Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	42.6%	92.8 / 16.2	61	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (7/28);  new base G (26/0);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 3.60e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354138‑354436                                                                                                                                                       |                                                                                                                                                     aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                                      <  2:535520/151‑1 (MQ=255)    ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGaa                                                                                                                                                   <  1:103195/151‑1 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGa                                                                                                                                        <  1:270524/151‑1 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTg                                                                                                                                         >  2:898098/1‑150 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGGGAGTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTGGGGGGGaga                                                                                                                                                  >  1:530871/1‑138 (MQ=255)                    tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCTGAAAggg                                                                                                                                   >  2:422022/1‑151 (MQ=255)                     gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGAATCGCTGGAAgg                                                                                                                                    >  2:273022/1‑149 (MQ=255)                      aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGGGAAAGATGCGCTGAAAggg                                                                                                                                   >  2:302648/1‑149 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                              <  1:367796/151‑1 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAggggg                                                                                                                            >  2:596908/1‑151 (MQ=255)                            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtgg                                                                                                                            <  2:450853/150‑1 (MQ=255)                              cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcg                                                                                                                          <  2:127776/150‑1 (MQ=255)                                       ctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGGGttt                                                                                                                 >  2:735152/1‑150 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTc                                                                                                              <  1:739780/151‑1 (MQ=255)                                           ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGGTTGTCAt                                                                                                            >  2:837692/1‑151 (MQ=255)                                            gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCAtt                                                                                                           <  1:584298/151‑1 (MQ=255)                                              gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTcc                                                                                                         <  2:874822/151‑1 (MQ=255)                                              gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAAGGCAGGGGGCGGTGGGGGTTGTCATTcc                                                                                                         >  2:916680/1‑151 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCg                                                                                                        <  2:204554/151‑1 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCg                                                                                                        <  1:597345/151‑1 (MQ=255)                                                 cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCg                                                                                                        >  2:135236/1‑149 (MQ=255)                                                  tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCCTTTCGaa                                                                                                      >  1:536786/1‑150 (MQ=255)                                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAaga                                                                                                   <  2:455767/151‑1 (MQ=255)                                                     cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGaaa                                                                                                     >  2:713524/1‑148 (MQ=255)                                                      gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTGGGGGGGAAAGATGCGCTGTAATGGGGGGGGGGGGTGGCGttttttcgttcgaa                                                                                                      >  1:217027/1‑135 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCg                                                                                              <  1:114717/151‑1 (MQ=255)                                                          tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGGAAGGGCAGGGGGCGGTGGCGGTTGTCATTTCGAAaaaga                                                                                                 >  1:607317/1‑147 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCCGAAAAGGGAGGTGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCg                                                                                              >  2:559950/1‑149 (MQ=255)                                                              tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGGTTGTCATTTCGaaa                                                                                                     >  1:964705/1‑139 (MQ=255)                                                               aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGGAAGAAGCGCCGAAAAGGGAGGTGGCGGGGGGGTTTGTCATTCCGTAagagaggg                                                                                              >  1:261863/1‑143 (MQ=255)                                                                aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                       <  1:1016971/151‑1 (MQ=255)                                                                aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGGAGCGCTGAAAGGGGAGGGGGGGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCt                                                                                       >  1:185449/1‑151 (MQ=255)                                                                 cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCGGGAAGGGGAGGGGGGGGGGGGGGTTGGCCTTTCGGAAGAGAGCGAAAGTCTg                                                                                      >  1:221181/1‑151 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTGGGGGGGCAGGAGCGCTGGAAGGGCAGGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGCGAccgggtgggag                                                                                  >  1:740138/1‑139 (MQ=255)                                                                        gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                               <  1:820880/151‑1 (MQ=255)                                                                         ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                              <  1:283454/151‑1 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                            >  2:373785/1‑147 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAt                                                                          >  1:845686/1‑149 (MQ=255)                                                                                gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGGGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGGtgtg                                                                       >  2:728563/1‑151 (MQ=255)                                                                                  tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTGTGGAAGATCGGGATGTgg                                                                      >  2:641408/1‑150 (MQ=255)                                                                                  tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGTTGGGGTGAAAGATGCGCTGGAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGTg                                                                            >  2:1046529/1‑144 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                                     <  2:477203/150‑1 (MQ=255)                                                                                    gggACGCGCGAGATTGAAGATAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCGGGTGGCGTTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgc                                                                     <  1:837473/149‑1 (MQ=255)                                                                                        cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                               <  2:695738/151‑1 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                            <  1:408893/151‑1 (MQ=255)                                                                                             gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAag                                                          <  2:237330/151‑1 (MQ=255)                                                                                             gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                            >  2:234926/1‑149 (MQ=255)                                                                                                     agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                  <  1:347430/151‑1 (MQ=255)                                                                                                       agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                                <  2:225587/151‑1 (MQ=255)                                                                                                       agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                                <  1:696380/151‑1 (MQ=255)                                                                                                       agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                  >  2:719974/1‑149 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTAt                                             <  1:894948/151‑1 (MQ=255)                                                                                                           tATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGCTGCGCTGAAAGGGGAGGGGGGGGGGGGGGTTGTCATTTCGAAAGAGAGCGACAGTTTGGAAGAGCGGGATGTGGGGCACCCGCAAGAGAAGGCGGTTAt                                             >  1:710824/1‑150 (MQ=255)                                                                                                                    ccATCCGGGCGTTGCCGAAGAGGCGGAGGCTGGGGTGAAAGGAGCGCCGAAAGGGCAGGTGGCAGGCGGGGTTGTCACTCCCAAAGAGAGCGACCGTCTGGAAGGACTCGATGGGGCGCGCTCGCAAGAGAAGGGGCTTCTGGCGCt                                       >  1:246434/1‑147 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                  >  2:96460/1‑150 (MQ=255)                                                                                                                       tCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtggggg                                  >  2:92415/1‑149 (MQ=255)                                                                                                                        ccGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTTATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                                <  1:773208/150‑1 (MQ=255)                                                                                                                          gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACa                               >  2:964584/1‑149 (MQ=255)                                                                                                                                tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                        <  2:320328/150‑1 (MQ=255)                                                                                                                                                  tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg     >  2:575901/1‑151 (MQ=255)                                                                                                                                                   ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg    <  1:995180/151‑1 (MQ=255)                                                                                                                                                     ggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgcg  <  1:441183/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                     ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGCG  >  NC_000913/354138‑354436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑