Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	941,713	0	T	G	52.7%	58.6 / 89.4	55	V252G (GTG→GGG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (3/23);  new base G (29/0);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.39e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-08

TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941568‑941862                                                                                                                                                  |                                                                                                                                                        tactCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                                    >  2:753136/1‑151 (MQ=255)  actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACt                                                                                                                                                     <  1:442187/149‑1 (MQ=255)  actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACt                                                                                                                                                     <  1:655502/149‑1 (MQ=255)  actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGAca                                                                                                                                                     >  2:814985/1‑148 (MQ=255)  actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTAGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCtt                                                                                                                                                    >  2:926101/1‑150 (MQ=255)         cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGAGTTACTATCtt                                                                                                                                            >  2:139972/1‑151 (MQ=255)             cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAAc                                                                                                                                        <  2:775833/151‑1 (MQ=255)             cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAAc                                                                                                                                        <  2:174083/151‑1 (MQ=255)                   gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCa                                                                                                                                   <  2:943809/150‑1 (MQ=255)                    cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGGCGGATAGCGAAAACAGTAAGCTGGTAGTGCAGTTTGGTAATAACCCTGGCGAAACGCGTATGAGTGGCGGTGGGGTGACTTACTATCTTGAAGAGGCAc                                                                                                                                  <  1:852391/150‑1 (MQ=255)                     ggAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACGTTCTATCCTTAAAAGGc                                                                                                                                    >  2:455158/1‑147 (MQ=255)                        aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAg                                                                                                                             <  1:190428/151‑1 (MQ=255)                        aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTATATTTTGAACAAGCAAGcc                                                                                                                               >  2:694139/1‑149 (MQ=255)                        aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTCTTTCTTGAAAAGGgccgcc                                                                                                                               >  1:396397/1‑149 (MQ=255)                              gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCt                                                                                                                       <  2:275511/151‑1 (MQ=255)                              gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTACTATCTTGAAAAAGCACACCCGaa                                                                                                                           >  2:591508/1‑147 (MQ=255)                               aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTa                                                                                                                      <  1:531960/151‑1 (MQ=255)                               aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGATTAATATCTTTAAAAAGCCCGCCAGgaa                                                                                                                          >  1:523654/1‑147 (MQ=255)                                   ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACTATTTTTAAAAAGCACGCCCGAAAACTaa                                                                                                                     >  1:762266/1‑148 (MQ=255)                                     aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAGGGACGCCCGAAAACTAAAGccc                                                                                                                >  2:723999/1‑151 (MQ=255)                                      ccTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTTTATCTTTAAAAGACACGCCACAAAAATAATGccc                                                                                                                >  1:88335/1‑150 (MQ=255)                                          cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATg                                                                                                           <  2:779800/151‑1 (MQ=255)                                          cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGccc                                                                                                                >  2:845871/1‑146 (MQ=255)                                            gcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGat                                                                                                         <  2:776512/151‑1 (MQ=255)                                                       gATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTTCTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCA‑TCg                                                                                                >  1:926351/1‑148 (MQ=255)                                                        aTGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGATTACTATTTTGAAAAAGCACGCCCGAAATCTAATGCCCCCATAATCATCATTCGATc                                                                                             >  2:479151/1‑151 (MQ=255)                                                           gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCcgcg                                                                                         <  2:183287/151‑1 (MQ=255)                                                                aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTAATAACTTTTACAGGCACGGCAGAAAACTAATAGCCGCATGATCATCA‑TCGAttc                                                                                            >  1:86579/1‑141 (MQ=255)                                                                 gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGAGGTACTATCTTTAAAAGGGGCGCCAGAAATCTAAAGCCCGCATGAGCATCA‑TCGATcaccgc                                                                                        >  1:797201/1‑141 (MQ=255)                                                                    cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAAGCACGCCAGAAAACTAATGCCCCCCTGATCATCC‑TCGATCCCCGCtataac                                                                                  >  2:127443/1‑147 (MQ=255)                                                                        ggATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CAcc                                                                           <  1:420347/151‑1 (MQ=255)                                                                              cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGcc                                                                     <  2:276289/151‑1 (MQ=255)                                                                                aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGACCATCA‑TCCATCCCCGCTATACCGA‑CACCCGGGcc                                                                     >  1:565151/1‑149 (MQ=255)                                                                                aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAAGCACCCCAGAAAAATAAAGGCCGCATGATCCTTA‑TCCATCCGCGCCATACCCA‑CACCGGTGCCgg                                                                   >  1:615463/1‑151 (MQ=255)                                                                                aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGAGGGGGGGCTTTATCTCTTTTACAGGGACCCCCGAAATCTAATGCCCGCATGATCATCC‑TCCATCCGCGGGATACCCACCACCGGGGcgg                                                                    >  1:682759/1‑149 (MQ=255)                                                                                     aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGGGCCGGGcgcg                                                              >  2:895281/1‑151 (MQ=255)                                                                                           cTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGGGCCGGGCGCGgaga                                                          >  2:378789/1‑149 (MQ=255)                                                                                           cTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGAGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGT‑CACCGGTGCCGGGCGCGAAGAt                                                         <  1:328436/150‑1 (MQ=255)                                                                                            tGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCA‑TCGATCCCCCCTATAACGA‑CACCGGGGCCGGGGGCGAAGa                                                          >  2:247720/1‑148 (MQ=255)                                                                                            tGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAATTAATCTCTTGAAAAAGCACGCCCGAAAACTAATGCCCCCACGATCATCA‑TCGGTCCGCGCGATACCCC‑CACCGGAGCCCGGGGCGAAGa                                                          >  2:461643/1‑148 (MQ=255)                                                                                                aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATAACGA‑CACCCGGGCCGGGGGCGGAGAagag                                                      >  1:524649/1‑148 (MQ=255)                                                                                                      gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACGAATTTTAAGAGGCCCGCCAGAAATACAATTTCCCCAAGATCATCA‑ACGATCCCCGCCCTACCGA‑CACCGGTGCCGGGCGGGAAGATGAGTGGGTccc                                              >  2:593402/1‑150 (MQ=255)                                                                                                           gTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCg                                        <  2:440299/151‑1 (MQ=255)                                                                                                                aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTTTATCTTTAACAAGCACCCCAGAAATCTAATGCCCGCCTGATCATCA‑TCGATCCGCGCTATACCCA‑CACCCGTGCCCGGCGCGAAGAAGAGGGGAACCCTATTCGTcccg                                   >  1:555224/1‑149 (MQ=255)                                                                                                                  cccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGGGCCGGGCGCGAAGATGAGTGGGTCCCTTTTTGTCCcgg                                  >  1:502385/1‑150 (MQ=255)                                                                                                                   ccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCCTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATAACGA‑CACCGGGGGCGGGGGCGAAGATGAGGGGGTCCCTATTTGTCCggg                                  >  1:833944/1‑149 (MQ=255)                                                                                                                      ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAAc                               <  2:396400/149‑1 (MQ=255)                                                                                                                      ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACa                              <  1:751100/150‑1 (MQ=255)                                                                                                                           aaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGcc                        <  2:558513/151‑1 (MQ=255)                                                                                                                            aCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCg                       <  2:705522/151‑1 (MQ=255)                                                                                                                                 aaTGAGTGGCGGTGGGGGGACTTTCTATCTTTAAAAGGCACGCCAGAAAATTAATGGCCCCATGATCATCA‑TCGATCCGCGCTATAACGA‑CACCGGTGCCGGGCGCGGAGATGAGTGGATCCCCATTCCTCCCGGAACAGATGCCGCAc                    >  1:512008/1‑149 (MQ=255)                                                                                                                                        ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc            <  1:926678/150‑1 (MQ=255)                                                                                                                                        ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc            <  1:114244/150‑1 (MQ=255)                                                                                                                                             tGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTg      >  1:537673/1‑151 (MQ=255)                                                                                                                                                 gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  <  2:845419/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                        TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCA‑TCGATCCGCGCTATACCGA‑CACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941568‑941862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑