Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,268	0	T	A	42.7%	84.3 / 41.9	75	L24F (TTA→TTT)	emrK	multidrug resistance efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base T (24/19);  new base A (0/32);  total (24/51)
Fisher's exact test for biased strand distribution p-value = 3.34e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483120‑2483416                                                                                                                                                       |                                                                                                                                                     tCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑Caataaata                                                                                                                                                     <  2:423780/150‑1 (MQ=255)   gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATaaaa                                                                                                                                                  <  1:980437/151‑1 (MQ=255)       cTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTAc                                                                                                                                              >  1:1106626/1‑151 (MQ=255)         ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTAcc                                                                                                                                             <  2:1147850/150‑1 (MQ=255)             gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCaa                                                                                                                                        <  2:50599/151‑1 (MQ=255)             gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCa                                                                                                                                         <  1:811795/150‑1 (MQ=255)                           acacTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTAtt                                                                                                                          >  1:874378/1‑151 (MQ=255)                             acTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTAtttt                                                                                                                        >  1:442730/1‑151 (MQ=255)                              ctcccTGATATTTGTGCAAAATTTGAATCTGCATTCCCCGTGACATAGGCCTCATTTTTACTAATCATGTTTTCTAATTCCATTGC‑CAAATAGCCATAGGCCCCTAAAACCG‑CAAAAAATAAAACTACCGCCAATAAAGAAAAGTAttt                                                                                                                         <  2:610745/146‑1 (MQ=255)                                aCCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCATGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                                     >  1:459393/1‑151 (MQ=255)                                 ccTGAGATTTGTGAAGAAATTGGTTCTGCATCCCCCGTAACATAGGCGTCATCTGTACAAATCATGTCTTCAAATCCCATTAA‑CAAAAAGAAATAGGCACCTAAAAACA‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGt                                                                                                                             <  1:1175333/142‑1 (MQ=255)                                 ccTGAGACTTGTGCAAAAATTGGTTTTGCCTCCCCCGTGCAATGGGCGTCCTCTGAACAACTCTTTTCTCCTAATCCCTTAAA‑CCAATAGGCAGAGGCCCCT‑AAAACGTCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTttcttc                                                                                                                    <  2:130808/151‑1 (MQ=255)                                 ccTGAGACTTGTGAAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCCCCTGAAAACG‑AAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                                                                                                                     <  2:276913/150‑1 (MQ=255)                                  cTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTtcttct                                                                                                                   >  1:657699/1‑151 (MQ=255)                                   tGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTg                                                                                                                  >  2:1251534/1‑151 (MQ=255)                                   tGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTg                                                                                                                  >  1:805686/1‑151 (MQ=255)                                    gagtCTTGTGCAAAAATTGGATCTCCATCCCCCGTAACAAAGGCCTCTTCTGTACTAACCATTTCTTAAAATCCCATTGA‑CAAATAGGCATAGGCACCTGAAAACG‑AAAAAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGt                                                                                                                 <  1:113501/151‑1 (MQ=255)                                     agaCTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGtt                                                                                                                >  1:384086/1‑151 (MQ=255)                                         ttGGGCAAAAATTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGA‑CAAATAGGCAAAGGCCCCTAAAAACG‑CAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg                                                                                                              <  1:169838/149‑1 (MQ=255)                                               aaaaaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttt                                                                                                       <  2:571463/148‑1 (MQ=255)                                                 aaaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTTTTCTAATCCCATTGC‑CAAATAGGCATAGGCCCCTAAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt                                                                                                     <  2:72470/150‑1 (MQ=255)                                                  aaTTGGATCTGCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                   <  2:657708/151‑1 (MQ=255)                                                  aaTTGGATCTCCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGA‑CAAATAGGAATAGACACTTAAAAACA‑AAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa                                                                                                   <  1:20987/151‑1 (MQ=255)                                                            gCATCCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CAAATAGGCATAGGCACCTAAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa                                                                                          <  2:15911/150‑1 (MQ=255)                                                               ttCCCCGTGACATGGGCGTCATCTGTACTAATCATTTTTTCTAATTCCATTGA‑CCAAAAGGAATAGGCACTTGAAAACG‑AAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa                                                                                              <  1:1047801/143‑1 (MQ=255)                                                               ttCCCCGTGACATAGGCGTCATCTGTACAAATCATGTCTTCTAATTCCATTGC‑CCAAAAGGCATAGGCACCTGAAAACG‑AAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt                                                                                            <  1:531136/145‑1 (MQ=255)                                                               tcccccGTAACATAGGCTTCATCTTTACTAATAATGTTTTCTAATTCCATTGA‑CCAAAAGGAAAAGGCACCTAAAAACG‑AAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt                                                                                           <  1:522473/144‑1 (MQ=255)                                                                 ccccGTAACATGGGCGTCATCTTTACTAATAATGTCTTATAATTCCATTGA‑CCAATAGGAATAGCCCCCTAAAAACG‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGt                                                                                    <  1:430097/151‑1 (MQ=255)                                                                  cccGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGtt                                                                                   >  1:311790/1‑151 (MQ=255)                                                                      tGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCCCCTGAAAACG‑CAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                <  2:826881/150‑1 (MQ=255)                                                                       gACATAGGCGTCATCTGTACAAATCATTTCTTCTAATCCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                <  2:1144825/149‑1 (MQ=255)                                                                        aCATGGGCGTCATCTGTACAATTAATTTCTTCT‑ATTTCATTAACCCAAAAGCCATAACCCCATAAAACTA‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa                                                                                <  1:641086/148‑1 (MQ=255)                                                                        aCATAGGCGTCATCTGTACTAATCTTGTTTTCTAATTCCATTGA‑CAAATAGGCATAGCCACCGGAAAACG‑AAAAAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt                                                                              <  2:166156/150‑1 (MQ=255)                                                                             ggCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATGGGCATAGCCACCTAAAACCG‑CAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtc                                                                        <  2:375471/151‑1 (MQ=255)                                                                              gCGTCATCTGTACTAATCATGTTTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑AAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtct                                                                       <  1:785620/151‑1 (MQ=255)                                                                               cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctc                                                                      >  2:580239/1‑151 (MQ=255)                                                                               cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTAtctc                                                                      >  1:1036256/1‑151 (MQ=255)                                                                                    tCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAtt                                                                 >  1:1175759/1‑151 (MQ=255)                                                                                      tGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                                               >  2:257746/1‑151 (MQ=255)                                                                                          cTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                           >  2:238122/1‑151 (MQ=255)                                                                                            aaccATGTCTTCTAATCCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑AAATAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCa                                                            <  2:83713/145‑1 (MQ=255)                                                                                            aaTCATTTCTTCTAATCCCATTGA‑CCAATAGGCATAGGCACCTAAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc                                                               <  1:735418/145‑1 (MQ=255)                                                                                            aaTCATGTCTTCTAATCCCATTAA‑CCAATGGGCATAGGCACCTAAAAACG‑CAATAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAg                                                         <  2:413588/151‑1 (MQ=255)                                                                                             aTCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCAt                                                           >  2:457983/1‑148 (MQ=255)                                                                                              taatctcttcaAATTCCATTGA‑CAAAAAGGAATAGGCCCTTGAAATCG‑AAAAAAAAAAACTTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGa                                                        <  1:757434/145‑1 (MQ=255)                                                                                               cATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                      >  1:778334/1‑151 (MQ=255)                                                                                                 tGTCTTCAAATTCCATTGA‑CCAATAGGCATAGGCACCTAAAAACG‑AAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                       <  1:1063636/148‑1 (MQ=255)                                                                                                  gTCTTCTAATTCCATTGA‑CAAATAGGAAAAGGCACCTAAAAACG‑AAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg                                                      <  1:83791/148‑1 (MQ=255)                                                                                                     ttctAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAttt                                                >  1:1208514/1‑151 (MQ=255)                                                                                                     ttctAATCCCATTGC‑CAAAAAGGCAGGGCCACCAAAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGAt                                                       <  2:1203701/144‑1 (MQ=255)                                                                                                      tttAATCCCATCGA‑CAAAGGGGAATAGGCACTAAAAACCG‑CAATAAATAAATTTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAAtt                                                 <  1:402945/147‑1 (MQ=255)                                                                                                         atttCCATTGA‑CCAAAAGGCATAGGCACCTGAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtga                                            <  2:733607/149‑1 (MQ=255)                                                                                                         aaTTCCATTGC‑CAAATAGGAATAGGCCCCTAAAAACG‑AAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTa                                               <  2:167/148‑1 (MQ=255)                                                                                                         aaTTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtg                                             >  1:953666/1‑150 (MQ=255)                                                                                                         aaTTCCATTGA‑CCAATAGGAAAAGGCACCTAAAAACG‑AAATAAATAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTa                                               <  1:216015/148‑1 (MQ=255)                                                                                                           ttCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaat                                          >  1:42658/1‑151 (MQ=255)                                                                                                             ccATTGA‑CAAATAGGCATAGGCCCCTAAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaatt                                         <  2:570583/150‑1 (MQ=255)                                                                                                               aTTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattga                                       >  2:1026571/1‑150 (MQ=255)                                                                                                                 tGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatg                                    >  2:859962/1‑151 (MQ=255)                                                                                                                   a‑CCAATAGGCATAGGCACCAGAAACCG‑CAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat                                     <  2:341461/148‑1 (MQ=255)                                                                                                                     ccAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatgaa                                  >  1:496698/1‑150 (MQ=255)                                                                                                                     ccAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                                   <  2:901917/149‑1 (MQ=255)                                                                                                                      cAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaa                                 >  1:912605/1‑150 (MQ=255)                                                                                                                       aataggaataggCCCCTAAAAACA‑CAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaaa                                <  1:553638/150‑1 (MQ=255)                                                                                                                       aaaaggaataggcaCCTGAAAACG‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGaaaa                                <  1:331462/147‑1 (MQ=255)                                                                                                                       aaTAGGCATAGGCACCTGAAAACG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatga                                   <  1:733310/147‑1 (MQ=255)                                                                                                                       aaTAGCCATAGGCACCTAAAACCG‑AAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatgaa                                  <  2:996326/148‑1 (MQ=255)                                                                                                                           ggaaaaggcaCCTAAAAACA‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAagag                             <  1:1200358/144‑1 (MQ=255)                                                                                                                                aggcaCCTAAAAACG‑CAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGca                           <  2:442045/146‑1 (MQ=255)                                                                                                                                aggcaCCTAAAAACG‑AAATAAAAAAAATTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcat                      <  1:438183/151‑1 (MQ=255)                                                                                                                                aggcaCCTAAAAACG‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattca                       <  1:562047/150‑1 (MQ=255)                                                                                                                                 ggcaCCTAAAAACG‑AAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattcat                      <  2:385010/150‑1 (MQ=255)                                                                                                                                 ggcaCCTAAAAACG‑AAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcat                          <  1:472834/146‑1 (MQ=255)                                                                                                                                         aaatCG‑AAAAAAATAAAATTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCa                <  1:471545/148‑1 (MQ=255)                                                                                                                                         aaaaCG‑CAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCa                <  2:1106646/148‑1 (MQ=255)                                                                                                                                          aaaCG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATc            >  1:778343/1‑151 (MQ=255)                                                                                                                                                 aataaataAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAac        <  1:128698/149‑1 (MQ=255)                                                                                                                                                  ataaataaAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAAcaca     <  2:893335/151‑1 (MQ=255)                                                                                                                                                   taaataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac      >  2:1220101/1‑149 (MQ=255)                                                                                                                                                     aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt   >  2:237622/1‑150 (MQ=255)                                                                                                                                                     aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTg  <  1:925727/151‑1 (MQ=255)                                                                                                                                                     aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTg  <  1:453493/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                     TCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGA‑CCAATAGGCATAGGCACCTGAAAACG‑CAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG  >  NC_000913/2483120‑2483416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑