Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG52.7% 58.6 / 89.4 55V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (3/23);  new base G (29/0);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.39e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-08

TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941568‑941862
                                                                                                                                                 |                                                                                                                                                       
tactCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                                    >  2:753136/1‑151 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACt                                                                                                                                                     <  1:442187/149‑1 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACt                                                                                                                                                     <  1:655502/149‑1 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGAca                                                                                                                                                     >  2:814985/1‑148 (MQ=255)
 actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTAGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCtt                                                                                                                                                    >  2:926101/1‑150 (MQ=255)
        cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGAGTTACTATCtt                                                                                                                                            >  2:139972/1‑151 (MQ=255)
            cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAAc                                                                                                                                        <  2:775833/151‑1 (MQ=255)
            cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAAc                                                                                                                                        <  2:174083/151‑1 (MQ=255)
                  gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCa                                                                                                                                   <  2:943809/150‑1 (MQ=255)
                   cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGGCGGATAGCGAAAACAGTAAGCTGGTAGTGCAGTTTGGTAATAACCCTGGCGAAACGCGTATGAGTGGCGGTGGGGTGACTTACTATCTTGAAGAGGCAc                                                                                                                                  <  1:852391/150‑1 (MQ=255)
                    ggAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACGTTCTATCCTTAAAAGGc                                                                                                                                    >  2:455158/1‑147 (MQ=255)
                       aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAg                                                                                                                             <  1:190428/151‑1 (MQ=255)
                       aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTATATTTTGAACAAGCAAGcc                                                                                                                               >  2:694139/1‑149 (MQ=255)
                       aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTCTTTCTTGAAAAGGgccgcc                                                                                                                               >  1:396397/1‑149 (MQ=255)
                             gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCt                                                                                                                       <  2:275511/151‑1 (MQ=255)
                             gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTACTATCTTGAAAAAGCACACCCGaa                                                                                                                           >  2:591508/1‑147 (MQ=255)
                              aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTa                                                                                                                      <  1:531960/151‑1 (MQ=255)
                              aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGATTAATATCTTTAAAAAGCCCGCCAGgaa                                                                                                                          >  1:523654/1‑147 (MQ=255)
                                  ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACTATTTTTAAAAAGCACGCCCGAAAACTaa                                                                                                                     >  1:762266/1‑148 (MQ=255)
                                    aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAGGGACGCCCGAAAACTAAAGccc                                                                                                                >  2:723999/1‑151 (MQ=255)
                                     ccTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTTTATCTTTAAAAGACACGCCACAAAAATAATGccc                                                                                                                >  1:88335/1‑150 (MQ=255)
                                         cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATg                                                                                                           <  2:779800/151‑1 (MQ=255)
                                         cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGccc                                                                                                                >  2:845871/1‑146 (MQ=255)
                                           gcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGat                                                                                                         <  2:776512/151‑1 (MQ=255)
                                                      gATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTTCTATCTTTAAAAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCg                                                                                                >  1:926351/1‑148 (MQ=255)
                                                       aTGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGATTACTATTTTGAAAAAGCACGCCCGAAATCTAATGCCCCCATAATCATCATTCGATc                                                                                             >  2:479151/1‑151 (MQ=255)
                                                          gCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcgcg                                                                                         <  2:183287/151‑1 (MQ=255)
                                                               aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTAATAACTTTTACAGGCACGGCAGAAAACTAATAGCCGCATGATCATCATCGAttc                                                                                            >  1:86579/1‑141 (MQ=255)
                                                                gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGAGGTACTATCTTTAAAAGGGGCGCCAGAAATCTAAAGCCCGCATGAGCATCATCGATcaccgc                                                                                        >  1:797201/1‑141 (MQ=255)
                                                                   cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAAGCACGCCAGAAAACTAATGCCCCCCTGATCATCCTCGATCCCCGCtataac                                                                                  >  2:127443/1‑147 (MQ=255)
                                                                       ggATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACAcc                                                                           <  1:420347/151‑1 (MQ=255)
                                                                             cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGcc                                                                     <  2:276289/151‑1 (MQ=255)
                                                                               aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGACCATCATCCATCCCCGCTATACCGACACCCGGGcc                                                                     >  1:565151/1‑149 (MQ=255)
                                                                               aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAAGCACCCCAGAAAAATAAAGGCCGCATGATCCTTATCCATCCGCGCCATACCCACACCGGTGCCgg                                                                   >  1:615463/1‑151 (MQ=255)
                                                                               aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGAGGGGGGGCTTTATCTCTTTTACAGGGACCCCCGAAATCTAATGCCCGCATGATCATCCTCCATCCGCGGGATACCCACCACCGGGGcgg                                                                    >  1:682759/1‑149 (MQ=255)
                                                                                    aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGcgcg                                                              >  2:895281/1‑151 (MQ=255)
                                                                                          cTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGgaga                                                          >  2:378789/1‑149 (MQ=255)
                                                                                          cTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGAGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGTCACCGGTGCCGGGCGCGAAGAt                                                         <  1:328436/150‑1 (MQ=255)
                                                                                           tGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCCCCCTATAACGACACCGGGGCCGGGGGCGAAGa                                                          >  2:247720/1‑148 (MQ=255)
                                                                                           tGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAATTAATCTCTTGAAAAAGCACGCCCGAAAACTAATGCCCCCACGATCATCATCGGTCCGCGCGATACCCCCACCGGAGCCCGGGGCGAAGa                                                          >  2:461643/1‑148 (MQ=255)
                                                                                               aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATAACGACACCCGGGCCGGGGGCGGAGAagag                                                      >  1:524649/1‑148 (MQ=255)
                                                                                                     gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACGAATTTTAAGAGGCCCGCCAGAAATACAATTTCCCCAAGATCATCAACGATCCCCGCCCTACCGACACCGGTGCCGGGCGGGAAGATGAGTGGGTccc                                              >  2:593402/1‑150 (MQ=255)
                                                                                                          gTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCg                                        <  2:440299/151‑1 (MQ=255)
                                                                                                               aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTTTATCTTTAACAAGCACCCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCCACACCCGTGCCCGGCGCGAAGAAGAGGGGAACCCTATTCGTcccg                                   >  1:555224/1‑149 (MQ=255)
                                                                                                                 cccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGTGGGTCCCTTTTTGTCCcgg                                  >  1:502385/1‑150 (MQ=255)
                                                                                                                  ccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCCTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATAACGACACCGGGGGCGGGGGCGAAGATGAGGGGGTCCCTATTTGTCCggg                                  >  1:833944/1‑149 (MQ=255)
                                                                                                                     ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAAc                               <  2:396400/149‑1 (MQ=255)
                                                                                                                     ggCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACa                              <  1:751100/150‑1 (MQ=255)
                                                                                                                          aaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGcc                        <  2:558513/151‑1 (MQ=255)
                                                                                                                           aCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCg                       <  2:705522/151‑1 (MQ=255)
                                                                                                                                aaTGAGTGGCGGTGGGGGGACTTTCTATCTTTAAAAGGCACGCCAGAAAATTAATGGCCCCATGATCATCATCGATCCGCGCTATAACGACACCGGTGCCGGGCGCGGAGATGAGTGGATCCCCATTCCTCCCGGAACAGATGCCGCAc                    >  1:512008/1‑149 (MQ=255)
                                                                                                                                       ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc            <  1:926678/150‑1 (MQ=255)
                                                                                                                                       ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc            <  1:114244/150‑1 (MQ=255)
                                                                                                                                            tGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTg      >  1:537673/1‑151 (MQ=255)
                                                                                                                                                gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  <  2:845419/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                       
TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941568‑941862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: