Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,480,371	0	T	G	40.7%	143.4 / 12.7	81	G390G (GGT→GGG)	dsdA	D‑serine dehydratase
Reads supporting (aligned to +/- strand):  ref base T (8/40);  new base G (33/0);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 2.12e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGGTCGCGCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTGGCA  >  NC_000913/2480225‑2480518                                                                                                                                                   |                                                                                                                                                      gTTGGTCGCGCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATgg                                                                                                                                                   <  1:245171/151‑1 (MQ=255)   tGGTCGCGCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGc                                                                                                                                                  <  2:377929/150‑1 (MQ=255)    ggTCGCGCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGcc                                                                                                                                                 <  2:979461/150‑1 (MQ=255)      tCGCGCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATggg                                                                                                                                                  >  1:777077/1‑146 (MQ=255)       cgcgCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGAc                                                                                                                                             <  1:554258/151‑1 (MQ=255)              cAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAgc                                                                                                                                       <  1:487206/150‑1 (MQ=255)               aGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGccccccc                                                                                                                                       >  2:575831/1‑144 (MQ=255)                ggCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCCCAgc                                                                                                                                       >  1:1047694/1‑148 (MQ=255)                        cgggcgggCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCCCCGCGCGggtgtg                                                                                                                              >  2:543420/1‑149 (MQ=255)                         gggcgggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCa                                                                                                                            <  1:111920/150‑1 (MQ=255)                         gggcgggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCAt                                                                                                                           <  1:492446/151‑1 (MQ=255)                         gggcgggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCCGCGCGcgtgtg                                                                                                                              >  2:1157193/1‑148 (MQ=255)                         gggagcgcAATGGAGCGTCTGCTGGATGGCGTTTATACCCTTAGCGATCAAACCATGTATGAAATGCTTGGCGGGCTGGCGCATGAAGAAGGTATTCGTCTTGAACCTACGGCACTGGCGGGTATGGCCGGACCTCAGCGCgtgtat                                                                                                                               <  1:369281/141‑3 (MQ=255)                          ggcgggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATc                                                                                                                          <  2:1128742/151‑1 (MQ=255)                            cgggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGGCGGAACTCCCCGCgcgtg                                                                                                                                >  2:250133/1‑143 (MQ=255)                             gggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGACGGGGCCCACCGCGTGGGTGGATCAg                                                                                                                        >  1:1016462/1‑150 (MQ=255)                              ggcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑a                                                                                                                     <  1:1134546/151‑1 (MQ=255)                               gcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑aa                                                                                                                    <  1:194774/151‑1 (MQ=255)                               gcAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑aa                                                                                                                    <  1:1242822/151‑1 (MQ=255)                                   tGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCTCCCCGCCGGGGTGCATCCGC‑AAg                                                                                                                   >  1:1213713/1‑148 (MQ=255)                                    ggAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGGTGGGCGGGCCCCCGCGCGGGGGGGCATCAGT‑AAGtt                                                                                                                 >  1:1283957/1‑149 (MQ=255)                                    ggAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGACCTCCGCGCGGGTGTGCATCAGT‑AAGTTa                                                                                                                >  2:385385/1‑150 (MQ=255)                                     gAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAc‑c                                                                                                             <  2:796753/151‑1 (MQ=255)                                      aGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑Ca                                                                                                            <  1:761400/151‑1 (MQ=255)                                        cGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAAc                                                                                                          <  1:822586/151‑1 (MQ=255)                                         gTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACa                                                                                                         <  1:490869/151‑1 (MQ=255)                                             gctgGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGa                                                                                                       <  1:1123326/149‑1 (MQ=255)                                               tgGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGATATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCTCCGCGCGGGGGTGCATCAGG‑AAGGTAC‑CAACAAAt                                                                                                      >  1:997505/1‑148 (MQ=255)                                                ggATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCa                                                                                                   <  1:518878/150‑1 (MQ=255)                                                ggATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCa                                                                                                   <  1:317937/150‑1 (MQ=255)                                                ggATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGACCCCCGCGCGGGGGTGGATCAGT‑AAGTTAA‑CAACAGATGCAc                                                                                                  >  1:407242/1‑151 (MQ=255)                                                     gCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCAACGCGTGTGTGCATCAGT‑AAGTTAC‑CCACAGATGTACGGttt                                                                                             >  1:380324/1‑151 (MQ=255)                                                        tCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCCCCGCGCGGGTGTGCCTCAGT‑AAGGTAC‑CCAAAGATGCACGGgtt                                                                                             >  2:499221/1‑148 (MQ=255)                                                        tCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGACCCCAGCGCGGGTGGGCATCCGG‑AAGGTAA‑CAACAAATGCCCGGgtt                                                                                             >  1:695903/1‑148 (MQ=255)                                                           ataCCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCCGCGCGGGGGTGGCTCCGG‑AAGGTAC‑CAACAGATGCCCGGGTTTCgcgc                                                                                       >  1:568694/1‑151 (MQ=255)                                                              cccTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGGCCCCCGCGCGGGTGTGCCTCAGT‑AAGTTAC‑CAAAAGATGCACGGgtt                                                                                             >  2:871997/1‑142 (MQ=255)                                                              cccTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGa                                                                                    <  2:769927/151‑1 (MQ=255)                                                                   aGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGGCTCAGACCGGGGGGGCGTCCGT‑AAAGTAT‑CAACAAAAGAACGGGTTCAGCGCGGaaca                                                                                 >  1:231919/1‑149 (MQ=255)                                                                   aGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCCGCGCGTGGGTGGATCCGG‑AAGTTAC‑CAACAGATGCACGGGTTCAGCGCCGaacaa                                                                                >  1:1078267/1‑150 (MQ=255)                                                                       aTCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCAGCGCGTGTGTGGATCAGT‑AAGATAA‑CAACAAATGCCCGGGTTTAGCGCAGAACAAATGc                                                                            >  2:908810/1‑150 (MQ=255)                                                                        tCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCTCCCCGCGCGTGTGCATCAGT‑AAGTTAC‑CAACAGAAGCACGGGTTCAGCGCCGAAAAACTGCg                                                                           >  2:1468/1‑150 (MQ=255)                                                                        tCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGGCCCCGCGCGGGTGGGGATCAGT‑AAGATAA‑CAAAAAAAGCACGGGTTTCGCGCAGaaaaa                                                                                >  1:1088957/1‑145 (MQ=255)                                                                        tCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCAGCGCGGGTGTGGATCAGG‑AAGTTAA‑CAACAGATGCACGGGTTCAGCGCCGaacaaacgcg                                                                           >  1:445789/1‑145 (MQ=255)                                                                         cAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCTCAGCGCGGGTGTGCATCCGT‑AAGTTAC‑CCACAGAAGCACGGGTTTAGCGCAGAACAAAAGCGt                                                                          >  2:1143880/1‑150 (MQ=255)                                                                              cATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATAcc                                                                    <  1:50573/151‑1 (MQ=255)                                                                                     gACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGGCCTCACCGCGGGGGGGGATCAGT‑AAGTTAA‑CAACAGAAGCACGGGTTCAGCGCAGAACAACAGCGGAATACCACTc                                                                >  1:1213044/1‑148 (MQ=255)                                                                                       cATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATTGCCGGACCCCCGCGCGGGTGTGCATCAGT‑AAGTTAA‑CAACAGATGCACGGGTTTAGCGCAGAAAAAACGCGTAATAACACTCa                                                               >  2:517333/1‑147 (MQ=255)                                                                                       cATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGACCTCCACGCGCGTGTGGATCAGT‑AAGTTAA‑CAACAGATGCACGGGTTCAGCGCAGAACAACTGCGGAATACCACTc                                                                >  2:1024340/1‑146 (MQ=255)                                                                                        aTGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGGCGGGCCTCCGCGCGGGTGTGCATCAGT‑AAGATAA‑CCACAGATGCACGGGTTTAGCGCAGAAAAACAGCGGAATAACACCCATCTgg                                                          >  2:128442/1‑151 (MQ=255)                                                                                         tGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGACCTCCGCGCGTGTGTGGATCAGG‑AAGGTAA‑CAACAAAAGCCCGGGTTCTGCGCAGAAAAAATGCGTAATAACACTCATCTgg                                                          >  2:567421/1‑150 (MQ=255)                                                                                          gCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGgt                                                         <  2:109129/150‑1 (MQ=255)                                                                                            ttGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGACCCCAGCGCGGGTGTGCATCAGT‑AAGTTAC‑CAAAAAAAGCACGGGTTCAGCGCAGAACAAATGCGGAAAACCACTCATCTGgtg                                                        >  2:403579/1‑149 (MQ=255)                                                                                               gctggctggcGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGc                                                   <  2:383287/151‑1 (MQ=255)                                                                                                  ggctggcGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGAc                                                <  1:265942/151‑1 (MQ=255)                                                                                                      ggcGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACggg                                             <  2:788962/150‑1 (MQ=255)                                                                                                      ggcGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGa                                            <  2:578295/151‑1 (MQ=255)                                                                                                      ggcGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGa                                            <  2:451291/151‑1 (MQ=255)                                                                                                          cAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATTGCCGGACCTCAGCGCGTGTGTGGGTCAGT‑AAGTTAA‑CAAAAGATGGACGGGTTTAGCGCAGAACAAATGCGTAATACCACTCCTCTGGGGTGGGGGGCgggggggg                                        >  2:1124524/1‑149 (MQ=255)                                                                                                          cAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGGCGGACCTCCGCGCGGGGGTGCATCAGT‑AAGGTAC‑CAACAAAAGCACGGGTTCAGCGCCGAACAAATGCGGAAAACCACCCCTCTGGTGTGGGGGGCgggggggg                                        >  2:1289134/1‑149 (MQ=255)                                                                                                           aGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGACCTCAGCGCGTGTGGGGATCAGT‑AAGGTAA‑CAACAAAAGCACCGGTTTAGCGCAGAACAACTGCGTAAAACCACCCCTCTTGGGGGGGGGGCGgggggggg                                       >  1:471544/1‑151 (MQ=255)                                                                                                            ggAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGa                                      <  1:645165/151‑1 (MQ=255)                                                                                                               agaagGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATg                                   <  1:502824/151‑1 (MQ=255)                                                                                                               agaagGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCGGGCCCCAGCGCGGGGGGGGATCAGT‑AAATTAA‑CAACAGATGCACGGGTTTTGCGCAGAACAAACGCGTAAAACCACCCATCTGGTGTGGGGGGCGggggggggga                                     >  1:641413/1‑147 (MQ=255)                                                                                                               agaagGTATTCGTCTTGAACCTTCGGCACTGGCGGGGATGGCCCGATCGCAGGGCGTGTTTGCATCAGC‑AAGTTACACAAC‑GAGTCACGGGGTCCGCGCAGAAAAAAAGCGTAATAACAATCATCTGGTGTGGGGGGAGGGAGGGGGAGTg                                   >  1:137971/1‑151 (MQ=255)                                                                                                                gaagGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATgg                                  <  1:1021474/151‑1 (MQ=255)                                                                                                                      aTTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCg                             <  2:855382/150‑1 (MQ=255)                                                                                                                        tCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGga                           <  1:472701/150‑1 (MQ=255)                                                                                                                         cGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGgaa                          <  1:405757/150‑1 (MQ=255)                                                                                                                          gTCTTGAACCTTCGGCACTGGCGGGTATGGGCGGGCCTCAGCGCGTGTGTGCCTCAGT‑AAGTTAA‑CAACAAATGCACGGGTTTCGCGCAGAACAAATGCGTAATAACACTCATCTGGTGTGGGCGGCGGGGGGGGGGAAGGGGCCg                             >  2:1199883/1‑146 (MQ=255)                                                                                                                            cTTGAACCTTCGGCACTGGCGGGTATGGGCGGGCCCCCGCGCGGGTGGGCATCAGTAAAGTAAC‑CACCA‑ATGGACGGGTTTCGCGCCGAACAAATGCGGTATACCACTCCACTTGGGTGGGGGACGGGAGGGGGGATGGTGGCGggagga                       >  2:1224725/1‑148 (MQ=255)                                                                                                                            cTTGAACCTTCGGCACTGGCGGGGAAGGGCGGGCCTCAACGCGGGTGTGCATCAGT‑AAAGTAC‑CAACAGATGCACGGGTTCTGCGCCGAACAACAGCGTAATACCACTCATCTGGTGTGGGGGGCGGGGGGGGGGATGGGGCCGggagaa                       >  1:565387/1‑150 (MQ=255)                                                                                                                                  ccTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGa                <  2:295412/151‑1 (MQ=255)                                                                                                                                  ccTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGa                <  2:1284119/151‑1 (MQ=255)                                                                                                                                     tCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGaa               <  2:1030349/149‑1 (MQ=255)                                                                                                                                     tCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAAt              <  2:645143/150‑1 (MQ=255)                                                                                                                                         cACTGGCGGGTATGGCCCGACCTCCGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGAAGGACGGGTTCAGCGCAGGACAACTGCGGAATACCACTCATCTGGTGTGGGGGACGGGGGGGGGGAAGGGGGCGGGAGAAGAGATGAAACaa           >  1:100940/1‑149 (MQ=255)                                                                                                                                             ggCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTg     <  1:913857/151‑1 (MQ=255)                                                                                                                                             ggCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTg     <  2:144204/151‑1 (MQ=255)                                                                                                                                              gCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTgg    <  2:395675/151‑1 (MQ=255)                                                                                                                                              gCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTgg    >  1:934374/1‑151 (MQ=255)                                                                                                                                                 ggTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTGGCa  >  2:431783/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GTTGGTCGCGCATCAGGCTTTGTCGGGCGGGCAATGGAGCGTCTGCTGGATGGCTTCTATACCCTTAGCGATCAAACCATGTATGACATGCTTGGCTGGCTGGCGCAGGAAGAAGGTATTCGTCTTGAACCTTCGGCACTGGCGGGTATGGCCGGACCTCAGCGCGTGTGTGCATCAGT‑AAGTTAC‑CAACAGATGCACGGTTTCAGCGCAGAACAACTGCGTAATACCACTCATCTGGTGTGGGCGACGGGAGGTGGAATGGTGCCGGAAGAAGAGATGAATCAATATCTGGCA  >  NC_000913/2480225‑2480518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑