Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,358	C→T	P372S (CCT→TCT)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,358	0	C	T	100.0%	207.3 / NA	54	P372S (CCT→TCT)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/30);  total (24/30)

CTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGG  >  NC_000913/4182212‑4182497                                                                                                                                                   |                                                                                                                                            cTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTgg                                                                                                                                         >  1:220702/1‑151 (MQ=255)       tGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAgcc                                                                                                                                   >  2:1037520/1‑151 (MQ=255)           ccAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGccgccg                                                                                                                               >  1:506721/1‑151 (MQ=255)                cTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCg                                                                                                                          >  2:347563/1‑151 (MQ=255)                     cacaAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGaa                                                                                                                      <  2:807315/150‑1 (MQ=255)                        aaGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAagc                                                                                                                    >  2:513951/1‑149 (MQ=255)                           cGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTg                                                                                                               <  2:358919/151‑1 (MQ=255)                              aTCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGaaa                                                                                                            <  2:637596/151‑1 (MQ=255)                                  aaaCGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCt                                                                                                        <  1:516723/151‑1 (MQ=255)                                     cGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGtt                                                                                                     <  2:914095/151‑1 (MQ=255)                                         gTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCga                                                                                                  <  2:529263/150‑1 (MQ=255)                                           tCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCgaga                                                                                                <  1:398533/150‑1 (MQ=255)                                           tCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCgaga                                                                                                <  2:128602/150‑1 (MQ=255)                                            cACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAAc                                                                                              <  2:19326/151‑1 (MQ=255)                                                  cGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGttc                                                                                        >  1:550634/1‑151 (MQ=255)                                                   gATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGttct                                                                                       >  2:880844/1‑151 (MQ=255)                                                    aTCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGttctt                                                                                      >  2:205631/1‑151 (MQ=255)                                                     tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGttcttc                                                                                     >  2:460907/1‑151 (MQ=255)                                                               ggCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGAcc                                                                           <  2:699905/151‑1 (MQ=255)                                                                 cccATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGt                                                                         <  2:731256/151‑1 (MQ=255)                                                                    atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTAt                                                                      <  1:862641/151‑1 (MQ=255)                                                                       tatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGAc                                                                   >  2:481070/1‑151 (MQ=255)                                                                            ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAGGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTc                                                              >  2:198821/1‑151 (MQ=255)                                                                                aaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCg                                                          >  2:1030365/1‑151 (MQ=255)                                                                                   cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGtt                                                       >  2:236770/1‑151 (MQ=255)                                                                                     tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGtt                                                       <  2:506715/149‑1 (MQ=255)                                                                                      aCGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGt                                                    <  2:866794/151‑1 (MQ=255)                                                                                       cGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATAACTTGTCTGCGGTTGGTc                                                   >  2:422043/1‑151 (MQ=255)                                                                                        gtgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCg                                                  <  1:472294/151‑1 (MQ=255)                                                                                        gtgtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCg                                                  >  2:405534/1‑151 (MQ=255)                                                                                          gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGt                                                 <  2:606626/150‑1 (MQ=255)                                                                                            cGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATg                                              >  2:391287/1‑151 (MQ=255)                                                                                             gACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATg                                              >  2:581410/1‑150 (MQ=255)                                                                                                ccAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGt                                          <  1:429516/151‑1 (MQ=255)                                                                                                   aCTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCa                                       <  1:739743/151‑1 (MQ=255)                                                                                                      aaCGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAAc                                     <  2:560243/150‑1 (MQ=255)                                                                                                        cGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGt                                  <  1:743913/151‑1 (MQ=255)                                                                                                           ccGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTtct                               <  1:513957/151‑1 (MQ=255)                                                                                                           ccGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTtc                                <  2:573288/150‑1 (MQ=255)                                                                                                           ccGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTtc                                <  2:587883/150‑1 (MQ=255)                                                                                                             gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTctct                             <  2:117273/151‑1 (MQ=255)                                                                                                             gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTctct                             <  2:802625/151‑1 (MQ=255)                                                                                                             gTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTctct                             >  2:769631/1‑151 (MQ=255)                                                                                                                 gAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTctgctg                         >  1:918105/1‑151 (MQ=255)                                                                                                                 gAGCGCACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTctgc                           <  1:22168/149‑1 (MQ=255)                                                                                                                    cgcACTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTgcgc                      >  1:764249/1‑151 (MQ=255)                                                                                                                       aCTGGTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaa                   <  2:782037/151‑1 (MQ=255)                                                                                                                          ggTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCgaagaa                <  2:2100/151‑1 (MQ=255)                                                                                                                           gTAGAAATCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGaaa               <  1:1009330/151‑1 (MQ=255)                                                                                                                               aaaTCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGCAGAAATc             >  2:877493/1‑149 (MQ=255)                                                                                                                                aaTCTACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGa           >  1:78570/1‑150 (MQ=255)                                                                                                                                    tACCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGtt      >  1:977715/1‑151 (MQ=255)                                                                                                                                     aCCGCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGtt      <  1:347565/150‑1 (MQ=255)                                                                                                                                        gCATGATGCGCTCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCgg  >  2:763552/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                            CTAAGCTGAGCCAGTCTGGTCACAAGCGTATCGAAACGCTGTTCACCAACGATCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCTGAAAGCCTGTTCGAGAACCTGTTCTTCTCCGAAGACCGTTATGACTTGTCTGCGGTTGGTCGTATGAAGTTCAACCGTTCTCTGCTGCGCGAAGAAATCGAAGGTTCCGG  >  NC_000913/4182212‑4182497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑