Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,757	0	A	C	40.7%	147.1 / 19.6	81	G150G (GGT→GGG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base A (44/4);  new base C (0/33);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 1.23e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATCGAGGTAGCAGTC  >  NC_000913/3035608‑3035903                                                                                                                                                          |                                                                                                                                                   tCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATAc                                                                                                                                                   >  1:1237467/1‑151 (MQ=255)  ccGGATAAACAAACGCGGAGCTGAACGGCGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATAcc                                                                                                                                                  <  1:647304/151‑1 (MQ=255)  ccGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATAcc                                                                                                                                                  >  1:1154204/1‑151 (MQ=255)   cGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATAccc                                                                                                                                                 <  2:1059807/151‑1 (MQ=255)     gATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGc                                                                                                                                               <  1:753556/151‑1 (MQ=255)         aacaaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACt                                                                                                                                           >  1:200412/1‑151 (MQ=255)            aaacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTc                                                                                                                                         >  1:652689/1‑150 (MQ=255)             aacGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCAc                                                                                                                                       >  2:318434/1‑151 (MQ=255)                  ggAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGCTTT‑AATACCCGCCACTTCACCTTcc                                                                                                                                  >  1:427913/1‑151 (MQ=255)                     gCTGAACGCGGTGGGGCGCCGCCCGCGGGAGTAAAAGGGGTTTTTCCGCCCCGGGCGGTGACCGCCGG‑TTTTTGTACGCACCCAGCCGTAA‑GCGTAACCGC‑GGGAGTTTTTGATCGTCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGa                                                                                                                                <  2:1094629/150‑1 (MQ=255)                      cTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGAc                                                                                                                               >  1:931648/1‑150 (MQ=255)                       tGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGActc                                                                                                                             >  2:1084776/1‑151 (MQ=255)                        gAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGActct                                                                                                                            >  2:698291/1‑151 (MQ=255)                         aaCGACGTGTGGCGACGACTGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCtt                                                                                                                           >  2:17588/1‑151 (MQ=255)                                 gtgGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGAtt                                                                                                                   >  1:1141186/1‑151 (MQ=255)                                 gtgGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGAtt                                                                                                                   >  1:292237/1‑151 (MQ=255)                                   ggCGCCGCCCGCGGGAGACAACCGGGTTTTTACGC‑CCGGGGGGGGGACCCCGGTTTTCTGTCCGCCCCCAGCCGAAA‑GCTAAATCGC‑CGGAGTTTTTGATCGTCACGGTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTc                                                                                                                 <  2:582428/151‑1 (MQ=255)                                     cgaggaCCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGGGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTCA‑GCGTACTCGC‑CGGAGCTTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGAttt                                                                                                                  >  1:821777/1‑148 (MQ=255)                                      gacgacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGt                                                                                                              >  2:985417/1‑151 (MQ=255)                                       acgacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtt                                                                                                             >  2:998541/1‑151 (MQ=255)                                        cgacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGttt                                                                                                            >  1:396053/1‑151 (MQ=255)                                        cgacCGCCGGAGTAAACGGGGTTTTTCCACACGGGGGGTTGACCGCTGT‑TTTCTGTCCGCGGCCGGCGGTAG‑GCTAAACCGC‑GGGATTTTTTGTTCTTCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGttt                                                                                                            <  2:1033136/151‑1 (MQ=255)                                         gacCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                                                           >  2:2454/1‑151 (MQ=255)                                           ccgcggGAGTAAAACGGGTTTTTCCGCACGGGGGGGTGACCGCGGT‑TTTTTGTCCGCACCCAGCCGTAA‑GCTTAACCGC‑GGGAGTTTTTGATCGTCAGGGATTA‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa                                                                                                                <  2:1127195/144‑1 (MQ=255)                                           ccgccgGAGTAAACGGGGTTTTTACGCCCGGGGGGGTGACCCCCGT‑TTTTTGTCCGCACCCAGCCGTAA‑GCGTAACCGC‑CGGATTTTTTGACCGCCGCGTATTT‑ATTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                                                           <  2:392278/149‑1 (MQ=255)                                            cggcgGAATAAAACGGGTTTTTCCCCCCCGGGGGGGGAACCCCGG‑TTTCTGGACCCAGCCCGCCGAAA‑GGGTAACCCC‑GGGAATTTTTGATCGTCCGGGTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGtttt                                                                                                           <  2:847567/145‑1 (MQ=255)                                              cgggagcaagtggggCTTTGCCGCCCGGGGCGGTGACCTCCGG‑TTTCTGCCCGCAGCC‑GCCGAAA‑GGGTTATGGCGCGGGTTTTTTGTTCGTCATGATTTT‑ATTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAc                                                                                                      <  1:490732/139‑1 (MQ=255)                                              ccgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAc                                                                                                      >  2:1005216/1‑151 (MQ=255)                                               cgGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAcc                                                                                                     >  1:433729/1‑151 (MQ=255)                                                ggACTCAACCGGTTTTTCACCCTCCTGGCGGTGACCCCGGG‑TTTCTGCCCGCAGCCCGCCGGAG‑TCTTAACCGC‑CGGTGTTTTTGATCTCCAGGATTTT‑ATTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAcc                                                                                                     <  2:316729/150‑1 (MQ=255)                                                 gAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAc                                                                                                   >  1:953012/1‑151 (MQ=255)                                                     cAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgcg                                                                                                >  1:296684/1‑150 (MQ=255)                                                       agcggctttttCCGCCCCGGGGGGTGACCGCGGT‑TTTCTGGCCGCCCCCAGCCGTAG‑GCGTAATCGC‑CGGAGTTTTTGTTGTTCTGGAATTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcg                                                                                                  <  1:353832/139‑1 (MQ=255)                                                          gggTTTTTGCCCCCCGGGGGGTGAACCCCGT‑TTTCTGCCCGCGCCCAGCCGTAA‑GCGTACTCGC‑GGGATTTTTTGATCGTCAGGGTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCt                                                                                           <  1:640285/150‑1 (MQ=255)                                                          gggTTTTTACCCACGGGGGGGTGACCGCGGT‑TTTCTGCACGCCGCCAGCCGTAA‑GCGTAATCGC‑GGGATTTTTTGATCGCCGCGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAc                                                                                                   <  1:174930/142‑1 (MQ=255)                                                           gttttttCCGCCCCGGGGGGGGACCGCGGT‑TTTTTGTCCGCGGCCGGCCGTAA‑GCTTAATCGC‑GGGAGTTTTTGATCGCCACGGTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGc                                                                                         <  2:816370/148‑1 (MQ=255)                                                                     cACCGGGCGGAGACCGCCTT‑TTTCTGTCCCCACCCAG‑CGTAAGGCTTAACCGC‑CGGATATTTTGATCGTCACGTTTTT‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGa                                                                               <  1:137473/151‑1 (MQ=255)                                                                     cACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc                                                                                  >  2:1180472/1‑148 (MQ=255)                                                                       ccggggggtaaccGCCGT‑TTTTTGCACGCACCCAGCCGTAA‑GCGTAATCGC‑CGGAGTTTTTGACCGTCCCGAATTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGat                                                                              <  2:698308/138‑1 (MQ=255)                                                                       ccAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGat                                                                              >  2:917256/1‑150 (MQ=255)                                                                              gTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGc                                                                      >  1:737425/1‑151 (MQ=255)                                                                                 aaCGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTc                                                                    >  1:108788/1‑150 (MQ=255)                                                                                      cGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAt                                                              >  2:1137237/1‑151 (MQ=255)                                                                                      cGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAt                                                              >  1:552034/1‑151 (MQ=255)                                                                                        g‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGc                                                            >  2:244434/1‑151 (MQ=255)                                                                                          tttCTGTCCGCACCCCGCCGTAG‑GCTTAATCGC‑CGGAGTTTTTGTTCGTCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAg                                                                 <  2:1284726/145‑1 (MQ=255)                                                                                          tttCTGTACGCGCCCAGCCGAAA‑GCGAAACCGC‑GGGAGTTTTTGATCGTCAGGGATTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCAt                                                              <  2:727017/148‑1 (MQ=255)                                                                                           ttCTGTCCGCAGCCAGCCTTAA‑GCGTAACCGC‑GGGATTTTTTGATCGCCACGTATTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCt                                                           <  2:1269847/150‑1 (MQ=255)                                                                                              tGTCCGCACCCAGCCGAAG‑GCGTAATCGC‑GGGAGATTTTGATCGTCACGGATTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTc                                                          <  1:1247370/148‑1 (MQ=255)                                                                                               gtccgcgGCCGGCCGTAA‑GCGTAACCGC‑GGGAGTTTTTGATCGCCACGTTTTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGcc                                                       <  2:273283/147‑1 (MQ=255)                                                                                               gtccccagccagcGGAAA‑GAGAAATCGC‑CGGATTTTTTGATCGTCACGGATTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCg                                                         <  2:208147/143‑1 (MQ=255)                                                                                                      gccagccGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCtg                                               >  1:1051270/1‑151 (MQ=255)                                                                                                         cggcggaagccttAACCGC‑AGGATTTTTTGTTCGCCTGGGTTTA‑AATCCCCGCCACTTCACCTTCCGGCTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAg                                                    <  1:96245/132‑1 (MQ=255)                                                                                                         agccGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGc                                            >  2:805950/1‑151 (MQ=255)                                                                                                          gccGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCt                                           >  1:580787/1‑151 (MQ=255)                                                                                                           ccGAAA‑GCGTAACCGC‑CGGATTTTTTGATCGTCACGGATTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAg                                                    <  1:1220210/141‑1 (MQ=255)                                                                                                             gTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTcc                                        >  2:799479/1‑151 (MQ=255)                                                                                                              gaacgCGTCACCGC‑GGGAGATTTTGTTCTCCCCGGATTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTc                                         <  1:59484/146‑1 (MQ=255)                                                                                                                     tAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGc                                 >  1:1101659/1‑151 (MQ=255)                                                                                                                        accggcGGATATTTGGATCGCCA‑GAATTTAAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTc                                                  <  1:1117509/127‑1 (MQ=255)                                                                                                                        accgcgggattttttGCTCGTCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCa                               <  1:528199/140‑1 (MQ=255)                                                                                                                          gC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGAc                            >  2:630452/1‑151 (MQ=255)                                                                                                                           c‑cGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGAcc                           >  1:123746/1‑151 (MQ=255)                                                                                                                             gggattttttGCTCGTCTCGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGAccc                          <  1:748569/145‑1 (MQ=255)                                                                                                                              ggAGTTTTTGACCGCCAGGTTTTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACTACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGAcccc                         <  2:1106055/151‑1 (MQ=255)                                                                                                                              ggAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGAcccc                         >  1:1179138/1‑151 (MQ=255)                                                                                                                              ggAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCACCCGAcccc                         >  1:295855/1‑151 (MQ=255)                                                                                                                                agaTTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGc                       >  2:603753/1‑151 (MQ=255)                                                                                                                                agaTTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGc                       >  1:1013338/1‑151 (MQ=255)                                                                                                                                 gaTTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGcc                      >  1:1226981/1‑151 (MQ=255)                                                                                                                                   ttttTATCGTCCGGTTTTT‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGAcccc                         <  2:1275364/146‑1 (MQ=255)                                                                                                                                   ttttGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTg                    >  2:679232/1‑151 (MQ=255)                                                                                                                                    tttGACCGTCAGGAATTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTg                    <  1:917127/150‑1 (MQ=255)                                                                                                                                     ttgaccgaccgataTTT‑AAGCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGa                   <  2:191598/137‑1 (MQ=255)                                                                                                                                      tGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAat                 >  2:386395/1‑151 (MQ=255)                                                                                                                                        gttccgcccgATTTAAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCGGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGaa                  <  2:544673/140‑1 (MQ=255)                                                                                                                                         tCGTCACGGTTTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGGTTCTGCCCAGCGCGGAGACATACGCGCAATCATGCTCGCCCATTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATc              >  1:972302/1‑151 (MQ=255)                                                                                                                                              aCGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATCGAGGt         >  2:68757/1‑151 (MQ=255)                                                                                                                                                   ttt‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATCGAGGTAGCa     <  1:1100441/150‑1 (MQ=255)                                                                                                                                                     taaaTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATCGAGGTAGCAg    <  2:422824/148‑1 (MQ=255)                                                                                                                                                       aaTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATCGAGGTAGCAGTc  <  2:292287/150‑1 (MQ=255)                                                                                                                                                          |                                                                                                                                                   TCCGGATAAACAAACGCGGAGCTGAACGACGTGTGGCGACGACCGCCGGAGTCAAACGGGCTTTTACGCACCAGGCGGTGAACGCCGG‑TTTCTGTACGCAGCCAGCCGTAA‑GCGTAATCGC‑CGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGCATGCTCGCCCAGTCCTGTGCTTCCGTACCGCCAGACCCCGCCTGAATATCGAGGTAGCAGTC  >  NC_000913/3035608‑3035903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑