Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,271,827	0	A	T	47.3%	63.2 / 61.7	74	V8E (GTA→GAA)	chaA	calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (2/37);  new base T (35/0);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 8.49e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGG  >  NC_000913/1271677‑1271977                                                                                                                                                        |                                                                                                                                                         acaacaCTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTa                                                                                                                                                          <  2:1131623/151‑1 (MQ=255)     cacTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTcccc                                                                                                                                                      >  2:604953/1‑149 (MQ=255)       cTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCGcc                                                                                                                                                     >  2:587697/1‑150 (MQ=255)         aaaGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCCCctc                                                                                                                                                   >  2:313837/1‑150 (MQ=255)           aGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTg                                                                                                                                                <  2:901789/151‑1 (MQ=255)           aGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCGCCCCtt                                                                                                                                                 >  1:638028/1‑150 (MQ=255)           aGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTATCGCctcct                                                                                                                                                 >  2:959273/1‑148 (MQ=255)                cTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCa                                                                                                                                            <  2:901527/150‑1 (MQ=255)                 tACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTTCCGCCTCTTTAGCAtt                                                                                                                                          >  2:380180/1‑151 (MQ=255)                     tAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCGCCTCTTGAGCAttt                                                                                                                                         >  1:9116/1‑148 (MQ=255)                      aaaaTACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACa                                                                                                                                     <  2:682108/151‑1 (MQ=255)                      aaaaTACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCGCCTCCTTGGCAttt                                                                                                                                         >  1:451632/1‑147 (MQ=255)                       aaaTACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTTTCCCCTCtttcggattt                                                                                                                                         >  2:37405/1‑138 (MQ=255)                          tACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCCCCCCTTTAGCATTTTTCAtt                                                                                                                                   >  2:225465/1‑149 (MQ=255)                           aCCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTAt                                                                                                                                 <  1:650446/150‑1 (MQ=255)                            ccAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGCTTTTTCCGCCCCTTGTGCCtttttcttttt                                                                                                                                 >  1:640881/1‑141 (MQ=255)                              aaTAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTTCCGCCTCTTTAGCATTTGAAATTATTGtt                                                                                                                             >  2:378477/1‑151 (MQ=255)                               aTAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTa                                                                                                                            <  2:1090553/151‑1 (MQ=255)                                 aaGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGAAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTAt                                                                                                                           <  1:543890/150‑1 (MQ=255)                                    cgcAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTTCCCCCCCTTTTGCATTTTATAtttttggttttcc                                                                                                                        >  1:872275/1‑139 (MQ=255)                                      cAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCtt                                                                                                                     <  1:396504/150‑1 (MQ=255)                                      cAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCTCCCCTTTTGCATTTTTCATTTTTGTTTTT‑CCttt                                                                                                                    >  1:694643/1‑151 (MQ=255)                                      cAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCCCCCCTTTTGCATTTTTCATTATTGtttttccctt                                                                                                                     >  2:1143948/1‑143 (MQ=255)                                      cAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCCCCTCTTGAGCATTTGAAATTATGGTTTTC‑CCctt                                                                                                                    >  2:787690/1‑151 (MQ=255)                                       aagaagATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCttt                                                                                                                    <  2:221930/150‑1 (MQ=255)                                       aagaagATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTg                                                                                                                   <  2:1081819/151‑1 (MQ=255)                                          aagaTTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAg                                                                                                                <  2:403967/151‑1 (MQ=255)                                          aagaTTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCGCCTCTTTTGCATTTTACATTTTTGTTATT‑CCctt                                                                                                                    >  1:317943/1‑147 (MQ=255)                                              ttGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCCCCCCTTTAGAAATTTACATTTTTGtttttccctt                                                                                                                     >  2:566371/1‑135 (MQ=255)                                               tGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGa                                                                                                            <  2:1003642/150‑1 (MQ=255)                                                 aTGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCCCCCCTTTAGGATTTTACATTTTTGTTTTT‑CCCTTTCAGGTGaa                                                                                                           >  2:527604/1‑149 (MQ=255)                                                   ggCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAAttt                                                                                                        <  1:1089067/150‑1 (MQ=255)                                                    gCAATGACCACTGGTGGTGTCTGGCTGCTTCCCCCCAGGAACAGCACTACCGGCGCAAGAACCGGGAAAATAAGCGAAGTGTCATTGTGGC‑GGGTTTTTAGCTCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAAttt                                                                                                        <  1:997366/149‑1 (MQ=255)                                                           ccACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAAt                                                                                              <  1:390709/151‑1 (MQ=255)                                                             aCTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGt                                                                                            <  1:1062370/151‑1 (MQ=255)                                                             aCTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCCCCCCTTTTGCATTTTACATTATTGTTTTT‑CCTTTTCAGATGAATTTATTT‑aaaa                                                                                               >  2:1109418/1‑148 (MQ=255)                                                              cTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCGCCTCTTTAGCATTTTTTATTATTGTTTTT‑CCTTTGCAGATGAATTTATTG‑aaaa                                                                                               >  2:102078/1‑147 (MQ=255)                                                               tGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCGCCCCCTTAGCAATTTTCATTTTTGTTTTT‑CCTTTTCCGATGGAtttttttaaaa                                                                                                >  2:242660/1‑138 (MQ=255)                                                                ggTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCCCCCCTTTTGCATTTGTCATTTTGGTTTTT‑CCTTTTCAGATGAAtttttttaaaa                                                                                                >  1:589456/1‑137 (MQ=255)                                                                 gTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTaaaa                                                                                        <  2:1066579/151‑1 (MQ=255)                                                                  tAGTGTCTGGCTGCTTCCCCACAGGAACGGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTaaaa                                                                                        <  1:806497/150‑1 (MQ=255)                                                                     tgtCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGCGTTTTTTCGCCCCTTTTGGACTTTTCATTTTTGTTTTC‑CCTTTTCAGATGAATTTTTTG‑AAAATATaaaaaa                                                                                      >  1:894510/1‑149 (MQ=255)                                                                          ggCTGCTTCCCCACAGGAACAGCGCTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAAtaggta                                                                                <  1:223973/150‑1 (MQ=255)                                                                          ggCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAAtaggta                                                                                <  2:461831/150‑1 (MQ=255)                                                                          ggCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAAtaggta                                                                                <  1:587692/150‑1 (MQ=255)                                                                              gctTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTTCCCCCCCTTG‑GCATTTTTCATTTTTGTTTTT‑CCTTTTCAGATGAAATTTTTT‑AAAAATTAAAAAAAAGGGAGGaa                                                                            >  1:475684/1‑150 (MQ=255)                                                                                ttCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGCTTTTTTCCCCCCTTTTGACTTTTACATTTTTGTTTTCTCCTTTTAAG‑GGAATTTTTCC‑GAAAAGTAAAAAAAAAGTAGGaaaa                                                                          >  1:345527/1‑150 (MQ=255)                                                                                 tCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGaaaaa                                                                         <  2:643358/150‑1 (MQ=255)                                                                                 tCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGaaaa                                                                          <  1:70910/149‑1 (MQ=255)                                                                                     cacaGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCCCCCCTTTAGGATTTTTCATTATTGTTATT‑CCCTTTCAAGTGAAATTTTTG‑AAAAAGTAAAAAAAAAGGAGGaaaaaaaa                                                                      >  2:679193/1‑149 (MQ=255)                                                                                       caGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCCCCTCCTTAGCATTTTACATTTTGGGTTTT‑CCTTTTCCGATGAATTTTTTGTAAAATTAAAAAAAAAGGAAGaaaaaaaa                                                                       >  2:548596/1‑145 (MQ=255)                                                                                            aCAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAgtg                                                             <  1:180087/151‑1 (MQ=255)                                                                                            aCAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCGCCCCTTTTGCATTTGACATTTTTGTTTTT‑CCTTTTCAGATGAATTTTTTG‑AAAATGTAAAAAAAAGGTAGGGAAAAAAAAAGaaa                                                                >  1:760055/1‑148 (MQ=255)                                                                                             cAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAgtgt                                                            <  1:673035/151‑1 (MQ=255)                                                                                                   tACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTACGCCCCTTGAGGAATTTTAATTTTGGTTTTT‑CCTTTGCCGATGGATTTTTCG‑AAAAAGTAAAAAAAAAGGAGGaaaaaaaaaaaaaa                                                                >  1:399792/1‑141 (MQ=255)                                                                                                       aGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCg                                                  <  2:1054369/151‑1 (MQ=255)                                                                                                       aGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCCCCCCTTTAGGATTTTACATTTTTGTTTTT‑CCTTTTCTGATGAATTTTTTG‑AAAATGTAAAAAAAAAGGAGGAAAAAAAAAAAAAAAAGTCTgggt                                                      >  1:31248/1‑145 (MQ=255)                                                                                                              gAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTTCCCCCCTTTAGCATTTTACATTTTTGTTTTT‑CCCTTTCAGGTGAATTTTTTG‑AAAAAGTAAAAAAAAAGTAGGGAAAAAAAAAAAAAAAGGCTGGGTATTGGGAACa                                            >  2:411431/1‑150 (MQ=255)                                                                                                                 ccGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACAtttt                                        <  1:37405/151‑1 (MQ=255)                                                                                                                  cGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTTTCGCCCCTTTAGCATTTGACATTATTGTTATT‑CCTTTGCAGATGAATTTTTTT‑AAAAAGTAAAAAAAAAGTAGGAAAAAAAAAAAAAAGAGTCTGGATATTGGTAAAAattt                                        >  1:22625/1‑150 (MQ=255)                                                                                                                  cGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCGCCCCTTTAGCATTTTACATTATGGTTATC‑CCTTTTCAGATGAATTTATTG‑AAAATGTAAAAAATAGGGAGGAAAAAAAAAAAAAAGAGGCTGGGTATCGGGAAAAtttt                                        >  2:440847/1‑150 (MQ=255)                                                                                                                   gggAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTTCCGCCCCCTGTGCATTTGTAATTTTTGTTATT‑CCTTTTCCGGTGAATTTTTTG‑AAAATGTAAAAAATAAGTAGGGAAAATAAAAAAAAGTGGTTGGGTATTGGTAACAtttt                                        >  2:256205/1‑149 (MQ=255)                                                                                                                    ggAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTACC‑CCCCTTGAGCATTTGACATTATGGGTTTC‑CCTTTTCAGATGAATTTATTG‑AAAATATAAAAAAAAGGTAGGAAAAATAAAAAAAAAGGTCTGGATATCGGTAAAAattt                                        >  2:157291/1‑148 (MQ=255)                                                                                                                    ggAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACg                                     <  1:378474/151‑1 (MQ=255)                                                                                                                      aaaaTAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGaa                                   <  1:527599/151‑1 (MQ=255)                                                                                                                         aTAAGCGAAGTCTCCTTGTGGC‑GGGGTTTTTCCGCCTCTTTAGGATTTTACATTTTGGTTTTT‑CCTTTTCAGATGAATTTTTTG‑AAAAATTAAAAAAAAAGGAGGGAAAAAAAAAAAAAAAGTCTGGGTATCGGTAAAAATTTATGAAatt                                >  1:518777/1‑151 (MQ=255)                                                                                                                           aaGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAAttttt                              <  1:1174453/151‑1 (MQ=255)                                                                                                                              cGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTAc                            <  2:622473/150‑1 (MQ=255)                                                                                                                                      ccTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCt                      <  1:1100841/148‑1 (MQ=255)                                                                                                                                      ccTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTc                   <  2:1128517/151‑1 (MQ=255)                                                                                                                                         tgtgGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTggag                  <  2:839074/149‑4 (MQ=255)                                                                                                                                          gtgGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGAt                <  1:1001637/150‑1 (MQ=255)                                                                                                                                          gtgGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTCTACCCCGCTGTCGAt                <  1:242658/150‑1 (MQ=255)                                                                                                                                                    ttttACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTc       <  2:605252/150‑1 (MQ=255)                                                                                                                                                     tttACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCa      <  1:987055/150‑1 (MQ=255)                                                                                                                                                        aCCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTgg  <  1:853646/151‑1 (MQ=255)                                                                                                                                                        aCCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTg   <  1:242679/150‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                         ACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGC‑GGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATC‑CCTTTGCAGATGAATTTATCG‑AAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGG  >  NC_000913/1271677‑1271977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑