Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,325	C→A	A284A (GCG→GCT)	emrY ←	putative multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,325	0	C	A	100.0%	178.6 / NA	47	A284A (GCG→GCT)	emrY	putative multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/17);  total (30/17)

GTTGCCATAACGTCCTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTA  >  NC_000913/2481178‑2481469                                                                                                                                                    |                                                                                                                                                 gttgCCATAACGTCCTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAagcg                                                                                                                                               <  1:134332/151‑1 (MQ=255)        tAACGTCCTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCAtgtg                                                                                                                                         >  2:460082/1‑150 (MQ=255)        tAACGTCCTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCAtgtg                                                                                                                                         >  1:902273/1‑150 (MQ=255)         aaCGTCCTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGAt                                                                                                                                       <  2:253278/151‑1 (MQ=255)               cTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTcac                                                                                                                                 >  2:417232/1‑151 (MQ=255)                tATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTcac                                                                                                                                 >  2:459052/1‑150 (MQ=255)                tATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTcac                                                                                                                                 >  1:332595/1‑150 (MQ=255)                    aaaGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATAc                                                                                                                            >  1:636482/1‑151 (MQ=255)                     aaGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATAcc                                                                                                                           >  2:389567/1‑151 (MQ=255)                      aGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCa                                                                                                                          <  1:411925/151‑1 (MQ=255)                       ggTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCa                                                                                                                          >  1:143756/1‑150 (MQ=255)                             aTTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAATGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTTATACTCACAATACCAATGGTGa                                                                                                                   <  2:855259/151‑1 (MQ=255)                                aaTAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAg                                                                                                                 <  2:563430/150‑1 (MQ=255)                                    gTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACg                                                                                                            >  1:17729/1‑151 (MQ=255)                                           gatgCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACtt                                                                                                      >  1:501612/1‑150 (MQ=255)                                             tgCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTaa                                                                                                    >  1:1004370/1‑150 (MQ=255)                                              gCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTaaac                                                                                                  <  2:819153/151‑1 (MQ=255)                                                  aTGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTaaacaaac                                                                                              <  2:724259/151‑1 (MQ=255)                                                   tGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACt                                                                                             >  1:469653/1‑151 (MQ=255)                                                      gcgcATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTg                                                                                            >  1:884387/1‑149 (MQ=255)                                                       cgcATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTg                                                                                            >  2:67844/1‑148 (MQ=255)                                                        gcACAAGCAAGACCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTg                                                                                            >  2:487099/1‑147 (MQ=255)                                                             aGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGa                                                                                   <  1:169153/151‑1 (MQ=255)                                                               cAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCTCAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAAt                                                                                 <  2:198182/151‑1 (MQ=255)                                                                      ggCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTtc                                                                          >  2:567987/1‑151 (MQ=255)                                                                      ggCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTtc                                                                          >  2:312205/1‑151 (MQ=255)                                                                           atatCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGa                                                                     <  2:633500/151‑1 (MQ=255)                                                                                    tataCCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTc                                                            >  2:825086/1‑151 (MQ=255)                                                                                     ataCCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTcc                                                           <  1:915109/151‑1 (MQ=255)                                                                                          ccATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAAt                                                      <  2:982569/151‑1 (MQ=255)                                                                                                tttCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTa                                                 >  2:868631/1‑150 (MQ=255)                                                                                                  tCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTaa                                                >  2:134486/1‑149 (MQ=255)                                                                                                   ccTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTaa                                                >  1:258311/1‑148 (MQ=255)                                                                                                    cTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAag                                              >  1:9342/1‑149 (MQ=255)                                                                                                    cTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAag                                              >  2:54291/1‑149 (MQ=255)                                                                                                     tGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAag                                              >  1:926124/1‑148 (MQ=255)                                                                                                                cGGCATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAAt                                 >  1:1014009/1‑150 (MQ=255)                                                                                                                   cATAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACt                             <  2:452788/151‑1 (MQ=255)                                                                                                                     tAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTg                            >  2:176856/1‑150 (MQ=255)                                                                                                                     tAAGGACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTg                            >  2:129427/1‑150 (MQ=255)                                                                                                                         gACGATCGCTCCAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGAtact                       >  1:234366/1‑151 (MQ=255)                                                                                                                                   ccAGAGTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAata             <  1:946013/151‑1 (MQ=255)                                                                                                                                      gagTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAAtatta          <  1:954542/151‑1 (MQ=255)                                                                                                                                       agTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAAtattat         <  2:488008/151‑1 (MQ=255)                                                                                                                                        gTAAAATAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAAtattatt        <  1:825098/151‑1 (MQ=255)                                                                                                                                          aaagTAAATAAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAAtattattat      >  2:115678/1‑151 (MQ=255)                                                                                                                                             ataaataaGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGt   >  1:682885/1‑151 (MQ=255)                                                                                                                                              taaataAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGt   >  1:84661/1‑150 (MQ=255)                                                                                                                                               aaataAGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTa  <  1:975123/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GTTGCCATAACGTCCTATCAAAGGTGAAATTAATAGTGGCATGATGCCGATGGGCGCATAAGCAAGTCCGGCCCATATCGCATTATACCCCATCGTTTCCTGGAGTAACTGCGGCATAAGGACGATCGCTCCAGAGTAAAATAAATACGCGCATGTGATACTCACAATACCAATGGTGAAGTTACGGGACTTAAACAAACTGAGATCAAGAATCGGGTTCTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTA  >  NC_000913/2481178‑2481469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑