Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 99,891 C→T A83V (GCC→GTC)  murG → N‑acetylglucosaminyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091399,8910CT98.9% 320.5 / ‑4.3 87A83V (GCC→GTC) murGN‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (40/46);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCT  >  NC_000913/99744‑100037
                                                                                                                                                   |                                                                                                                                                  
ggCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTg                                                                                                                                                 <  2:141259/151‑1 (MQ=255)
  cAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGc                                                                                                                                               <  2:134460/151‑1 (MQ=255)
  cAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGc                                                                                                                                               <  2:399995/151‑1 (MQ=255)
   aaGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCg                                                                                                                                              <  1:404130/151‑1 (MQ=255)
   aaGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCg                                                                                                                                              >  2:327601/1‑151 (MQ=255)
    aGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGt                                                                                                                                             <  1:322149/151‑1 (MQ=255)
         gctggctgGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGgc                                                                                                                                        <  2:739620/151‑1 (MQ=255)
           tggctggGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGgcgc                                                                                                                                      >  2:709990/1‑151 (MQ=255)
            ggctggGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGgcgc                                                                                                                                      <  2:264131/150‑1 (MQ=255)
                ggggACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCggg                                                                                                                                   <  2:1023716/149‑1 (MQ=255)
                  gcaCTGCCGACCGTATGGAACCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGAt                                                                                                                               <  2:1017034/149‑1 (MQ=255)
                      tGCCGTCCGTATGGAAGCGGACTTAGCGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGTTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTAt                                                                                                                            >  1:956963/1‑150 (MQ=255)
                        ccGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         <  1:236216/151‑1 (MQ=255)
                        ccGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         <  2:1087840/151‑1 (MQ=255)
                         cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         >  2:390523/1‑150 (MQ=255)
                         cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         >  1:132035/1‑150 (MQ=255)
                         cGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGaa                                                                                                                         >  2:799107/1‑150 (MQ=255)
                          gACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAg                                                                                                                       <  1:158733/151‑1 (MQ=255)
                              gTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTa                                                                                                                   <  1:642333/151‑1 (MQ=255)
                                aTGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACa                                                                                                                 <  2:1131011/151‑1 (MQ=255)
                                  ggAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACaa                                                                                                                <  1:456635/150‑1 (MQ=255)
                                        ggACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGAc                                                                                                         <  2:26274/151‑1 (MQ=255)
                                        ggACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGAc                                                                                                         <  2:513375/151‑1 (MQ=255)
                                           cTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACgt                                                                                                       <  2:542271/150‑1 (MQ=255)
                                             tAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACgtg                                                                                                      <  2:816693/149‑1 (MQ=255)
                                               gTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGc                                                                                                  <  1:5296/151‑1 (MQ=255)
                                                  ccAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCg                                                                                               >  1:902292/1‑151 (MQ=255)
                                                    aaaaCATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGt                                                                                             >  2:1049582/1‑151 (MQ=255)
                                                     aaaCATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGt                                                                                             >  2:1188946/1‑150 (MQ=255)
                                                      aaCATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTAt                                                                                           <  1:596650/151‑1 (MQ=255)
                                                        cATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATgg                                                                                         >  2:985333/1‑151 (MQ=255)
                                                               tCGAAATTGATTTCATTCGTATCTCTGGTCTGCGGGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCt                                                                                   >  1:491677/1‑150 (MQ=255)
                                                                cGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTAc                                                                                 <  2:1170880/151‑1 (MQ=255)
                                                                 gAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACg                                                                                >  1:1066534/1‑151 (MQ=255)
                                                                 gAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACg                                                                                >  2:539776/1‑151 (MQ=255)
                                                                  aaaTTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACg                                                                                >  1:859263/1‑150 (MQ=255)
                                                                   aaTTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACgtg                                                                              <  1:606017/151‑1 (MQ=255)
                                                                          ttCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTc                                                                       >  1:611010/1‑151 (MQ=255)
                                                                            cATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCa                                                                     <  1:1089093/151‑1 (MQ=255)
                                                                            cATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCa                                                                     <  2:25678/151‑1 (MQ=255)
                                                                             aTTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAg                                                                    <  2:51812/151‑1 (MQ=255)
                                                                                cGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggtg                                                                 >  1:948772/1‑151 (MQ=255)
                                                                                cGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggtg                                                                 >  1:703614/1‑151 (MQ=255)
                                                                                cGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggt                                                                  <  2:1119998/150‑1 (MQ=255)
                                                                                  tATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggtggt                                                               >  2:496933/1‑151 (MQ=255)
                                                                                   aTCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAggtggt                                                               <  1:306209/150‑1 (MQ=255)
                                                                                       ctGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCt                                                             <  2:445974/148‑1 (MQ=255)
                                                                                       ctGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGc                                                          >  1:224123/1‑151 (MQ=255)
                                                                                        tGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGcc                                                         >  1:148580/1‑151 (MQ=255)
                                                                                         ggTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTgg                                                           <  1:880498/148‑1 (MQ=255)
                                                                                         ggTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGcc                                                         <  2:54684/150‑1 (MQ=255)
                                                                                         ggTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCcg                                                        >  1:198346/1‑151 (MQ=255)
                                                                                             tGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTg                                                    >  1:599321/1‑151 (MQ=255)
                                                                                               cGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGt                                                  >  2:1032590/1‑151 (MQ=255)
                                                                                                gTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTc                                                 <  2:953284/151‑1 (MQ=255)
                                                                                                gTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTc                                                 >  2:1002331/1‑151 (MQ=255)
                                                                                                    aaaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGtt                                              >  1:966193/1‑150 (MQ=255)
                                                                                                    aaaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGtt                                              >  2:374331/1‑150 (MQ=255)
                                                                                                     aaaGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAg                                            <  1:441516/151‑1 (MQ=255)
                                                                                                         gTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCAt                                        >  1:95448/1‑151 (MQ=255)
                                                                                                           ataAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTc                                      <  2:1223795/151‑1 (MQ=255)
                                                                                                            taAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTcc                                     >  1:12567/1‑151 (MQ=255)
                                                                                                             aaaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCg                                    >  2:1235780/1‑151 (MQ=255)
                                                                                                              aaaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCTCGTGGTCGTTAGGCATTCCgg                                   <  1:926792/151‑1 (MQ=255)
                                                                                                               aaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGt                                  <  2:270577/151‑1 (MQ=255)
                                                                                                               aaGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGt                                  >  2:399254/1‑151 (MQ=255)
                                                                                                                  cACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGt                               >  1:529641/1‑151 (MQ=255)
                                                                                                                  cACTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGt                               >  2:399920/1‑151 (MQ=255)
                                                                                                                   aCTGATAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGt                               >  1:286634/1‑150 (MQ=255)
                                                                                                                       aTAGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACtt                           >  1:289816/1‑150 (MQ=255)
                                                                                                                         aGCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCa                         <  2:491681/150‑1 (MQ=255)
                                                                                                                          gCTGCCCCGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATg                       <  1:993929/151‑1 (MQ=255)
                                                                                                                                ccGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACaaa                 <  2:1199051/151‑1 (MQ=255)
                                                                                                                                 cGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACaaaa                <  2:467592/151‑1 (MQ=255)
                                                                                                                                 cGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACaaa                 >  1:993480/1‑150 (MQ=255)
                                                                                                                                 cGCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACaaa                 >  2:586568/1‑150 (MQ=255)
                                                                                                                                  gCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAAc               <  1:1200329/151‑1 (MQ=255)
                                                                                                                                  gCTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAAc               <  1:859084/151‑1 (MQ=255)
                                                                                                                                   cTGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACg              >  2:1123758/1‑151 (MQ=255)
                                                                                                                                    tGCGTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACgg             >  1:981843/1‑151 (MQ=255)
                                                                                                                                       gTATCTTCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTAt          >  1:201817/1‑151 (MQ=255)
                                                                                                                                            ttCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCgg     >  2:887824/1‑151 (MQ=255)
                                                                                                                                             tCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGc       <  1:540799/148‑1 (MQ=255)
                                                                                                                                             tCAACGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCgg     <  1:854525/150‑1 (MQ=255)
                                                                                                                                               aaCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCt  >  1:1219140/1‑151 (MQ=255)
                                                                                                                                               aaCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCt  <  2:820468/151‑1 (MQ=255)
                                                                                                                                               aaCGTCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCt  <  2:971355/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCAAGTTCGCTGGCTGGGGACTGCCGACCGTATGGAAGCGGACTTAGTGCCAAAACATGGCATCGAAATTGATTTCATTCGTATCTCTGGTCTGCGTGGAAAAGGTATAAAAGCACTGATAGCTGCCCCGCTGCGTATCTTCAACGCCTGGCGTCAGGCGCGGGCGATTATGAAAGCGTACAAACCTGACGTGGTGCTCGGTATGGGAGGCTACGTGTCAGGTCCAGGTGGTCTGGCCGCGTGGTCGTTAGGCATTCCGGTTGTACTTCATGAACAAAACGGTATTGCGGGCT  >  NC_000913/99744‑100037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑