Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,761	G→T	A237E (GCG→GAG)	sapB ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,761	0	G	T	100.0%	219.4 / NA	56	A237E (GCG→GAG)	sapB	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/24);  total (32/24)

CGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGT  >  NC_000913/1354615‑1354906                                                                                                                                                   |                                                                                                                                                  cggAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGtt                                                                                                                                               <  2:920972/151‑1 (MQ=255) cggAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGtt                                                                                                                                               >  1:634745/1‑151 (MQ=255)  ggAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCCGCGCCCTAAACCAGGCCAGCTAAAAACCTTTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGCAAGCCCAGGCGCGGAATAACCGGCGGGAACTCGtt                                                                                                                                               >  1:102472/1‑150 (MQ=255)      aTGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCa                                                                                                                                          <  2:185936/151‑1 (MQ=255)       tGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGc                                                                                                                                           >  2:195363/1‑149 (MQ=255)              gTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTgg                                                                                                                                  <  1:790666/151‑1 (MQ=255)               tAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGc                                                                                                                                 >  1:377145/1‑151 (MQ=255)                aaTCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCg                                                                                                                                <  2:367855/151‑1 (MQ=255)                 aTCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCg                                                                                                                                >  2:793954/1‑150 (MQ=255)                         ggCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCaaa                                                                                                                         <  2:355700/149‑1 (MQ=255)                           cGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAAt                                                                                                                       >  2:552050/1‑149 (MQ=255)                                 gCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAgc                                                                                                               <  1:928952/151‑1 (MQ=255)                                     taattaaCCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGcgcg                                                                                                            <  2:302513/150‑1 (MQ=255)                                       attaaCCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGAc                                                                                                          >  1:462486/1‑150 (MQ=255)                                         taaCCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACaa                                                                                                        >  1:211291/1‑150 (MQ=255)                                            ccAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACcg                                                                                                    >  1:336353/1‑151 (MQ=255)                                                cgcCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCggg                                                                                                >  2:1223600/1‑151 (MQ=255)                                                 gcCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGt                                                                                               >  1:210045/1‑151 (MQ=255)                                                 gcCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGt                                                                                               <  2:512269/151‑1 (MQ=255)                                                    cTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgc                                                                                            >  2:155300/1‑151 (MQ=255)                                                      aaaCCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccg                                                                                          <  1:212294/151‑1 (MQ=255)                                                       aaCCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgc                                                                                         >  2:902351/1‑151 (MQ=255)                                                        aCCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTcgccgcc                                                                                        >  2:178432/1‑151 (MQ=255)                                                             gCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCttt                                                                                   >  1:1032708/1‑151 (MQ=255)                                                               cAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTa                                                                                 <  2:1042367/151‑1 (MQ=255)                                                                    aaaaaCCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCTGCCGCTTTTACAt                                                                              >  1:1213565/1‑149 (MQ=255)                                                                    aaaaaCCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACAt                                                                              >  1:1073660/1‑149 (MQ=255)                                                                              tCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCa                                                                   <  2:1121474/150‑1 (MQ=255)                                                                               cGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGCTCTGGTCAt                                                                  >  1:1227098/1‑150 (MQ=255)                                                                                ggTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATac                                                                <  1:785164/151‑1 (MQ=255)                                                                                      catcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCg                                                          >  2:1084374/1‑151 (MQ=255)                                                                                       atcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCg                                                          >  2:339759/1‑150 (MQ=255)                                                                                       atcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCg                                                          >  1:653901/1‑150 (MQ=255)                                                                                           ccAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGt                                                     <  2:550737/151‑1 (MQ=255)                                                                                           ccAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGt                                                     >  1:493142/1‑151 (MQ=255)                                                                                             aGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGc                                                   >  1:156983/1‑151 (MQ=255)                                                                                                      aTGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGc                                           >  1:1118637/1‑150 (MQ=255)                                                                                                           ggAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAgg                                     <  2:417505/151‑1 (MQ=255)                                                                                                             aaaaCTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCg                                   >  1:695367/1‑151 (MQ=255)                                                                                                                  tGTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATc                               >  2:222448/1‑150 (MQ=255)                                                                                                                   gTAAGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCAc                             >  1:664817/1‑151 (MQ=255)                                                                                                                     aaGCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACt                            <  2:1032717/150‑1 (MQ=255)                                                                                                                       gCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTc                          >  1:1091960/1‑150 (MQ=255)                                                                                                                       gCCCAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCa                         <  1:739741/151‑1 (MQ=255)                                                                                                                          cAGGCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAgtt                      >  1:872220/1‑151 (MQ=255)                                                                                                                             gCGAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAgttgtt                   <  1:85027/151‑1 (MQ=255)                                                                                                                               gAGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTgg                 >  2:4600/1‑151 (MQ=255)                                                                                                                                aGGAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgc                >  2:204121/1‑151 (MQ=255)                                                                                                                                 ggAATAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcg               <  1:742027/151‑1 (MQ=255)                                                                                                                                    aTAACCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCa             >  2:205359/1‑150 (MQ=255)                                                                                                                                      aaCCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAAcc          <  1:65228/151‑1 (MQ=255)                                                                                                                                      aaCCGGCGGTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAAc           <  1:184687/150‑1 (MQ=255)                                                                                                                                          ggcggTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACa      <  1:1143107/151‑1 (MQ=255)                                                                                                                                             ggTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAggg   <  1:1076987/151‑1 (MQ=255)                                                                                                                                              gTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGt  <  2:377148/151‑1 (MQ=255)                                                                                                                                              gTAACTCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGt  <  2:1060191/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGGAAATGGCTGCGTAATCCTGCTGGCGGATGGCGTTAATTAACCAGCGCCCTAAACCCGGCCAGCTAAAAACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGT  >  NC_000913/1354615‑1354906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑