Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,112,578	0	T	G	42.3%	140.6 / 14.4	81	G88G (GGT→GGG)	yiiR	DUF805 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (5/40);  new base G (33/0);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.60e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAA  >  NC_000913/4112429‑4112724                                                                                                                                                       |                                                                                                                                                               aaGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGt                                                                                                                                                                <  2:180281/150‑1 (MQ=255) aaGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTa                                                                                                                                                               <  2:184724/151‑1 (MQ=255)         aCTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑g                                                                                                                                                         <  2:705413/147‑1 (MQ=255)         aCTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGCGGCGTGGATGCTGCTGGC‑GGGTa                                                                                                                                                               >  2:807475/1‑143 (MQ=255)         aCTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGACGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CT‑ggggg                                                                                                                                                     >  2:13247/1‑149 (MQ=255)          cTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑ggg                                                                                                                                                       >  2:77282/1‑148 (MQ=255)            cGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑Ga                                                                                                                                                   <  2:584527/149‑1 (MQ=255)            cGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CT‑ggggggtt                                                                                                                                                  >  1:1190984/1‑146 (MQ=255)                 ttCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CC‑GGGG‑GG‑ttttt                                                                                                                                             >  2:867886/1‑148 (MQ=255)                  tCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑ttttttc                                                                                                                                           >  2:910044/1‑147 (MQ=255)                    aGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAA‑CT‑GGG‑‑GA‑TTTTTCC‑ggg                                                                                                                                       >  2:1087177/1‑151 (MQ=255)                    aGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTTC‑ggg                                                                                                                                       >  1:846400/1‑151 (MQ=255)                    aGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTAC‑ggg                                                                                                                                       >  2:134349/1‑151 (MQ=255)                      aCCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑ttttttccgg                                                                                                                                        >  1:618985/1‑143 (MQ=255)                        cgcgGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGC‑GG‑TTTTTTC‑ggg                                                                                                                                       >  1:1293482/1‑147 (MQ=255)                         gcggcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGTA‑CT‑GGGG‑GG‑TTTTTTC‑ggggg                                                                                                                                     >  1:1271139/1‑146 (MQ=255)                            gcgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AA‑CGGG‑GG‑TTTTTCC‑GGGGG‑GCT‑gg                                                                                                                              >  2:7460/1‑150 (MQ=255)                             cgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑Ca                                                                                                                           <  2:266233/151‑1 (MQ=255)                             cgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑Ca                                                                                                                           <  1:784550/151‑1 (MQ=255)                             cgTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CT‑TGGC‑GG‑TTTTTTC‑ggg                                                                                                                                       >  1:43161/1‑142 (MQ=255)                                tttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATTGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAA‑AG‑GGGG‑GA‑TTTTACg‑ggggtgttggaa                                                                                                                              >  1:456562/1‑140 (MQ=255)                                tttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAAt                                                                                                                         <  2:743671/150‑1 (MQ=255)                                tttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑ATGGGGG‑GG‑TTTTACC‑GGGGG‑TTG‑GG‑Ca                                                                                                                           >  2:687585/1‑149 (MQ=255)                                 ttGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGGTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAAt                                                                                                                         <  1:881602/149‑1 (MQ=255)                                   gCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATggg                                                                                                                      <  1:1095879/150‑1 (MQ=255)                                   gCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGc                                                                                                                     <  1:1200546/151‑1 (MQ=255)                                   gCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CT‑GGGG‑GG‑TTTTTAC‑ggggggttgggcatggg                                                                                                                         >  1:1150177/1‑136 (MQ=255)                                   gCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AC‑GGGG‑GG‑GTTTACC‑CGGGG‑TGT‑GG‑CAATgggg                                                                                                                     >  1:135030/1‑150 (MQ=255)                                   gCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AA‑GGGG‑GG‑TTTTTCC‑CGGGG‑TGT‑ggggatagg                                                                                                                       >  1:688394/1‑143 (MQ=255)                                     cTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CTGGGGG‑GT‑TTTTACC‑GGG‑G‑GCT‑GG‑CCATgggggg                                                                                                                   >  2:424268/1‑151 (MQ=255)                                       tgtgtgCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGt                                                                                                                  <  1:238382/150‑1 (MQ=255)                                        gtgtgCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTg                                                                                                                 <  2:473438/150‑1 (MQ=255)                                             cttgctCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑AT‑GGGG‑GAGTTTTACC‑‑GGTG‑TCT‑CG‑CCACGGGCgggttg                                                                                                               >  2:654928/1‑143 (MQ=255)                                                   ctGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GA‑TTTTACC‑GGGGG‑TCT‑GG‑CCATGGGGGGGGGGGCGGTTTGt                                                                                                      >  2:1032504/1‑150 (MQ=255)                                                     ggCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGt                                                                                                      <  1:1252457/148‑1 (MQ=255)                                                     ggCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGt                                                                                                      <  1:210757/148‑1 (MQ=255)                                                     ggCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTccc                                                                                                   <  2:667839/151‑1 (MQ=255)                                                     ggCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTccc                                                                                                   <  2:820758/151‑1 (MQ=255)                                                      gCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AA‑GGGG‑GG‑TTTTTTC‑GGGGG‑TGC‑GG‑GGATGGGGGGGGGGGGGgttttttcc                                                                                                   >  1:883941/1‑145 (MQ=255)                                                       ccAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGa                                                                                                 <  1:1105626/151‑1 (MQ=255)                                                            aGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTCC‑CGGGG‑TTT‑GG‑CAAAGGGGGGGGGGGGGGTTTTTccccgcggt                                                                                             >  2:773928/1‑144 (MQ=255)                                                            aGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTAC‑GGGGG‑GCT‑TG‑CCAAGGGGGGGGGGGGGTTTTGTTCCGGCggt                                                                                             >  2:811511/1‑148 (MQ=255)                                                              cGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAA‑CT‑GGGG‑GT‑TTTATCC‑GGGGT‑GCT‑GG‑‑GATGGGGGGGGGGGGGGTTTTTCCCGACCtt                                                                                             >  2:101095/1‑148 (MQ=255)                                                              cGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTACC‑GGGGG‑GGT‑GG‑CCATGGGCGGGGGGGGGGTTTGTCCCGACGGTg                                                                                            >  2:160766/1‑149 (MQ=255)                                                                 cAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CC‑GGGT‑GG‑CTTTTCC‑CGGGG‑TTT‑GGCCAACGGGCGG‑GGGGGGGTTTGTCCCGagggtggtt                                                                                         >  1:898956/1‑141 (MQ=255)                                                                    tAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTTC‑GGGGG‑GCTGGG‑CAAGGGGGGGGGGGGGGTTTTTTCCCGCGGTTGtttt                                                                                        >  2:1039972/1‑147 (MQ=255)                                                                    tAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AC‑GGGG‑GA‑TTTTTCC‑GGGGG‑GCC‑GG‑CCATGGGGGGGGGGGGGGTTTTTTCCGACGTTGGtttttg                                                                                     >  2:307091/1‑146 (MQ=255)                                                                      aCTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGGTGCTGCTGGC‑GGGGAACCG‑GGGG‑GT‑TTTTTCCGGGGTG‑T‑T‑GG‑CATTGGG‑GGGGGGGCGGTTTTTTCCGACGTTGGTTC‑ttgggg                                                                                 >  1:170493/1‑146 (MQ=255)                                                                         gTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGtgat                                                                               <  2:259806/149‑1 (MQ=255)                                                                           tAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTgatgat                                                                            <  2:1252667/150‑1 (MQ=255)                                                                                 cTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTCC‑CGGGG‑GCT‑GG‑CAATGGGGGGGGGGGGGGTTTTTTCCGACGTTGATTC‑T‑GGGGGTGATGCtt                                                                        >  2:1089540/1‑148 (MQ=255)                                                                                   tGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑TGGG‑GG‑TTTTACC‑CGGGG‑TAT‑GG‑TGAATGGAGGTGGGGGGGTTTGTCCCGAAGTTTAttttt                                                                                      >  2:509638/1‑131 (MQ=255)                                                                                                 cGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGttt                                                     <  2:522470/150‑1 (MQ=255)                                                                                                       ggCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑CT‑GGGG‑GG‑TTTTACC‑GGGGG‑TCT‑GG‑CAATGGGCGGGGGGGCGGTTTTTCCCGACGGTGATTT‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTT‑TTgg                                               >  2:817646/1‑149 (MQ=255)                                                                                                         cgcATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGcaca                                           <  2:476165/151‑1 (MQ=255)                                                                                                          gcATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGcacac                                          <  1:822901/151‑1 (MQ=255)                                                                                                            aTGGGCATTTCTGATGATTGTGGCGTGGCTGCTTCTGGC‑GGGGAA‑AG‑AGGC‑GG‑TTTTTAC‑GGGTGTTTT‑GG‑CAATGGGC‑GTGGGGGGGTTTTTTCCGGTGGTGATTT‑TGGGGGTTGGTGCTT‑TCGG‑TCTTGGTGGGGTTG‑TTggggcaaa                                         >  1:293846/1‑144 (MQ=255)                                                                                                             tGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTTC‑GGGGG‑GCT‑GG‑GAATGGGGGGGGGGGGGGTTTTTCCCGACGGTTGTTC‑T‑TGTGGTGATGGGTTTCGA‑TCTTGGGGCGGTTT‑TTTGGACACaa                                        >  1:608059/1‑150 (MQ=255)                                                                                                              gggCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAgg                                      <  1:500869/151‑1 (MQ=255)                                                                                                              gggCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑GGGG‑GG‑TTTTTCC‑GGGGG‑TCT‑GG‑CCATTGGGGGGGGGGGGGTTTTTCCCGGCGTTTGTTT‑T‑GGGGGTGGTGGTTTTCGATTTTTGGGGGGTTTG‑Tgggaaacaa                                         >  1:526329/1‑143 (MQ=255)                                                                                                                gCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCg                                    <  1:222832/151‑1 (MQ=255)                                                                                                                gCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCg                                    <  1:1030772/151‑1 (MQ=255)                                                                                                                 cATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGa                                   <  1:646682/151‑1 (MQ=255)                                                                                                                   tttCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGaa                                  <  1:276022/150‑1 (MQ=255)                                                                                                                     tCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GTGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGaaaa                                <  1:614055/150‑1 (MQ=255)                                                                                                                      cTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑AT‑GGGCGGT‑TTTTACC‑GGG‑G‑GCT‑GG‑CGAAGGGGGGTGGGGCGGTTTTTTCCGAAGGTGGTTCTT‑GGGGATG‑TGACTTTTGC‑GCTTGGGGCGGTTGTTTGGCCCACAAGGGGaaa                                 >  1:571485/1‑149 (MQ=255)                                                                                                                        gatgatTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAaaataa                             <  1:555832/150‑1 (MQ=255)                                                                                                                           gatTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAatat                         <  2:118789/151‑1 (MQ=255)                                                                                                                            atTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAaaataaat                           <  1:32982/148‑1 (MQ=255)                                                                                                                            atTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATg                        <  2:1125496/151‑1 (MQ=255)                                                                                                                               gtgGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTa                     <  1:860971/151‑1 (MQ=255)                                                                                                                               gtgGCGTGGATGCTGCTGGC‑GGGGAA‑AT‑TGGC‑GG‑TTTTTAC‑GGGGG‑GCT‑TG‑CAATGGGGGGGGGGGCGGTTTTTCCCCCCGTTGATTT‑T‑TGGGATGATGCTTATCGA‑TCTTTGGGGGGTTT‑TTTGCACACAAAGGGaaaaaaaa                            >  2:509352/1‑144 (MQ=255)                                                                                                                                 ggCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTaa                    <  1:1211795/150‑1 (MQ=255)                                                                                                                                 ggCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTaa                    <  1:918282/150‑1 (MQ=255)                                                                                                                                 ggCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGGG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTaa                    >  1:1108850/1‑150 (MQ=255)                                                                                                                                    gTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGa                 <  1:98905/150‑1 (MQ=255)                                                                                                                                      ggATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATa               <  2:897287/150‑1 (MQ=255)                                                                                                                                      ggATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAAGATGGTAAAGATAc              <  2:1263937/151‑1 (MQ=255)                                                                                                                                             gctgGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGAt       <  2:846524/151‑1 (MQ=255)                                                                                                                                               tgGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGAtg      >  1:1111569/1‑150 (MQ=255)                                                                                                                                                 gC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGa   <  2:170511/151‑1 (MQ=255)                                                                                                                                                  c‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGaa  <  1:134336/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                               AAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAGCGCTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAA‑CT‑GGGC‑GA‑TTTTACC‑GGGTG‑TCT‑GG‑CAATGGGCGGTGGGGCGTTTTGTCCCGACGTTGATTC‑T‑GGTGATGATGCTTATCGA‑TCTTGGTGCGTTTG‑TTGGCACACAAGGCGAAAATAAATATGGTAAAGATACTCAGGATGTGAA  >  NC_000913/4112429‑4112724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑