCCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCg         <  1:234234/149‑1 (MQ=255)
                                                                                                                                                tcGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGt        >  1:380592/1‑151 (MQ=255)
                                                                                                                                                    ccACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAt     <  1:711371/150‑1 (MQ=255)
                                                                                                                                                      aCCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCAtttt  >  2:48609/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACTTTGTCGTAATCCAGCACGTCGTCCATCAGCGGTGCTGTTTTCGGCCCGACCTGAATCTTCAGCTTCTCGTCCACCCCGCCGTTAATTGCGTAGAGCAGCGTTTTCGCCAGGTTAGCGCGTGCACCAAAGAACTGCATTTGCTTACCAATCACCATTGGGCTGACGCAGCAGGCAATCGCGTAATCGTCGCTGTTGAAGTCAGTACGCATCAGATCGTCATTTT  >  NC_000913/3260906‑3261205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: