| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,080,837 | 0 | G | T | 42.9% | 62.1 / 26.1 | 49 | intergenic (+24/‑519) | putP/efeU | proline:sodium symporter/ferrous iron permease (pseudogene) |
| Reads supporting (aligned to +/- strand): ref base G (8/20); new base T (21/0); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAA > NC_000913/1080697‑1080984 | gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTca < 2:837084/151‑1 (MQ=255) aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTTCGGCGGTTTTGTTTGGCTTcagc > 1:413321/1‑150 (MQ=255) gTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCg < 1:571643/151‑1 (MQ=255) aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCTGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCggg < 2:701546/151‑1 (MQ=255) tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGCTCCGCACCGGGttt > 2:4743/1‑149 (MQ=255) ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTgc < 2:124523/150‑1 (MQ=255) gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGTTCAGCAGCGGGTTGCGCTc > 1:872104/1‑151 (MQ=255) ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGGTTCAGCAGCGGGGTGCGCcccc > 1:467300/1‑151 (MQ=255) tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCcccc > 1:489819/1‑150 (MQ=255) tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTGTTTTGCTTTTGCAGCGGGGTTTGCTccc > 2:885864/1‑147 (MQ=255) gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTaa < 2:231947/150‑1 (MQ=255) ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGATTTTTTTGGGTTCCGGCACGGGGTGCGGCCCCtt > 1:512819/1‑147 (MQ=255) aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAAtgtg < 2:53686/150‑1 (MQ=255) aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGCTTCAGCAGCGGGTTGCGCTCCCTTTATTTGcc > 1:717505/1‑151 (MQ=255) gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTc < 1:921580/151‑1 (MQ=255) gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCCTCAGCAGCGGGGTGCGCCCCCCTTATGTGCCTc > 2:865704/1‑149 (MQ=255) gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGc > 1:487945/1‑151 (MQ=255) gcCGTCAGCGGCGATGCAAAAACGCCTTGCCGAGGCCGACGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCCAAAGCggg > 2:721360/1‑127 (MQ=255) tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGCTTTTTTTTGCTTACGCAGCGGGGTGCGCTCCCCTAATGTGCCTCGcc > 1:416845/1‑148 (MQ=255) cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAACGGGGTTCGCCCCCCTTAAGTGCCCCGcc > 2:376482/1‑147 (MQ=255) cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTg < 2:685708/151‑1 (MQ=255) gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTTAGCAGCGGGGTGCGCCCCCCTAATGTGCCTCGCCCTATtaaat > 2:124629/1‑147 (MQ=255) gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCCTAATGTGCCCCGCCCTATaaaat > 2:576687/1‑147 (MQ=255) gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGa < 2:584729/151‑1 (MQ=255) aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTAATGGGCCCCGCCATATAAA‑tt > 1:260218/1‑148 (MQ=255) tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCCGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑tttta > 2:915973/1‑147 (MQ=255) aaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTg < 1:243016/151‑1 (MQ=255) aaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGc < 2:998456/150‑1 (MQ=255) aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCCGCAGGGGGTTTCGCTCCCCTTATGTGGCTCGCCCTATAAA‑ATTAAt > 1:878894/1‑144 (MQ=255) gCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAggg < 1:45950/151‑1 (MQ=255) tttGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGGTTTGTTTTGCTTTAACAACGGGGTGCGCCCCCTTAATGAGGCTCGCCCTATAAA‑TTTAATGGGGGAggg > 2:455461/1‑149 (MQ=255) tGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAgc < 1:759214/150‑1 (MQ=255) gCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAgcgc < 1:1029244/151‑1 (MQ=255) gCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAgcgc < 1:463921/151‑1 (MQ=255) ccGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAgcgcg < 2:1027822/151‑1 (MQ=255) aGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAg < 1:246170/151‑1 (MQ=255) ccGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCCGCAGCGGGGTTCGCTCCCTTAAATTGCCTCGCCCTATAAA‑TTGAATGGTGGCGGGAGCGGGCAggg > 2:32976/1‑150 (MQ=255) ccGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGTTGCGCTCCCTTTATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAgggg > 1:931132/1‑151 (MQ=255) cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCCTATAAA‑TTGAATGGGGCAGGGGGCGCGCAggggg > 2:468123/1‑151 (MQ=255) tGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAACGGGGTGGGCCCCCCTAATGAGGCTCCCCCTATAAATTTAAAGGGTGC‑GGGAGCGCGCCgggggggg > 2:287298/1‑151 (MQ=255) gcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCa < 2:348910/151‑1 (MQ=255) cTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTTGCTTCAGCAGCGGGGTGCGCCCCCCTTATGTGCCTCGCCCTATAAA‑ATGAAATGGGCAGGGGGCGCGCAGGGGGCGGCCaa > 1:529701/1‑149 (MQ=255) tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCCTATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATc > 1:742173/1‑150 (MQ=255) tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGCTTCAGCAGCGGGGTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTTAATGGGGCAGGGAGCGCGCAGGGGGCGGCCAATcg > 1:841045/1‑151 (MQ=255) tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTTGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCCCCCCCTATTAA‑TTGAATGGTGCCGGGGGGGCGCAggggg > 2:951710/1‑141 (MQ=255) cGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCg < 1:1017057/151‑1 (MQ=255) gCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCt < 2:705176/150‑1 (MQ=255) aGCTAAGGGACTTAGCCTGCGGCGGTTTTTTTTGGGTTCAGCAGCGGGGTGTGGTCCCCTAATGGGGCTCGCCATATAAA‑TTTAATGGTGCAGGGGGGGCGCAGGGGGGGGCCCATCCGCCCCGCCCCCCGCTGTCCCCGGCCTCgggg > 2:1015325/1‑149 (MQ=255) ttttGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc > 1:443020/1‑150 (MQ=255) tgtttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa > 1:1012949/1‑149 (MQ=255) | GGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGTTTTGTTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAA‑TTGAATGGTGCAGGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCTGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAA > NC_000913/1080697‑1080984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |