Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	907,602	0	A	C	47.8%	50.5 / 16.0	47	G227G (GGT→GGG)	ybjT	putative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (18/6);  new base C (0/22);  total (18/29)
Fisher's exact test for biased strand distribution p-value = 5.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGCG  >  NC_000913/907462‑907751                                                                                                                                               |                                                                                                                                                      gTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCg                                                                                                                                             <  1:990850/151‑1 (MQ=255)   atcatcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGcc                                                                                                                                           <  1:286047/151‑1 (MQ=255)     catcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCAt                                                                                                                                         >  2:19628/1‑151 (MQ=255)         cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATaaa                                                                                                                                      >  1:589405/1‑150 (MQ=255)             agcagTTCTTTTTTCAGCCCCGAAATCACCCCCCGGGGGGGGGGGGGCGGTACGAAAGTAATCACATTGAAAAACCACACCAAAACCCGGCGGGGGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGTTTCCCGCTCACCGCCATAAAATGt                                                                                                                                  <  2:389954/150‑1 (MQ=255)             agcagATCGTTTTTCAGCCCTTGAATCACCCCCCTGGGGGGGGGGGGCGGTAGGAAAGTAACCACATTGAAAACCCCCCCCAAAACCCGGGGGGGGGGG‑AGGGGGAGGGGA‑ATCACCCGGGGGCGCTTCCCGCTCACCGCCATAAAATg                                                                                                                                   <  2:720470/149‑1 (MQ=255)               cagATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑CGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCCTAAAATGTTc                                                                                                                                >  2:639908/1‑150 (MQ=255)                   tCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACt                                                                                                                           >  2:232855/1‑151 (MQ=255)                      tttttCACCCCCGGAATCAACCCCCGGGCGGGGGGGGGCGGTAGGAAAGTAATCACTTTGAGAACCCCCACCAAAACCCAGGGGGTGGGG‑GGGGGGATGGGG‑ATAACCCGGCGGCGCTTCCCGCTCCCCGCCATAAAATGTTCAAACTg                                                                                                                          <  1:920043/147‑1 (MQ=255)                         ttCAGCCCCTGAATCAACGCCCTGGCGGGAGTTTGCGGTACGTAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑GGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGc                                                                                                                      >  1:104822/1‑150 (MQ=255)                           cggCCCCTGAATCACCGCCCGGGGGGTGGGGGGGGGTGCGAAAGAAATCACATTGAAAAACCACCCCAAAATCCGGGGGGGGGGG‑GGGGGGAGGGGG‑ACCAACCAGGGGGGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGc                                                                                                                      <  1:52952/146‑1 (MQ=255)                           cacccccTGAATCACCCCCCGGGGGGTGGTGGGGGGTAGGGAAATAATCACATTGAAAAACCCCACCAAACCCCGGGGGGGGGGG‑GGGGGGGGGGGA‑ATCAACAACCGGCTCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                                                                                    <  2:446358/147‑1 (MQ=255)                              ccccTAAATCACCCCCCGGGGGGGGGGGGGGGGTAGGAAAGTAATCACATTGAAAACCCCCCCCAAAACCCAGGGGGGGGGG‑AGGGGGAGGGGA‑ATCACCAGGCGGCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGa                                                                                                                   <  2:684510/148‑1 (MQ=255)                                  tGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                                                                             >  2:340919/1‑150 (MQ=255)                                      caaccccccGGGGGGTGGTGGGCGGTACGAAATAAATCAAAGT‑AAAAACCCCCCCAAAAACCCGGGGGGGGGG‑GGGGGGATGGGGAATCACCCGGCGGCTCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAc                                                                                                        <  2:921947/145‑1 (MQ=255)                                        aaCGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACct                                                                                                      >  2:136878/1‑151 (MQ=255)                                          cGCCCGGGCGGGGGTGGGCGGTGGGGAAGTAACCACATTGAAAAACCACCCCGAAACCGGGGGGGGGGGG‑GGGGGGATGGGG‑ATCAACCGGCGGCCCTTCCCGCCCTCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctct                                                                                                    <  2:867158/151‑1 (MQ=255)                                            cccGGGCGGTGGGGGGCGGTCGGGAAGAAACCCTATGGAAAAACCACACCGAAACCCAGGGGGGGGGG‑AGGGGGTGGGGG‑ATCAACAAGCGGGTCTTCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTg                                                                                                   <  1:781848/150‑1 (MQ=255)                                             ccggggggggggggggggTAGGGAAGAAACCACATGAAAACCCCACCCCAAAACCCGGGGGGGGGGG‑AGGGGGAGGGGG‑ATCACCCAGGGGCGCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACct                                                                                                      <  2:1018306/134‑1 (MQ=255)                                               tGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTcc                                                                                               >  1:9335/1‑151 (MQ=255)                                               ggggggggggggggggTACGAAAGAAACCACATTGAAAACCCCCCCGGAAACCCGGGGGGGGGGG‑GGGGGGAGGGGG‑ATCAACCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctc                                                                                                     <  1:630943/135‑1 (MQ=255)                                                 ggggtgggGGGGGGAAGGAAAGTAAACACATGGAAAACCCACCCAAAAACCCAGGGGGGGGGG‑AGGGGGATGGGA‑ATAACCCGGCGGCGCTTCCCTCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcgg                                                                                             <  2:629809/149‑1 (MQ=255)                                                     tggtggGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGAGCCggcggc                                                                                         >  2:144558/1‑151 (MQ=255)                                                       gggggCGGTACGGAAGTAATCACATTGAAAACCCCCCCCGAAACCCAGGGGGTGGGG‑GGGGGGAGGGGG‑ATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCtt                                                                                       <  1:257672/149‑1 (MQ=255)                                                           gCGGTAGGAAAGAAACCACATTAAAAACCCCCCCCAAAACCCGGGGGGGGGGG‑GGGGGGAGGGGG‑ATAAGCCAGCGCCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGaa                                                                                   <  2:348543/151‑1 (MQ=255)                                                             ggTACGGAAGTAATCACATTAAAAACCCACCCCAAAACCCGGGGGGGGGGG‑GGGGGGATGGGG‑ATCACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAg                                                                                  <  1:560696/150‑1 (MQ=255)                                                                 cGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                                                                             >  1:499304/1‑151 (MQ=255)                                                                     aGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgtgt                                                                         >  2:125548/1‑151 (MQ=255)                                                                        aatcagctggaaaacccACACCAAAACACAGGGGGGGGGG‑GGGTGGTGGGAG‑AACAACCACCGGCTTTCTCCCATCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTc                                                                       <  2:395610/135‑1 (MQ=255)                                                                        aaccaaaTTGAAAACCCACCCCAAAATCCGGGGGGGGGGG‑GGGGGGAGGGGG‑ATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCg                                                                      <  2:589396/145‑1 (MQ=255)                                                                         accacaTGGAAAACCCACCCCAAAACCCAGGGGGGGGGG‑AGGGGGAGGGGG‑ATCACCCAGCGGCCCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTc                                                                       <  2:515738/147‑1 (MQ=255)                                                                            atatgaaaacaCCCCACCAAACACCAGGGGGGGGGG‑GGGGGGGGGGGT‑ATAAACCAGGGGCGCTAACCGCTCTCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTg                                                                   <  1:132412/140‑1 (MQ=255)                                                                                           accaaaaccgggggggggggg‑ggggggAGGGGA‑ATAAACCGGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaa                                                     <  1:381647/136‑1 (MQ=255)                                                                                            ccGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaaca                                                   >  2:699649/1‑150 (MQ=255)                                                                                            ccGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaaca                                                   >  1:841303/1‑150 (MQ=255)                                                                                                aaccccgtgggTGGGGTAGGGGGGGGGGC‑ACCAACCGGCGGGTCTGACCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCGCCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTTGCCGTATGATCTaa                                                     <  2:388408/137‑1 (MQ=255)                                                                                                aaTCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGc                                               >  1:115625/1‑150 (MQ=255)                                                                                                     ggggggggggg‑ggggggggggGA‑ATAACCCGGCGGCTTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCa                                          <  1:122707/147‑1 (MQ=255)                                                                                                      gggggggggg‑ggggGAATGGGG‑ATCAACCGGCGGCGCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAg                                         <  2:691531/148‑1 (MQ=255)                                                                                                         ggTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGAt                                       >  1:1031055/1‑149 (MQ=255)                                                                                                                         gggg‑atCACCCGGCGGCGCTTCCCGCTCGCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGttt                         <  2:884896/148‑1 (MQ=255)                                                                                                                         gggg‑atCACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGttt                         <  1:454409/148‑1 (MQ=255)                                                                                                                                 aaCCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCa                >  1:691404/1‑150 (MQ=255)                                                                                                                                        ggcgCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCg         <  2:518362/150‑1 (MQ=255)                                                                                                                                          ctcTTACCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCgt        <  1:340921/147‑1 (MQ=255)                                                                                                                                             ttACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGc   >  2:153097/1‑151 (MQ=255)                                                                                                                                              tACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGCg  >  2:831276/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                      GTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGG‑AGGGGGATGGGG‑ATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGCG  >  NC_000913/907462‑907751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑