Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,478,324 | 0 | T | G | 43.9% | 91.1 / 15.3 | 57 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
Reads supporting (aligned to +/- strand): ref base T (6/26); new base G (25/0); total (31/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.52e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTT > NC_000913/1478175‑1478473 | gACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTg < 1:347407/151‑1 (MQ=255) aCTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGa < 2:639051/151‑1 (MQ=255) cGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGtt < 1:361288/151‑1 (MQ=255) gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGt < 1:785255/151‑1 (MQ=255) tGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAAAGGGGGGGAAA‑AGGGGGGAGGGTTCACTACGtct > 1:139054/1‑149 (MQ=255) gTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTAtt < 1:872428/151‑1 (MQ=255) tCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCCGGGAGAAAA‑AGGGGGGGGGGTACATTTCGTAtt > 2:932279/1‑150 (MQ=255) gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTATCGGGGAGAAAA‑AGGGGGGAGGGTACATTTCGTATTCg > 1:330438/1‑149 (MQ=255) gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTa < 1:705519/151‑1 (MQ=255) gtgtGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTAcc < 2:915179/151‑1 (MQ=255) gtgtGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTAcc < 2:201290/151‑1 (MQ=255) gCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCTGGGGGAAAAGGGGGGGGGGGTTTCTTTA‑GTATTCGTACCt > 1:185467/1‑148 (MQ=255) tcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCtggtgg < 2:887477/150‑1 (MQ=255) tcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCtggtg < 1:408309/149‑1 (MQ=255) tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACCGG‑GAGAAAA‑GGGGGGGGGGGTTCATTTCGTATTTTTACCTTGTggggg > 1:818585/1‑149 (MQ=255) aTGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCAGCGGGGAAA‑AGGGGTGGGGTTTTAATATCTATTTGTACCtgggtggg > 1:116348/1‑143 (MQ=255) aGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGt < 2:677257/151‑1 (MQ=255) aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAGAAAA‑AGGGGGGGGGGTATATTTCGTATTCGTACCTGGTGGGGCGTCGTCATTTGtt > 1:494707/1‑151 (MQ=255) aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGtt < 2:835393/151‑1 (MQ=255) aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCCGAGAGGAAA‑AGGGGGGGGGGTACATTTCGTATTTGTACCTTGGGGGGGGTCCTCAtttttt > 1:658040/1‑151 (MQ=255) tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGAGAGAAAA‑GGGGGGGGGGGTTAATTTAGTTTTTGTAACTTGGGGGGGATCGGCATTTGtttt > 1:109592/1‑151 (MQ=255) ataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACCGG‑GAGAAAA‑GGGGGGGGGGGTAAACTTAACATTTGTATCTTGTGGGGGATTGTCCTTTGttttt > 2:768944/1‑151 (MQ=255) aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑AGGGGGGGGGGTACATTACGTATTCGTACCTTGGGGGGGATCGTCATtttttttt > 2:160607/1‑149 (MQ=255) tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGGGAGAAAA‑AGGGGGGGGGGTTCATTTAGTATTTGTACCTGGTGGGGGATCGTCCTttttttttt > 2:395772/1‑147 (MQ=255) tGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAGAAAA‑GGGGGGGAGGGTACATTTCGTATTTGTACCTGGGGGGGGATCGTCCTTTTTTTTTTaa > 2:409832/1‑150 (MQ=255) cTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGGGGGAAAA‑AGGGGGGAGGGTTAATTACGTTTTCGTACCTTGTGGGGGGTTGTCATttttttttt > 1:380636/1‑145 (MQ=255) aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑ATTACGGGGGGAAAA‑AAGGGGGAGGATAACATAAATAATTGTTCCTGGGGGGGGATCGGTATTTGttctttcttt > 1:1613/1‑141 (MQ=255) ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGGGAAAA‑AGGGGGGGGGGTACATTACGTATTCGTACCCGGTGGGGGATCGTCATTTTTTTTTTTATTGGGAtttttgg > 2:6603/1‑147 (MQ=255) ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAgg < 1:641299/149‑1 (MQ=255) aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑AGGGGGGGGGGTACATTACGTATTTGTACCTGGGGGGGCATCGTCATTTGTTTTTTAATGGTGGTTTTAGGTCCACGc > 2:653170/1‑151 (MQ=255) tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGa < 2:250103/149‑1 (MQ=255) tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGa < 1:545545/149‑1 (MQ=255) ttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTTCTGTGAGAAAA‑GGGGGGGGGGGTACATTACGTATTCGTAACCGGGGGGGCGTCGGCATTTGTTTTTTAATGGGGGTTTTTGGTCCACGCTgggt > 2:527426/1‑147 (MQ=255) cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgac < 1:953665/150‑1 (MQ=255) gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacgt < 2:669211/151‑1 (MQ=255) gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGGGAGGGTACATTACGTATTCGTACCTGGGGGGGGATCGTCCTTTTTTTTTTAATGGTGATTTTAGGTCCACGCTGGGtgacgt > 2:623281/1‑151 (MQ=255) cgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacgtt < 2:330442/151‑1 (MQ=255) gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAGAAAA‑AGGGGGGAGGGTAAATTTCCTATTCGGTCCTGGGGGGGCGTCGTCATTTGTTTTTTTATGGTGGTTTTTGGTCCACGCTGGAtgacgtt > 1:319861/1‑150 (MQ=255) gttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacgttgacg < 1:34999/151‑1 (MQ=255) ttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGGGAAAA‑GGGGGGGAGGGTAAATTACGGATTTGGACCTGGTGGGGGCTCGGCATTTTTTTTTCAATGGGGATTTTAGGGCCACCCTGGGtgacgttttcg > 2:796031/1‑146 (MQ=255) aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATgacgttgacgtt < 1:108963/150‑1 (MQ=255) aTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGTGAAAA‑AGGGGGGGGGTTACAATACGTATTTGTACCTGGTGGGGGCTCCGCATTTTTTTTTTTATGGTGGTTTTAGGGCCCCGCTTGATTACGTTGGCGgttt > 2:776242/1‑151 (MQ=255) ttttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGGGAGAAAA‑GGGGGGGAGGGTACATTTTGTATTTGTAACCGGTGGGGGATTGTCCTTTGTTTTTTAATGGTGGTTTTTGGTCCCCGCTGGATGACGTTGAAGttttt > 1:322811/1‑151 (MQ=255) aCGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAGGAAA‑GGGGGGGGGGGTATATTACGTATTCGTACCTGGGGGGGGATCGGCATTTTTTTTTTAATGGGGGTTTTTGGGCCACGCTGGGTGACGTTGACGtttttt > 1:175836/1‑144 (MQ=255) aTAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGGGAGGGTACAATACTTTTTTGTTCCTTGGGGGGGATCGTCCGTTTTTTTTTTATTGGGGTTTTAGGGCCACGCTGGGTGAAGTTTACGTTTTTTTTGCTggt > 1:411672/1‑144 (MQ=255) ccGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTg < 1:726512/151‑1 (MQ=255) cGTTTTCTGCTATGGCGG‑TTTACCGAGGGAAAA‑GGGGGGGAGGGTAAATTACGTATTCGTACCCGGTGGGGGCTCGTCATTTTTTTTTTTATGGGGGTTTTAGGGCCCCGCTGGATGACGTTGTCGTTTTTTTCGCTGATAAGtttt > 1:830387/1‑147 (MQ=255) ggCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat < 2:139054/150‑1 (MQ=255) aCCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg < 2:844748/151‑1 (MQ=255) agagaAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat < 2:185468/151‑1 (MQ=255) gagaAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata < 2:700970/151‑1 (MQ=255) gagaAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat > 1:224484/1‑150 (MQ=255) aaaa‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt > 1:218114/1‑151 (MQ=255) aaa‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg > 2:512666/1‑151 (MQ=255) a‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt > 2:736523/1‑150 (MQ=255) gggTGAGGGTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAt > 1:903070/1‑151 (MQ=255) gTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAttt < 1:532185/151‑1 (MQ=255) | GACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAGAAAA‑GGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTT > NC_000913/1478175‑1478473 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |