Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,025,248 +TC intergenic (+35/‑263) yodD → / → yedP uncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,2461.T89.9% 251.2 / 19.0 69intergenic (+33/‑265)yodD/yedPuncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base T (23/39);  total (26/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
*NC_0009132,025,2462.C89.7% 240.8 / 20.0 69intergenic (+33/‑265)yodD/yedPuncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base C (22/39);  total (26/43)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑TCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGA  >  NC_000913/2025100‑2025385
                                                                                                                                                   ||                                                                                                                                           
aaCGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctc                                                                                                                                         >  2:129126/1‑149 (MQ=255)
 aCGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcg                                                                                                                                        <  2:306084/151‑4 (MQ=255)
      tCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGGACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATCAAAAAATCCCGACCCTGAGGGGG‑‑Tctagggatg                                                                                                                                   >  1:1165122/1‑143 (MQ=255)
      tCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcgggatg                                                                                                                                   >  1:489798/1‑143 (MQ=255)
      tCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcgggat                                                                                                                                    <  2:1021332/150‑8 (MQ=255)
         gAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcgggatga                                                                                                                                  <  1:1264474/149‑10 (MQ=255)
         gAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcgggatga                                                                                                                                  <  2:769545/149‑10 (MQ=255)
              cGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACtt                                                                                                                               <  2:452129/149‑1 (MQ=255)
               gTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCt                                                                                                                            <  1:989100/151‑1 (MQ=255)
                  aGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTaa                                                                                                                         <  2:756902/151‑1 (MQ=255)
                   gCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAg                                                                                                                        <  1:673832/151‑1 (MQ=255)
                     gTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCa                                                                                                                      <  2:946706/151‑1 (MQ=255)
                       cGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCa                                                                                                                      >  1:641772/1‑149 (MQ=255)
                        gATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCaaga                                                                                                                   <  1:1039227/151‑1 (MQ=255)
                         aTGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCaagaa                                                                                                                  <  1:649160/151‑1 (MQ=255)
                                tGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACtt                                                                                                            >  1:862763/1‑150 (MQ=255)
                                 gAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTa                                                                                                           >  1:601657/1‑150 (MQ=255)
                                  aaTATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTaaa                                                                                                         <  2:81544/151‑1 (MQ=255)
                                  aaTATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTaa                                                                                                          >  2:1104975/1‑150 (MQ=255)
                                     atCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTaaaaa                                                                                                       <  2:489806/150‑1 (MQ=255)
                                      tCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAAt                                                                                                     >  2:1037068/1‑151 (MQ=255)
                                      tCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAAATTGCTTAAGCAAGAAGCACTTaaaaaa                                                                                                      >  2:808539/1‑150 (MQ=255)
                                       cATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAAtt                                                                                                    <  1:562606/151‑1 (MQ=255)
                                       cATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAAtt                                                                                                    <  1:197968/151‑1 (MQ=255)
                                        aTACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTc                                                                                                   <  1:179776/151‑1 (MQ=255)
                                              ggCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTac                                                                                              >  2:155587/1‑150 (MQ=255)
                                                 gTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTATCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTacac                                                                                            >  1:961475/1‑149 (MQ=255)
                                                   gAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAg                                                                                         <  2:516245/150‑1 (MQ=255)
                                                     aTTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGaa                                                                                      <  2:1088707/151‑1 (MQ=255)
                                                               gCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAtgtg                                                                            <  1:703053/151‑1 (MQ=255)
                                                                    cGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTtcat                                                                       <  1:1087625/151‑1 (MQ=255)
                                                                    cGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTtcat                                                                       <  1:751104/151‑1 (MQ=255)
                                                                    cGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTtcat                                                                       <  1:388908/151‑1 (MQ=255)
                                                                     gAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTtcatc                                                                      <  2:857565/151‑1 (MQ=255)
                                                                       aCTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCAc                                                                    <  2:446020/151‑1 (MQ=255)
                                                                          ggATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCtt                                                                 <  1:1238978/151‑1 (MQ=255)
                                                                          ggATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCtt                                                                 <  1:389438/151‑1 (MQ=255)
                                                                              atTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATcc                                                             <  1:935854/151‑1 (MQ=255)
                                                                                tCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCg                                                            <  1:875130/150‑1 (MQ=255)
                                                                                tCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGc                                                           >  2:64070/1‑151 (MQ=255)
                                                                                 cATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCa                                                          <  1:514012/151‑1 (MQ=255)
                                                                                   tGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAt                                                        >  2:395372/1‑151 (MQ=255)
                                                                                    gACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAt                                                        <  2:1173048/150‑1 (MQ=255)
                                                                                     aCTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttt                                                      <  2:260821/151‑1 (MQ=255)
                                                                                       ttCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttt                                                    >  2:360853/1‑151 (MQ=255)
                                                                                         cAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttttt                                                  >  1:94131/1‑151 (MQ=255)
                                                                                         cAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttttt                                                  >  1:26776/1‑151 (MQ=255)
                                                                                             gTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCt                                              <  1:236046/151‑1 (MQ=255)
                                                                                                tctGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGAc                                           >  2:1245833/1‑151 (MQ=255)
                                                                                                 ctGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTg                                             <  2:529559/148‑1 (MQ=255)
                                                                                                    aaGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGa                                       <  2:444635/151‑1 (MQ=255)
                                                                                                            cGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTc                               <  2:1002912/151‑1 (MQ=255)
                                                                                                            cGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTc                               <  2:218034/151‑1 (MQ=255)
                                                                                                              ttGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGc                             >  2:361315/1‑151 (MQ=255)
                                                                                                                  aTGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCata                         <  1:294561/151‑1 (MQ=255)
                                                                                                                  aTGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCat                          >  1:1031840/1‑150 (MQ=255)
                                                                                                                    gCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCatat                        <  2:601665/150‑1 (MQ=255)
                                                                                                                     cacaATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatga                      >  1:414031/1‑150 (MQ=255)
                                                                                                                         aTAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatgaatg                   >  1:282119/1‑150 (MQ=255)
                                                                                                                           aaaaaaaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                >  1:834405/1‑151 (MQ=255)
                                                                                                                           aaaaaaaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                <  1:909253/151‑1 (MQ=255)
                                                                                                                             aaaaaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTaa              <  2:980107/151‑1 (MQ=255)
                                                                                                                               aaaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACg            >  2:80077/1‑151 (MQ=255)
                                                                                                                                aaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGc           >  2:59717/1‑151 (MQ=255)
                                                                                                                                 aTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCt          <  2:1176017/151‑1 (MQ=255)
                                                                                                                                  tCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCGCCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCt          >  2:131524/1‑150 (MQ=255)
                                                                                                                                    ccGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACt       <  1:554977/151‑1 (MQ=255)
                                                                                                                                       aCCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt    >  2:119998/1‑151 (MQ=255)
                                                                                                                                         ccTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGa  <  1:925939/151‑1 (MQ=255)
                                                                                                                                                   ||                                                                                                                                           
AACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑TCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGA  >  NC_000913/2025100‑2025385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: