Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8800TC44.9% 64.5 / 12.4 49K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (17/10);  new base C (0/22);  total (17/32)
Fisher's exact test for biased strand distribution p-value = 1.61e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTT  >  NC_000913/357733‑358027
                                                                                                                                                         |                                                                                                                                                       
cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccg                                                                                                                                                     <  2:932311/151‑1 (MQ=255)
 gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                                                                                                                                    <  1:1062436/151‑1 (MQ=255)
 gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgc                                                                                                                                                    >  2:277874/1‑151 (MQ=255)
  tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgcc                                                                                                                                                   >  2:57539/1‑151 (MQ=255)
  tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgcc                                                                                                                                                   >  1:518240/1‑151 (MQ=255)
   aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgcc                                                                                                                                                   <  1:695897/150‑1 (MQ=255)
          aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaacaa                                                                                                                                            <  2:1144613/150‑1 (MQ=255)
          aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa                                                                                                                                           <  2:1054938/151‑1 (MQ=255)
          aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa                                                                                                                                           <  1:646499/151‑1 (MQ=255)
           tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa                                                                                                                                           >  2:192340/1‑150 (MQ=255)
               cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgc                                                                                                                                      >  1:1171240/1‑151 (MQ=255)
               cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg                                                                                                                                       <  2:1078427/150‑1 (MQ=255)
                  aGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGt                                                                                                                                   >  1:1037005/1‑151 (MQ=255)
                   gcgcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg                                                                                                                                    <  1:768133/149‑1 (MQ=255)
                         cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc                                                                                                                            >  2:408317/1‑151 (MQ=255)
                           ggCATTTCCGGCCCCAGCCCTTTGTCGGCGGCTCCCGTTTTTCTTTCCCCCAAACACCCGCGCTTTTATCCACCCCCATGTCCAAAAATCCCTTCGGGGCGGCTGTCTCCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCag                                                                                                                          <  1:1198153/151‑1 (MQ=255)
                             cattcccAGCCGCAGCGTTGTGTCAGCGGCTACCGTGTTTCTTCCCCGCCAACAACCGCGCATTTATCCACCGCCATGTGCAAAAATGCCGTCGGGGCGGCTGTCTGCCATTTTTTTTTCCCCCGCAACAAAACCGCCGTtctctc                                                                                                                             <  2:1276174/141‑1 (MQ=255)
                               aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCGGTCTGCCAGCTATTTTTCCACCGCAACAAAACCGCCGTTCTCTCCagta                                                                                                                        >  2:77627/1‑149 (MQ=255)
                                  ccccccGCAGCCTTGTGTCAGCGGCTCCCGTTTTTCATTCCCCCCACCACCCCCCCTTTTCCCCACCCCCATGTAAAAAAATCCCTCCGCGGCGGCTGCCTGCAAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCagta                                                                                                                        <  2:31752/142‑1 (MQ=255)
                                    aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                 >  1:768704/1‑151 (MQ=255)
                                      ccGAAGCCCTGTGACAGCGGCTCCGGTATTTATTTCCCCCCAACACCCGCCCTTTTACCCACCCCCTTGTGAAAAAATGCTTTCGGGGGGGCTGTCTGCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgg                                                                                                                 <  1:52819/149‑1 (MQ=255)
                                         ctgccttgtgTCGGCGGCTTCGGTGATTCATTTCCGCCAACACCCGCTCTTTTAACCACCGCTATTTAAAAAACTGCCTCGGCGGCGCCTCTCTGCGATCTATTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa                                                                                                             <  1:311717/144‑1 (MQ=255)
                                             cttgtgCCAGCGGCCCCCCTGATTCTTTCCCCCCAACACCCGCGCATTTACCCACCGCCATGTGAAAAAATGCTTCGGGGGGGCTGTTCGGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa                                                                                                            <  2:1081003/145‑1 (MQ=255)
                                             ccTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                >  1:991877/1‑143 (MQ=255)
                                                gtgtCGGCGCCTCCCGTGATTCTTTCCCCCAAACACCCGCGTTTTTACCCACCCCCTTGTAAAAAAAGCCCTCCGCGGGGGCTGTCGGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAgaga                                                                                                       <  2:113503/148‑1 (MQ=255)
                                                gtgtCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggacgtaga                                                                                                       >  2:327771/1‑141 (MQ=255)
                                                  gccAGCGGTTCCCGTGATTCTTTCCCCCCAAAACCCGCCCTTTTACCCACCCCCTTGTGAAAAAAGGCTTCCGGGGGGGCTTTCTGCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAgaga                                                                                                       <  1:1242738/144‑1 (MQ=255)
                                                         ggctcccgtttttaTTTCCCCCAAACACCCGCGAATTTTCCAACCGCCAGGTAAAAAAATCCTTCCGGGGCGCTTGTCTCCCATTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAagag                                                                                                         <  1:377618/126‑1 (MQ=255)
                                                          ctcccgtttttCTTTCCCCCCACCACCCGCCCTTTTACCCACCCCCTTTTCAAAAAATCCTTCCGCGGCGGCTTTCTCCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGcggg                                                                                              <  1:408735/138‑4 (MQ=255)
                                                          cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGaaaaaaa                                                                                                 >  1:300445/1‑140 (MQ=255)
                                                           tACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGTGGAaggaaaaaaacattta                                                                                             >  1:632960/1‑134 (MQ=25)
                                                             ccttatttCATTCCCGCCACCACCCGCGTTTTTATCCACCCCAATGTGAAAAAAGGCCTGCGGGGCGGCTTTCGGCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGccc                                                                                       <  1:1034467/144‑1 (MQ=255)
                                                                tttttCTTTCCCCCCACCACCCGCTCTTTTACCAAACCCTTTGTAAAAAAATGCCTCCGCGGGGGTTGTCTCCAGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTc                                                                                    <  2:803179/147‑1 (MQ=255)
                                                                tGATTCTTTCCCCCAAACACCCCCGATTTTACCCACCGCATTGTCAAAAATTCCCTCGGCGGGGGCTGTCGGCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGt                                                                                     <  2:1226909/149‑1 (MQ=255)
                                                                            ccccaaacaccCGCGCTTTTACCCACCCCCTTGTAAAAAAATGCCTTCGCGGCGGTTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTtgtgtg                                                                          <  1:102361/145‑1 (MQ=255)
                                                                                caaacaccccctaTTTAACCAACCGCCATTTAAAAAATTCCCTCCGGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCaa                                                                      <  1:562494/139‑1 (MQ=255)
                                                                                 accacccGCGCTTTTACCAACCCCATTGTAAAAAAATCCTTCGGGGGCGGTTGTCTGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCaa                                                                      <  2:1279175/144‑1 (MQ=255)
                                                                                        gcgcATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAAGAAAAAACATTAAAACCCTCCAGTTGTggggggaaag                                                                   >  1:133189/1‑139 (MQ=25)
                                                                                         cccttttACCCACCCCCATGGAAAAAAATCCTTCCGGGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTgg                                                            <  1:992909/147‑1 (MQ=255)
                                                                                                   cccaccgcctttagaaaaaaTCCCTTCGCGGCGGCTGTCCGCCACTTTTTTTCCCGCCGCAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt                                                    <  1:1711/135‑1 (MQ=255)
                                                                                                                  aaaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAACTTTTAACCCCTCACGTTTTGTTGGTCTCGCTCCTCGTGAACAAGTGGGAAGGGGAgag                                     >  2:167779/1‑148 (MQ=255)
                                                                                                                   aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGCGAGAAAAAAATTTTAAACCCCCCAGTTGTGTGGGGAACGCCGCTGGGGAAAAAGTGGGAAGGGGAgaggg                                   >  1:240995/1‑147 (MQ=255)
                                                                                                                    aaaTGCCTCGGGGGGGGTTTTCGGCCAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                  <  1:745586/151‑1 (MQ=255)
                                                                                                                               gggggggcTGTCGGCCGTTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCa                           <  2:1186843/143‑1 (MQ=255)
                                                                                                                               ggggcggcTGTCTGCCGTCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAg                          <  2:376923/147‑1 (MQ=255)
                                                                                                                                 ggcggcTTTCTGCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATc                            <  1:1227983/145‑1 (MQ=255)
                                                                                                                                    ggcTGTCGCCCAGTTATTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg                    <  1:364959/150‑1 (MQ=255)
                                                                                                                                       tGTCTGCCATTTTTTTTCCCGCCCCAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGa                   <  2:509482/148‑1 (MQ=255)
                                                                                                                                           cTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAACTTTTAACCCCGCCTGTTGTGTGGGAAACGCCGCTGGGAAAAAAGTGGGAAGGGGAGGGGGGGGGATCAACTCCCCAAcccc              >  1:812105/1‑148 (MQ=255)
                                                                                                                                                attttttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTaa         <  1:1080179/145‑1 (MQ=255)
                                                                                                                                                        ttCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAACATTTAACCCCGCACGTTTTGTGGGAAACGCCGCTGGTAACAAAGTGGGAAGGGGAGGGGGGGGGAAAAACCCCCGAACCGCGCCAAATGAggt  >  1:413138/1‑147 (MQ=255)
                                                                                                                                                         |                                                                                                                                                       
CGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTT  >  NC_000913/357733‑358027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: