Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC46.6% 149.1 / 27.3 88V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (44/3);  new base C (0/41);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.24e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427
                                                                                                                                                       |                                                                                                                                                      
cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACACCGAAAGTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                       <  1:990353/151‑1 (MQ=255)
       aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                                >  1:977172/1‑151 (MQ=255)
         agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                              >  1:1176117/1‑151 (MQ=255)
           agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCatat                                                                                                                                            >  2:999494/1‑151 (MQ=255)
               acacATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCAc                                                                                                                                        >  2:734707/1‑151 (MQ=255)
                cacaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACt                                                                                                                                       >  1:353248/1‑151 (MQ=255)
                 acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                                      >  1:298319/1‑151 (MQ=255)
                   aaaaggCGGTGCCCCGGGCTCAAAATACAGCAAATTTGTTGCCAGCCGGTCCCACCCAGTACCGCCCCCAACCCAGGACGCGATGGTCGCCGGGAACAAAAAAATACGGGAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACttt                                                                                                                                     <  2:372170/145‑1 (MQ=255)
                   aTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                    >  1:29568/1‑151 (MQ=255)
                    tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                    >  1:573180/1‑150 (MQ=255)
                     aaggggtttccccGGGCTAAAAATACAGCAAATTTGTTGTCACCCATTCCCACCCAGTCCCGCCCCCGAACCAGGACGGGTTGGTCGCGGGAAAAAAAATAATACTGGAAGCAAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAg                                                                                                                                  <  2:141022/141‑1 (MQ=255)
                     aaggctgTTGCCGGGGCTAAAAATACAGCAAATTTTTTGTCCCCCGTTCCCACCCAGTCCCCCCCCCGACCCATGGCGGGATGGCCGCCGGAAACAAAAAAATACTGAAAGGAAAGATTGCCGGTATTCCCCCCGCCATATTCACTTTCAg                                                                                                                                  <  1:99270/145‑1 (MQ=255)
                       aGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCg                                                                                                                                >  2:560165/1‑151 (MQ=255)
                         cGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                              >  2:221529/1‑151 (MQ=255)
                              ccccAGCCTCAAAAAACGGCAAATTTGTTGCCCGCCGTTCCCAACCGGTCCCCCCCCCGAACCATGCCGGGAGGGTCGCGGGAAAAAAAATAATACTGGAGGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         <  1:1049217/150‑1 (MQ=255)
                               cccGGGCTGAAAATAAAGAAAATTTTTTGCCAGCCATTCCCACCCAGTCCCCCCCCCGAACCATGCCGGGATGGCCGCGGGGAAAAAAATAATCCTGAAGGCGAAGATGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                          <  2:849567/149‑1 (MQ=255)
                               cccAGGCTGAAAATACAGGAAAATTGTTGTCAGCCAGTTCCAACCAGCCCCCCCCCCAAACCATGACGGGATGGTCGCGGGGAAAAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAt                                                                                                                          <  1:471121/149‑1 (MQ=255)
                                 cAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                      >  1:817881/1‑151 (MQ=255)
                                   ggCTGAAAATACAGCGAATTTTTTGCCAGCCAGTCCCACCCAGCCCCCCCCCCAAACCAGGCCGGGATGGTCGCGGGAAAAAAAATAATACTGGAAGGGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGc                                                                                                                    <  1:538316/151‑1 (MQ=255)
                                     cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  >  2:485048/1‑151 (MQ=255)
                                     cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  >  2:1130807/1‑151 (MQ=255)
                                     cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  >  2:102449/1‑151 (MQ=255)
                                      tGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                                 >  1:1148422/1‑151 (MQ=255)
                                       gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                >  2:139929/1‑151 (MQ=255)
                                         aaaaacaaaaaaaTTGTTTCCACCAATTCCCACCCAGTCCCCCCCCCAAACAATGACGCGATGGCCGCGGGAAAAAGAAAAATACGGGAGGCGAAATTGGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  <  1:427481/137‑1 (MQ=255)
                                         aaaTACAGCAAATTTTTTGTCAGCCAGTCCCAACCAGTCCCGCCCCCGAACCATGACGGGATGGTCGCCGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                               <  2:969050/150‑1 (MQ=255)
                                         aaaTACAGCAAATTTTTTGCCACCCATTCCCACCCAGTCCCCCCCCCGAACCATGACGCGATGGTCGCCGGAAAAAAAATAATACTGGAAGCGAAGTTTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                                <  2:791014/149‑1 (MQ=255)
                                          aaaacagaaaaaTTGTTGTCAGCCAGTCCAAACGAGTCCCCCCCCCAACCCATGGCGGGGGGGTCGCGGGAAACAAAAAAATAGTGGAAGCAAGGCGTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                             <  1:1216349/142‑1 (MQ=255)
                                                  gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:603767/1‑148 (MQ=255)
                                                   aatttgttgCCAGCCGGTTCCACCCAGTCCCCCCCCCCAACCATGACGAGCTGGCCGCCGGAAACAGAATAATACTGAAGGCGAAGATTGCGGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                    <  2:433053/148‑1 (MQ=255)
                                                   aatttgttgCCACCAATTCCCGACCAGCCCCGCCCCCAAACCATGGCGAGAGGGTCGCCGGAAACAAAAAAAGACGGAAGGAAAGGGTGGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATa                                                                                                       <  1:951987/145‑1 (MQ=255)
                                                   aaaTTTTTGTCAGCCAGTCCCACCCGGTCCCCCCCCCAACCCATGACGCGATGGTCGCGGGAAACAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                          <  2:565150/145‑1 (MQ=255)
                                                   aaaTTGTTGCCCGCCATTCCCACCCTGTCCCCCCCCCGAACCAGGCCGCGATGGTCGCGGGGAAAAAAATAATAGGGGAAGAGAATTTGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  1:1235875/147‑1 (MQ=255)
                                                      ttgttgTCACCCAGTCCCACCCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGAAAAAAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  <  1:987660/150‑1 (MQ=255)
                                                             cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                          >  2:337551/1‑151 (MQ=255)
                                                                ccattcccACCCAGTCCCCCCCCCGACCCATGACGCGATGGTCGCGGGAAACAAAATAATACTGGAGGCAAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         <  1:505200/143‑1 (MQ=255)
                                                                   gTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     >  1:603226/1‑150 (MQ=255)
                                                                   gTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     >  1:28458/1‑150 (MQ=255)
                                                                      ccACCCAGCCCCCCCCCCAAACAATGACGCGATGGTCGCCGGAAACAAAAAAATACTGGAAGCAAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  1:742516/150‑1 (MQ=255)
                                                                      ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                                 >  1:1180703/1‑151 (MQ=255)
                                                                        aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                               >  1:380016/1‑151 (MQ=255)
                                                                          ccAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                             >  1:52754/1‑151 (MQ=255)
                                                                          ccAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                             >  2:878396/1‑151 (MQ=255)
                                                                            aGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgcg                                                                           >  2:858853/1‑151 (MQ=255)
                                                                             gcccccccccccACCCAGGGCGGGATGGCCGCGGGAAAAAAAAAAATACGGGAAGGGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                            <  1:258684/143‑1 (MQ=255)
                                                                                 cccccccGACCCAGGACGCGTTGGTCGCGGGAAACAAAATAATCCTGAAGGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      <  2:533183/149‑1 (MQ=255)
                                                                                 cGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      >  1:879809/1‑151 (MQ=255)
                                                                                 cGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      >  1:677027/1‑151 (MQ=255)
                                                                                 cGCCCCCGAACCAGGACGCGTTGGTCGCCGGGAACAAAATAATACTGGAAGCGAAGATTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      <  2:290616/151‑1 (MQ=255)
                                                                                  gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     >  2:23580/1‑151 (MQ=255)
                                                                                   ccccgGAACCAGGAGGCGAGGGTCGCGGGAAACAAAAAAATACTGAAGGCGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTa                                                                    <  2:59208/151‑1 (MQ=255)
                                                                                      ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                  >  2:543473/1‑150 (MQ=255)
                                                                                        gAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                                >  2:1060412/1‑150 (MQ=255)
                                                                                         aaCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                               >  1:600736/1‑150 (MQ=255)
                                                                                           ccATGACGCGAGGGTCGCCGGAAAAAAAATAATCCTGGAGGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             <  2:38658/150‑1 (MQ=255)
                                                                                                  gggATGGCCGCCGGGAAAAAAATAATCCTGGAAGCGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                        <  1:592185/146‑1 (MQ=255)
                                                                                                   gtttggcccccGGAAACAGAATAAATAGGGAGGAGAAATTTGCGGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                      <  2:610553/140‑1 (MQ=255)
                                                                                                       ggTCGCGGGGAACAGAATAATACTGGAAGGGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                            <  2:503353/139‑1 (MQ=255)
                                                                                                             cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           >  2:771384/1‑150 (MQ=255)
                                                                                                             cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           >  2:1113790/1‑150 (MQ=255)
                                                                                                              ggaaaaaaaaaaataCTGGAAGCGAAGTTGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  2:28458/140‑1 (MQ=255)
                                                                                                              ggaaaaaaaaaaataCGGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  2:288775/140‑1 (MQ=255)
                                                                                                              ggaaaCAAAATAATGCGGGAAGCAAAGTTTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  2:931207/148‑1 (MQ=255)
                                                                                                                gAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                        >  2:803583/1‑150 (MQ=255)
                                                                                                                 aaaaaaataatACTGGAAGCGAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                       <  1:860996/145‑1 (MQ=255)
                                                                                                                   cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    >  1:640525/1‑151 (MQ=255)
                                                                                                                    aaaatattcggggAAGCAAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  1:480393/140‑1 (MQ=255)
                                                                                                                    aaaataatACTGGAGGCAAAGTTTCCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  1:1162154/149‑1 (MQ=255)
                                                                                                                    aaaaaaataCGGGAAGCGAAGATGGCCGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  1:696418/146‑1 (MQ=255)
                                                                                                                    aGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    >  1:631227/1‑150 (MQ=255)
                                                                                                                      aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  2:823559/1‑150 (MQ=255)
                                                                                                                      aataatACGGGAAGCAAAGATTGCGGTAATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 <  2:702946/151‑1 (MQ=255)
                                                                                                                       ataatgCTGCAAGATAAGATGGCGGGGATACCCTCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  <  1:26449/149‑1 (MQ=255)
                                                                                                                       ataataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                                 >  2:728958/1‑150 (MQ=255)
                                                                                                                        taataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               >  2:64290/1‑151 (MQ=255)
                                                                                                                        taataCTGGAAGAAAAGTGTGCTGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaa                                <  1:677931/150‑1 (MQ=255)
                                                                                                                         aatccgggAAGCAAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                              <  2:357978/145‑1 (MQ=255)
                                                                                                                         aataCTGGAAGAGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               <  2:397327/150‑1 (MQ=255)
                                                                                                                             cggaaaGCGAAGCTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAg                           <  1:499886/146‑1 (MQ=255)
                                                                                                                                 aaGCAAAGTTGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                          <  1:444307/147‑1 (MQ=255)
                                                                                                                                          aTTGCTGGCATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa              <  2:603231/151‑1 (MQ=255)
                                                                                                                                          aTTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa               >  1:827728/1‑150 (MQ=255)
                                                                                                                                              cggggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata           <  2:1173778/148‑1 (MQ=255)
                                                                                                                                              ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac          >  2:1028123/1‑151 (MQ=255)
                                                                                                                                               cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  2:455946/1‑151 (MQ=255)
                                                                                                                                                gggTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa               <  2:115245/144‑1 (MQ=255)
                                                                                                                                                gggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  2:942006/1‑150 (MQ=255)
                                                                                                                                                     ttACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaatac    >  2:341418/1‑151 (MQ=255)
                                                                                                                                                       aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  2:417470/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                      
CAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443128‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: