Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	99,891	C→T	A83V (GCC→GTC)	murG →	N‑acetylglucosaminyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	99,891	0	C	T	100.0%	360.2 / NA	91	A83V (GCC→GTC)	murG	N‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/49);  total (42/49)

GGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCT  >  NC_000913/99744‑100037                                                                                                                                                    |                                                                                                                                                   ggCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCt                                                                                                                                                  <  1:1149159/150‑1 (MQ=255) ggCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTg                                                                                                                                                 >  2:118818/1‑151 (MQ=255)        tCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGt                                                                                                                                             >  2:197530/1‑148 (MQ=255)        tCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTc                                                                                                                                            >  1:815733/1‑149 (MQ=255)         cGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAgg                                                                                                                                         <  2:643871/151‑1 (MQ=255)              gctggGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCgg                                                                                                                                    <  1:1097026/151‑1 (MQ=255)              gctggGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCgg                                                                                                                                    >  1:302074/1‑151 (MQ=255)                  gggACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGa                                                                                                                                <  2:122502/151‑1 (MQ=255)                   ggACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGAt                                                                                                                               >  1:64701/1‑151 (MQ=255)                   ggACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGAt                                                                                                                               <  1:53937/151‑1 (MQ=255)                    gACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGAtt                                                                                                                              <  1:960992/151‑1 (MQ=255)                         ccGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         <  2:659906/151‑1 (MQ=255)                          cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         >  1:1114133/1‑150 (MQ=255)                          cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         >  1:305287/1‑150 (MQ=255)                           gACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAg                                                                                                                       <  1:310778/151‑1 (MQ=255)                            aCCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGc                                                                                                                      <  2:98236/151‑1 (MQ=255)                              cGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGt                                                                                                                    <  2:938688/151‑1 (MQ=255)                               gTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTTGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTa                                                                                                                   <  2:707708/151‑1 (MQ=255)                                 aTGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCTCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACa                                                                                                                 <  1:129341/151‑1 (MQ=255)                                   ggAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACaaa                                                                                                               <  1:2683/151‑1 (MQ=255)                                   ggAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACaa                                                                                                                <  1:1135453/150‑1 (MQ=255)                                     aaGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAAcc                                                                                                             <  2:1096901/151‑1 (MQ=255)                                      aGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCt                                                                                                            >  1:817106/1‑151 (MQ=255)                                        cGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGa                                                                                                          <  1:850899/151‑1 (MQ=255)                                         ggACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGAc                                                                                                         <  2:578083/151‑1 (MQ=255)                                               aGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACgtggtg                                                                                                   >  1:459899/1‑151 (MQ=255)                                                    cAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCgg                                                                                              >  1:487255/1‑151 (MQ=255)                                                     aaaaCATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGt                                                                                             >  1:463139/1‑151 (MQ=255)                                                     aaaaCATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGt                                                                                             <  2:922935/151‑1 (MQ=255)                                                            ggCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGa                                                                                       <  2:167731/150‑1 (MQ=255)                                                              cATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGc                                                                                    >  2:183322/1‑151 (MQ=255)                                                                tCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCt                                                                                   >  2:446734/1‑150 (MQ=255)                                                                 cGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTa                                                                                  <  1:720460/150‑1 (MQ=255)                                                                 cGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTAc                                                                                 <  1:553999/151‑1 (MQ=255)                                                                   aaaTTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACgt                                                                               >  2:483314/1‑151 (MQ=255)                                                                   aaaTTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACgt                                                                               >  2:165964/1‑151 (MQ=255)                                                                    aaTTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACgtg                                                                              >  1:208394/1‑151 (MQ=255)                                                                       tGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTc                                                                            >  1:119921/1‑150 (MQ=255)                                                                           ttCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTc                                                                       >  1:537647/1‑151 (MQ=255)                                                                            tCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTcc                                                                      >  1:978461/1‑151 (MQ=255)                                                                             cATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCa                                                                     <  1:1114430/151‑1 (MQ=255)                                                                             cATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCa                                                                     <  1:603583/151‑1 (MQ=255)                                                                              aTTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTcc                                                                      >  1:1269216/1‑149 (MQ=255)                                                                              aTTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTcc                                                                      >  2:517074/1‑149 (MQ=255)                                                                               ttCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGGAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAgg                                                                   >  2:1032125/1‑151 (MQ=255)                                                                                 cGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACCAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggt                                                                  >  1:1224537/1‑150 (MQ=255)                                                                                  gTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggtgg                                                                >  2:191264/1‑151 (MQ=255)                                                                                        ctGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGc                                                          >  1:445551/1‑151 (MQ=255)                                                                                         tGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGcc                                                         >  2:906701/1‑151 (MQ=255)                                                                                          ggTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGcc                                                         <  1:234967/150‑1 (MQ=255)                                                                                               gCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCcgcg                                                      <  2:333484/148‑1 (MQ=255)                                                                                               gCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTgg                                                   <  2:785080/151‑1 (MQ=255)                                                                                                cGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTgg                                                   >  2:176082/1‑150 (MQ=255)                                                                                                cGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGt                                                  <  2:926715/151‑1 (MQ=255)                                                                                                 gTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTc                                                 <  1:55634/151‑1 (MQ=255)                                                                                                  tGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCg                                                >  1:1162208/1‑151 (MQ=255)                                                                                                     aaaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGtt                                              >  2:517135/1‑150 (MQ=255)                                                                                                           tataAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCAtt                                       >  1:695824/1‑151 (MQ=255)                                                                                                             taAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTcc                                     >  1:744971/1‑151 (MQ=255)                                                                                                              aaaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTcc                                     <  2:335317/150‑1 (MQ=255)                                                                                                                 aGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGtt                                 >  2:913874/1‑151 (MQ=255)                                                                                                                 aGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGtt                                 <  2:643740/151‑1 (MQ=255)                                                                                                                  gCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTg                                <  1:536225/151‑1 (MQ=255)                                                                                                                   cACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGt                               <  1:70254/151‑1 (MQ=255)                                                                                                                   cACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGt                               <  2:815745/151‑1 (MQ=255)                                                                                                                    aCTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGt                               >  2:695959/1‑150 (MQ=255)                                                                                                                       gATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACtt                           <  1:1185579/151‑1 (MQ=255)                                                                                                                       gATAGCTGCCCCGCTGCGTATCTTCAACGTCCGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACtt                           >  2:430537/1‑151 (MQ=255)                                                                                                                          aGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCAt                        <  2:388851/151‑1 (MQ=255)                                                                                                                           gCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATg                       >  2:763743/1‑151 (MQ=255)                                                                                                                            cTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATg                       >  1:542001/1‑150 (MQ=255)                                                                                                                              gCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAAc                    >  2:42384/1‑151 (MQ=255)                                                                                                                              gCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAAc                    >  2:835274/1‑151 (MQ=255)                                                                                                                               ccccGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAAc                    >  1:1248643/1‑150 (MQ=255)                                                                                                                               ccccGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACa                   <  1:160975/151‑1 (MQ=255)                                                                                                                                cccGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACaa                  <  2:819225/151‑1 (MQ=255)                                                                                                                                cccGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACaa                  <  2:302078/151‑1 (MQ=255)                                                                                                                                   gCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAAc               <  1:143301/151‑1 (MQ=255)                                                                                                                                   gCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAAc               <  1:165960/151‑1 (MQ=255)                                                                                                                                      gCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGt            <  2:1114310/151‑1 (MQ=255)                                                                                                                                      gCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGt            >  1:454389/1‑151 (MQ=255)                                                                                                                                      gCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGt            <  2:511231/151‑1 (MQ=255)                                                                                                                                       cGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTa           <  2:61496/151‑1 (MQ=255)                                                                                                                                        gTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTAt          >  2:4429/1‑151 (MQ=255)                                                                                                                                          aTCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTAtt         >  1:660911/1‑150 (MQ=255)                                                                                                                                          aTCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTAtt         <  1:132445/150‑1 (MQ=255)                                                                                                                                              tCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTg        <  2:305291/147‑1 (MQ=255)                                                                                                                                              tCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTg        <  2:1114153/147‑1 (MQ=255)                                                                                                                                              tCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTg        <  2:445146/147‑1 (MQ=255)                                                                                                                                               cAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCggg    <  2:1005675/150‑1 (MQ=255)                                                                                                                                                aaCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCt  <  1:900321/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCT  >  NC_000913/99744‑100037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑