Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9170AC48.3% 80.5 / 21.2 58V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (27/3);  new base C (0/28);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.56e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGAT  >  NC_000913/993773‑994066
                                                                                                                                                  |                                                                                                                                                     
aGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                                  >  1:979072/1‑150 (MQ=255)
  aCGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGcc                                                                                                                                               >  2:210443/1‑151 (MQ=255)
        aTCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAAc                                                                                                                                         >  1:330348/1‑151 (MQ=255)
          ccagAAACATCATCCGTGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACACCGCGTGCGGTTGGCTTTCCCAGCCCTGCCAGCAGCCGCGGCGGGGTGTTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACt                                                                                                                                        <  1:944645/147‑1 (MQ=255)
          ccTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTg                                                                                                                                       >  1:805932/1‑151 (MQ=255)
                  atcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCg                                                                                                                               >  1:729106/1‑151 (MQ=255)
                    catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                              >  1:1046779/1‑150 (MQ=255)
                    catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                              >  2:609257/1‑150 (MQ=255)
                    catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                              >  2:367708/1‑150 (MQ=255)
                        cgcgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAatat                                                                                                                         >  1:79782/1‑151 (MQ=255)
                                ttCCTAATTTTCAGCCGGGGGTGTGGTGCCCGCTACCACACCCCCTGCGGTTGGCTTTCCCAGCCCTCCCAGCAGGCGCAGCGGGGTTCTTTTCCCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAat                                                                                                                           <  2:181024/141‑1 (MQ=255)
                                      attttCAGCCGGGGGTGTGGGCCCCGCTAACACACCGCCGGGGGTGGGCTTTCCCAGCCCTGCCAGCAGGCGCAGCAGGTTCTTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTgg                                                                                                           <  2:308976/149‑1 (MQ=255)
                                      atttccagccaggGTTGTGGCCCCCGCAAACCCACCGCCGGGGTTTGGTTTTCCCAGCCCTCCCAGCGGGCGCAGCGGGGTCTTTTTCCCCCCCCCGCTGCGCCCCACCACCGCCACAAACTGACCTGCCGGAatat                                                                                                                         <  2:635161/132‑1 (MQ=255)
                                         ttCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                        >  1:582016/1‑151 (MQ=255)
                                               cagggggggggtgCCCGCAACCACAGCCCCTGCGTTTGGCTTTTCCAGACCTGCCGGCGGGCGCAGCAGGGTTCTTTTCCCCCCCCCGCGGCCCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                                  <  2:549945/142‑1 (MQ=255)
                                                agcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                                  >  2:669405/1‑150 (MQ=255)
                                                 ggggTGGGGTCCCCGCAACCACTCCGCTTGGGTTTGGCTTTCCCAGACCGGCCGGCAGGCGCAGAGGGTTTTTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAg                                                                                                      <  1:758101/143‑1 (MQ=255)
                                                   ggTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGatat                                                                                              >  2:838345/1‑151 (MQ=255)
                                                   ggTGTGGTCCCGGCTACCACTCCGCTGGGGGTGGGCTTTCCAAGACCCCCGGGAAGGCGCAGCAGGTTCTTTTTCCACCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGatat                                                                                              <  2:881263/151‑1 (MQ=255)
                                                      gtgggccccGCAAACCCCCCGCCTGGGGTTGGCTTTCCCAGCCCTGCCGGCGGGCGCAGAGGGTTCTTTTCCCCCCCCCCGCGGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAttt                                                                                            <  1:396563/144‑1 (MQ=255)
                                                        ggtgCCCGCTAACACACCGCCTGCGTTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCGGGTTATTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTc                                                                                          <  2:47495/150‑1 (MQ=255)
                                                          tgCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGAACTGCCAGCAGGCGCAGCAGGGTAATTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcg                                                                                        >  1:938179/1‑150 (MQ=255)
                                                            cccccaaaCCCACCGCCGGCGGTGGGCTTTTCCAGCCCCGCCAGCAGGCGCGGCGGGGTTCTTTGCCCACCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcgc                                                                                       <  2:430860/143‑1 (MQ=255)
                                                            cccGCTAACACACCGCCTGCGGTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCGGGGTTTTTTTGCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCcgcg                                                                                      <  2:408424/150‑1 (MQ=255)
                                                               gCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAAt                                                                                  >  2:417424/1‑151 (MQ=255)
                                                                  accacaCCGCCGGCGGTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCGGGTTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATg                                                                                 <  2:361723/147‑1 (MQ=255)
                                                                   acacATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGttt                                                                              >  2:971513/1‑151 (MQ=255)
                                                                      caTCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCt                                                                           >  2:404192/1‑151 (MQ=255)
                                                                         cGCCTGCGGTTGGTTTTCCCAGCCCGGCCGGCAGGCGCGGCGGGTTTTTTTCCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                                          <  2:184257/149‑1 (MQ=255)
                                                                            cTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                     >  2:138379/1‑151 (MQ=255)
                                                                            cTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                     >  2:666839/1‑151 (MQ=255)
                                                                              gCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAt                                                                   >  1:376721/1‑151 (MQ=255)
                                                                               cGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAtt                                                                  >  2:525557/1‑151 (MQ=255)
                                                                                ggTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                                 >  2:658596/1‑151 (MQ=255)
                                                                                  ttGGCGTTCCCGGCCCGCCCCGCGGGCGCAGAGGGTTCTTTTGGCCCCCCCCGTGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTa                                                                         <  1:229057/141‑1 (MQ=255)
                                                                                    gtttttcccaccccTCCCCGCGGGCGCAGCGGGTTGTTTTCCCCCCCCCCGCGGCCCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGc                                                              <  1:721459/138‑1 (MQ=255)
                                                                                    ggCTTTCCCAGCCCTGCCAGCGGGCGCGGCGGGTTTCTTTTGCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                                                               <  2:93991/149‑1 (MQ=255)
                                                                                    gctttccccaccccTCCCAGCGGGCCCAGCGGGTTCCTTTCCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                                 <  1:627830/135‑1 (MQ=255)
                                                                                     gCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGca                                                             >  1:63396/1‑150 (MQ=255)
                                                                                       gTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaac                                                           >  1:499710/1‑150 (MQ=255)
                                                                                        ttcccagcccccccAGCGGGCGCAGCAGGTTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                                 <  1:747457/131‑1 (MQ=255)
                                                                                        ttcccaccccggCCGGCGGGCGCGGCAGGGTTTTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAg                                                               <  2:894490/134‑1 (MQ=255)
                                                                                           ccAGCCCTGCCGGCAGGCGCAGCAGGAATTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAAt                                                        <  2:345999/149‑1 (MQ=255)
                                                                                                ccTCCCAGCAGGCGCAGCGGGTTACTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGc                                                 <  2:1035392/151‑1 (MQ=255)
                                                                                                       gcagGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGtt                                          >  2:537304/1‑151 (MQ=255)
                                                                                                          ggCGCAGCGGGTTATTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCa                                        <  2:60862/150‑1 (MQ=255)
                                                                                                             gcagcaGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                                    >  2:524828/1‑151 (MQ=255)
                                                                                                              cagcagGGTCTTTTTGCCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                                    <  2:80903/150‑1 (MQ=255)
                                                                                                              cagcagGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACg                                    >  1:153624/1‑150 (MQ=255)
                                                                                                                gcggggTATTTTCCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAg                                  <  2:219746/147‑1 (MQ=255)
                                                                                                                   ggtttcTTTTCCCACCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCa                                <  2:1042122/144‑1 (MQ=255)
                                                                                                                    ggTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt                              >  1:237387/1‑150 (MQ=255)
                                                                                                                         ttttCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAt                            <  1:158512/147‑1 (MQ=255)
                                                                                                                          tttCCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCa                         <  2:294602/149‑1 (MQ=255)
                                                                                                                            tcgCCCCCACCGCGGCGTCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATa                       <  2:806055/148‑1 (MQ=255)
                                                                                                                                ccccccccGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGt                   <  1:643541/145‑1 (MQ=255)
                                                                                                                                  ccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTgg                 <  1:368180/147‑1 (MQ=255)
                                                                                                                                             ggCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCtt          <  2:303805/147‑1 (MQ=255)
                                                                                                                                              gCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt     >  2:24001/1‑151 (MQ=255)
                                                                                                                                               ccccccccCGCCACAAACGGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTc         <  1:461522/146‑1 (MQ=255)
                                                                                                                                                 caccacCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGAt  <  2:828997/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGAT  >  NC_000913/993773‑994066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: