Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,212	T→C	E273G (GAA→GGA)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,212	0	T	C	100.0%	344.2 / NA	85	E273G (GAA→GGA)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/46);  total (39/46)

GGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCC  >  NC_000913/3440065‑3440358                                                                                                                                                    |                                                                                                                                                   ggCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCt                                                                                                                                                  <  2:680617/150‑1 (MQ=255)  gCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGc                                                                                                                                                <  1:715116/151‑1 (MQ=255)  gCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGc                                                                                                                                                <  2:395084/151‑1 (MQ=255)    cAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCtt                                                                                                                                              <  2:115794/151‑1 (MQ=255)      gTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTa                                                                                                                                            <  1:235037/151‑1 (MQ=255)          tCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGc                                                                                                                                        >  1:455702/1‑151 (MQ=255)              ggCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGtt                                                                                                                                    <  1:921962/151‑1 (MQ=255)                 gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGc                                                                                                                                 >  2:999166/1‑151 (MQ=255)                   aTGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCag                                                                                                                               <  1:20149/151‑1 (MQ=255)                      cccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCaga                                                                                                                              <  2:822430/149‑1 (MQ=255)                          gagaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGAc                                                                                                                        <  1:697504/151‑1 (MQ=255)                           agaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACa                                                                                                                       <  2:390213/151‑1 (MQ=255)                           agaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACa                                                                                                                       <  2:1073194/151‑1 (MQ=255)                            gaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGAc                                                                                                                        >  1:833184/1‑149 (MQ=255)                               aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCa                                                                                                                   <  2:824555/151‑1 (MQ=255)                                gTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCaa                                                                                                                  <  1:192289/151‑1 (MQ=255)                                gTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGGGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTcg                                                                                                                   <  2:807353/150‑2 (MQ=255)                                  ccACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAAtt                                                                                                                <  1:685072/151‑1 (MQ=255)                                  ccACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAAt                                                                                                                 <  1:630077/150‑1 (MQ=255)                                   cACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTc                                                                                                               >  2:1145943/1‑151 (MQ=255)                                    aCGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                                                                                                              <  1:263078/151‑1 (MQ=255)                                     cGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                                                                                                              >  1:431089/1‑150 (MQ=255)                                     cGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCa                                                                                                             <  1:586341/151‑1 (MQ=255)                                          aGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                                                                                                              >  2:1075365/1‑145 (MQ=255)                                           gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGt                                                                                                       >  1:446956/1‑151 (MQ=255)                                               gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                   <  2:277478/151‑1 (MQ=255)                                               gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                   >  1:428215/1‑151 (MQ=255)                                                cACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACa                                                                                                  <  2:291142/151‑1 (MQ=255)                                                 aCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAAc                                                                                                   >  2:442980/1‑149 (MQ=255)                                                    tCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgc                                                                                              >  2:577603/1‑151 (MQ=255)                                                        tAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgca                                                                                           <  2:655206/150‑1 (MQ=255)                                                         aaTCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcagc                                                                                         <  2:455698/151‑1 (MQ=255)                                                           tctcAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcagc                                                                                         >  1:932595/1‑149 (MQ=255)                                                              cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggat                                                                                    <  1:35807/151‑1 (MQ=255)                                                              cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAgg                                                                                      <  1:994153/149‑1 (MQ=255)                                                                          ttttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActc                                                                        >  2:1190181/1‑151 (MQ=255)                                                                           tttACTAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAACTCCTGCTTTAAGGCAGTTAGCAGAGAGGACAGTCAATTCCAGATCGTCAAAAGGGCGCAGCAGGATCGGATCGAAct                                                                         >  2:747553/1‑149 (MQ=255)                                                                           tttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAActct                                                                       >  1:869633/1‑151 (MQ=255)                                                                            ttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTg                                                                      >  1:1003258/1‑151 (MQ=255)                                                                             tACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTg                                                                      <  2:780204/150‑1 (MQ=255)                                                                               ccAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                   <  2:517887/151‑1 (MQ=255)                                                                               ccAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                   >  2:291571/1‑151 (MQ=255)                                                                                 aaGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtc                                                                 >  1:644201/1‑151 (MQ=255)                                                                                  aGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                <  1:858187/151‑1 (MQ=255)                                                                                   ggTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtctc                                                               >  1:828082/1‑151 (MQ=255)                                                                                    gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTctct                                                              <  2:1027583/151‑1 (MQ=255)                                                                                    gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTctct                                                              >  1:770521/1‑151 (MQ=255)                                                                                      tAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtcttc                                                            >  2:950231/1‑151 (MQ=255)                                                                                      tAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtctt                                                             <  2:707528/150‑1 (MQ=255)                                                                                      tAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTAGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCtctt                                                             <  2:266054/150‑1 (MQ=255)                                                                                         gCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCttt                                                         <  2:330046/151‑1 (MQ=255)                                                                                            ttttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCAc                                                      >  1:979658/1‑151 (MQ=255)                                                                                               tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACtt                                                    <  1:453740/150‑1 (MQ=255)                                                                                               tAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTc                                                   >  2:375764/1‑151 (MQ=255)                                                                                                aaGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTc                                                   >  2:187826/1‑150 (MQ=255)                                                                                                aaGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCa                                                  <  2:716135/151‑1 (MQ=255)                                                                                                  ggAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAgg                                                <  1:84843/151‑1 (MQ=255)                                                                                                     gCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCt                                              <  2:1231511/150‑1 (MQ=255)                                                                                                      cTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTg                                             >  1:136370/1‑150 (MQ=255)                                                                                                       tCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGAc                                           >  2:1181452/1‑151 (MQ=255)                                                                                                           ccTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTAc                                       >  1:122483/1‑151 (MQ=255)                                                                                                                gTACGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCAc                                  >  1:269312/1‑151 (MQ=255)                                                                                                                  aCGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACg                                 >  2:336441/1‑150 (MQ=255)                                                                                                                  aCGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACg                                 <  1:851178/150‑1 (MQ=255)                                                                                                                   cGCTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGt                                >  1:864451/1‑150 (MQ=255)                                                                                                                     cTGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTa                               >  2:794789/1‑149 (MQ=255)                                                                                                                      tGTACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAg                             >  1:200240/1‑150 (MQ=255)                                                                                                                        tACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                           >  2:161763/1‑150 (MQ=255)                                                                                                                        tACCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                           >  2:58778/1‑150 (MQ=255)                                                                                                                         aCCAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCaa                         <  1:112432/151‑1 (MQ=255)                                                                                                                           cAGATCACCGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACg                       >  2:963214/1‑151 (MQ=255)                                                                                                                                  ccGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt                <  2:813799/151‑1 (MQ=255)                                                                                                                                  ccGATATAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt                <  1:393968/151‑1 (MQ=255)                                                                                                                                     atatAGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGTACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCCGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc              <  2:37615/150‑1 (MQ=255)                                                                                                                                      tataGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc              >  2:914218/1‑149 (MQ=255)                                                                                                                                      tataGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc              >  1:107358/1‑149 (MQ=255)                                                                                                                                      tataGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTcc              >  2:642881/1‑149 (MQ=255)                                                                                                                                         aGTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttg         <  2:79538/151‑1 (MQ=255)                                                                                                                                          gTGGATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgt        <  1:877062/151‑1 (MQ=255)                                                                                                                                            ggATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgtt       <  1:302071/150‑1 (MQ=255)                                                                                                                                            ggATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTc      >  1:759516/1‑151 (MQ=255)                                                                                                                                             gATAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCa     <  2:54661/151‑1 (MQ=255)                                                                                                                                              aTAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAg    <  2:61793/151‑1 (MQ=255)                                                                                                                                              aTAGCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAg    <  2:608532/151‑1 (MQ=255)                                                                                                                                                 gCTCCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGcc  >  1:280604/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCC  >  NC_000913/3440065‑3440358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑