Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,465,1670GT40.5% 109.2 / 47.9 74C29F (TGT→TTT) gspMgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base G (11/33);  new base T (30/0);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 7.15e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAA  >  NC_000913/3465019‑3465314
                                                                                                                                                      |                                                                                                                                                    
tCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTttg                                                                                                                                                      <  2:323627/149‑1 (MQ=255)
 ccGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTttgttg                                                                                                                                                   <  1:98687/151‑1 (MQ=255)
  cGATGGAAAAAACAGAAAAAGAGCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTttgtt                                                                                                                                                    <  2:641016/149‑1 (MQ=255)
      ggAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCtt                                                                                                                                               <  1:67697/150‑1 (MQ=255)
      ggAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTg                                                                                                                                              <  1:834029/151‑1 (MQ=255)
       gAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCTCCGCGGTTTTTTTTGGCtt                                                                                                                                               >  2:359393/1‑149 (MQ=255)
        aaaaaaCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCCCCGCGGTTTTTTTTTGGTTggg                                                                                                                                            >  1:730477/1‑151 (MQ=255)
         aaaaaCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCTCCGGGGTTTTTTTTGGGTTgggg                                                                                                                                           >  2:483312/1‑151 (MQ=255)
          aaaaCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCCGCGTTTTTTGTTTGGTTgggg                                                                                                                                           >  2:38083/1‑150 (MQ=255)
            aaCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTTCCTCGGCGGTTTTTTTTTGGTTggggggg                                                                                                                                        >  1:927834/1‑148 (MQ=255)
             aCAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTTTTGGGTTgggggg                                                                                                                                         >  2:414821/1‑147 (MQ=255)
              cAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATa                                                                                                                                      <  2:237222/151‑1 (MQ=255)
                gAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATaaa                                                                                                                                    <  2:595407/151‑1 (MQ=255)
                 aaaaaGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCCCCGCGGTTTTTTTTTGGTTGGGGGGGTaaa                                                                                                                                    >  1:604997/1‑150 (MQ=255)
                      gATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTTGCCCGGCGGTTTTTTTTTGGTTGGGGGGGGAAAAACga                                                                                                                               >  1:941783/1‑150 (MQ=255)
                         ccGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCgataga                                                                                                                           <  2:579856/151‑1 (MQ=255)
                           ggTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCTCGGCGTTTTTTGTTTGGTTGGGGGGGTAAAAACgataga                                                                                                                           >  1:581781/1‑149 (MQ=255)
                            gTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCTCGGCGTTTTTTTTTTGGTTTGGGGGGTAAAAACgataaaaa                                                                                                                         >  2:944620/1‑146 (MQ=255)
                              cAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCCCGGCGTTTTTTTTTTGGTTGGGGGGGTAAAACCGAAAAATAc                                                                                                                        >  1:474063/1‑149 (MQ=255)
                              cAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCt                                                                                                                      <  2:74300/151‑1 (MQ=255)
                              cAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCt                                                                                                                      <  1:773761/151‑1 (MQ=255)
                              cAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGGTTTTTTTTGGCTTGGGGGGATAAAAACgatagataac                                                                                                                       >  2:471874/1‑148 (MQ=255)
                              cAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCCCGGCGGTTTTTTTTTGGCTGGGGGGATAAAAACgatagataac                                                                                                                       >  2:554779/1‑148 (MQ=255)
                                gtggtgTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTAc                                                                                                                    <  2:912482/151‑1 (MQ=255)
                                gtggtgTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTAc                                                                                                                    <  1:504706/151‑1 (MQ=255)
                                 tggtgTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCCCTCGGCGGTTTTTTTTTGGTTTGGGGGATAAAAACGATAGATAcc                                                                                                                       >  1:550472/1‑147 (MQ=255)
                                   gtgtATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACAtt                                                                                                                 <  2:658498/151‑1 (MQ=255)
                                     gtATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTg                                                                                                                <  1:20101/150‑1 (MQ=255)
                                       aTGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCa                                                                                                              <  2:667459/150‑1 (MQ=255)
                                          gACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTTTTGGCTTGGGGGGGTAAAAACGATAGATACCTACATTGCag                                                                                                             >  2:590897/1‑148 (MQ=255)
                                          gACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCCCGGCGTTTTTTTTTTGGCTTGGGGGATAAAAACGATAGATACCCACAATga                                                                                                               >  2:644862/1‑145 (MQ=255)
                                          gACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCCCCGGGGTTTTTTTTGGGTTGGGGGGGTAAAACCGATAGAGACCCACATTGCtga                                                                                                            >  1:709040/1‑149 (MQ=255)
                                           aCAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCa                                                                                                         <  1:50280/151‑1 (MQ=255)
                                              gTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCa                                                                                                      <  1:457512/151‑1 (MQ=255)
                                              gTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCCCGGGGGTTTTTTTTTGGTTGGGGGGGTAAAACCGGTAGAAAACTACATTTCAGAGCATc                                                                                                       >  2:410393/1‑150 (MQ=255)
                                                     aCTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCAc                                                                                               <  1:621242/151‑1 (MQ=255)
                                                      cTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCCCTCGGGGTTTTTTTTTTGGTTGGGGGGATAAAAACCATAAATAACTACATTTTAGAGCATCCGGccc                                                                                                 >  1:839211/1‑148 (MQ=255)
                                                       tCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCTCTCCGCGGTTTTTTTTTGGTTGGGGGGATAAAAACGAAAAATAACTACATTTCAGAGCATCAGTccc                                                                                                 >  1:769934/1‑147 (MQ=255)
                                                              aaTGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCaaaaa                                                                                     <  1:38082/151‑1 (MQ=255)
                                                              aaTGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCaaaaa                                                                                     <  2:40485/151‑1 (MQ=255)
                                                                   ttAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCCCCGCGTTTTTTTTTTGCTTGGGGGGGTAAAAACGATAGATACCCACAATTCAGAGCATCAGTCCCCCGCGCAAAAAAtt                                                                                  >  1:565621/1‑149 (MQ=255)
                                                                     aaaTCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTTTTGGCTTGGGGGGGTAAAAACGATAGAAACCTACATTTCAGAGCATCAGTCCCACGCGCAAAAAAtt                                                                                  >  2:160974/1‑147 (MQ=255)
                                                                      aaTCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTaagaa                                                                             <  1:112271/151‑1 (MQ=255)
                                                                               gggCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCCCCCGGCGTTTTTTTTTGGGTTTGGGGGGTAAAAAACATAGAAACCTACATTTCAGAGGATCAGTTCCCCCCGCCAAAAAATAAAAAAGaaaac                                                                      >  1:531802/1‑145 (MQ=255)
                                                                                  cAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGGTCTCTCGCTCGGCGGTTTTTGTTGGCTTTGGGGGGTAAAAACGGAAGATAACCACCTTGTAGAGCATCAGTCCCACCCGCAAAAAAATAAAAAAGACATCaaa                                                                   >  1:765491/1‑149 (MQ=255)
                                                                                   aGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCTCGGCGTTTTTTGTTTGCTTTGGGGGGTAAAACCGATAGATACCTACATTTCAGAGCATCAGTCCCACGCGCAAAAAAATAAAAAGGACATCaaa                                                                   >  2:136377/1‑148 (MQ=255)
                                                                                   aGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTATCTCCCCCGGCGGTTTTTTTTTGGTTGGGGGGGTAAAAACGATAAATACCTACATTTCCAGGGAACAGTCCCACCCGGAAAAAAATAAAAAAGAAAACaaa                                                                   >  1:601284/1‑148 (MQ=255)
                                                                                     aaaaaTCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGAt                                                              <  2:785764/151‑1 (MQ=255)
                                                                                         aTCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCTCGGCGGTTTTTTTTGGCTTTGGGGGGTAAAAACGATAGAAAACTACATTTCAGAGCATCAGTCCCACGCGCAAAAAAATAAAAAGGAAAACAAAAGGAAGc                                                            >  1:774522/1‑149 (MQ=255)
                                                                                           ccACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAgg                                                         <  1:445931/150‑1 (MQ=255)
                                                                                              caTCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCa                                                     <  1:301822/151‑1 (MQ=255)
                                                                                               aTCGGAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCTCGGCGGTTTTTTTTTGGTTGGGGGGGTAAAAACGATAGATACCTACATTTCAGAGCATCAGTCCCCCGCGCCAAAAAATAAAAAGGACATCAAAATGATGCAgg                                                         >  2:392955/1‑146 (MQ=255)
                                                                                                 cGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGc                                                  <  2:16765/151‑1 (MQ=255)
                                                                                                  ggAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCCCTCCGCGGTTTTTTTTGGTTTTGGGGGTTAAAAACCAAAGAAAACTACAATGCCGAGCATCAGTCCCCCGCGCAAAAAAATAAAAAAGACATTAAAAGTATTCAAGATc                                                      >  2:882367/1‑146 (MQ=255)
                                                                                                   gAAAAACAGATCGTTGCGGCACTGGCGGTTTTCTCGCTCGGCGGTTTTTGTTGGCTTGGGGGGATAAAACCGAAAGATACCTACATTGCAGAGCAACAGTCCCACGCGCAAAAAAATAAAAAAGAAATCAAATGGATgga                                                           >  2:478937/1‑138 (MQ=255)
                                                                                                   gAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGa                                                <  2:124984/151‑1 (MQ=255)
                                                                                                      aaaCAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCa                                             <  2:517345/151‑1 (MQ=255)
                                                                                                       aaCAGATCGTTGCGGCACTGGCGGTTCTCTCGCCCGGCGGTTTTTTTTGGGCTGGGGGGATAAAACCCACAGATACCCACATTGCCGAGCATCCGTCCCACACGCAAAAAATTAAAAAGGACAACAAAAGGATTCAGGGACAAACGAg                                               >  2:215703/1‑148 (MQ=255)
                                                                                                         cAGATCGTTGCGGCACTGGCGGTTTTCTCGCCCGGCGGTTTTTTTTGGGTTGGGGGGGTAAAAACGATAGATAACTACATTTCAGAGCATCCGTCCCCCCCCCCAAAAAATAAAAAGGAAATCAAATGGATGCAGGATCCAACGAGCAccc                                          >  2:739980/1‑151 (MQ=255)
                                                                                                           gATCGTTGCGGCACTGGCGGTTCTCTCCCTCGGCGGTTTTTTTTTGGTTGGGGGGGTAAAAACGATAGATACCTACATTTTAGAGGCTCCGTCCCCCCCGCCAAAAAATAAAAAAGAAATCAAAATGATGCAAGAGCAAACGAGCAccc                                          >  1:492827/1‑149 (MQ=255)
                                                                                                             tCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGGTTTTTTTTTGGTTGGGGGGATAAAAACGATAGATACCTACATTTTAGAGCCTTAGTCCCCCGCGCCAAAAAATTAAAAAGAAATCAAATTGATGCAGGAGCAAACGAGCACCCATgg                                      >  1:427400/1‑151 (MQ=255)
                                                                                                              cGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGt                                     <  2:605003/151‑1 (MQ=255)
                                                                                                              cGTTGCGGCACTGGCGGTTCCTTCCCTCCGTGGTTTTTTTTTGGTTGGGGGGCTAAAACCGGCAGAAAACTACATTGGAGCGGAATAATCCCCCGCGCAAAAAAATAATAAGGACACTAAAAGGAATCAGGaaaaaccgcgcaaac                                          >  1:289337/1‑132 (MQ=255)
                                                                                                                 tGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGttt                                   <  1:554773/150‑1 (MQ=255)
                                                                                                                 tGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGttt                                   <  2:331682/150‑1 (MQ=255)
                                                                                                                       aCTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGt                            <  2:705894/151‑1 (MQ=255)
                                                                                                                       aCTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGt                            <  2:81985/151‑1 (MQ=255)
                                                                                                                         tGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTc                           >  2:569550/1‑150 (MQ=255)
                                                                                                                            cGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATcc                       <  2:7368/151‑1 (MQ=255)
                                                                                                                             ggTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCt                      >  2:943612/1‑151 (MQ=255)
                                                                                                                               ttCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTgc                    >  2:400081/1‑151 (MQ=255)
                                                                                                                                  tctcGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCt                 >  1:531894/1‑151 (MQ=255)
                                                                                                                                     cgctcgGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTAc              <  2:513573/151‑1 (MQ=255)
                                                                                                                                      gctcgGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACg             >  2:910218/1‑151 (MQ=255)
                                                                                                                                         cgGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGc            >  1:181195/1‑149 (MQ=255)
                                                                                                                                            cGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGccc       <  1:891800/151‑1 (MQ=255)
                                                                                                                                                  ttttGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATc    <  1:73515/150‑1 (MQ=255)
                                                                                                                                                    ttgttgGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCaa  <  1:827213/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
TCCGATGGAAAAAACAGAAAAAGATCCGGTCAGTGGTGTATGGACAGTAAGGAACTCAGGGAAATGATTAAATCATGGTGGGCAGAAAAATCCACATCGGAAAAACAGATCGTTGCGGCACTGGCGGTTCTCTCGCTCGGCGTTTTTTGTTGGCTTGGGGTGATAAAACCGATAGATACCTACATTGCAGAGCATCAGTCCCACGCGCAAAAAATTAAGAAGGACATCAAATGGATGCAGGATCAAGCGAGCACCCATGGTTTACTCGGTCATCCTGCGCTTACGCAGCCCATCAA  >  NC_000913/3465019‑3465314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: