Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,177,748	0	C	A	44.3%	137.7 / 58.0	97	E55* (GAG→TAG)	nudF	ADP‑ribose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (43/11);  new base A (0/43);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 1.96e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTT  >  NC_000913/3177598‑3177895                                                                                                                                                        |                                                                                                                                                    cacTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTc                                                                                                                                                     >  2:952512/1‑151 (MQ=255)      tCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCaaaa                                                                                                                                                 <  1:1138669/150‑1 (MQ=255)       cACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAAt                                                                                                                                               >  1:895757/1‑151 (MQ=255)        aCCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAAtt                                                                                                                                              <  2:305565/151‑1 (MQ=255)            tctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTccc                                                                                                                                          >  1:744028/1‑151 (MQ=255)             ctTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTcc                                                                                                                                           <  2:810219/149‑1 (MQ=255)                 aaTCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCcgccgc                                                                                                                                     >  2:280866/1‑151 (MQ=255)                     atcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCt                                                                                                                                 >  2:990435/1‑151 (MQ=255)                     atcCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCt                                                                                                                                 >  1:245836/1‑151 (MQ=255)                        ccGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCa                                                                                                                               >  2:534216/1‑150 (MQ=255)                                cATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAtt                                                                                                                      >  2:1148844/1‑151 (MQ=255)                                cATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAtt                                                                                                                      >  2:385511/1‑151 (MQ=255)                                 aTCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCAttt                                                                                                                     >  1:302431/1‑151 (MQ=255)                                     ccAGTAGCCAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGccc                                                                                                                 <  2:376089/151‑1 (MQ=255)                                     ccAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCGTCTCAATCAGCACAACTTCATCGCGCACTGGGTCAAAGGGTAACAAGGCTGCGGCGTG‑CCCCCGCTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGccc                                                                                                                 <  1:90358/151‑1 (MQ=255)                                     ccAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGCCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTg                                                                                                                    <  2:884107/148‑1 (MQ=255)                                      cAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                                >  2:53225/1‑151 (MQ=255)                                      cAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCg                                                                                                                >  2:314207/1‑151 (MQ=255)                                        gTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGtt                                                                                                              >  2:929238/1‑151 (MQ=255)                                          aGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                             >  1:1135830/1‑150 (MQ=255)                                            ccAGGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAACCAGCACACCTTCATCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                             <  2:511755/148‑1 (MQ=255)                                             cAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  1:941739/1‑150 (MQ=255)                                             cAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  1:523116/1‑150 (MQ=255)                                              aggggtTTTCGCGGGTGTCGTACGCGGCAACCCGAATCTGCACAATCAGCACAACTTCACCCCGCCCTGGGTCAAGGGGTAGCAAGACTGCGGCGTG‑CCCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATa                                                                                                         <  1:725716/148‑1 (MQ=255)                                              aaGGGTTTTCGCTGGTTTCGTCCGCGGCAACCCAAATCTGCTCAATCAGCACAACTTCACCACGCGCTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGaa                                                                                                           <  1:591151/148‑1 (MQ=255)                                              aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  2:279504/1‑149 (MQ=255)                                              aaGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAAt                                                                                                          >  2:147486/1‑149 (MQ=255)                                              aaGGGGTTTCGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTg                                                                                                             <  1:60162/146‑1 (MQ=255)                                                  ggTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACg                                                                                                     >  2:993765/1‑150 (MQ=255)                                                    tttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                                                                                                   <  2:549097/150‑1 (MQ=255)                                                    tttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                  >  2:990322/1‑151 (MQ=255)                                                    tttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATg                                                                                                  >  2:1104505/1‑151 (MQ=255)                                                    tttCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCACCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGAt                                                                                                   <  2:895765/150‑1 (MQ=255)                                                       cGCTGGTGTCGTACGCGGCAACCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGAc                                                                                                <  2:708578/150‑1 (MQ=255)                                                        gttGGTGTCGTACGCGGCAATCCGAATCTGCTCAACCACCACAACTCCACCACGCACGGGGCAAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACg                                                                                               <  1:587291/148‑1 (MQ=255)                                                            gtgtCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATc                                                                                          >  1:1152277/1‑151 (MQ=255)                                                              gtCGTACGCGGCAATCCGAATCTGCTAAATCAGCACACCTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCta                                                                                        <  1:239612/151‑1 (MQ=255)                                                                cGTACGCGGCAATCCGAATCTGCTCAATCAGCACACCTTCATCACGCCCTGGGTCAAAGGGTAGCAGGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCta                                                                                        <  2:497911/149‑1 (MQ=255)                                                                   aCGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                     >  2:997067/1‑149 (MQ=255)                                                                    cgcgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCCCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAg                                                                                   <  2:425437/150‑1 (MQ=255)                                                                      cgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCCCGGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                                 <  2:608840/150‑1 (MQ=255)                                                                      cgGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATaa                                                                                     >  2:155977/1‑146 (MQ=255)                                                                       ggCAACCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACGGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                                 <  1:553322/149‑1 (MQ=255)                                                                       gccactCCGATTCTACTAAAGAAGCGCAACTTCACCCCTCACTGGTCCAACGGGAAGCAA‑CCGTCCGCGAGCACGGCGCCAAAAAATTGCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGAt                                                                                 <  2:586240/144‑1 (MQ=255)                                                                         cAATCCGAATCTGCTCAACCAGCACAACTTCATCCCGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATc                                                                                <  2:659356/148‑1 (MQ=255)                                                                          aaccgaaaTCTGCCAAATCAGCACAGCCGCACCACGCCTGGGGACAAAGGAAACCAAGACTGGGGCGGT‑ACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              <  1:1114551/143‑1 (MQ=255)                                                                          aaTCCGAATCTGCTCAATCAGCACAATTTCATCCCGCACGGGGTCAAGGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTa                                                                              <  2:644815/149‑1 (MQ=255)                                                                             ccaaaTCTCCTCAACCAGCACAACTTCATCCCGCCTTGGGTAAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATg                                                                           <  1:266817/146‑1 (MQ=255)                                                                             ccGAATCTGCTCAATCAGCACAACTTCACCCCGCTCTGGGTCAAAGGGTAGCAAGACTGCGGGTTG‑ACCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                         <  2:306407/151‑1 (MQ=255)                                                                             ccGAATCTGCTCAATCACCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                         <  2:1034443/151‑1 (MQ=255)                                                                              cGAATCTGCTCAATCAGCCCAACTTCACCACGCACTGGGTAAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa                                                                        <  2:218632/151‑1 (MQ=255)                                                                              cGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaa                                                                         >  1:83589/1‑150 (MQ=255)                                                                              cGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa                                                                        <  2:83736/151‑1 (MQ=255)                                                                                aaTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaa                                                                        <  2:218181/149‑1 (MQ=255)                                                                                aaTCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaa                                                                       >  2:234776/1‑150 (MQ=255)                                                                                  tCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaaa                                                                     >  1:1164726/1‑150 (MQ=255)                                                                                  tCTGCCAAACCAGCACACCTGCACCCCGCATGGGTTCAAAGGGAAGAAAGGCGGGGGGGTA‑CCCGGGCTAAAAAATTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGaaaaa                                                                      <  2:301389/149‑1 (MQ=255)                                                                                         aaccAGCACACCTCCATCACGCACGGGGTAAAAGGGAAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                                <  2:1124843/145‑1 (MQ=255)                                                                                         aaTCAGCACAACTTCATCCCGCACGGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGcc                                                                 <  1:851942/147‑1 (MQ=255)                                                                                         aaTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                                <  2:510438/148‑1 (MQ=255)                                                                                         aaTCAGCACAACTTCATCACGCACGGGGTAAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcg                                                                <  1:824870/148‑1 (MQ=255)                                                                                         aaTCAGCACAACTTCACCACGCACTGGGTCAAGGGGTAGCAAGCCTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATG‑AAAAAAGCCGCGAt                                                            <  2:71965/151‑1 (MQ=255)                                                                                         aaTCAGCAAAACTTCACCACGCACTGGGAAAAGGGTTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCcgcg                                                              <  1:569159/150‑1 (MQ=255)                                                                                          aTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATATACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGa                                                             >  1:643816/1‑150 (MQ=255)                                                                                          aTCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGAt                                                            >  1:253614/1‑151 (MQ=255)                                                                                             aGCACACCTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGGG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATa                                                           <  1:964961/149‑1 (MQ=255)                                                                                               cacaACTACACCACCCACTGGGTCAAAGGGTAGCAAGACTTCGGCGTA‑CCCGCGCTCAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATa                                                           <  1:331476/147‑1 (MQ=255)                                                                                                acaACTTCATCACGCACTGGGTCAAGGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg                                                      <  1:1370/151‑1 (MQ=255)                                                                                                acaACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAgtg                                                      >  2:627324/1‑151 (MQ=255)                                                                                                  aaCTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGtt                                                    >  1:805381/1‑151 (MQ=255)                                                                                                    ctccaccaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGttt                                                   <  1:602492/144‑1 (MQ=255)                                                                                                      tcatcaCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTtctc                                                >  2:38323/1‑151 (MQ=255)                                                                                                          caCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑CCCGCCCAAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCg                                               <  2:467810/148‑1 (MQ=255)                                                                                                          caCGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                             <  2:1173127/150‑1 (MQ=255)                                                                                                          caCGCACTGGGTCAAAGGGTAGAAAGACTGCGGCGTG‑ACCGCCCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTg                                             <  2:1122151/150‑1 (MQ=255)                                                                                                             gCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         >  1:633530/1‑151 (MQ=255)                                                                                                              cACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         >  1:53076/1‑150 (MQ=255)                                                                                                              cACTGGGTCAAAGGGTAGCAAGACGGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         <  1:378997/150‑1 (MQ=255)                                                                                                              cACTGGGTAAAAGGGAAGCAAGACTGCGGCGTG‑ACCGCGATAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaat                                         <  1:953464/150‑1 (MQ=255)                                                                                                                  gggTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAAttt                                    >  2:1090169/1‑151 (MQ=255)                                                                                                                  gggTCAAAGGGTAGCAAGACGGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCaataat                                      <  2:78997/149‑1 (MQ=255)                                                                                                                    gTCAAAGGGTAGCAAGCCTGCGGCGTG‑CCCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                  <  2:1113932/151‑1 (MQ=255)                                                                                                                     tCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCt                                  >  1:325669/1‑150 (MQ=255)                                                                                                                       aaaGGGTAGCAAGCTTGGGGCGTG‑ACCGCGCTAAAAATTTCCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTa                                 <  1:553375/149‑1 (MQ=255)                                                                                                                       aaaGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTAc                                >  1:611966/1‑150 (MQ=255)                                                                                                                        aaGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACAt                              >  1:204846/1‑151 (MQ=255)                                                                                                                              aGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGtttt                        >  2:3995/1‑151 (MQ=255)                                                                                                                                 aaGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGc                     >  1:935037/1‑151 (MQ=255)                                                                                                                                   gACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGcc                    >  2:1227317/1‑150 (MQ=255)                                                                                                                                   gACTGCGGCGTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCa                   <  2:119614/151‑1 (MQ=255)                                                                                                                                         ggcgTG‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGt              <  1:835044/150‑1 (MQ=255)                                                                                                                                           cgTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAAc           >  1:913178/1‑151 (MQ=255)                                                                                                                                             tG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACgg         >  1:1027196/1‑151 (MQ=255)                                                                                                                                              g‑ACCGCGCTAAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACg          <  1:763917/149‑1 (MQ=255)                                                                                                                                                 ccGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACggg        <  2:409098/149‑1 (MQ=255)                                                                                                                                                 ccGCACTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACgg         <  1:273298/148‑1 (MQ=255)                                                                                                                                                    cgcTAAAAATTTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAgg    <  1:1161381/150‑1 (MQ=255)                                                                                                                                                     gcTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGtt  >  1:941875/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                    CACTTTCACCCTCTTCAATCATCCCGGCAACCATCTCCAGTAGCCAAGGGGTTTCGCTGGTGTCGTACGCGGCAATCCGAATCTGCTCAATCAGCACAACTTCATCACGCACTGGGTCAAAGGGTAGCAAGACTGCGGCGTG‑ACCGCGCTCAAAAATTTCCCGCCGCACCTCATGACTCATTTGCCCGTTGAATAGACGATGACGAAATCTATAAAGATCTAATGAAAAAAAGCCGCGATAAAGTGTTTCTCGTGCAATAATTTCTACATCGTTTTTGCCAAATGTAACGGGCAGGTT  >  NC_000913/3177598‑3177895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑