Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,200	0	G	A	44.8%	75.9 / 34.3	67	S217F (TCC→TTC)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base G (30/7);  new base A (0/30);  total (30/37)
Fisher's exact test for biased strand distribution p-value = 1.06e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTT  >  NC_000913/3556051‑3556346                                                                                                                                                         |                                                                                                                                                   ccAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGa                                                                                                                                                   <  1:603096/151‑1 (MQ=255)   aaCTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCCCTTTCGACTTCAAGCGAGACGGTATTACCC‑TGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGaaa                                                                                                                                                 <  2:102525/151‑1 (MQ=255)      tgtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAAc                                                                                                                                              >  1:218455/1‑151 (MQ=255)       gtgCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGaaaaa                                                                                                                                               <  2:459070/149‑1 (MQ=255)         gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcc                                                                                                                                           <  2:131783/151‑1 (MQ=255)           cGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcccc                                                                                                                                         <  1:828272/151‑1 (MQ=255)           cGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTcccc                                                                                                                                         >  2:400060/1‑151 (MQ=255)            gCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCg                                                                                                                                        >  2:664566/1‑151 (MQ=255)               accaccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGcc                                                                                                                                      >  1:304267/1‑150 (MQ=255)                 caccTCGGCCCTGTCGCGCTTGTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCCCTTTCAACTTCAAGCGAGACGGTATTCCCC‑GGCCCC‑TGGTCCCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAAACCCCCCGCCa                                                                                                                                     <  1:148441/149‑1 (MQ=255)                      cGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGc                                                                                                                              >  1:930207/1‑151 (MQ=255)                       ggCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCg                                                                                                                             >  2:532503/1‑151 (MQ=255)                            tGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGAttt                                                                                                                         >  1:142359/1‑150 (MQ=255)                             gACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTc                                                                                                                        >  2:575024/1‑150 (MQ=255)                                  gcTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTc                                                                                                                  >  2:269086/1‑151 (MQ=255)                                    tttcccccGACAAAAAAAAAGCGTTG‑GGTGATTTTTCCACTTTCGACTAAAAGGGAGACGTTATCCCCC‑GCCCCC‑GGGTCGCGCGGCGGTTTATAAATTTTCTGTTAAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGc                                                                                                                    <  1:47282/141‑1 (MQ=255)                                        cgccGAGGAAAAAAAAGCGTTG‑GTTTATATTTTCGCTTCCTAATCCAGCCGAGGCGATTTTCCCG‑GGCCCC‑TGGCCGCGCGGCAGTTTAGAAATTTTCTTTCCAGGCAGGAAAAAGCCCCCCGCCAGTGTAGCGATTTCACGCTCAg                                                                                                                <  2:423339/145‑1 (MQ=255)                                           cgacgaCAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                         >  2:556425/1‑151 (MQ=255)                                           cgaaccaaaaaaaAGGTTCGGGTAATTTTTCCTCTTTCGGCTCAAAGCGAGAGGTTATTCCCC‑GCCCCC‑TGGGCGCGCGGAGGGTAAAAAATATTTTTTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCa                                                                                                              <  1:19174/140‑1 (MQ=255)                                              cgacAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                      >  2:748676/1‑151 (MQ=255)                                                aaaaaaaaGCTTTG‑GGTGATTTTTTCCCTTTCGCCTCAAAGCGAGACGGTTTTCCCC‑GGCCCC‑TGGTCGCGCGGCAGTTTATAAATTTTCTTTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                      <  2:942483/147‑1 (MQ=255)                                                  aaaaagGCGTTC‑GGTGATTTTTTCCCTTCCGCCTCAAAGGGAGCCGTTATCCCCC‑GCCCCT‑GGGTCGCGGGGCAGTTTAGGAATATTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGc                                                                                                  <  1:780102/151‑1 (MQ=255)                                                  aaaaaaGCGTCG‑GGTAATTTTTTCTCTTCCGCCTCAAAGCG‑GACGGTTTTACCCGGGCCCC‑GGGCCGCGCGGCAGTTTATAATTTTTCTGTTCAGGAGGGAAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                   <  2:427841/150‑1 (MQ=255)                                                      aaGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCacac                                                                                              >  1:615541/1‑151 (MQ=255)                                                         cGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                             >  1:459886/1‑149 (MQ=255)                                                           ttC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCt                                                                                         >  1:679539/1‑151 (MQ=255)                                                             c‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                        >  2:937335/1‑150 (MQ=255)                                                               ggtattatTTTCCCTTCCGACTTCAAGGGAGCCGGTATTCCCC‑GCCCCC‑GGGCCGCGCGGAGGTTTATAAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTa                                                                                        <  1:171914/144‑1 (MQ=255)                                                               ggTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                       >  1:793355/1‑150 (MQ=255)                                                                 tGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                      >  1:798728/1‑149 (MQ=255)                                                                 tGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                      >  1:603168/1‑149 (MQ=255)                                                                 tGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                      >  1:288703/1‑149 (MQ=255)                                                                 tGATATTTTCACTTTCGACTTCAAGCGAGACGGGATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACGCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                       >  1:66447/1‑148 (MQ=255)                                                                        ttCCCTTCCGACTTAAAGCGAGACGGTTTTCCCC‑GCCCCC‑TGGTCGCGGGGCAGTTTATAAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                              <  2:599475/150‑1 (MQ=255)                                                                        ttCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAAAATGGCGCGGCACACCAGCTAATAGAACTtct                                                                             >  2:354067/1‑151 (MQ=255)                                                                        tccccTTTCGCCTACAAGCGGGACGGTATTCCCC‑GGCCCC‑TGGCCCCGCGGCAGGTTAGAAATTTTTTTTCCAGGAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTtc                                                                              <  1:308903/146‑1 (MQ=255)                                                                           ccTTTCGCCTTCAAGCGAGCCGGTATTCCCC‑GGCCCC‑GGGCCGCGCGGCGGTTTATAAATTTTCTGTTAAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTcc                                                                           <  1:556569/149‑1 (MQ=255)                                                                                cGACTAAAAGCGAGCCGGTATCCCCC‑GGCCCC‑TGGCCGCGCGGCGGTTTATAAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa                                                                      <  1:286176/150‑1 (MQ=255)                                                                                   cTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTg                                                                  >  1:466838/1‑151 (MQ=255)                                                                                        aGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcaca                                                              >  2:637742/1‑150 (MQ=255)                                                                                            agaCGGTATTCCCC‑GCCCCC‑TGGTCGCGCGGCAGGTTATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATg                                                           <  1:645194/149‑1 (MQ=255)                                                                                             gaCGGTATTCCCC‑GGCCCC‑TGGCCGCGCGGCAGTTTATAAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGtt                                                         <  2:642023/150‑1 (MQ=255)                                                                                              aCGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTcc                                                       >  2:195466/1‑151 (MQ=255)                                                                                                gttATTCCCC‑GCCCCC‑GGGTCGCGCGGCAGTTTATAAATTTTCTTTTCATGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                     <  1:631491/149‑1 (MQ=255)                                                                                                 gtttttccc‑GGCCCCGGGGCCGCGCGGCAGTTTAGGAATTTTCTGTTCTGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTccc                                                      <  1:556827/144‑1 (MQ=255)                                                                                                   aTTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCgcgc                                                  >  1:929752/1‑151 (MQ=255)                                                                                                   aTTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCgcgc                                                  >  2:839005/1‑151 (MQ=255)                                                                                                    ttcccc‑GCCCCC‑TGGTCGCGGGGAAGGTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                     <  2:284627/144‑1 (MQ=255)                                                                                                     acccc‑GGCCCC‑TGGTCGCGGGGCAGGTTATGATTATTCTGTTCATGGAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                                <  2:302905/149‑1 (MQ=255)                                                                                                       ccc‑GGCCCG‑TGGCCGCGCGGCAGGTTATAAATTTTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCt                                                 <  1:642944/148‑1 (MQ=255)                                                                                                           ggCCCC‑TGGTCGGGGGGCAGTTTATAAATTTTTTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCa                                            <  1:748667/150‑1 (MQ=255)                                                                                                             cccc‑TGGTCGCGCGGCAGGTTATAAATTTTTTGTCCATGAAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCaa                                           <  2:742276/149‑1 (MQ=255)                                                                                                              ccc‑TGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGtt                                        <  2:85285/151‑1 (MQ=255)                                                                                                              ccc‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGtt                                        <  1:269750/151‑1 (MQ=255)                                                                                                                      cgcgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAgg                                  >  1:514537/1‑150 (MQ=255)                                                                                                                       gcgcgGCAGGTTATAAATTTTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGc                                      <  2:605780/145‑1 (MQ=255)                                                                                                                         gcgGCAGTTTATGAATTTTTTGTTAAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtgt                               <  1:104885/150‑1 (MQ=255)                                                                                                                           ggcggtttATAAATTTTTTTTTCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtg                                <  2:73573/141‑1 (MQ=255)                                                                                                                           ggCAGTTTATAAATTTTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                              <  1:698388/149‑1 (MQ=255)                                                                                                                                 ttagaaaTATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                       <  1:28110/145‑1 (MQ=255)                                                                                                                                 ttATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg                      >  1:761562/1‑151 (MQ=255)                                                                                                                                 ttATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg                      >  1:300408/1‑151 (MQ=255)                                                                                                                                 taagaaaTATTCTGTTCATGCAGGAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGtt                              <  2:898305/138‑1 (MQ=255)                                                                                                                                        ttttctttgcagccAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                   <  2:729448/134‑1 (MQ=255)                                                                                                                                           tCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg             <  2:914303/150‑1 (MQ=255)                                                                                                                                             ttttCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCg             <  1:700462/146‑1 (MQ=255)                                                                                                                                                     cAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGtt  >  2:460681/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                   CCAACTGTGCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTC‑GGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCC‑GGCCCC‑TGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTT  >  NC_000913/3556051‑3556346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑