Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913354,2880TG40.0% 138.1 / 27.3 80V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (10/38);  new base G (32/0);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 9.15e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437
                                                                                                                                                    |                                                                                                                                                     
ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTg                                                                                                                                                      <  2:701934/149‑1 (MQ=255)
    cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGAt                                                                                                                                                <  1:500848/151‑1 (MQ=255)
       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                             <  1:836290/151‑1 (MQ=255)
       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc                                                                                                                                              <  1:818792/150‑1 (MQ=255)
       ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGaag                                                                                                                                               >  1:1232324/1‑147 (MQ=255)
           gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGa                                                                                                                                         <  2:392570/151‑1 (MQ=255)
             cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaaa                                                                                                                                       <  2:986685/151‑1 (MQ=255)
                      ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGACGCGCTGAAAAGGCAggtg                                                                                                                              >  2:737257/1‑151 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                              <  2:1203641/150‑1 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                              <  2:548442/150‑1 (MQ=255)
                       tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCTGGAAgggggggggg                                                                                                                             >  1:969126/1‑144 (MQ=255)
                        aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggc                                                                                                                            <  1:187103/151‑1 (MQ=255)
                        aTCACTTTATTCTCGGGCGCACTGACGATGCAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAGGCGCGGAAAggggcggggg                                                                                                                             >  1:564872/1‑143 (MQ=255)
                         tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGCTGGAAGGGGAggggggg                                                                                                                           >  1:524119/1‑149 (MQ=255)
                          cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                           <  1:507310/150‑1 (MQ=255)
                                   ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGAAAAAGGGCTGGAAGGGGAGGGGGGGGGGGGGttt                                                                                                                  >  1:347148/1‑150 (MQ=255)
                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGttt                                                                                                                  <  1:1153578/148‑1 (MQ=255)
                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                                <  2:1285616/150‑1 (MQ=255)
                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                                <  1:159608/150‑1 (MQ=255)
                                     cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGt                                                                                                                <  1:786639/150‑1 (MQ=255)
                                      gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGGTGGGGGGGAAGAAGCGCTGGAAGGGCAGGGGGGGGGGGCGttt                                                                                                                  >  2:454438/1‑147 (MQ=255)
                                       ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAt                                                                                                             <  2:518045/151‑1 (MQ=255)
                                        gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                            <  1:1196056/151‑1 (MQ=255)
                                         cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCAtt                                                                                                            >  2:695289/1‑150 (MQ=255)
                                           cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCg                                                                                                         <  1:668835/151‑1 (MQ=255)
                                           cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGGGGGGGCGTTTGTCATTCCg                                                                                                         >  2:1215885/1‑151 (MQ=255)
                                              tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTTGATGGGGGGAAAGATGGGCCTAAACGGCGATTGGCGGTGGCGTTTggtcttaccc                                                                                                        >  1:1259451/1‑140 (MQ=255)
                                                    tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGAAAGGGGAGGGGGCGGGGGCGGTTTTCATTTCGaaa                                                                                                      >  2:1249987/1‑145 (MQ=255)
                                                    tGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGGTTGTCATTCCGAAagagag                                                                                                 >  2:1372499/1‑150 (MQ=255)
                                                     gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGc                                                                                                <  1:1067344/150‑1 (MQ=255)
                                                     gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                               <  1:675775/151‑1 (MQ=255)
                                                       aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGa                                                                                              <  1:228495/150‑1 (MQ=255)
                                                        aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                            <  1:1203143/151‑1 (MQ=255)
                                                        aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAAATGCGCCGGAAGGGGAGGGGGCGGGGGGGGTTGTCATTCCGGAAAAGAGCg                                                                                               >  1:345124/1‑148 (MQ=255)
                                                         ttAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCg                                                                                               >  2:857536/1‑147 (MQ=255)
                                                            aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGGGGGGGCGGTTGTCATTTCGAAAGAGAGCg                                                                                               >  2:1298137/1‑144 (MQ=255)
                                                              gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                      <  1:1352761/151‑1 (MQ=255)
                                                                  ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGAAGCGCTGGAAGGGGAGGGGGCGGGGGGGGTTTTCATTTCGAAAAAGAGCGGCAGGCTgg                                                                                      >  1:1364238/1‑147 (MQ=255)
                                                                     ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                               <  1:1277726/151‑1 (MQ=255)
                                                                        aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGCCGGAAGGGGAGGGGGGGGGGGGGGTTTTCCTTTCGCAAGAGAGGGGCAGGCTGGGAGATCg                                                                               >  1:1229221/1‑148 (MQ=255)
                                                                          cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCggg                                                                             >  2:1265914/1‑146 (MQ=255)
                                                                           ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGGAAGAAGGGGTGGAAGGGCGGAGGGGTGGGGGGTTTGGCATTTCGCAAGAGAGCggtggcgtggaag                                                                                   >  1:743904/1‑129 (MQ=255)
                                                                             cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCCGAAAGGGGAGGGGGGGGGGGCGGTTGTCATTTCGCAAGAGAGCGAAAGTCTGGGAGAACGGGATgggg                                                                       >  1:842271/1‑151 (MQ=255)
                                                                             cTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGGGCTGAAAGGGGAGGGGGGGGGGGGGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGGTgggg                                                                       >  1:196385/1‑151 (MQ=255)
                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                                      <  1:763516/150‑1 (MQ=255)
                                                                               ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                                      <  2:28356/150‑1 (MQ=255)
                                                                                 ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCa                                                                   <  1:435167/151‑1 (MQ=255)
                                                                                  gACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGCGGTGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGCAc                                                                  >  1:846231/1‑151 (MQ=255)
                                                                                    cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTCGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGCCAGTCTGGAAGATCGTGATGTGGCGCACTc                                                                <  1:332972/151‑1 (MQ=255)
                                                                                     gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                               <  1:152016/151‑1 (MQ=255)
                                                                                        cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAAAGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGGCAGGCCGGGAGATCGTGGTGGGGCGCCCTc                                                                >  1:55120/1‑147 (MQ=255)
                                                                                        cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTc                                                                >  2:1356480/1‑147 (MQ=255)
                                                                                         gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                           <  1:910263/151‑1 (MQ=255)
                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAAATGCGCCGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGGGGCGCACTCGc                                                              >  1:625660/1‑147 (MQ=255)
                                                                                          agaTTGAAGAGAGTATCTCCAGCCATCCGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGcca                                                            >  2:651054/1‑146 (MQ=255)
                                                                                           gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAagag                                                         <  2:538830/151‑1 (MQ=255)
                                                                                             ttGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGAAAGATGCGCTGAAAGGGCCGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTCGAAGATTGTGGTGTGGCGCCCTCGCCagag                                                         >  2:224680/1‑149 (MQ=255)
                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAgg                                                     <  2:302071/151‑1 (MQ=255)
                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  1:860469/150‑1 (MQ=255)
                                                                                               gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                                      <  2:413995/150‑1 (MQ=255)
                                                                                                aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGACAAATGCTCTGAGAGGGCGGGGGGCGGTGGGGGTTGTTCTTCCCTAAAAGAGCGGAAGTCTGGAAGATAGTGAGGGGGTGCCCTCCCCagag                                                         >  1:232761/1‑146 (MQ=255)
                                                                                                     agTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGGAAGAGAGCGACAGGCTGGGAGAACGGGGTGTGGGGCAATCGCAAGAGaaaggg                                                   >  1:595739/1‑143 (MQ=255)
                                                                                                      gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                              <  1:135759/151‑1 (MQ=255)
                                                                                                        aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                             <  1:1142803/150‑1 (MQ=255)
                                                                                                          ctcCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGAAAGATGCGCTGGAAAGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGTGATGTTGCGCACTCGCAAGAGAAAGGGATTATTgcg                                          >  2:835990/1‑151 (MQ=255)
                                                                                                             cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtg                                       <  1:970294/151‑1 (MQ=255)
                                                                                                                 cATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGCGGGGGCGGTTGTTATTTCCAAAGAGAGCGACAGGCTGGAAGATTGGGATGTTGCGCACTCGCAAGAGAAGGGGATTAAGGGGCtggggg                                   >  2:605708/1‑151 (MQ=255)
                                                                                                                  aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                   >  2:846648/1‑150 (MQ=255)
                                                                                                                      gggCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCCGAAAGGGCAGGGGGCGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGCGCACTCGCAAGAGAAGGGGGTTATGGCGCTGGTGGACa                                >  1:785792/1‑149 (MQ=255)
                                                                                                                         cGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAAGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTGATTCCGAAAGAGAGAGACAGTCTGGAAGAACGGGGTGGGGCGCACTCGCCAGAGAAGGGGATTATGGCGCTGGTGGACAGcc                             >  1:238271/1‑149 (MQ=255)
                                                                                                                         cGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGGTTATGGCGCTGGTGGACAGcc                             >  2:896746/1‑149 (MQ=255)
                                                                                                                            tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                         <  1:857528/150‑1 (MQ=255)
                                                                                                                                 aaGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCaa                   <  2:336266/151‑1 (MQ=255)
                                                                                                                                    tGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt                >  1:201661/1‑151 (MQ=255)
                                                                                                                                      gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg              >  1:869392/1‑151 (MQ=255)
                                                                                                                                        ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc            >  2:76468/1‑151 (MQ=255)
                                                                                                                                         gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  2:1209740/1‑151 (MQ=255)
                                                                                                                                         gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc           >  2:881068/1‑151 (MQ=255)
                                                                                                                                          tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg          >  2:605898/1‑151 (MQ=255)
                                                                                                                                                   gTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcgc  >  1:923786/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCGC  >  NC_000913/354141‑354437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: