Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	45.4%	125.1 / 24.5	86	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (10/37);  new base G (39/0);  total (49/37)
Fisher's exact test for biased strand distribution p-value = 1.82e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435                                                                                                                                                     |                                                                                                                                                     ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGa                                                                                                                                                    <  2:164810/150‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGa                                                                                                                                                    <  1:517502/150‑1 (MQ=255)         cGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGATgcg                                                                                                                                            >  1:923915/1‑150 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATgcg                                                                                                                                            <  1:581387/149‑1 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCt                                                                                                                                          <  1:9567/151‑1 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGAGGCGCTgg                                                                                                                                        >  1:379863/1‑150 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGc                                                                                                                                  <  2:830968/151‑1 (MQ=255)                  gACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑CGGGTGGGGGGGAAGATGCGGCGAAAggg                                                                                                                                   >  2:1169798/1‑150 (MQ=255)                     ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTTGGGGGGaaaaaggggtggaaag                                                                                                                                     >  1:1106229/1‑133 (MQ=255)                      gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggt                                                                                                                              <  2:495819/151‑1 (MQ=255)                       ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAAAAGCGGTGAAAagggggggg                                                                                                                             >  1:753019/1‑145 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                             <  1:897156/150‑1 (MQ=255)                        tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGGTGGGGGGGAAGATGCGCCGAAAGGGCCggggg                                                                                                                            >  1:1142737/1‑151 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtgg                                                                                                                            <  1:1128149/150‑1 (MQ=255)                         aTCACTTTATTCTCGGGCGCACGGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGTCAggtgg                                                                                                                            <  2:974569/150‑1 (MQ=255)                             cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGTGAAAGATGCGCTGAAAAGGCAgggggcggtg                                                                                                                       >  2:879583/1‑151 (MQ=255)                             cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGAAGGGCTGGAAGGGGAgggggggggg                                                                                                                       >  1:505705/1‑149 (MQ=255)                             cTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGGTGGGGGGGAAGATGCGCTGGAAGGGCAgggggcgggg                                                                                                                       >  2:588149/1‑149 (MQ=255)                              tttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGCGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGTTGGGGGGAAAGGGGCGGGGAAAGAGCAggtggcggggg                                                                                                                      >  1:539955/1‑151 (MQ=255)                               ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtg                                                                                                                       <  2:1097066/149‑1 (MQ=255)                                tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtggcggtggc                                                                                                                     <  2:1231098/150‑1 (MQ=255)                                     tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑Gtt                                                                                                                 <  2:390528/148‑1 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTg                                                                                                               <  2:142033/149‑1 (MQ=255)                                         gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCAtt                                                                                                          <  1:944698/151‑1 (MQ=255)                                            cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGGTGGGGGGAAAGAAGCGCTGAAAGGGGAGGGGGCGGGGGG‑GGTTGTCAtt                                                                                                          >  1:939661/1‑148 (MQ=255)                                                gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAag                                                                                                   <  1:94526/151‑1 (MQ=255)                                                 aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAaga                                                                                                  <  1:401896/151‑1 (MQ=255)                                                  cGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGAAGCGCTGGAAGGGGCGGGGGCGGGGGC‑GGTTGTCATTTCGaaa                                                                                                    >  1:309306/1‑148 (MQ=255)                                                   gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGGGGTGAAAGGGCAGGGGGCGGGGGC‑GGTTGTCATTCCGaaa                                                                                                    >  2:472340/1‑147 (MQ=255)                                                       tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGATGGGCTGGAAAGGGAGGTGGGGGGGGG‑GGTTGTCCTTCCGGAagagag                                                                                               >  1:827494/1‑148 (MQ=255)                                                       tAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGATGGGCTGAAAGGGGAGGGGGCGGGGGC‑GGTTGTCCTTTCGGAaaag                                                                                                 >  1:907274/1‑146 (MQ=255)                                                        aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGC‑GTTTGTCATTCCGAAAGAGAGCg                                                                                             >  2:159748/1‑149 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGGGGGGGGGAAGAGGCGCTGAAAGGGGAGGGGGCGGGGGC‑GTTTGTCCTTTCGGAagaaag                                                                                               >  1:677829/1‑146 (MQ=255)                                                            aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTc                                                                                       <  2:235171/151‑1 (MQ=255)                                                              cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGTGGCGGGGGC‑GGTTGTCATTCCGAAAGAGAGCGACAGTc                                                                                       >  1:281531/1‑149 (MQ=255)                                                              cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGGTGGGGGGAAAGATGCGCTGGAAGGGGAGGGGGCGGTGGG‑GTTTGTCATTCCGAAAGAGAGCg                                                                                             >  1:1247652/1‑143 (MQ=255)                                                               gTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGGGGGCGGGGGC‑GGTTGTCATTCCGAAAGAGAGCGACAGGCTgg                                                                                    >  1:1199904/1‑151 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGCAAGAGAGCGACAGTCTgg                                                                                    >  2:207510/1‑149 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGTTGGGGGGACAGATTCGCTGAAAAGGCAGGTGCCGGTGGG‑GTGTGATTTTCCCGAAGAGAGAGAAAGGCTTGa                                                                                   >  1:364269/1‑150 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                                  <  1:449753/150‑1 (MQ=255)                                                                     gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTgg                                                                                    >  2:254935/1‑145 (MQ=255)                                                                        cATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTg                                                                           <  2:967082/151‑1 (MQ=255)                                                                           cGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGGGACAGTCTGGGAAAACCTGAtg                                                                        >  1:501431/1‑151 (MQ=255)                                                                            ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGAt                                                                         >  2:1093272/1‑149 (MQ=255)                                                                             gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgtg                                                                      <  1:755155/151‑1 (MQ=255)                                                                             gCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                       >  1:524561/1‑150 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                     <  1:443325/149‑1 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                   <  2:780654/151‑1 (MQ=255)                                                                                  ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGATGCGGTGGAAGGGCAGGTGGCGGTGGC‑GGTTGTCCTTACGAAAGAGAACGACAGTCTGGAAGATCGTGATGGGgggc                                                                  >  2:429040/1‑150 (MQ=255)                                                                                      gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                             <  2:777588/151‑1 (MQ=255)                                                                                         cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAAAGGCGATGGAAAGGGAGGGGGCGGGGGG‑GTTTGTCATTCAGGAAAAGGGAGAAAGTCTGGAAGATCGTGATTTGGGGCACTc                                                              >  1:500551/1‑147 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCCGGTGGGGGGGGG‑GGTTGTCCTTCCCAAAGAGAGCGAAAGTCTGGAAGATCGGGGAGGGGCGCCCTCGc                                                            >  1:1197171/1‑148 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGG‑GGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGTGATGTGGCGCACTCGc                                                            >  1:1257680/1‑148 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGGGGGGGC‑GGTTGGCCTTCCGAAAGAGAGCGGCAGGTTGGAAGAACGTGTTGTGGCGGCCTCGcca                                                          >  1:906549/1‑146 (MQ=255)                                                                                               tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAgaga                                                      >  2:1160222/1‑149 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                                  <  2:64850/151‑1 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                                 <  2:331447/151‑1 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGGAAGAAGCGCTGGAAGGGGCCGGGGCGGCGGG‑GTTTGTCATTCCGGAAGAGAGCGGCAGTCTGGGAGATCGGGGTGTGGGGCCCTCGCCAAAGAAGGCg                                                 >  1:688612/1‑150 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑GGGGTTGGGTGAAAGATGCCCCGAAAGGGCCGGGGGCGGGGGC‑GTTTTTCATTCCCAAAGAGAGCGGAAGTCTGGAAGATCGTGCTGGGGCGCCCTCGCAagagca                                                     >  1:1252820/1‑144 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                           <  2:1255457/150‑1 (MQ=255)                                                                                                           ctcCAGCCATCCGGGCGTTGCCGTAGTGGCGG‑AGGGGGGGGGGAAAGATTTGCTTGAAGGGGAGGGGGCGGGGGG‑GTTTGTTTTTTCGGAAGAGAGCGACAGCCTGGAAGGACGGGAAGTTGCGCAGCCGCAAAAGCAGGCGCTTATgtc                                         >  2:20795/1‑148 (MQ=255)                                                                                                            tcCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑CGGTTGGGGTGAAAGATTCGCTGTAAGGGGGAGTGGCGGGGGCGGTTGGTCTTTCCG‑AAGAGAGCGACCATCCGGAATATCTTTATTTGGCGGACACGCGAGATGAGTGGATTAttggtc                                       >  2:54068/1‑146 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGCCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgcgc                                       <  1:114362/150‑1 (MQ=255)                                                                                                               aGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGGAAGATGCGCTGAAAGGGCAGGGGGGGGTGGG‑GGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                    >  2:942349/1‑151 (MQ=255)                                                                                                                  cATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGGTGGCGGGGGG‑GGTTGTCATTCCGAAAGAGAGCGGCAGTCTTGAAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCtggtgg                                 >  2:1108431/1‑151 (MQ=255)                                                                                                                      cGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAg                             <  2:103770/151‑1 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                            <  2:1175124/151‑1 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGGTGCGCTGAAAAGGGGGGTGGCGGGGGG‑GGTTGGTAATTCGAAAGAGAGCGAACGTCTGGGAGATCGTGGTGTGGCGCACTCGCAAGAGAAGGCGATTTTGGCGCTGGGGGaga                              >  2:1186621/1‑147 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GGTTGTCATTTCGAAAGAGAGCGACAGGCTGGAAGATCGGGATGTGGCGCACTCGCAAGAGAAGGGGGTTATGGCGCTGGGGGGCAg                             >  1:293810/1‑150 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGGGATTATGGCGCTGGTGGACAGc                            >  2:221148/1‑151 (MQ=255)                                                                                                                          cGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAg                         <  2:870012/151‑1 (MQ=255)                                                                                                                           gTTGCCGAAGTGGCGG‑TGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGTGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACAGcc                           >  2:235875/1‑148 (MQ=255)                                                                                                                             tGCCGAAGTGGCGG‑TGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                      >  2:669083/1‑151 (MQ=255)                                                                                                                              gCCGGAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAATATGGCGCTGGTGGACAGCCAGAtt                      <  1:337658/150‑1 (MQ=255)                                                                                                                              gCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                      <  2:557091/150‑1 (MQ=255)                                                                                                                                 gAAGTGGCGG‑TGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGGGGG‑GGTTGTCATTTCGAAAGAGAGCGACAGTCTGGGAGATCGGGATGTGGGGCACTCGCAAGAGAAGGCGATTATGGGGCTGGTGGGCAGCCAGAt                       >  1:434497/1‑146 (MQ=255)                                                                                                                                     tGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt              >  2:284362/1‑151 (MQ=255)                                                                                                                                          g‑tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc         <  2:418106/151‑1 (MQ=255)                                                                                                                                          g‑tggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc         <  1:452799/151‑1 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc       <  1:792991/151‑1 (MQ=255)                                                                                                                                             ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgc       >  2:399435/1‑151 (MQ=255)                                                                                                                                                tGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCg    >  2:551124/1‑151 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg   >  2:1185328/1‑151 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg   <  1:470056/151‑1 (MQ=255)                                                                                                                                                 ggggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg   >  1:364869/1‑151 (MQ=255)                                                                                                                                                  gggTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGgc  <  2:790572/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGG‑TGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGGC  >  NC_000913/354141‑354435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑