Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,637	G→C	E314Q (GAA→CAA)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,637	0	G	C	100.0%	251.5 / NA	61	E314Q (GAA→CAA)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (33/28);  total (33/28)

AAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGG  >  NC_000913/1756491‑1756779                                                                                                                                                   |                                                                                                                                               aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAAt                                                                                                                                             <  1:944838/150‑1 (MQ=255)  aaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATcc                                                                                                                                           >  2:832033/1‑151 (MQ=255)       gTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCaa                                                                                                                                       <  1:705873/150‑1 (MQ=255)          tCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAgg                                                                                                                                   >  2:334071/1‑151 (MQ=255)             gTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTa                                                                                                                                 >  1:1137716/1‑150 (MQ=255)             gTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTa                                                                                                                                 >  2:1156444/1‑150 (MQ=255)                 aCGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAGCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTaaa                                                                                                                               <  1:1149736/148‑1 (MQ=255)                     aaaGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCg                                                                                                                         <  1:137764/150‑1 (MQ=255)                         tCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTgg                                                                                                                     >  2:925135/1‑150 (MQ=255)                                 aCTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGttt                                                                                                            >  2:535342/1‑151 (MQ=255)                                   tGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCt                                                                                                          >  1:879346/1‑151 (MQ=255)                                      gACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATc                                                                                                       >  1:569017/1‑151 (MQ=255)                                      gACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATc                                                                                                       >  1:899370/1‑151 (MQ=255)                                        cccAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCa                                                                                                      <  1:881305/150‑1 (MQ=255)                                           aGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGc                                                                                                  >  2:149037/1‑151 (MQ=255)                                                  ggATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCAt                                                                                            <  1:627123/150‑1 (MQ=255)                                                   gATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCt                                                                                          <  2:189208/151‑1 (MQ=255)                                                    aTTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTg                                                                                         >  2:733558/1‑151 (MQ=255)                                                        cataaccAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGGCAATCCGCAAAAGGTAAATGCCCGCTGGAAGCGGTTTCTATCATGGCGACCATCt                                                                                          <  1:119578/140‑1 (MQ=255)                                                        cATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGa                                                                                      >  2:285325/1‑150 (MQ=255)                                                         aTGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAAc                                                                                    >  2:723574/1‑151 (MQ=255)                                                                     ccACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACcgcg                                                                        >  2:456295/1‑151 (MQ=255)                                                                      cACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGt                                                                       >  2:452133/1‑151 (MQ=255)                                                                       aCGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtg                                                                      >  2:421658/1‑151 (MQ=255)                                                                          cccGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtgat                                                                    <  2:314997/150‑1 (MQ=255)                                                                           ccGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtgatg                                                                   >  2:1029129/1‑150 (MQ=255)                                                                            cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGtgatga                                                                  >  2:690426/1‑150 (MQ=255)                                                                            cGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGaa                                                                 <  1:325383/151‑1 (MQ=255)                                                                             gACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAAc                                                                >  2:57737/1‑151 (MQ=255)                                                                             gACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAAc                                                                <  2:1117432/151‑1 (MQ=255)                                                                                 cgcgCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGcc                                                            >  2:819899/1‑151 (MQ=255)                                                                                 cgcgCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGcc                                                            <  2:487410/151‑1 (MQ=255)                                                                                 cgcgCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGc                                                             <  2:776642/150‑1 (MQ=255)                                                                                   cgcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGt                                                          >  1:241582/1‑151 (MQ=255)                                                                                   cgcAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTCTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGt                                                          >  2:832890/1‑151 (MQ=255)                                                                                    gcACAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtc                                                         >  2:1160877/1‑151 (MQ=255)                                                                                      aGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGtctc                                                       <  2:644993/151‑1 (MQ=255)                                                                                         aGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAg                                                    <  1:53399/151‑1 (MQ=255)                                                                                          gCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGt                                                   <  2:1137909/151‑1 (MQ=255)                                                                                              gTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaa                                               <  2:163054/151‑1 (MQ=255)                                                                                               tGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTcaa                                               >  1:138435/1‑150 (MQ=255)                                                                                                  cGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTAGCGACCGCGTGATGAACAGCCGTCTCGAGTTcaaca                                             <  1:685878/149‑1 (MQ=255)                                                                                                     tGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGa                                         >  1:32677/1‑150 (MQ=255)                                                                                                      gCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGAc                                        >  2:1144647/1‑150 (MQ=255)                                                                                                        aaaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAc                                     >  2:23031/1‑151 (MQ=255)                                                                                                         aaCGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAAcc                                    >  2:1155903/1‑151 (MQ=255)                                                                                                            gCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGt                                  >  2:1282906/1‑150 (MQ=255)                                                                                                                 ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACt                             <  2:646020/150‑1 (MQ=255)                                                                                                                 ccTCGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTg                            <  1:768749/151‑1 (MQ=255)                                                                                                                    cGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGTCCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgcgc                         <  1:849690/151‑1 (MQ=255)                                                                                                                    cGACGGTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTgc                           <  2:553391/149‑1 (MQ=255)                                                                                                                        ggTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTa                     <  1:258273/151‑1 (MQ=255)                                                                                                                        ggTACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTa                     <  2:1007903/151‑1 (MQ=255)                                                                                                                          tACTGACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTAcc                   >  2:1143612/1‑151 (MQ=255)                                                                                                                              gACGCAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGa                 >  2:86394/1‑149 (MQ=255)                                                                                                                                  cAGTGATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGt           <  2:613901/151‑1 (MQ=255)                                                                                                                                      gATGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGc       >  2:933180/1‑151 (MQ=255)                                                                                                                                       aTGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGcc      <  1:1223421/151‑1 (MQ=255)                                                                                                                                       aTGCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGcc      <  2:1168302/151‑1 (MQ=255)                                                                                                                                         gCTGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgt    <  1:543714/151‑1 (MQ=255)                                                                                                                                           tGTCTGGTCAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCgtgg  >  1:292825/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                               AAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATGGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGG  >  NC_000913/1756491‑1756779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑