Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,969,313 C→A E18* (GAG→TAG)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,969,3130CA97.1% 268.4 / 0.4 70E18* (GAG→TAG) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (34/34);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

CGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCG  >  NC_000913/1969166‑1969459
                                                                                                                                                   |                                                                                                                                                  
cGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGt                                                                                                                                                 <  2:1139542/151‑1 (MQ=255)
              cccAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAAt                                                                                                                                    >  1:1005848/1‑150 (MQ=255)
              cccAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAAt                                                                                                                                    <  1:331777/150‑1 (MQ=255)
                  gCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGa                                                                                                                               <  1:278173/151‑1 (MQ=255)
                   cGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGAc                                                                                                                              <  1:960476/151‑1 (MQ=255)
                    gAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGAc                                                                                                                              <  2:849522/150‑1 (MQ=255)
                     aaCGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGt                                                                                                                            >  2:612037/1‑151 (MQ=255)
                         cAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGc                                                                                                                        >  2:208786/1‑151 (MQ=255)
                             cgacgaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCcac                                                                                                                    <  2:203605/151‑1 (MQ=255)
                             cgacgaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCca                                                                                                                     <  1:946962/150‑1 (MQ=255)
                               acgaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAg                                                                                                                  <  2:110876/151‑1 (MQ=255)
                               acgaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAg                                                                                                                  <  2:1130598/151‑1 (MQ=255)
                                cgaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAgg                                                                                                                 >  1:736103/1‑151 (MQ=255)
                                 gaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAggg                                                                                                                >  2:1260234/1‑151 (MQ=255)
                                  aaCCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGc                                                                                                               <  1:769845/151‑1 (MQ=255)
                                    ccAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGc                                                                                                               >  1:551732/1‑149 (MQ=255)
                                         cGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGAt                                                                                                         <  1:1002765/150‑1 (MQ=255)
                                           gTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGaa                                                                                                      <  2:289132/151‑1 (MQ=255)
                                           gTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGa                                                                                                       >  2:79593/1‑150 (MQ=255)
                                                aaaCCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCa                                                                                                  <  2:182539/150‑1 (MQ=255)
                                                aaaCCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCAt                                                                                                 >  2:463319/1‑151 (MQ=255)
                                                    cATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAgcg                                                                                             <  2:619088/151‑1 (MQ=255)
                                                    cATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAgcg                                                                                             >  2:671221/1‑151 (MQ=255)
                                                      tGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGcc                                                                                           >  1:71001/1‑151 (MQ=255)
                                                         cgcgTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCtt                                                                                         <  1:536620/150‑1 (MQ=255)
                                                         cgcgTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCtt                                                                                         <  2:928370/150‑1 (MQ=255)
                                                          gcgTTTATGGTTAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTtct                                                                                       <  1:1153209/151‑1 (MQ=255)
                                                           cgTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTtctc                                                                                      >  1:3976/1‑151 (MQ=255)
                                                            gTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCa                                                                                     <  2:16302/151‑1 (MQ=255)
                                                              ttATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAAt                                                                                   >  2:233125/1‑151 (MQ=255)
                                                                   gTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACtt                                                                              <  1:1004626/151‑1 (MQ=255)
                                                                      aGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTc                                                                             >  1:913898/1‑149 (MQ=255)
                                                                         cAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGAt                                                                        >  2:726986/1‑151 (MQ=255)
                                                                         cAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGAt                                                                        >  2:1156322/1‑151 (MQ=255)
                                                                          aGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGAt                                                                        >  1:975068/1‑150 (MQ=255)
                                                                             aCGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATAcc                                                                     >  1:877364/1‑150 (MQ=255)
                                                                               gATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACt                                                                  >  1:146529/1‑151 (MQ=255)
                                                                                   ccGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGgcg                                                              >  1:497715/1‑151 (MQ=255)
                                                                                       cTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAAt                                                          >  2:608200/1‑151 (MQ=255)
                                                                                         cGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAAt                                                          <  1:111673/149‑1 (MQ=255)
                                                                                         cGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAAt                                                          <  2:272175/149‑1 (MQ=255)
                                                                                            tGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGt                                                      >  2:960172/1‑150 (MQ=255)
                                                                                            tGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGt                                                      <  1:384808/150‑1 (MQ=255)
                                                                                             gATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTa                                                     <  2:681163/150‑1 (MQ=255)
                                                                                              aTAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAAc                                                   >  2:1185281/1‑151 (MQ=255)
                                                                                                aGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTg                                                 <  2:73050/151‑1 (MQ=255)
                                                                                                   tCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAc                                              >  2:107397/1‑151 (MQ=255)
                                                                                                    cAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAcc                                             >  2:770284/1‑151 (MQ=255)
                                                                                                    cAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCAc                                              <  2:1132471/150‑1 (MQ=255)
                                                                                                       ttGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGAc                                          >  2:104688/1‑151 (MQ=255)
                                                                                                        tGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGAc                                          <  1:933501/150‑1 (MQ=255)
                                                                                                         gACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTc                                        >  2:450598/1‑151 (MQ=255)
                                                                                                          aCTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTc                                        >  1:1083568/1‑150 (MQ=255)
                                                                                                          aCTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTc                                        >  2:856208/1‑150 (MQ=255)
                                                                                                                 cgccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTg                                >  1:576180/1‑151 (MQ=255)
                                                                                                                 cgccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTg                                <  1:666145/151‑1 (MQ=255)
                                                                                                                   ccgAAAATGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCt                              <  2:1104026/151‑1 (MQ=255)
                                                                                                                       aaaTGCGCGTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACtt                          <  1:291043/151‑1 (MQ=255)
                                                                                                                              cgTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttc                   >  2:822886/1‑151 (MQ=255)
                                                                                                                               gTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttct                  <  1:953713/151‑1 (MQ=255)
                                                                                                                               gTCGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttct                  <  1:875514/151‑1 (MQ=255)
                                                                                                                                 cGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                >  1:191402/1‑151 (MQ=255)
                                                                                                                                 cGGAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttctt                 <  2:305864/150‑1 (MQ=255)
                                                                                                                                  ggAAAGCGCCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGttcttc                >  2:195339/1‑150 (MQ=255)
                                                                                                                                        cgcCAGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTa         <  2:252115/151‑1 (MQ=255)
                                                                                                                                            aGGCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATa     <  1:272772/151‑1 (MQ=255)
                                                                                                                                             ggCGCTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATAc    <  2:778717/151‑1 (MQ=255)
                                                                                                                                              gcgcTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATAcc   >  2:577226/1‑151 (MQ=255)
                                                                                                                                               cgcTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCg  >  1:1166868/1‑151 (MQ=255)
                                                                                                                                               cgcTAGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCg  >  2:904738/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCG  >  NC_000913/1969166‑1969459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: