| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,346,675 | 0 | T | G | 43.3% | 157.7 / 27.8 | 99 | T218P (ACC→CCC) | fumB | anaerobic class I fumarate hydratase (fumarase B) |
| Reads supporting (aligned to +/- strand): ref base T (11/44); new base G (42/0); total (55/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-17 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT > NC_000913/4346527‑4346825 | aaCGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTAc < 1:96454/151‑1 (MQ=255) gCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑Ca < 1:481894/151‑1 (MQ=255) gACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CAttt < 1:1160170/150‑1 (MQ=255) aCCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTtc < 1:660925/151‑1 (MQ=255) tGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGTGCAGGACCGCGGGGACG‑CCtttttt > 1:1247934/1‑146 (MQ=255) tGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCCGCAGTACCGAGGGGACG‑CCtttttt > 2:124206/1‑146 (MQ=255) gttcgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTcga < 2:1177602/150‑1 (MQ=255) cgttcCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTACCGCGGGGACG‑CATTTTTTcgacg > 1:935429/1‑149 (MQ=255) cccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑g < 2:58035/151‑1 (MQ=255) ccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGGTGCCGGACCGAGGGGAAG‑CATTTtcttgcccaggga > 1:151770/1‑139 (MQ=255) ccTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑Gt < 2:559983/151‑1 (MQ=255) ttCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGCGGGTGCCGGACCGGGGGGACG‑CACTTTTTTGGCGAggga > 1:587384/1‑145 (MQ=255) tCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGGA‑Gtttt > 2:914050/1‑151 (MQ=255) tCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGGA‑Ggttt > 1:72434/1‑151 (MQ=255) ccGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑Gtttt < 2:399501/150‑1 (MQ=255) cGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTc < 1:264528/151‑1 (MQ=255) gTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCAGTAACGAGGGGACG‑CATTTTTTCGACGAGGGA‑Gtttttt > 2:1083728/1‑149 (MQ=255) cGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGGGGCAGGACCGAGGGGACG‑GAATTTTTCGATGAGGAA‑Ggttttttgt > 1:705907/1‑147 (MQ=255) ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGtt < 2:163581/151‑1 (MQ=255) ggCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCCGCCCGACCGGGGGGACG‑CCTTTTTTCCACGGGGGA‑Ggtttt > 1:988373/1‑146 (MQ=255) gCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTGTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGA‑GGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTg < 2:1008019/151‑1 (MQ=255) cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGGTGCAGTACCGAGGGGCCG‑CATTTTTTCGGCGAGGAA‑GGTTTTTAGttt > 1:882068/1‑150 (MQ=255) cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTg < 2:255669/151‑1 (MQ=255) cAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTg < 1:161655/151‑1 (MQ=255) gTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGcc < 2:755967/151‑1 (MQ=255) ttCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGGCGCAGGACCGCGGGGACG‑CATTTTTTCGACGAGGGA‑GATTTTTAGttttgc > 1:988644/1‑147 (MQ=255) ttCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CCTTTTTTCGACGAGGAA‑GGTTTTCAGTTTGcc > 2:911539/1‑150 (MQ=255) cATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCgg < 2:1116664/150‑1 (MQ=255) tAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCCGTAACGAGGGGAGGACATTTT‑TCCACGCGGAAGGTTTTTCGGTTTCCCg > 1:707091/1‑145 (MQ=255) aTAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCCAGGGGACG‑CCTTTTTTTTGCGAGGGA‑GGTTTTTAGTTTGCCggggg > 2:951645/1‑144 (MQ=255) aGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGTACCGGGGGGACG‑CATTTTTTCGGCGAGGGA‑GGTTTTTCGTTTTCCggggggca > 1:823021/1‑142 (MQ=255) gcgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑tt < 1:576988/151‑1 (MQ=255) gcgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑tt < 2:430795/151‑1 (MQ=255) cgcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGTGCAGTACCGAGGGGACG‑CATTTTTTCGACGAGGGA‑GGTTTTTAGTTTTCCGGGGGGCAGCA‑GGGC‑ttt > 2:1175740/1‑151 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGGAGGCTGCAGTAACGAGGGGACG‑CATTTTCTCGACGAGGGA‑GTTTTTTAGTTTTGCGGGGGGCAGCA‑ggggctt > 2:417850/1‑146 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTg < 2:371369/151‑1 (MQ=255) gcTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTg < 2:462491/151‑1 (MQ=255) tGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGCAGTACCGAGGGGACG‑CATTTTCTCGACGAGGAA‑GGTTTTTAGTTTGGCGGGGGGCAGCA‑GGGG‑TTTgggt > 2:96335/1‑149 (MQ=255) cTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCGCCGGTGCCGGGGGGACG‑CCTTTTTTCCACGAGGAAGGTTTTTCA‑TTTGCCGGGGGGGAGGA‑GGGCTTTTGGttt > 1:505046/1‑151 (MQ=255) aaCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCt < 2:437465/151‑1 (MQ=255) aCTTGACGGTTTTCAGTTTGGTTTCCGCAGACGTACCGCCAATCTCAAACGCTATATGGTACGGCGGGCAGGCTGGAGTACCGAGGCTACG‑CATTTTCTCGACCAGGAA‑GTTTTTCAGTTTGCCGGGAGTGAGTA‑GGGC‑TTTGGtt < 1:368404/146‑1 (MQ=255) aCTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTg < 1:451850/151‑1 (MQ=255) ttGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CATTTTCTCGACGAGGGA‑GGTTTTTAGTTTGCCGGGGGTCAGCA‑GGGC‑TTTGGTTTCCTgg > 2:301032/1‑150 (MQ=255) ttGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCAGGGGACG‑GATTTTTTTCCCGAGGAA‑GTTTTTTTGTTTGCCGGGGGGCAGCA‑GGGC‑TTTTGttttc > 1:782747/1‑145 (MQ=255) gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCACTCCCAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTa < 2:553441/150‑1 (MQ=255) gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTag < 1:553698/151‑1 (MQ=255) gACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTag < 2:1277379/151‑1 (MQ=255) gggtttCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGGAGCGAGGGCACG‑CATTTTCTTTAAGAGGAA‑GGTTTTCATTTTGCCGGGGGACAGCA‑GGGG‑CTTGGTTTCCTg > 2:1206737/4‑144 (MQ=255) ggTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTAACGAGGGGACG‑CATTTTTTCGACGAGGAA‑GGTTTTTAGTTTGCCGGGAGGCAGCA‑GGGC‑TTTGGTTTTCTGGGagag > 2:16161/1‑150 (MQ=255) gTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGAt < 1:436526/151‑1 (MQ=255) ttttCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCAGCCGCAGGAACGGGGGGACG‑CCATTTTTCGACGGGGGA‑GGGTTTTAGTTTGCCGGGGGGCAGCA‑GGGC‑TTTTGTTTTCTGGTAgaga > 2:802939/1‑149 (MQ=255) ttCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGCCAGGGTCCACTACCGAGGGGACG‑CAATTTTTCGAAGAGGAA‑GTTTTTCAGTTTTGCGGGGGGCAGCC‑AGGG‑TTTGGTTTCCTCGTAGAGATACg > 2:1253515/1‑151 (MQ=255) tCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACg < 1:559981/150‑1 (MQ=255) cAGGTTGGTTTCCGCGGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CATTTTCTCGACGAGGGA‑GTTTTTTCGTTTGCCGGGGGGCAGCC‑GGGC‑GTTGGGTTCCTGGGAGAGATACGtt > 1:354002/1‑151 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGGGGGGACG‑CATTTTCTCGACGAGGGA‑GTTTTTTAGTTTGCCGGGGGGCAGCA‑GGGC‑GTTGGGTTTCTGGTAGAGATACGtt > 2:1031176/1‑151 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGt < 2:121167/150‑1 (MQ=255) cAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCCCGGGGACG‑CATTTTTTCGACGAGGGA‑GGTTTTTAGTTTGCCGGGGGGGAGCA‑GGGC‑TTTTGTTTTCTGGTGGAGATAAGtt > 1:756742/1‑151 (MQ=255) aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGAGGGGACG‑CATTTTTTCGGCGAGGGA‑GTTTTTTAGTTTGCCGGGAGTCAGCA‑GGGG‑TTTGGTTTTCTGGTAGAGATACGgtt > 1:573023/1‑151 (MQ=255) aGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCCGCAGTACCGGGGGGACG‑CATTTTCTCGACGAGGGA‑GTTTTTTAGTTTGCCGGGAGGCAGCA‑GGGG‑CTTGGTTTCCTGGGAGAGATACGttt > 1:546447/1‑151 (MQ=255) ggttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGGCGGAGGAACGAGGGGACG‑CATTTTTTCGACGAGGAA‑GTTTTTCAGTTTGGCGGGGGGCAGCA‑GGGC‑TTTGGTTTCCTGGGAGAGATACGtttt > 2:1242773/1‑151 (MQ=255) gttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGGACCGAGGGGACG‑CATTTTTTCGACGAGGGA‑GGTTTTCAGTTTGCCGGGGGGCAGCA‑GGGC‑TTTTGTTTCCTGGGTGAGATACGtttt > 2:639191/1‑150 (MQ=255) gttggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAAGCCGCTGCAACGGGGGGACG‑GAATTTCCATTCGGGGGA‑GTTTTTTTGATTGCCGGGAGGCAGTAGGGGC‑TTGGGTTTCTTggagtggtaagtt > 2:1090695/1‑137 (MQ=255) tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCCGGACCGGGGGGACG‑CCTTTTTTCGGCGAGGAA‑GGTTTTTAGTTTGCCGGGGGGCAGCA‑GGGG‑TTTGGGTTTCTGGTAGAGAAACGGtttttt > 2:525484/1‑151 (MQ=255) tggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTttgt < 1:426965/150‑1 (MQ=255) ggttTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTttgtt < 1:726054/150‑1 (MQ=255) ttCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CATTTTTTCGACGAGGGA‑GTTTTTTAGTTTTCCGGGGGGCAGCA‑GGGG‑TTTGGGTTCCTGGTAGAGATACGTTttgtttg > 2:1094208/1‑147 (MQ=255) cGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CCTTTTTTCGACGAGGAA‑GGTTTTTAGTTTGCCGGGAGGCAGCA‑GGGC‑TTTGGTTTTCTTGGAGAGATACGTTTTTTTGGCag > 2:75764/1‑149 (MQ=255) gACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGGACCGAGGGGACG‑CCTTTTCTCGACGAGGAA‑GGTTTTCAGTTTGCCGGGAGGCAGCA‑GGGC‑TTTGGTTTTCTGGTAGAGATACGTTTTTTTGGGAGAgccgc > 2:791098/1‑151 (MQ=255) aCGTACCGCCAATCACAAACGCGATATGGTACGGCGGGAAGGGTGCAGGACCGAGGGGACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATAAGTTTTGTTGGGAGAgccgcc > 2:1018865/1‑151 (MQ=255) cGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgcc < 2:746412/150‑1 (MQ=255) tACCGCCAATCACAAACGCGATATGGTACGGCGGGGAAGCTGCAGGACCCAGGGGACG‑CCTTTTCTCTACGAGGAA‑GGTTTTTAGTTTTGCGGGGGGGAGCA‑GGGC‑TTTGGTTTTCTGGGAGAGATACGGTTTGTTGGCCGAgccgccgcc > 1:770330/1‑151 (MQ=255) tACCGCCAATCACAAACGCGATATGGTACGGCGGGCCGGCTGCAGTACCGAGGGGGCG‑CCTTTTCTCGACGAGGAA‑GTTTTTTAGTTTGCCGGGAGGCAGCA‑GGGC‑TTTGGGTTTCTGGTAGAGGTAAGTTTTGTTTGCAGAgccgccgcc > 1:1107344/1‑151 (MQ=255) tACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CATTTTTTCGACGAGGGA‑GTTTTTCAGTTTGCCGGGGGTCAGCA‑GGGC‑TTTTGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccgcc > 2:24398/1‑151 (MQ=255) tACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGAAG‑CATTTTCTCGACGAGGAA‑GGTTTTTAGTTTTCCGGGAGTCAGCA‑GGGG‑TTTGGTTTCCTGGTAGAGATACGTTTTTTTGGCAGAgccgccgcc > 2:508418/1‑151 (MQ=255) ccGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCtt < 2:648704/151‑1 (MQ=255) gCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTc < 2:587483/151‑1 (MQ=255) ccAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcg < 1:278706/151‑1 (MQ=255) cAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgc < 1:104076/151‑1 (MQ=255) aTCACAAACGCGATATGGTACGGCGGGCAGGGGGGAAGACCGAGGGAACG‑CATTTTTTCGAAGAGGAA‑GGTTTTCCGGTTGCCGGGGGTCAGGA‑GGGG‑CTTTGTTTTCTTGTAGAGGTACGTTTTGTTGGCAGGGCCGGCGCCCTTccta > 2:129712/1‑147 (MQ=255) cacaAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTcgcaac < 2:375807/150‑1 (MQ=255) cgATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CCTTTTTTCGACGAGGGA‑GGTTTTTAGTTTTCCGGGGGGCAGCA‑GGGC‑TTTGGGTTTCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCTCAACGCCAAgg > 1:1032855/1‑149 (MQ=255) cgATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGGACG‑CATTTTCTCGACGAGGAA‑GTTTTTTTGTTTGCCGGGGGTCAGCA‑GGGC‑TTTGGGTTCCTGGGAGAGATACGGTTTGTTGGGCGAGCCGCCGCCCTTTGCAACGCAAAgg > 1:199482/1‑149 (MQ=255) ggTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGt < 1:442998/150‑1 (MQ=255) gTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTa > 1:390724/1‑150 (MQ=255) aCGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTc < 2:28346/151‑1 (MQ=255) cggcggGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTc > 2:526194/1‑150 (MQ=255) ggcggGCAGGCTGCAGTACCGCGGGTACG‑CACTTTCTCGACGAGGAA‑GTTTTTAAGTTTGCCGGGAGCCAGCC‑GGGC‑TTTGGTTTCCTGGTAGAGATTCGTTTTGTCGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGCATTTGTACTc > 1:938216/1‑149 (MQ=255) ggcggGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCAt > 1:21145/1‑151 (MQ=255) gggCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTTAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGc > 1:1103756/1‑151 (MQ=255) gggCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCg < 1:1087203/150‑1 (MQ=255) gCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCa < 2:78012/151‑1 (MQ=255) aGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATc > 2:1053840/1‑151 (MQ=255) gCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTAc < 2:1070268/149‑1 (MQ=255) gCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcg < 2:905416/151‑1 (MQ=255) cAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACcgc > 2:513233/1‑151 (MQ=255) tACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGt > 2:936035/1‑150 (MQ=255) gAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg < 1:1214085/151‑1 (MQ=255) gAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAgg > 1:336735/1‑151 (MQ=255) gTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGa < 2:768380/151‑1 (MQ=255) tACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAt > 2:1041574/1‑151 (MQ=255) | AACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTCCGCAGACGTACCGCCAATCACAAACGCGATATGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACG‑CATTTTCTCGACGAGGAA‑GTTTTTCAGTTTGCCGGGAGTCAGCA‑GGGC‑TTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCTTTCGCAACGCAAAGGAATTTGTACTCATCGCCATCTACCGCGTACAGGTCGAT > NC_000913/4346527‑4346825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |