Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,332,791 | 0 | A | C | 44.6% | 88.1 / 20.3 | 56 | V161G (GTG→GGG) | basS | sensory histidine kinase in two‑component regulatory system with BasR |
Reads supporting (aligned to +/- strand): ref base A (29/2); new base C (0/25); total (29/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.30e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGG > NC_000913/4332642‑4332939 | cATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCAc > 2:702042/1‑151 (MQ=255) tGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCAccc > 1:567840/1‑151 (MQ=255) tGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCAccccc < 1:280281/150‑1 (MQ=255) gATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGc > 1:2552/1‑151 (MQ=255) tACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTgg > 2:929753/1‑151 (MQ=255) ccGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCg > 2:954288/1‑151 (MQ=255) aaaaaaaTGCCTGCCGGCCCCCCGCCTGTTGCGGCGGTGGGGAGACGCTCCCCATCATCTAATCAGCCCGTGCCACTAACGGTGCTACATAAAT‑GTGAGGCGTTTTCGCC‑AGCGGTTCCAGATGAAAA‑CCCCCCCCCGCCAGTGGCGTTCg < 1:370562/148‑1 (MQ=255) aaaTGACTGTCCGCCCCGCCCCAGTTGCAGCAGCGGGGAGCCCCTCCCCATCTTCTGATCAGCCCGTGCCCCTAACGGTGCTACATAATT‑TTGATGTTTTTCCGCA‑AGCGGTCCCAAATGCAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGt < 2:115243/151‑1 (MQ=255) aatcccgccccGGAACGCCCCAGTTGAAGCCGTTGGGACACCCTCTCCATCCCCTGATCAATCCGTGCCAATATCGGTTCTAAATAAAT‑TTGATGCGTTTTCGCC‑CGCAGTACAACACAAAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGtt < 2:661525/142‑1 (MQ=255) tGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCg > 1:877211/1‑151 (MQ=255) tCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGcgcg > 2:214080/1‑151 (MQ=255) ccGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGcgcg > 2:506874/1‑150 (MQ=255) ccggTTGCAGCAGCGGGGAGCCCCTCCCCATCATCTGACCAGGCCGTCCCACCAACGGTGCTACATCAAT‑GTGATGCTTTTCCGCC‑AGCAGTCCCAGATCCACA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTc < 1:732164/142‑1 (MQ=255) ccATTTGCAGCGGGTGGGAGCCTCTCCCCAACATCGAATCAAGCGGTCCCATTAACGGTGTCAAATAAGT‑GTGATGGGTTTTCGCC‑AGCGGTCCAAGATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCgg < 2:615746/150‑1 (MQ=255) aGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTa > 1:996357/1‑150 (MQ=255) cagcagCTGGGAGACGCTCTCCATCATCTGATCTAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaac > 2:399918/1‑151 (MQ=255) gcggggAGCCCCTCTCCATCATCTGACCAAGCGGTCCCACAAACGGTGCTACATCAAT‑GTGATGCTTTTTCGCC‑AGCATTCCCAGAGGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCt < 1:960526/145‑1 (MQ=255) gcTGGGGAACGCTCTCCATCATTTGATCAAGCCGTCCCACTAAGGGTGCTAAATCAAT‑GTGTTGCGTTTTCGCC‑AGCGGTCCCAGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTc < 2:418070/151‑1 (MQ=255) tggggcccGCTCTCCAACTTCTGATCAACCGGTCCCACTAACGGTCCTACATCAAG‑GGGATG‑GTTTCCCCCAAGCTGTCCCAGATCAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTc < 1:187452/142‑1 (MQ=255) gggAGACCCTCCCCATCATCTGATCAAGCCGTGCCACTAAGGGTGCTACATCAAT‑GTGATGCTTTTTCGCC‑AGCATTCCAAGATGCAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAaacaac < 2:328784/143‑1 (MQ=255) gaCGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGCTGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGCGGCGTTCGCAGTTCGTGCGCGACGTCACCGCTAAACAACCTTTCGTGATcc > 1:757536/1‑151 (MQ=255) catcctCTAAGCAAGCCGCCCCACTAGGGCAGCTACATCAGT‑GTGCTGCTTTTCCCCC‑AGGATTTCCAAATCCACA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGt < 1:48099/147‑1 (MQ=255) atcatcTGATCAAGCCGTGCCACTAACGGTGTTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTgg > 2:749465/1‑151 (MQ=255) cTGATCAAGCCGGGCCTCCAACGGTGCTACAGAAAT‑GTGATGCTTTTTCCCC‑AGCTGTTCCAGATACACA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTc < 1:710775/148‑1 (MQ=255) aTCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCg > 2:700508/1‑150 (MQ=255) aGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg > 2:986988/1‑150 (MQ=255) aGCCGTGCCACTAACGGAGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCTTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg > 1:191726/1‑150 (MQ=255) ccggcccACTAACGGTGCTCCATCAAT‑GTGATCTTTTTGCCCC‑AGCAGTTCCAGACGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGa < 1:221665/144‑1 (MQ=255) ccGTCCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACATCCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTg < 2:537201/148‑1 (MQ=255) cGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGAcc > 1:540856/1‑150 (MQ=255) gccccACTAGCGGTCCAACA‑CAATGGTGATGGTTTTCCCCC‑AGCATTCCCAGATGAAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCa < 1:728765/147‑1 (MQ=255) accGGTGCTCCACCAAT‑GTGCTGTGTTTCCGCC‑AGCTGTTCCAGACGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCg < 1:96979/145‑1 (MQ=255) ggggccacttCAAT‑GTGATGCTTTTCCCCC‑AGCATTGCTAGATGAAAA‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACg < 2:684391/142‑1 (MQ=255) ggTGCCACACCATT‑GTATTGCTTTTTCGCC‑AGCAGTTCCAGAAGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATc < 2:373436/143‑1 (MQ=255) tGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGcc > 1:39710/1‑151 (MQ=255) gCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCg > 2:803267/1‑151 (MQ=255) aCATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGaa > 2:446065/1‑150 (MQ=255) aattggaggCGTTTCCCCC‑AGCAGTTCAAGA‑GCAAACCCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAacca < 2:498488/141‑1 (MQ=255) t‑gtgATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGc > 1:23743/1‑151 (MQ=255) gATGCTTTTCCCCA‑AGAAGTTCCAGAAACAAG‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCt < 2:317308/149‑1 (MQ=255) aTGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCg > 1:300935/1‑151 (MQ=255) cGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAtt > 2:9098/1‑151 (MQ=255) gTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGAttt > 2:637212/1‑151 (MQ=255) ttttCCCC‑AGCGTTCCCAGATCAAAA‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTc < 1:599828/145‑1 (MQ=255) tttcCGCC‑AGCTTTTCCAGAGGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGa < 2:138608/147‑1 (MQ=255) ttttctcgggcttctCCAGACGAAAG‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGa < 1:392128/133‑1 (MQ=255) tttCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCg > 2:1001738/1‑151 (MQ=255) cGCC‑AGCGGTCCCAGATGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCg < 2:199760/148‑1 (MQ=255) c‑agcagTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTg > 2:476115/1‑151 (MQ=255) cagTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgcg > 2:10007/1‑151 (MQ=255) caatcccAGATGCAAC‑CCCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGgc < 1:702031/145‑1 (MQ=255) cAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCtgtg > 1:112682/1‑150 (MQ=255) aTGCAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATg < 2:864774/151‑1 (MQ=255) aTGAAAC‑CGCCCCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAAt < 2:911681/150‑1 (MQ=255) cAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGc > 1:912966/1‑150 (MQ=255) gCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATggg > 2:873126/1‑151 (MQ=255) | CATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCACGCGCCAGTTGCAGCAGCTGGGAGACGCTCTCCATCATCTGATCAAGCCGTGCCACTAACGGTGCTACATCAAT‑GTGATGCGTTTTCGCC‑AGCAGTTCCAGATGCAAA‑CGCACCCCCGCCAGTGGCGTTCGCAGTTCGTGCGCGACGTCAGCGGTAAACAACCTTTCGTTATCCAGCGTGCTGGTCAGGCGACTGACCAGATCGTTTAACGCCGAAACCACCGCTTCGATTTCGAGGGTGGCGCTGTGAATGGCAATGGG > NC_000913/4332642‑4332939 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |