Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,324	0	T	G	42.1%	136.4 / 17.1	76	G33G (GGT→GGG)	ynbB	putative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base T (8/36);  new base G (32/0);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 3.41e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTT  >  NC_000913/1478176‑1478474                                                                                                                                                      |                                                                                                                                                         aCTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGa                                                                                                                                                        <  1:488013/151‑1 (MQ=255) aCTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGa                                                                                                                                                        <  1:1244227/151‑1 (MQ=255)   tGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGAGG‑GAAAAAGGGGGGAgg                                                                                                                                                      >  1:521627/1‑151 (MQ=255)           aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAGGGGGGGAGGGTACAtt                                                                                                                                               >  2:523846/1‑150 (MQ=255)              gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAGGGGGGGAGGGTACCTTA‑c                                                                                                                                            >  2:133310/1‑149 (MQ=255)              gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGt                                                                                                                                          <  1:137222/151‑1 (MQ=255)               ttGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGGGA‑GAAAAGGGGGGGAggggttatttcgt                                                                                                                                           >  1:1061263/1‑139 (MQ=255)                 gTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTAt                                                                                                                                        <  1:258578/150‑1 (MQ=255)                    ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGGTTGCGGGGG‑GAAAAGGGGGGGAGGTTATAtttcacgttcgt                                                                                                                                     >  1:43641/1‑141 (MQ=255)                             ttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGAGA‑GAAAAGGGGGGGGGGGTACATTT‑CGGATTTGGACCttgggg                                                                                                                           >  2:259151/1‑146 (MQ=255)                                 tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTggg                                                                                                                          <  1:991705/148‑1 (MQ=255)                                     aGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGAGG‑GAAAAAGGGGGGAGGGTAAATTA‑CGTATTTGTTACTGGGGGGGGAt                                                                                                                      >  1:938419/1‑148 (MQ=255)                                         cTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAGGGGGGGGGGGTACATTA‑CTTATTTGTACCTGGGGGGGCGGCGTCCtt                                                                                                               >  2:128027/1‑151 (MQ=255)                                            aaaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGt                                                                                                            <  1:236323/151‑1 (MQ=255)                                             aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGAGA‑GAAAAGGGGGGGGGGGTACATTA‑CGTATTCGTACCTGGTGGGGGGTCGTCCtttttt                                                                                                           >  2:703199/1‑151 (MQ=255)                                                ataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGtttt                                                                                                         <  1:700096/150‑1 (MQ=255)                                                  aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGAGA‑GAAAAAGGGGGGGGGGTACATTA‑CGTATTTGTACCTGGGGGGGGGTCGTCATttttttttt                                                                                                       >  1:557286/1‑149 (MQ=255)                                                  aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAAGGGGGGGGGGTACATTA‑CGTATTCGTAACTGGGGGGGGATCGTCATTTGttttt                                                                                                        >  1:1011468/1‑149 (MQ=255)                                                     gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAAt                                                                                                    <  1:890278/150‑1 (MQ=255)                                                     gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATg                                                                                                   <  1:908282/151‑1 (MQ=255)                                                     gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATg                                                                                                   <  1:277620/151‑1 (MQ=255)                                                        ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAAGGGGGGGGGTTTTATTT‑TGTTTTTTTTCCTGGTGGGGGATCGTCATttttttttttat                                                                                                    >  1:865350/1‑143 (MQ=255)                                                        ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATgg                                                                                                  <  1:577854/149‑1 (MQ=255)                                                         gAAAAATCTCTTGCAACACTTTTCGCGTTGGTAATTTTAGCAAAGGTGATTAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGa                                                                                               <  2:119603/151‑1 (MQ=255)                                                         gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAAGGGGGGAGGGTACATTA‑CGTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTTAATGGTg                                                                                                >  2:743220/1‑150 (MQ=255)                                                          aaaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCTGGGC‑GCAAAGGGGGGGGGGGTACCATA‑TCTATTCTTAATTGGTGGGGGATTGTCATtttttttt                                                                                                        >  1:954782/1‑141 (MQ=255)                                                              aTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGGGG‑GAAAAAGGGGTGGGGGTTCATTA‑CGGATTTGTAACTGGTGGGGGATCGTCATTTGTTTTTTTATGGGGAgtt                                                                                            >  2:775922/1‑149 (MQ=255)                                                               tctcTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGG‑GAAAAAGGGGGGAGGGTAAATTT‑CGTATTCGTACCTGGGGGGGGATCCTCATTTTTTTTTTTATGGTGAttt                                                                                            >  2:1097583/1‑148 (MQ=255)                                                                 tctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGGTACAATA‑CGTATTCGTACCCGGGGGGGGATCGTCATTTGTTTTTTTATGGGGATTTTAgg                                                                                        >  2:216764/1‑150 (MQ=255)                                                                   tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAg                                                                                         <  1:61231/147‑1 (MQ=255)                                                                    ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTcc                                                                                     <  1:1204480/150‑1 (MQ=255)                                                                     gCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCAc                                                                                   <  1:812347/151‑1 (MQ=255)                                                                      cAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GGAAAAGGGGGGAGGGGACAATATCGAATT‑GTTCATGGTGGGGAAACGGTTTTTTTTTTTTTATGGTTATTTCAGGTTCAc                                                                                   >  2:1227576/1‑150 (MQ=255)                                                                       aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACGGAGAGGAAAAGGGGGGGGGGTT‑CATTT‑CTTATTCGTACCTGGTGGGGCCTCGGCATTTGTTTTTTAATGGGGATTTTAAGTCCCCg                                                                                  >  1:1178851/1‑150 (MQ=255)                                                                       aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGGTACATTT‑CGTATTTGTTCCTGGGGGGGGGTCGTCATTTTTTTTTTTATTGGGATTTTAGGGCCACG‑c                                                                                >  1:406695/1‑151 (MQ=255)                                                                        acacTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAAAGGGGGAGGGTACATTA‑CGTATTCGTACCTGGTGGGGGATCGTCATTTGTTTTTTAATTGTGGTTTCTGGTCCACG‑c                                                                                >  1:628887/1‑150 (MQ=255)                                                                          acTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTgg                                                                             <  1:1172954/151‑1 (MQ=255)                                                                                 gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGG‑GAAAAGGGGGGGAGGGTACATTT‑CGTATTTGTTCCTTGGGGGGGATCGTCATTTTTTTTTTTATGGGGGTTTTAGGTCCACG‑CTGGGtgacg                                                                       >  1:154204/1‑150 (MQ=255)                                                                                 gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGAtgacgt                                                                      <  1:672774/151‑1 (MQ=255)                                                                                 gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGAtgacgt                                                                      <  2:311722/151‑1 (MQ=255)                                                                                  cgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGAtgacgtt                                                                     <  1:1157985/151‑1 (MQ=255)                                                                                   gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAAGGGGGGGGGTTTAATTA‑AGTATTTGTACCTTGTGGGGGAGCGTCATTTTTTTTTTTATGGGGGTTTCTGGGCCACG‑CCGGGtgacgttg                                                                    >  2:93512/1‑151 (MQ=255)                                                                                          aTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGtttt                                                             <  1:1121309/151‑1 (MQ=255)                                                                                           ttttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGGGA‑GGAAAAGGGGGGAGGGTAAATTA‑CCTGTTCGTTCCCGGGGGGGCAGCGTCATTTGTTTTTTTATGGTGGTTTCAGGGTCAAG‑CTGGATGACGTTGGCGgtttt                                                            >  2:948320/1‑151 (MQ=255)                                                                                             ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGAGGGTTCATTT‑CGTATTTGTTACTGGGGGGGGATCGGCATTTTTTTTTTTATTGTGGTTTTAAGGCCACG‑CTGGGTGACGGTTTCGttttttt                                                          >  1:557861/1‑150 (MQ=255)                                                                                              tAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGG‑GAAAAGGGGGGGGGGGTACATTT‑CCTATTTGTACCTGGGGGGGCGTCGTCATTTTTTTTTTAAATGGGATTTTAGGTCCACG‑CTGGGTGACGGTGGCGtttttt                                                           >  2:380558/1‑149 (MQ=255)                                                                                                 cAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCt                                                      <  2:7338/151‑1 (MQ=255)                                                                                                    cGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGG‑GAAAAGGGGGGGGGGTTACATTA‑CGTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTTTATGGGGGTTTTAGGGCCACC‑CCGGGTGACGTTTGCGtttttt                                                           >  2:1051021/1‑143 (MQ=255)                                                                                                     gCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGA‑GAAAAGGGGGGGGGGGTACATTA‑CGTATTTGTTCCTTGTGGGGGGTCGTCCTTTTTTTTTTTATTGTGGTTTTAGGGCCACGCCGGGTTGAGGTTG‑CGTTTTTTTTGCTgtt                                                   >  1:643975/1‑148 (MQ=255)                                                                                                     gCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGG‑GAAAAAGGGGGGGGGGTACATTA‑CGTATTTGTACCTTGTGGGGGATCGTCATTTTTTTTTTTATGGGGGTTTTTGGGCCACG‑CCGGGTGACGTTGACGgttttt                                                           >  1:1237302/1‑142 (MQ=255)                                                                                                     gCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GGAAAAGGGGGGAGGGTACATTT‑CGTATTTGTACCTGGGGGGGGATCGTCATTTGTTTTTTTATGGTGATTTTAGGTCCACG‑CTGGGTGACGTTGACGTTTTTTTCGCTGAt                                                   >  2:1196737/1‑150 (MQ=255)                                                                                                       tGATAAACCGTTTTCTGCTATGGCGGTTTTCCGGGA‑GCAAAGGGGGGGTGGTTACATTT‑AGTATTTGTACCTTGTGGGGCCTCGTCATTTTTTTTTTTATGGGGATTTTTTGGCCCCG‑CTGGGGGACGGTTTCGTTTTTTTCGCTGAt                                                   >  1:904345/1‑148 (MQ=255)                                                                                                       tGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGA‑GAAAAGGGGGGGGGGGTACATTA‑CGTATTTGTACCTGGTGGGGGATCGGCATTTGTTTTTTAATGGGGATTTTAGGGCCACG‑CTGGGTGACGTTTACGTTTTTTTCGCTggt                                                   >  2:496709/1‑146 (MQ=255)                                                                                                        gATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGt                                               <  1:683631/151‑1 (MQ=255)                                                                                                        gATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGt                                               <  2:1061283/151‑1 (MQ=255)                                                                                                              ccGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTg                                         <  1:777938/151‑1 (MQ=255)                                                                                                               cGTTTTCTGCTATGGCGGTTATCGGGGA‑GAAAAGGGGGGGGGGGTACATTA‑CGGAATCGTACCTGGTGGGGGAACGTCATTTGTTTTTTTATGGGGGTTTTCGGGCCCCG‑CTGGGTGACGTTGACGGTTTTTTTGCTGATAAGtttttttg                                        >  2:1008984/1‑147 (MQ=255)                                                                                                                       gCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGaaa                                <  2:521635/151‑1 (MQ=255)                                                                                                                          aTGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAg                               <  1:976261/149‑1 (MQ=255)                                                                                                                            ggCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat                            <  2:723073/150‑1 (MQ=255)                                                                                                                            ggCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat                            <  2:1069803/150‑1 (MQ=255)                                                                                                                             gCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAatat                          <  1:635289/151‑1 (MQ=255)                                                                                                                                  tACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc                     >  2:1010673/1‑151 (MQ=255)                                                                                                                                   aCCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc                     <  1:1145827/150‑1 (MQ=255)                                                                                                                                   aCCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                    >  1:39496/1‑151 (MQ=255)                                                                                                                                   aCCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                    >  1:350228/1‑151 (MQ=255)                                                                                                                                     cGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCt                  <  2:514122/151‑1 (MQ=255)                                                                                                                                        aga‑gaAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat               <  1:121110/151‑1 (MQ=255)                                                                                                                                            gaAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATc            <  1:1039589/151‑1 (MQ=255)                                                                                                                                             aaaaGGGGGTGCGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt           >  2:546433/1‑151 (MQ=255)                                                                                                                                              aaaGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg          <  1:523838/151‑1 (MQ=255)                                                                                                                                               aaGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt         >  1:794536/1‑151 (MQ=255)                                                                                                                                                aGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTa        <  2:54428/151‑1 (MQ=255)                                                                                                                                                   gggTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAt     <  1:133306/151‑1 (MQ=255)                                                                                                                                                   gggTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAt     >  1:182938/1‑151 (MQ=255)                                                                                                                                                   gggTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAt     >  2:145716/1‑151 (MQ=255)                                                                                                                                                      tGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAtttt  >  1:921584/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                         ACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGA‑GAAAAGGGGGTGAGGTTACATTA‑CGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACG‑CTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTT  >  NC_000913/1478176‑1478474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑