Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	941,713	0	T	G	47.9%	55.3 / 17.3	48	V252G (GTG→GGG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (3/22);  new base G (23/0);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 8.48e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941570‑941862                                                                                                                                                |                                                                                                                                                      ctcctcCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                                  >  2:348533/1‑149 (MQ=255)       cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCtt                                                                                                                                          <  1:892035/151‑1 (MQ=255)       cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCtt                                                                                                                                          >  2:1096774/1‑151 (MQ=255)         cgcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTTTATCtt                                                                                                                                          >  2:1019587/1‑149 (MQ=255)                 gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTACTATCTTTAAAAgg                                                                                                                                   >  1:599151/1‑148 (MQ=255)                      aGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAg                                                                                                                           <  2:467368/151‑1 (MQ=255)                         tttGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAAGCACGCCAGaa                                                                                                                         >  2:147416/1‑150 (MQ=255)                           tGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCCTACTATCTTTAAAAAGCACGCCCGaaaa                                                                                                                       >  1:462143/1‑149 (MQ=255)                            gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCt                                                                                                                     <  2:546582/151‑1 (MQ=255)                              aCTATACCTACGGCGGCTTGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTAGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaaa                                                                                                                        <  1:463711/146‑1 (MQ=255)                                tataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTTATATCTTTAACAAGCACGcccacaaactaa                                                                                                                   >  1:1197165/1‑139 (MQ=255)                                  taCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACGCCCGAAAACTaaatcc                                                                                                               >  2:1188579/1‑147 (MQ=255)                                   aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAATCTAATGccc                                                                                                              >  2:912789/1‑151 (MQ=255)                                                gggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcat                                                                                                 <  1:970451/151‑1 (MQ=255)                                                gggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatc                                                                                                   <  1:119341/149‑1 (MQ=255)                                                  gCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAACAGGCCCGCCCGAAATCTAATGCCCCCATGatcat                                                                                                    >  1:470618/1‑146 (MQ=255)                                                                 ccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAAAAAGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCcat                                                                                    >  1:27748/1‑147 (MQ=255)                                                                  cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATTTTGAAAAAGCACGCCCGAAAACTAAAGGCCGCATTATCATTATTGAACCgcgccttaac                                                                                 >  1:1085640/1‑143 (MQ=255)                                                                      ggATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGGACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCCACAcc                                                                           >  2:261790/1‑151 (MQ=255)                                                                       gATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCCGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACAcc                                                                           >  2:716765/1‑150 (MQ=255)                                                                           tCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGGCCGCATGATCATCATCGAACCGCGCTATACCGACAcccggg                                                                       >  1:1111009/1‑146 (MQ=255)                                                                           tCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCCGAAAACTAATGGCCGCATGATCATCATCGCTCCCCGCTATACCGCCACCCGTg                                                                       >  1:911805/1‑150 (MQ=255)                                                                            cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAATCTAATGCCCCCATGATCATCATCGATCCGCGCTATACCGACACCGGTGcc                                                                     >  2:257153/1‑151 (MQ=255)                                                                                aaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgc                                                                 <  2:1267638/151‑1 (MQ=255)                                                                                  ctgTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCCGAAAACTAATTCCCGCATGATCATAATCGATCCGCGCTATACCCACACCGGGGCCggggg                                                                >  1:820606/3‑148 (MQ=255)                                                                                  cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAATAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcgc                                                               <  1:1238876/151‑1 (MQ=255)                                                                                   aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGcgcg                                                              <  1:1008341/151‑1 (MQ=255)                                                                                   aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGcgcg                                                              >  2:165652/1‑151 (MQ=255)                                                                                           ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGa                                                       <  1:680241/150‑1 (MQ=255)                                                                                            gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGAGGGGGGGGTTATTATCTTGAACAAGCACGCCCGGAAACTAATGCCCGCCTGATCATCATCGATCCGCGCTATAACGACACCGGTGGCGGGCGCGGAGATGAg                                                      >  2:129670/1‑150 (MQ=255)                                                                                                tGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTTTCTTGAAAAAGCACGCCAGAAAACTAATGCCCGCCTGGTCATCATCGATCCGCGCTATACCGACACCCGGGCCGGGCGCGGAGATGAGTgg                                                   >  2:1162785/1‑149 (MQ=255)                                                                                                 gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGCGTGACCTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATc                                                <  1:39579/151‑1 (MQ=255)                                                                                                     tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTATTATCTTTAACAAGCACGCCCGAAAACTAATGCCCCCATGATCATCCTCGATCCGCGCTATACCCACACCGGTGCCGGGCGCGGAGATGAggggggtcc                                              >  1:17491/1‑144 (MQ=255)                                                                                                           aataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGt                                       <  1:342503/150‑1 (MQ=255)                                                                                                            ataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTTTATCTTTAAAAGGGACGCCAGAAAAATAATGCCCGCCTGATCATCATCGATCCGCGCTATACCCACACCCGGGCCCGGCGCGGAGATGAGGGGGACCCCATTCGTcc                                     >  1:291535/1‑151 (MQ=255)                                                                                                              aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTTAACAGGCACGCCCGAAAAATAAAGCCCGCATGATCATCATCGATCCGCGCTATAACCACACCGGTGCCCGGGGCGGAGATGAGGGGATCCCCATTTGTcccg                                   >  1:549489/1‑149 (MQ=255)                                                                                                                 ccTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGaa                                <  2:1124353/151‑1 (MQ=255)                                                                                                                      cGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGAt                           <  2:97324/151‑1 (MQ=255)                                                                                                                          aCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCg                       <  1:1183681/151‑1 (MQ=255)                                                                                                                                aTGAGTGGCGGTGGGGGGACTTACTATCTTTAAAAAGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCCACACCCGTGGCGGGGGCGAAGATGAGTGGATCCCTATTTGTCCCGGGACAGATGCCGCACTgg                 >  1:920141/1‑151 (MQ=255)                                                                                                                                  gAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGt                <  2:619860/150‑1 (MQ=255)                                                                                                                                      ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc            <  2:85395/150‑1 (MQ=255)                                                                                                                                      ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg           <  1:445995/151‑1 (MQ=255)                                                                                                                                       gCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACgg          <  1:18506/151‑1 (MQ=255)                                                                                                                                          gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCt       >  2:68191/1‑151 (MQ=255)                                                                                                                                             gggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGc    <  1:1242940/151‑1 (MQ=255)                                                                                                                                             gggTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGc    <  1:1096163/151‑1 (MQ=255)                                                                                                                                               gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  <  2:190953/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                      CTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941570‑941862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑