Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 99,891 C→T A83V (GCC→GTC)  murG → N‑acetylglucosaminyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091399,8910CT98.3% 212.7 / NA 59A83V (GCC→GTC) murGN‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (31/27);  minor base A (1/0);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCTT  >  NC_000913/99744‑100038
                                                                                                                                                   |                                                                                                                                                   
ggCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTg                                                                                                                                                  <  2:60467/151‑1 (MQ=255)
 gCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTgg                                                                                                                                                 >  1:988828/1‑151 (MQ=255)
        ccctggctggGGACTGCCGACCGTATGGAAGCGGGCTTAGTGACAAAACATGGCATCGAAATTGGTTTTATTCGTATCTCTGCTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGACCCGCTGCGTATCTTCAACGACAGGCGTCAg                                                                                                                                           >  1:423506/3‑150 (MQ=255)
        cGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAgg                                                                                                                                          >  1:542953/1‑151 (MQ=255)
        cGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAg                                                                                                                                           <  2:698319/150‑1 (MQ=255)
             gctggGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCgg                                                                                                                                     >  1:12163/1‑151 (MQ=255)
               tggGGACTGCCGCCCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGAGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGc                                                                                                                                   >  1:107538/1‑151 (MQ=255)
                ggggACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGc                                                                                                                                   <  2:174202/150‑1 (MQ=255)
                         cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaaa                                                                                                                         <  1:935134/151‑1 (MQ=255)
                         cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                          >  1:1002016/1‑150 (MQ=255)
                          gACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                          >  2:72039/1‑149 (MQ=255)
                          gACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAg                                                                                                                        <  2:687240/151‑1 (MQ=255)
                           aCCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGc                                                                                                                       <  1:997642/151‑1 (MQ=255)
                              gTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTa                                                                                                                    <  1:156870/151‑1 (MQ=255)
                                  ggAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACaa                                                                                                                 <  2:970615/150‑1 (MQ=255)
                                           cTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACgtg                                                                                                       >  1:201349/1‑151 (MQ=255)
                                             tAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACgtgg                                                                                                      >  2:211334/1‑150 (MQ=255)
                                                 gCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTc                                                                                                 <  2:701458/151‑1 (MQ=255)
                                                   cAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCgg                                                                                               <  2:630588/151‑1 (MQ=255)
                                                   cAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGAGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCgg                                                                                               >  1:118999/1‑151 (MQ=255)
                                                      aaCATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTAt                                                                                            >  1:285474/1‑151 (MQ=255)
                                                          tGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATggg                                                                                         >  2:451804/1‑150 (MQ=255)
                                                              aTCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCt                                                                                    <  1:748252/151‑1 (MQ=255)
                                                              aTCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCt                                                                                    <  1:981124/151‑1 (MQ=255)
                                                                cGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTAc                                                                                  >  2:744313/1‑151 (MQ=255)
                                                                 gAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACg                                                                                 >  2:71739/1‑151 (MQ=255)
                                                                 gAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACg                                                                                 <  2:424479/151‑1 (MQ=255)
                                                                    cttgatttgatTCGTATCTCTGGACTGAGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAAAGTCTGGCGTCAGTCGCGGGCGATTATGAAAGCGTACAAACCTGAAGTGGTGCTCGGCATGGGAGGCTCCgtgt                                                                              >  1:993597/2‑151 (MQ=255)
                                                                       gATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTc                                                                             >  1:2340/1‑149 (MQ=255)
                                                                            cATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTcc                                                                       <  1:374499/150‑1 (MQ=255)
                                                                            cATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTcc                                                                       >  2:584574/1‑150 (MQ=255)
                                                                                   aTCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTc                                                               >  1:602867/1‑151 (MQ=255)
                                                                                       ctGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGc                                                           >  2:657332/1‑151 (MQ=255)
                                                                                              gCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTg                                                     <  2:607908/150‑1 (MQ=255)
                                                                                                gTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTc                                                  <  2:13378/151‑1 (MQ=255)
                                                                                                 tGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCg                                                 >  2:241079/1‑151 (MQ=255)
                                                                                                     aaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGtt                                               >  1:994745/1‑149 (MQ=255)
                                                                                                     aaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAg                                             <  2:801203/151‑1 (MQ=255)
                                                                                                     aaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAg                                             <  2:12163/151‑1 (MQ=255)
                                                                                                      aaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAgg                                            >  1:900359/1‑151 (MQ=255)
                                                                                                       aGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGc                                           <  2:80831/151‑1 (MQ=255)
                                                                                                        ggTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCa                                          <  1:443117/151‑1 (MQ=255)
                                                                                                            taAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTcc                                      >  2:291603/1‑151 (MQ=255)
                                                                                                             aaaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCg                                     >  1:247927/1‑151 (MQ=255)
                                                                                                             aaaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCg                                     <  1:996013/151‑1 (MQ=255)
                                                                                                              aaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCgg                                    >  1:1004724/1‑151 (MQ=255)
                                                                                                              aaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCgg                                    >  2:436235/1‑151 (MQ=255)
                                                                                                                 gCAGTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGACGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGtt                                  <  1:973047/150‑1 (MQ=255)
                                                                                                                     tGATAACTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACt                             >  2:399180/1‑151 (MQ=255)
                                                                                                                            tGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGa                       >  2:697343/1‑150 (MQ=255)
                                                                                                                             gCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGa                       <  2:848623/149‑1 (MQ=255)
                                                                                                                              ccccGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAAc                     >  2:445214/1‑150 (MQ=255)
                                                                                                                                  gCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAAc                <  1:557367/151‑1 (MQ=255)
                                                                                                                                              cAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCggg     <  2:99374/150‑1 (MQ=255)
                                                                                                                                               aaCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCt   >  2:463462/1‑151 (MQ=255)
                                                                                                                                               aaCGTCTGGCGGCAGGTGCGGGCGATTATGAAAGCGTACAAGCCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTCGGCATTCCGGTTGTACTTCATGACCAAAACGGTATTGCGGGc    <  1:379116/150‑1 (MQ=255)
                                                                                                                                                aCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCtt  <  1:72039/151‑1 (MQ=255)
                                                                                                                                                aCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCtt  >  1:692625/1‑151 (MQ=255)
                                                                                                                                                aCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCtt  >  1:113246/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCTT  >  NC_000913/99744‑100038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑