Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,025,248 +TC intergenic (+35/‑263) yodD → / → yedP uncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,025,2461.T94.4% 207.9 / 2.7 54intergenic (+33/‑265)yodD/yedPuncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base T (18/33);  total (20/34)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,025,2462.C94.4% 202.4 / 2.6 54intergenic (+33/‑265)yodD/yedPuncharacterized protein/putative mannosyl‑3‑phosphoglycerate phosphatase
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base C (18/33);  total (20/34)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑TCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGA  >  NC_000913/2025096‑2025385
                                                                                                                                                       ||                                                                                                                                           
ccAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAggggg                                                                                                                                               >  1:1110398/1‑151 (MQ=255)
 cAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑t                                                                                                                                            >  1:740086/1‑151 (MQ=255)
  aGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tc                                                                                                                                           >  1:313637/1‑151 (MQ=255)
       gATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcggg                                                                                                                                      >  1:1054378/1‑146 (MQ=255)
             gAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑Tctcgggatg                                                                                                                                   <  2:740092/148‑9 (MQ=255)
                  cGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGc                                                                                                                             <  1:317727/151‑1 (MQ=255)
                       gCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAg                                                                                                                        <  1:502435/151‑1 (MQ=255)
                        cGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGc                                                                                                                       <  1:1047584/151‑1 (MQ=255)
                        cGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGc                                                                                                                       >  2:333476/1‑151 (MQ=255)
                         gTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGc                                                                                                                       >  1:363607/1‑150 (MQ=255)
                            gATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCa                                                                                                                      >  2:1162296/1‑148 (MQ=255)
                                gACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCa                                                                                                               <  2:35299/151‑1 (MQ=255)
                                  cGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACt                                                                                                             <  2:1174989/151‑1 (MQ=255)
                                     gAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTaa                                                                                                          <  2:117004/151‑1 (MQ=255)
                                     gAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTa                                                                                                           >  2:1231160/1‑150 (MQ=255)
                                            aTACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTc                                                                                                   <  1:70487/151‑1 (MQ=255)
                                              aCATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCATTTAAAAAATTCg                                                                                                  <  1:662969/150‑1 (MQ=255)
                                              aCATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGt                                                                                                 <  1:1004181/151‑1 (MQ=255)
                                               cATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGtt                                                                                                >  1:38745/1‑151 (MQ=255)
                                               cATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGtt                                                                                                <  1:1117715/151‑1 (MQ=255)
                                                  ggCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTacg                                                                                             <  2:984937/151‑2 (MQ=255)
                                                    cGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACAcc                                                                                           >  2:790915/1‑151 (MQ=255)
                                                        aaTTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGa                                                                                       <  1:586515/151‑1 (MQ=255)
                                                              cGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTg                                                                                 <  2:1150307/151‑1 (MQ=255)
                                                                          aaCTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTtcatc                                                                      >  2:242517/1‑150 (MQ=255)
                                                                           aCTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCAc                                                                    >  2:1070623/1‑151 (MQ=255)
                                                                           aCTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCAc                                                                    <  1:26914/151‑1 (MQ=255)
                                                                              ggATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCtt                                                                 >  2:204110/1‑151 (MQ=255)
                                                                                     cATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGc                                                           >  1:521123/1‑150 (MQ=255)
                                                                                         aCTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttt                                                      <  1:387305/151‑1 (MQ=255)
                                                                                              aGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTc                                                 <  1:763345/151‑1 (MQ=255)
                                                                                              aGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTc                                                 <  2:627645/151‑1 (MQ=255)
                                                                                              aGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTc                                                 >  1:117660/1‑151 (MQ=255)
                                                                                               gCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCg                                                <  2:144824/151‑1 (MQ=255)
                                                                                                 gTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCt                                              <  2:226847/151‑1 (MQ=255)
                                                                                                 gTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCt                                              <  1:1146987/151‑1 (MQ=255)
                                                                                                      tGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACa                                          >  2:991090/1‑150 (MQ=255)
                                                                                                         aGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGaa                                      <  1:333473/151‑1 (MQ=255)
                                                                                                          gTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGaaa                                     <  1:251503/151‑1 (MQ=255)
                                                                                                            gAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGaaaa                                    <  2:1236735/150‑1 (MQ=255)
                                                                                                            gAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGaaaa                                    <  1:531576/150‑1 (MQ=255)
                                                                                                              aTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAatat                                 <  2:377085/151‑1 (MQ=255)
                                                                                                                cGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTc                               >  2:160128/1‑151 (MQ=255)
                                                                                                                cGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTc                               <  2:90823/151‑1 (MQ=255)
                                                                                                                 gTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCg                              >  2:479012/1‑151 (MQ=255)
                                                                                                                      aTGCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCat                          >  2:826794/1‑150 (MQ=255)
                                                                                                                        gCACAATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCatat                        <  2:330998/150‑1 (MQ=255)
                                                                                                                         cacaATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatg                       >  1:1151285/1‑150 (MQ=255)
                                                                                                                           caATAAAAAAATCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATatgaat                    <  2:365542/151‑1 (MQ=255)
                                                                                                                               aaaaaaaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGAtt                >  2:1047362/1‑151 (MQ=255)
                                                                                                                                   aaaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACg            <  1:1170206/151‑1 (MQ=255)
                                                                                                                                    aaTCCCGACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGc           <  1:58396/151‑1 (MQ=255)
                                                                                                                                          gACCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTa     <  1:341815/151‑1 (MQ=255)
                                                                                                                                           aCCCTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTAt    <  2:791745/151‑1 (MQ=255)
                                                                                                                                             ccTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATg   >  2:253894/1‑150 (MQ=255)
                                                                                                                                             ccTGAGGGGGTCTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGa  <  2:1114680/151‑1 (MQ=255)
                                                                                                                                                       ||                                                                                                                                           
CCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGG‑‑TCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACAAGAAAATATTCGCCATATGAATGATTAACGCTACTTATGA  >  NC_000913/2025096‑2025385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: