| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,890 | 0 | C | A | 41.7% | 48.7 / 15.5 | 36 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (17/4); new base A (0/15); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTC > NC_000913/357743‑358033 | aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaacaa < 2:494478/150‑1 (MQ=255) cGTAGCGCATCAGACAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccg < 2:258556/150‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgc < 1:283352/150‑1 (MQ=255) tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg > 1:633252/1‑151 (MQ=255) cgcccGCAGCCCTGTGTGGGC‑GCTTGCGGGATTTCATTCCCCCAACCACCCGCCCTTTTTCCCACCG‑CCTTGTAAAAAAATGCTTTCGGGGCGGTTGTCGGCCACTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑g < 1:713337/148‑1 (MQ=255) gCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑ggg > 1:946137/1‑151 (MQ=255) cGCAGACTTGTTCCAGCGCCTACCGTGT‑TTCATTCCCCCAAACACCCGCGCTTTTCCCCACCG‑CCGCGCGAAAAATTGCCCCCGCGGGGGCTGTCTGCCAGCTATTTTTCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑g < 2:80725/147‑1 (MQ=255) cAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gggg > 2:344979/1‑148 (MQ=255) gtgtCAGCGCCAACGGGGT‑TTAATTCCCCCCAACACCCGCGATTTTACCCACCC‑CCATGTCAAAAAATCCTTCCGCGGCGGCTTTCGGCCAGCTTTTTTCCCGCCGAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑aa < 1:398476/149‑1 (MQ=255) ttccggcgcttcccGTGT‑TTCATCCCCGCAACCACCCGCGCTTTCCCCCACCG‑CCATGTGAAAAAAGCCCTGCGGGGCGGTTGTCTGCCAGTTATTTTCCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAgag‑a < 2:107420/135‑1 (MQ=255) ccAGCGTCTCCCGTGA‑TTCTTTCCCGCAAACACCCCCGCTTTTACCCACCC‑CCAGGGAAAAAAATGCCTGCGGGGGGTTTGTCTGCCGGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGc < 2:677918/150‑1 (MQ=255) cggCGGCTACCGTTT‑TTCATTCCCCCCAACACCCGCCTTTTTACCCACCC‑CCATGTCAAAAAACGCCTCCGCGGCGGCTGTCGGCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAg < 2:313211/147‑1 (MQ=255) cAGCGGCTCCCGTGT‑TTCATTCCCCCCAACACCCGCGCATTTACCCAACG‑CTATGTCAAAAAATGCCTCCGCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑aaa < 1:639819/146‑1 (MQ=255) ccgccgcctccctttttCCTTTCCCCCCACCACCCCCCCTTTTCCCAACCCCCATGTGAAAAAATCCCTCCGGGGCGGTTGTCGGCCGGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGc < 2:668496/136‑1 (MQ=255) gcctcccgtatttCTTTCCCCCCACCAACCGCCCATTACCCCAACC‑CCATGTAAAAAAACCCTTCCGCGGGGGCTGTCGGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCttt < 2:365774/138‑1 (MQ=255) gCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑aaaaaaattt > 1:14579/1‑141 (MQ=255) cTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGgaaag‑aaaaaaaatt > 1:873355/1‑140 (MQ=25) tACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑gaaaaaatttttga > 1:723870/1‑139 (MQ=25) tACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑aaaaaaattt > 1:161260/1‑139 (MQ=255) cccGTTA‑TTCTTTCCCCCCAACACCCGCGTTTTTTCCCACCG‑CCATGTACAAAAATGCCTCCGCGGCGGTTTTCGGCCACTTTTTTTCCCGCGAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGccc < 2:797092/150‑1 (MQ=255) gTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGGAG‑AAAAACTTTTAAAcc > 1:893819/1‑148 (MQ=25) cATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑gaaaaaacttt > 2:379016/1‑129 (MQ=25) ttccccccAACACCCCCGCTTTTACCCACCC‑CCATGTAAAAAATTCCCTCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGt < 2:895394/136‑1 (MQ=255) ccGCCAACACCCGCGTATTTACCCACCG‑CCATGTGAAAAAATGCTTTCGCGGCGGCTGTCTCCCAGCTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTgg < 1:163057/151‑1 (MQ=255) aacCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAG‑AAAAAAATTTTAGCCCCTCCTGTTGTGTggggatagc > 1:235069/1‑143 (MQ=25) ccGCGCTTTTACCCACCG‑CCTTGTAAAAAAAGGCTTCCGCGGCGCCTGTCTGCAAGTTTTTTTCCCGCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgc < 2:763021/151‑1 (MQ=255) ccGCGCTTTTACCCACCC‑CCTTGTCAAAAAATGCCTCCGCGGCGGTTGTCGCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 1:941688/148‑1 (MQ=255) tATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAGAAAAAAAATTTAAACCC‑CCCTGTTGTGTGGGGATCGCTGCTggggaca > 1:12474/1‑145 (MQ=25) aTGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTTCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGCAGAG‑AAAAAAATTTTAAACCCCCCTTTTGTGTGGGAAACGCCGCGGGTGAA‑AAAATGGGAAggg > 1:422698/1‑151 (MQ=255) gtgAAAAAATGCCTCCGGGGCGGCTGTCTGCCAGTTATTTTCCCCCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAggg < 1:26382/149‑1 (MQ=255) tgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAAAG‑AAAAAAAATTAAATACGTCCTGTTGTGTggggctcgccgccg > 2:135974/1‑118 (MQ=255) gCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAGAAAAAAAATTTAAACCC‑CCATGTTGTGTGGGGAACGCTGCTGGGGAC‑AAAATGGGAAGGGGAgggggggg > 1:143206/1‑142 (MQ=255) ccTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGGG‑AAAAAAATTTTAACCCCCCCTTTTTTTGGGGAATCGCTGGTGGGGAC‑AAAATGGGAAGGGAAGGggggcg > 2:546068/1‑148 (MQ=255) cgcgGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCGAGAG‑AAAAAACTTTAAAACCGTCCTGTTTTGTGGGGATTGCTGCGGGGGAC‑AAAATGGGAAGGGaggggggggg > 2:519447/1‑135 (MQ=255) acttttttcCCGCCGCAACAAAGCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa < 2:150726/144‑1 (MQ=255) tcccgccgCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt < 1:165736/147‑1 (MQ=255) gccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGCAAAG‑AAAAAAATTTTAAACCCCCCTGTTTTGTGGGAATCCCCGCTGGGGACAAATTTGGAAGGGGAAG‑GGGGGGGATCAAATCCAGAACCACGCCAAATTAGTTTGcccc > 1:269725/1‑149 (MQ=255) | ATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCCAACAACCGCGCATTTATCCAACG‑CCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC‑AATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTC > NC_000913/357743‑358033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |