Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	20,771	C→A	intergenic (‑263/+44)	insA ← / ← rpsT	IS1 repressor TnpA/30S ribosomal subunit protein S20

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	20,771	0	C	A	100.0%	278.6 / NA	72	intergenic (‑263/+44)	insA/rpsT	IS1 repressor TnpA/30S ribosomal subunit protein S20
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (33/39);  total (33/39)

TCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTG  >  NC_000913/20627‑20916                                                                                                                                                 |                                                                                                                                                  tCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCtt                                                                                                                                             >  2:163795/1‑151 (MQ=255)  cTTTTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTGCAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCg                                                                                                                                                <  1:483477/147‑1 (MQ=255)  cTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCtt                                                                                                                                             >  1:566447/1‑150 (MQ=255)    tGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTgc                                                                                                                                           <  2:57207/150‑1 (MQ=255)            aTGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCttt                                                                                                                                  <  2:185974/151‑1 (MQ=255)              gTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCttttt                                                                                                                                >  2:774847/1‑151 (MQ=255)              gTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCttttt                                                                                                                                <  1:586080/151‑1 (MQ=255)              gTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCtttt                                                                                                                                 <  2:721292/150‑1 (MQ=255)                      cGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGc                                                                                                                        >  1:772606/1‑151 (MQ=255)                      cGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGc                                                                                                                        >  2:622553/1‑151 (MQ=255)                       gATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCt                                                                                                                       >  2:671609/1‑151 (MQ=255)                       gATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCt                                                                                                                       >  2:679340/1‑151 (MQ=255)                        aTGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCtt                                                                                                                      <  2:465533/151‑1 (MQ=255)                          ggTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCa                                                                                                                    <  1:80280/151‑1 (MQ=255)                          ggTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCa                                                                                                                    <  2:835021/151‑1 (MQ=255)                           gTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTc                                                                                                                     >  1:128669/1‑149 (MQ=255)                               tACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCaa                                                                                                                >  1:724321/1‑150 (MQ=255)                                aCGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCa                                                                                                                 <  2:752735/148‑1 (MQ=255)                                 cGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAAtt                                                                                                             <  1:682237/151‑1 (MQ=255)                                 cGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAAtt                                                                                                             <  2:515547/151‑1 (MQ=255)                                  gATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTTACAAAGGTTCAGCAAATTg                                                                                                            >  2:911200/1‑151 (MQ=255)                                  gATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTg                                                                                                            >  1:143015/1‑151 (MQ=255)                                   aTTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTgg                                                                                                           >  1:993475/1‑151 (MQ=255)                                   aTTCGTAAGCCACGAAGAGTTCAGATAGTCCAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCTCAAAGCTTCAGCAAATTgg                                                                                                           <  1:136904/151‑1 (MQ=255)                                     tCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCg                                                                                                         >  1:525644/1‑151 (MQ=255)                                        tAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCg                                                                                                         <  2:694351/148‑1 (MQ=255)                                        tAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGa                                                                                                        <  1:283194/149‑1 (MQ=255)                                        tAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGAt                                                                                                       <  1:689077/150‑1 (MQ=255)                                         aaGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGAtt                                                                                                      >  1:145577/1‑150 (MQ=255)                                         aaGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCACACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGAtt                                                                                                      >  1:493057/1‑150 (MQ=255)                                               cGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGcc                                                                                                 >  2:153331/1‑149 (MQ=255)                                                 aaGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGtt                                                                                             >  1:840737/1‑151 (MQ=255)                                                  agagTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGttt                                                                                            >  2:447739/1‑151 (MQ=255)                                                  agagTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGttt                                                                                            <  2:57710/151‑1 (MQ=255)                                                   gagTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTttg                                                                                           <  2:692436/151‑1 (MQ=255)                                                   gagTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTttg                                                                                           <  2:132168/151‑1 (MQ=255)                                                    agTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTttg                                                                                           <  2:966084/150‑1 (MQ=255)                                                         aGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATc                                                                                     >  1:343891/1‑151 (MQ=255)                                                          gATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATc                                                                                     <  2:873558/150‑1 (MQ=255)                                                                 cAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGt                                                                             <  2:583185/151‑1 (MQ=255)                                                                   aCGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCa                                                                           <  1:432497/151‑1 (MQ=255)                                                                    cGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTc                                                                            >  1:879750/1‑149 (MQ=255)                                                                     ggCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAgg                                                                         <  1:435848/151‑1 (MQ=255)                                                                        aTGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGtt                                                                       <  1:631590/150‑1 (MQ=255)                                                                        aTGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTa                                                                      <  1:591919/151‑1 (MQ=255)                                                                        aTGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGAGCTGTGCAGTCAGGTTa                                                                      <  2:363674/151‑1 (MQ=255)                                                                                     cTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTg                                                         >  2:618195/1‑151 (MQ=255)                                                                                        tCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGc                                                        >  2:262657/1‑149 (MQ=255)                                                                                           ggCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCt                                                    <  2:254462/150‑1 (MQ=255)                                                                                              aaCCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGt                                                <  1:964198/151‑1 (MQ=255)                                                                                                 cGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGtttt                                             >  1:1026329/1‑151 (MQ=255)                                                                                                    cAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTtgt                                          >  1:188764/1‑151 (MQ=255)                                                                                                     aGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTtgtg                                         <  1:595338/151‑1 (MQ=255)                                                                                                        gtgtTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGAt                                      >  2:741004/1‑151 (MQ=255)                                                                                                        gtgtTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGAt                                      <  2:664632/151‑1 (MQ=255)                                                                                                          gtTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATc                                     >  1:919049/1‑150 (MQ=255)                                                                                                                tcACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCtt                              >  1:469861/1‑151 (MQ=255)                                                                                                                 cACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGTGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCttt                             >  2:732829/1‑151 (MQ=255)                                                                                                                 cACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCttt                             <  2:829545/151‑1 (MQ=255)                                                                                                                 cACGCATCACAAAAGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCtt                              <  2:803695/150‑1 (MQ=255)                                                                                                                            aaaGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGAc                  >  1:221516/1‑151 (MQ=255)                                                                                                                            aaaGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGAc                  <  2:117893/151‑1 (MQ=255)                                                                                                                             aaGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACg                 <  2:219249/151‑1 (MQ=255)                                                                                                                             aaGCAGCAGGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACg                 >  1:99212/1‑151 (MQ=255)                                                                                                                                cagcagGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTTAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTc              <  1:36106/151‑1 (MQ=255)                                                                                                                                cagcagGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTc              >  2:944294/1‑151 (MQ=255)                                                                                                                                  gcagGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCa            <  1:857090/151‑1 (MQ=255)                                                                                                                                    agGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACg          <  2:189475/151‑1 (MQ=255)                                                                                                                                    agGCATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACg          >  1:961952/1‑151 (MQ=255)                                                                                                                                       cATAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATc       <  1:592797/151‑1 (MQ=255)                                                                                                                                        aTAAAAAAAACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCg      <  2:940855/151‑1 (MQ=255)                                                                                                                                           aaaaaaACCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGtt   >  1:936325/1‑151 (MQ=255)                                                                                                                                            aaaaaaCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTg  <  2:566547/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  TCTTGTTCAGAATGTATCAGCCGATGGTTCTACGATTCTTAAGCCACGAAGAGTTCAGATAGTACAACGGCATGTCTCTTTTGACTATCTGGCAACCGGCAGTGTGTTCTCTCACGCATCACAAAAGCAGCAGGCATAAAAAAACCCGCTTGCGCGGGCTTTTTCACAAAGCTTCAGCAAATTGGCGATTAAGCCAGTTTGTTGATCTGTGCAGTCAGGTTAGCCTTATGACGTGCAGCTTTGTTTTTGTGGATCAGACCTTTAGCAGCCTGACGGTCCACGATCGGTTG  >  NC_000913/20627‑20916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑