Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,923 C→T G36D (GGC→GAC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,9230CT99.1% 441.0 / NA 116G36D (GGC→GAC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (58/57);  minor base A (1/0);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

GTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAA  >  NC_000913/3440778‑3441068
                                                                                                                                                 |                                                                                                                                                 
gTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCaa                                                                                                                                                >  2:457338/1‑149 (MQ=255)
 ttGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGc                                                                                                                                             >  2:359062/1‑151 (MQ=255)
   gAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGcc                                                                                                                                            >  2:1100881/1‑150 (MQ=255)
    aGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCAc                                                                                                                                          <  1:198651/151‑1 (MQ=255)
      cAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGc                                                                                                                                        >  1:744262/1‑151 (MQ=255)
        ggATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctc                                                                                                                                      >  2:619301/1‑151 (MQ=255)
         gATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGctct                                                                                                                                     <  2:1022941/151‑1 (MQ=255)
              ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAgg                                                                                                                                >  2:114722/1‑151 (MQ=255)
              ccAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAg                                                                                                                                 <  1:1066999/150‑1 (MQ=255)
                aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCt                                                                                                                              <  2:278255/151‑1 (MQ=255)
                aGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCt                                                                                                                              >  1:360964/1‑151 (MQ=255)
                   atatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCaa                                                                                                                           <  1:979125/151‑1 (MQ=255)
                    tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCa                                                                                                                            >  2:896129/1‑149 (MQ=255)
                    tatCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCa                                                                                                                            >  2:740268/1‑149 (MQ=255)
                     atCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAgg                                                                                                                         >  2:966677/1‑151 (MQ=255)
                      tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAggg                                                                                                                        >  1:75308/1‑151 (MQ=255)
                      tCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAggg                                                                                                                        >  2:58735/1‑151 (MQ=255)
                              aaCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTg                                                                                                                <  2:770107/151‑1 (MQ=255)
                               aCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTgg                                                                                                               >  1:795667/1‑151 (MQ=255)
                               aCGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTgg                                                                                                               >  2:401198/1‑151 (MQ=255)
                                cGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgc                                                                                                              <  1:432032/151‑1 (MQ=255)
                                 gCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcg                                                                                                             <  1:187976/151‑1 (MQ=255)
                                  ccTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgt                                                                                                            <  2:475536/151‑1 (MQ=255)
                                   cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtg                                                                                                           >  2:359897/1‑151 (MQ=255)
                                   cttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtg                                                                                                           >  1:529729/1‑151 (MQ=255)
                                    ttcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgc                                                                                                          >  2:168845/1‑151 (MQ=255)
                                     tcttTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGgcgtgc                                                                                                          <  2:1034508/150‑1 (MQ=255)
                                       tttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTc                                                                                                       >  2:179639/1‑151 (MQ=255)
                                       tttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTc                                                                                                       >  2:262815/1‑151 (MQ=255)
                                        ttGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCg                                                                                                      >  2:5681/1‑151 (MQ=255)
                                          ggTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGa                                                                                                     <  2:83318/150‑1 (MQ=255)
                                             gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTc                                                                                                 >  1:1256220/1‑151 (MQ=255)
                                                gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACt                                                                                              <  2:877614/151‑1 (MQ=255)
                                                   cTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGCATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGAGCGTCGAACTCACTTGc                                                                                           >  1:1208705/1‑151 (MQ=255)
                                                   cTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                           >  1:813859/1‑151 (MQ=255)
                                                   cTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGc                                                                                           >  2:936574/1‑151 (MQ=255)
                                                     cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                          <  1:575229/150‑1 (MQ=255)
                                                     cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                         <  2:841850/151‑1 (MQ=255)
                                                     cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                         >  1:156841/1‑151 (MQ=255)
                                                      aTGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                        >  1:755154/1‑151 (MQ=255)
                                                       tGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                        >  1:1054239/1‑150 (MQ=255)
                                                        gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                      >  1:1236820/1‑151 (MQ=255)
                                                        gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGat                                                                                      >  2:656518/1‑151 (MQ=255)
                                                          agtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                    >  1:459513/1‑151 (MQ=255)
                                                          agtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACAGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                    >  2:117985/1‑151 (MQ=255)
                                                          aggccacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                    >  1:385150/5‑151 (MQ=255)
                                                           ataCACCATCAATCTCAACCTCGGTCACCGCGCAAACAGGCATCGATGAGAGCAGAATACGGCGCACTGCGTTACCCAGAGTATCGACAAAGCCACGCTCTAACGGCTCACGGGTCCCCTTGGCGGCCGTCGAACTCACTTGCTCGatat                                                                                    >  1:1057041/2‑150 (MQ=255)
                                                             acacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCa                                                                                  >  1:828229/1‑150 (MQ=255)
                                                                ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                              <  2:804928/151‑1 (MQ=255)
                                                                ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCa                                                                              <  1:52646/151‑1 (MQ=255)
                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                            <  2:444406/151‑1 (MQ=255)
                                                                  aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                            >  1:519950/1‑151 (MQ=255)
                                                                    cAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgc                                                                           <  2:82594/150‑1 (MQ=255)
                                                                         tcAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtt                                                                     >  1:284523/1‑151 (MQ=255)
                                                                          cAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGcgcg                                                                        <  1:589742/147‑1 (MQ=255)
                                                                          cAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGttt                                                                    <  2:1223244/151‑1 (MQ=255)
                                                                                 ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAc                                                             <  1:584036/151‑1 (MQ=255)
                                                                                 ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAAc                                                             <  2:666025/151‑1 (MQ=255)
                                                                                    cACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                          <  2:314457/151‑1 (MQ=255)
                                                                                     aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActct                                                          >  1:1041077/1‑150 (MQ=255)
                                                                                     aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                         >  1:391020/1‑151 (MQ=255)
                                                                                     aCCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                         >  1:245720/1‑151 (MQ=255)
                                                                                       cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTc                                                       <  1:350207/151‑1 (MQ=255)
                                                                                         cgcAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcac                                                     <  2:521298/151‑1 (MQ=255)
                                                                                            aaCCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                  <  2:538477/151‑1 (MQ=255)
                                                                                              cccGGCATCGTTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                    >  2:116480/1‑147 (MQ=255)
                                                                                               ccGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAcc                                               >  2:54310/1‑151 (MQ=255)
                                                                                                cGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAAccc                                              <  2:360969/151‑1 (MQ=255)
                                                                                                 ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                             <  1:552215/151‑1 (MQ=255)
                                                                                                 ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                             <  1:896115/151‑1 (MQ=255)
                                                                                                 ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCt                                             >  2:118186/1‑151 (MQ=255)
                                                                                                  gCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTg                                            >  1:779837/1‑151 (MQ=255)
                                                                                                    aTCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                          <  1:1076906/151‑1 (MQ=255)
                                                                                                      cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAtt                                        <  2:1129061/151‑1 (MQ=255)
                                                                                                      cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCAt                                         <  2:64562/150‑1 (MQ=255)
                                                                                                       gATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATtg                                       <  1:1223091/151‑1 (MQ=255)
                                                                                                            gagCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTcc                                  <  1:1176197/151‑1 (MQ=255)
                                                                                                             agCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                 >  2:782513/1‑151 (MQ=255)
                                                                                                              gCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAACCTCTGTCACAGAACCCTGCATTGTGTCct                                 <  2:1086031/150‑1 (MQ=255)
                                                                                                                aGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                              <  1:1126903/151‑1 (MQ=255)
                                                                                                                aGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                              <  2:289182/151‑1 (MQ=255)
                                                                                                                aGAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                              >  2:946217/1‑151 (MQ=255)
                                                                                                                 gAATACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctct                             <  1:677627/151‑1 (MQ=255)
                                                                                                                   aTACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                           <  2:400298/151‑1 (MQ=255)
                                                                                                                   aTACGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                           >  2:381650/1‑151 (MQ=255)
                                                                                                                     aCGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttt                           <  1:262811/149‑1 (MQ=255)
                                                                                                                     aCGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                          >  2:1163802/1‑150 (MQ=255)
                                                                                                                      cGGCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                        >  2:517202/1‑151 (MQ=255)
                                                                                                                       ggCGCAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGt                        >  1:318142/1‑150 (MQ=255)
                                                                                                                          gcAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt                     >  1:1225698/1‑150 (MQ=255)
                                                                                                                          gcAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTa                    <  1:1037245/151‑1 (MQ=255)
                                                                                                                           cAGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTaa                   <  2:103698/151‑1 (MQ=255)
                                                                                                                            aGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTa                    >  1:1169272/1‑149 (MQ=255)
                                                                                                                            aGTGCGTTACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAg                  <  2:853693/151‑1 (MQ=255)
                                                                                                                                   tACCCAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt           >  2:253423/1‑151 (MQ=255)
                                                                                                                                      ccAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGCTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg        >  1:893058/1‑151 (MQ=255)
                                                                                                                                       cAGAGTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGa       <  2:578289/151‑1 (MQ=255)
                                                                                                                                        agagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTgg        >  2:862136/1‑149 (MQ=255)
                                                                                                                                         gagTATGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGt     <  2:204987/151‑1 (MQ=255)
                                                                                                                                             aTGGTCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTaaa  <  2:141504/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GTTGAGCAGGATTTCCAGGATATCTTCCTGAACGCCTTCTTTGGTGCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACGCTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAA  >  NC_000913/3440778‑3441068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: