Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,675	Δ1 bp	coding (1219/1374 nt)	mdtK →	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,674	0	G	.	100.0%	259.9 / NA	54	coding (1218/1374 nt)	mdtK	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (33/21);  total (33/21)

GCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGC  >  NC_000913/1744537‑1744820                                                                                                                                          |                                                                                                                                                   gcggcgGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCt                                                                                                                                      <  1:146523/151‑1 (MQ=255)      ggTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTc                                                                                                                                  <  2:571467/150‑1 (MQ=255)         atatCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGtt                                                                                                                               <  1:326540/150‑1 (MQ=255)         atatCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGtt                                                                                                                               <  2:577507/150‑1 (MQ=255)               gATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCt                                                                                                                         <  2:484397/150‑1 (MQ=255)                  ttCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATgg                                                                                                                     >  2:447801/1‑151 (MQ=255)                      gACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGc                                                                                                                  <  2:63743/150‑1 (MQ=255)                                 ggTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCt                                                                                                      <  1:511491/151‑1 (MQ=255)                                       tGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATa                                                                                                 <  1:692719/150‑1 (MQ=255)                                              gggATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttat                                                                                         >  1:749974/1‑151 (MQ=255)                                               ggATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt                                                                                        <  2:124839/151‑1 (MQ=255)                                               ggATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTttatt                                                                                        >  2:116903/1‑151 (MQ=255)                                                    ttGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTgg                                                                                   >  2:83408/1‑151 (MQ=255)                                                    ttGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTg                                                                                    <  1:740758/150‑1 (MQ=255)                                                     tGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGc                                                                                  >  1:601138/1‑151 (MQ=255)                                                      gCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGc                                                                                  <  1:95339/150‑1 (MQ=255)                                                       cGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGcc                                                                                 >  2:196313/1‑150 (MQ=255)                                                       cGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCt                                                                                >  2:598414/1‑151 (MQ=255)                                                          ggTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGAc                                                                             <  2:134850/151‑1 (MQ=255)                                                           gTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACg                                                                            >  2:286487/1‑151 (MQ=255)                                                            ttATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGt                                                                           >  1:634732/1‑151 (MQ=255)                                                                aaaGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGc                                                                       >  2:803710/1‑151 (MQ=255)                                                                  aGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGtacgc                                                                       >  1:627085/1‑145 (MQ=255)                                                                     tACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGcc                                                                   >  2:873711/1‑150 (MQ=255)                                                                      aCGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAt                                                                 >  2:170450/1‑151 (MQ=255)                                                                       cgcgTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAtt                                                                >  2:6357/1‑151 (MQ=255)                                                                        gcgTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCAtt                                                                >  1:688438/1‑150 (MQ=255)                                                                             ccATTTTCTATATTACCTTTACGGCTTACGGGGTGCTGGGCTTGCCAAGCGGGTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgat                                                           <  1:810387/150‑1 (MQ=255)                                                                              cATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatg                                                          >  2:556841/1‑150 (MQ=255)                                                                               aTTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTatgatgat                                                        >  2:76684/1‑151 (MQ=255)                                                                                  ttCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGtt                                                     >  1:495617/1‑151 (MQ=255)                                                                                      atatTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTg                                                    <  1:37339/148‑1 (MQ=255)                                                                                               ttACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGt                                          <  1:254057/149‑1 (MQ=255)                                                                                               ttACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTc                                        >  2:819052/1‑151 (MQ=255)                                                                                                 aCGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCt                                      >  2:126943/1‑151 (MQ=255)                                                                                                  cGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTg                                     <  1:812432/151‑1 (MQ=255)                                                                                                   ggCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGc                                    >  1:208383/1‑151 (MQ=255)                                                                                                    gCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGc                                    >  1:941441/1‑150 (MQ=255)                                                                                                      ttACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAAc                                 >  2:70705/1‑151 (MQ=255)                                                                                                        aCTGGGTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACg                                <  1:658823/150‑1 (MQ=255)                                                                                                             gTGCTGGGCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGcc                          >  2:321058/1‑151 (MQ=255)                                                                                                                   ggCTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGACGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGc                    >  2:599815/1‑151 (MQ=255)                                                                                                                     cTTGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGcc                   >  2:664676/1‑150 (MQ=255)                                                                                                                      ttGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGcc                   <  1:20262/149‑1 (MQ=255)                                                                                                                      ttGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCcat                 >  2:311223/1‑151 (MQ=255)                                                                                                                       tGCCAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCcatc                >  1:772581/1‑151 (MQ=255)                                                                                                                         ccAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCcatcat              >  2:44148/1‑151 (MQ=255)                                                                                                                          cAAGCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCAtt             <  1:93557/151‑1 (MQ=255)                                                                                                                             gCGGCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTg          >  2:616794/1‑151 (MQ=255)                                                                                                                               ggCTATATTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGc         >  2:496223/1‑150 (MQ=255)                                                                                                                                  tataTTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACg     >  2:805711/1‑151 (MQ=255)                                                                                                                                   atatTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAc      <  2:569158/149‑1 (MQ=255)                                                                                                                                     atTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAg   <  2:346600/150‑1 (MQ=255)                                                                                                                                     atTCT‑GCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGc  >  1:371028/1‑151 (MQ=255)                                                                                                                                          |                                                                                                                                                   GCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGC  >  NC_000913/1744537‑1744820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑