Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,237	0	C	T	45.3%	85.3 / 33.5	75	S64L (TCG→TTG)	lsrD	autoinducer 2 import system permease protein
Reads supporting (aligned to +/- strand):  ref base C (9/32);  new base T (34/0);  total (43/32)
Fisher's exact test for biased strand distribution p-value = 2.49e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTA  >  NC_000913/1604088‑1604386                                                                                                                                                      |                                                                                                                                                        gCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTc                                                                                                                                                         <  2:720703/150‑1 (MQ=255)   tCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGtt                                                                                                                                                      >  1:918932/1‑151 (MQ=255)     gTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGAATTTTGttt                                                                                                                                                     >  1:84631/1‑150 (MQ=255)       tATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTgg                                                                                                                                                   <  1:1288542/150‑1 (MQ=255)       tATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTgg                                                                                                                                                   <  2:817852/150‑1 (MQ=255)        aTTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGttt                                                                                                                                                     >  2:723001/1‑147 (MQ=255)        aTTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGt                                                                                                                                                  <  2:856451/150‑1 (MQ=255)        aTTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGt                                                                                                                                                  <  2:1122426/150‑1 (MQ=255)                      aTTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTTGTTCGACCATCGG‑cc                                                                                                                                    >  2:274867/1‑149 (MQ=255)                       tttGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTGGTTCTAccccccg                                                                                                                                       >  1:963858/1‑141 (MQ=255)                          ggTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTgcg                                                                                                                             <  1:113564/151‑1 (MQ=255)                          ggTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTgcg                                                                                                                             <  2:1037403/151‑1 (MQ=255)                              cAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGGCCATCGG‑CCT‑CTTCGcc                                                                                                                           >  2:142895/1‑149 (MQ=255)                               aaTTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGAGCGGGATTTTGTTTGGTTGGACCCTCCG‑cccgcgggccatt                                                                                                                         >  1:271696/1‑140 (MQ=255)                                  tAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTCTTTTTTTTCCACCACCCG‑Ccg‑ctcagcccc                                                                                                                         >  2:279129/1‑140 (MQ=255)                                   aaCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCcattgcat                                                                                                                    <  2:748453/151‑1 (MQ=255)                                    aCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTTTTTTTTTTTGTTTCCCCCCCGG‑GCT‑CTCCGGcattgtact                                                                                                                   >  1:955435/1‑147 (MQ=255)                                    aCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGAGGGTATTTTTTTTTGTTTGAACAACGG‑CCC‑CTGCGCcattgcct                                                                                                                    >  1:1318691/1‑148 (MQ=255)                                    aCCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGATATTTTCTTTGGTTTGGCCCTAGG‑CCT‑CTTCGCccttggctt                                                                                                                   >  1:94837/1‑147 (MQ=255)                                      ccGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTgg                                                                                                                 <  1:859720/151‑1 (MQ=255)                                          gAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTTGACCATCGG‑CCT‑CTGCGCCATTGCATTggggg                                                                                                              >  1:396713/1‑148 (MQ=255)                                           aaTGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGGTATTTTGTTTTGTTTGACCATCCG‑CCT‑CCGCGCCATTTCATTTGGCg                                                                                                              >  1:51321/1‑149 (MQ=255)                                           aaTGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGATATTTTGTTTTGTTTGACCATCGG‑CCC‑CTGCGCCCTTGCCTTGGGCg                                                                                                              >  1:262308/1‑149 (MQ=255)                                                 aGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGtt                                                                                                       <  2:488677/150‑1 (MQ=255)                                                    tctcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATTGATATTTTGTTTTGTTTGACCATCCG‑CCT‑CTGCGCCCTTGCATTTGGCGGACTGttt                                                                                                      >  1:1176075/1‑148 (MQ=255)                                                     ctcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGTTTGACCATCCG‑CCT‑CTGCGCCCCTGCATTTGGCGGACTGTTTTaa                                                                                                   >  1:523470/1‑150 (MQ=255)                                                     ctcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTTGTTTGACCATCGG‑CCT‑CTGCGCCCTTTCATTGGGGGTACTGttt                                                                                                      >  1:105792/1‑147 (MQ=255)                                                     ctcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTGGGTTCACCATCGG‑CCT‑CCGCGCCCATGGGTTGGGGGTTCTGttt                                                                                                      >  1:1261711/1‑147 (MQ=255)                                                      tcAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTTTTTTGGTCGACCATCGG‑CCT‑CTGCGCCCCTTCCTTTGGGGTACTGttttta                                                                                                   >  1:1160896/1‑146 (MQ=255)                                                       cAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGGGTACTGTTTTaaa                                                                                                  >  2:676265/1‑149 (MQ=255)                                                       cAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAgt                                                                                                <  2:1232387/151‑1 (MQ=255)                                                        aaTATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCCTTGCATTGGGGGTACTGTTTTAAAgtg                                                                                               >  1:287969/1‑151 (MQ=255)                                                         atatGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTTGTTCGACCATCGG‑CCT‑CTGCGCCCTTGCATTTGGCGGACTGTTTTaaaaagg                                                                                              >  2:165064/1‑147 (MQ=255)                                                              ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGGTATTTCGTTTGGTTCCACCATCGG‑CCC‑CTGCGCCCTTTCCTTTGGCGTACTGTTTTTAAGAGGggtg                                                                                          >  1:275542/1‑150 (MQ=255)                                                              ttGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGG‑CCC‑CTGCGCCCTTTCATTGGGCGTACTGTTTTAAAgtggtg                                                                                            >  1:676274/1‑148 (MQ=255)                                                                 cTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTGTTTTGGTCGACCATCCG‑GCT‑CTGGCCCCTTTCATTTGGCGTTCTTTTTTTCAgtggtg                                                                                            >  1:760969/1‑145 (MQ=255)                                                                 cTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGCTATTTTGTTTGGTTCGAACATCCG‑CCC‑CTGTGCCCTTGCATTGGGGGGCCTGTTTCCAAGTGGTGTGCCgt                                                                                      >  2:33390/1‑150 (MQ=255)                                                                 cTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGG‑CCT‑CTTCGCCCTTTCATTGGGCGGACTGTTTTTAAgtgg                                                                                              >  2:417615/1‑143 (MQ=255)                                                                      cAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGcc                                                                                  <  1:811069/150‑1 (MQ=255)                                                                       aGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGGTTGATATTTTTTTTTGTTCTGCCCTCCG‑CCT‑CCGCGCCCTTGCATTTGGGGTACTGTTTTTTAGAGGggtgccggtgcc                                                                                  >  1:1002993/1‑149 (MQ=255)                                                                        gCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGCTTGATATTTCTGTTGGTTCGGCCCTTTG‑CCTCCTCCGCCA‑TGCACTGGGGGGTCTGTTTTAAAGTGGTGTGCCCGTGCCGc                                                                                >  2:593106/1‑150 (MQ=255)                                                                        gCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCt                                                                               <  1:1334386/151‑1 (MQ=255)                                                                          aCCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGGACTGTTTTAAAGTGGTGTGCCGATGCCGc                                                                                >  2:1234487/1‑148 (MQ=255)                                                                              gTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGAt                                                                         <  1:221875/151‑1 (MQ=255)                                                                                gACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATAc                                                                       <  1:301892/151‑1 (MQ=255)                                                                                    ttATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTGGGTCGACCATCGG‑CCT‑CTTCGCCCTTGCATTGGGCGTACTGTTTCAAAGTGGGGTGGCCAAGCCGCCGGCGATACTcc                                                                    >  2:804956/1‑150 (MQ=255)                                                                                      aTCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTg                                                                  <  1:296048/150‑1 (MQ=255)                                                                                           cattggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTa                                                            <  1:693476/151‑1 (MQ=255)                                                                                            attggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTTGTTTTGTTTGCACATCGG‑CCT‑CTTCGGCCTTTCATTGGGCGTTCTGTTTTAAAGGGGGTTGGCGATGGCGCTGGGGGAACTCCTGACCtt                                                             >  2:613127/1‑149 (MQ=255)                                                                                               ggcattgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGTATTTTGTTTGGTTCGACCCTCGG‑CCC‑CTGCGCCCTTGCATTGGGCGGACTGTTTCTAAGTGGTGGGGCGCGGCCGCCGGCGGTACTCCTGACCTTTCt                                                          >  1:1025954/1‑149 (MQ=255)                                                                                                   ttgTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGAAGGAGATTTTGTTTTGGTTGACCCACGG‑CCC‑CCGCCCCAATGCATTGGGGGGACGGTTTTGAAGTGGTGGGCCGCTGCCGCGGGCGGTACTTCTGCCCCTACTGCTCgg                                                    >  2:240978/1‑151 (MQ=255)                                                                                                         cccTACCGCTAACGATGGTGATTGTCAGTGGCGGGGGCGATATTTTTTTTTGGTTGACCATAGGCCCC‑CTCGGCCTTTGC‑TTGGGGGGTCTGTTTTAAAGAGGGGGGGCCATGCCCCCGGCGGGACTCCtttcccttc                                                           >  1:372539/1‑130 (MQ=255)                                                                                                          ccTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCAACGG‑CCT‑CTGCGCCATTGCATTGGGCGGACTGTTTTAAAGGGGGGTGGCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCCGCGCagt                                              >  2:1374744/1‑148 (MQ=255)                                                                                                           cTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGGAATTTTGTTTTGTTCGACCATCCG‑CCC‑CTTCGCCATTGCAATGGGCGGACTGTTTCAAAGTGGGGTGGCGGTGCCGCCGGCGGTACTCCTGACCTTACTGCCCCGCGCAtt                                              >  2:566583/1‑149 (MQ=255)                                                                                                             aCCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTTCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATtgtg                                           <  2:599080/150‑1 (MQ=255)                                                                                                             aCCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGc                                          <  1:637913/151‑1 (MQ=255)                                                                                                              ccGCTAACGATGGTGATTGTCAGTGGCGGGGTCGCTATTTCCTTTTGGTCCGCCATCGG‑GCC‑CTGCGCCCTTGGCTTGGGCGTACTGTTTCAAAGTGGTGGGCCGGTGCCGCCGGCGGTACTCCCGGCCCTACATCTCGGCGGCTtgtggg                                         >  2:823445/1‑149 (MQ=255)                                                                                                              ccGCTAACGATGGTGATTGTCAGTGGCGGGGTCGATATTTTTTTTTGTTTGACCATCGG‑CCT‑CTGCGCCATTGCCTTGGGCGGACTGTTTTTAAGTGGTGTGGCGATGCCGCTGGCGGTACTCCTGACCTTTCTGCTCGGCGCAtt                                              >  2:336347/1‑146 (MQ=255)                                                                                                                   aaCGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGc                                      <  2:259698/149‑1 (MQ=255)                                                                                                                    aCGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGa                                   <  2:1054300/151‑1 (MQ=255)                                                                                                                       aTGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCa                                <  1:638506/151‑1 (MQ=255)                                                                                                                            gATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGcc                           <  1:888682/151‑1 (MQ=255)                                                                                                                            gATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGcc                           <  1:1305302/151‑1 (MQ=255)                                                                                                                                  cAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGatta                     <  2:863936/151‑1 (MQ=255)                                                                                                                                   aGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGattaa                    <  2:955443/151‑1 (MQ=255)                                                                                                                                    gTGGCGGGATCGGTATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGattaa                    <  1:1246967/150‑1 (MQ=255)                                                                                                                                      ggCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGattaatt                  >  2:489008/1‑150 (MQ=255)                                                                                                                                       gCGGGATCGATATTTCGTTTGGTTCGACCAGCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATTCTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTAt                <  2:529687/151‑1 (MQ=255)                                                                                                                                         gggATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCt              <  2:84631/151‑1 (MQ=255)                                                                                                                                          ggATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCta             <  2:992028/151‑1 (MQ=255)                                                                                                                                           gATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCtat            <  2:1869/151‑1 (MQ=255)                                                                                                                                              cGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATAcc         >  1:14026/1‑151 (MQ=255)                                                                                                                                                 tatTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCa        >  1:333965/1‑149 (MQ=255)                                                                                                                                                  atTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCaaa      <  1:676262/150‑1 (MQ=255)                                                                                                                                                  atTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCaaa      <  2:1261722/150‑1 (MQ=255)                                                                                                                                                  atTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCaa       >  1:1353195/1‑149 (MQ=255)                                                                                                                                                      cGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTa  <  1:47624/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        GCTCGTTATTGAGATTGTCGCATTTGGTGCAATTAACCCGCGAATGTTAGATCTCAATATGTTGCTGTTCAGCACCAGTGACTTTATCTGCATTGGCATTGTCGCCCTACCGCTAACGATGGTGATTGTCAGTGGCGGGATCGATATTTCGTTTGGTTCGACCATCGG‑CCT‑CTGCGCCATTGCATTGGGCGTACTGTTTCAAAGTGGTGTGCCGATGCCGCTGGCGATACTCCTGACCTTACTGCTCGGCGCATTGTGCGGGCTGATCAACGCCGGATTAATTATCTATACCAAAGTTA  >  NC_000913/1604088‑1604386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑