Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,578,143 | 0 | A | C | 42.6% | 91.4 / 13.8 | 55 | L79V (TTA→GTA) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
Reads supporting (aligned to +/- strand): ref base A (23/8); new base C (0/23); total (24/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.37e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTG > NC_000913/2577997‑2578289 | ttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑cccc > 2:378765/1‑151 (MQ=255) cGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCa < 1:691542/150‑1 (MQ=255) gAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGc < 2:721852/151‑1 (MQ=255) gAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGc < 2:297466/151‑1 (MQ=255) cggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCAccgc > 1:903849/1‑151 (MQ=255) cggcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCAccgc < 2:859124/151‑1 (MQ=255) gcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCATGTTCAAATACATCAAGCCCGGCGAATTTCTT‑AAAC‑AGAC‑CGCCCTTCCCTTCCATCACGGC‑TTGG‑CCTG‑CCAGGGCGCCAATGTAGCCTA‑A‑CCCCTGCCccgccg < 1:268987/151‑1 (MQ=255) cgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGt > 2:58620/1‑151 (MQ=255) gACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGt > 2:709781/1‑150 (MQ=255) aaTAAATTTGCCGGGGTCGAGTCCGTCAACTTAAATGTAAAATACACCAACCCCGGCAATTTTCTA‑AAAC‑AGAC‑CCCCCTTCCCTCCCACCCCCGT‑TTTG‑CCTC‑CCAGCGCGCAAATGTGGCCTA‑C‑CCCCAGCACCGCCGTACCGTTa < 2:510795/148‑1 (MQ=255) aTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTag > 1:878895/1‑148 (MQ=255) aaaTTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTag > 2:21830/1‑146 (MQ=255) gTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGcc > 1:92609/1‑151 (MQ=255) gggTGGTGTTCTCCAACTTGAATGCAAAATACGCAAATCCGGGAGAATTTTTA‑AAAC‑AAAA‑CCCCTTTGCTTCCCACCCCTGG‑TTTG‑CCTC‑CCGCCGC‑CCAACGTTGCCCA‑ACCCCCGGCTCCGCCGTACCGTTAGAGATCACCGccacc < 1:468924/150‑1 (MQ=255) tCGAGTTCGTCACCTTAAATTTAAAATCCAACACCCCGGGCGAATTTCTA‑AAAC‑AGAC‑CGCCCTTCCCTCCCACCACGGT‑TTTG‑CCTG‑CCAGCGCCCAAATTTTCCCTA‑C‑CCCAAGCACCGCCGTACCGTTAGAGATCACCGccacca < 1:1000105/148‑1 (MQ=255) gAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt > 1:206691/1‑150 (MQ=255) tttCGTCAATTTCAATGTAAAATACACCCCTCCCGGCGAATTTCTA‑AAAC‑AACC‑CCCCCTTCCTTTCCACCCCCGT‑TTTG‑CCGG‑CCCGCGCGCATATGTTGCCTA‑C‑CCCCGGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTa < 1:134065/148‑1 (MQ=255) accTTCAATGCAAAAAACATCAACCCCGGCGAATTTCTA‑AAAC‑AGAC‑CGCCCTTCCCTTCCACCCCCGT‑TTTG‑CCTG‑CCGCCGCGCCAATTTTGCCAA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCggg < 2:196247/147‑1 (MQ=255) cTTAAATGTCAAATACATCACCCCGGGCAAATTT‑TT‑AAACAAAAA‑CCCCCTTCCCTCCCA‑CCCCGG‑TTTG‑GCCG‑CCCGGCCCCCCATTTTTCCAA‑ACCCCCGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCg < 2:938859/149‑1 (MQ=255) ttCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGt > 2:512162/1‑150 (MQ=255) aaTGTCAAATACACCAACCCCGGCGAATTTCTA‑AAAC‑AGAC‑CGCCCTTCCCTTCCACCCCCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑C‑CCCAAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat < 2:973122/149‑1 (MQ=255) tGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAttt > 2:917692/1‑151 (MQ=255) tCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt > 2:555838/1‑151 (MQ=255) aaaTACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt > 2:149942/1‑149 (MQ=255) aaatacaccaccccGGGCAATTTCCT‑AAAA‑AAAACCCCCCTTCCCTCCCACCCCCGT‑TTTG‑CCTC‑CCGGCGCCCATATTTTCCCTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAttt < 1:530265/136‑1 (MQ=255) ccccGGCAATTTTCTA‑AAAC‑AG‑A‑CCCCCTTCCCCTCCCACCCCCG‑TTTGCCCCG‑CCCGCGC‑CCCATGTTCCCTACC‑CCCCCGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACg < 1:605280/149‑1 (MQ=255) cccGGAGATTTTTTA‑AAAC‑AAAC‑CCCCCTTCCCTCCCACCCCGGG‑TTGG‑CCGC‑CCAGACCCACAATGTTGCATA‑C‑CCCAGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc < 1:379411/148‑1 (MQ=255) ccGGGGAATTTCTTAAAAA‑AGAC‑CCCCTTTCCCCCCCATCCCCGT‑TTTC‑CCGC‑CCGCCGCCCAAATTTGCCCTC‑C‑CCCCGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCttt < 1:645972/144‑1 (MQ=255) ccGGCTAATTTATA‑AAAC‑AAAA‑CG‑CCTTTCCCTCCATCCCCCG‑TTTGCCCTC‑CCAGCGCGCAAATTTTGCCTC‑C‑CCCAAGACCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCtttt < 2:630061/144‑1 (MQ=255) gAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtttt > 2:1012915/1‑151 (MQ=255) tttCTA‑AAAC‑AGAC‑CCCCTTTCCCTCCCCTCCCCGG‑TTTC‑CCTC‑CCAGCGCCCAAATTTGCCCTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTc < 1:123899/150‑1 (MQ=255) tttCTA‑AAAA‑AGAC‑CCCCTTTCCCTTCC‑TCCCCGGTTTTC‑CCCC‑CCAGCCCCCCAATGTTGCCTA‑C‑CCCCGCCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTc < 1:108728/150‑1 (MQ=255) ttCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCg > 1:541440/1‑150 (MQ=255) ttCTA‑AAAC‑AGAC‑CCCCCTTGCCTTCAACCCCGGT‑TTTG‑CCTG‑CAAGCGCGCCATTGTTCAAAA‑C‑CCCCAGCTCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCg < 2:21537/150‑1 (MQ=255) tCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAt > 1:435385/1‑151 (MQ=255) aaaaC‑AGAA‑CCCCCTTGCTTCCCACCCCGGG‑TTTG‑CCTG‑CCGCCCCCCTATTGTGGCCTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTc < 1:784297/150‑1 (MQ=255) aC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa > 2:480820/1‑149 (MQ=255) aC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCa > 1:25728/1‑149 (MQ=255) aGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGAc > 2:142614/1‑151 (MQ=255) cGCCCTTCCCTTCCACCCCGGG‑TTGG‑CCCG‑CAAGCCCGCAAATTTTCCCAA‑A‑CCCAAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAgg < 2:891095/151‑1 (MQ=255) gCCCTTGCCTTCCGCAACAGC‑ATTA‑TCTG‑CTATCACGCCAATCTGGCGGA‑T‑CCCCAGCACCGCCGTACCGTGAGAGACCTCCGCCACCAGGTTACCTCGGGCCGTATATCTGAAGGCTTTTAACGGGTCTTTTTCGACTTCAAGACAAgg > 1:492966/1‑150 (MQ=255) gCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGt > 2:144350/1‑151 (MQ=255) cctttcccTCCCACCCCCGT‑TTTG‑CCTG‑CCGGCGCGCAATTTTTCCCTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGa < 1:336034/138‑1 (MQ=255) ttcccTCCCACCCCCGT‑TTTG‑CCTG‑CCGGCGCCCCATTGTTCCCTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc < 1:225129/146‑1 (MQ=255) tccTTCCCACCCCGG‑TTTT‑CCCGCCCGGCGCCCAAATGTCCCCAA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCgg < 2:981811/149‑1 (MQ=255) ccccccccGG‑TTGC‑CCTC‑CCGCCGCGCAATTTTTGCCAA‑C‑CCCCCGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAAc < 1:822777/142‑1 (MQ=255) ctccGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACACCTgg > 1:164755/3‑150 (MQ=255) ccccg‑TTTGCCCTC‑CCGGCCCGCCAATGTCGCATA‑C‑CCCCATCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACg < 1:189997/141‑1 (MQ=255) gg‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAg > 1:753034/1‑150 (MQ=255) gg‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGt > 2:541738/1‑151 (MQ=255) cAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg > 2:992638/1‑151 (MQ=255) attGTTGCCAA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAg < 2:564744/142‑1 (MQ=255) attGTTCCCAA‑C‑CCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcg < 2:656555/146‑1 (MQ=255) ttGCCTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCt < 2:773341/148‑1 (MQ=255) ccTA‑C‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTg < 1:199827/151‑1 (MQ=255) | TTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAAC‑AGAA‑CGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTG‑CCAGCGCGCCAATGTTGCCTA‑A‑CCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTG > NC_000913/2577997‑2578289 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |