Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,859,6600CA43.2% 69.5 / 18.3 44L190F (TTG→TTT) ydjKputative MFS sugar transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base C (22/3);  new base A (0/19);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 2.19e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGCAAAGGTTTACCCGTCTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGACCACGTTCCACGATTTCTAC  >  NC_000913/1859514‑1859805
                                                                                                                                                   |                                                                                                                                                 
ggcggcAAAGGTTTACCCGTCTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAata                                                                                                                                               <  2:88872/151‑1 (MQ=255)
              aCCCGTCTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGc                                                                                                                                 >  1:219110/1‑151 (MQ=255)
                ccGTCTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAg                                                                                                                               <  1:727812/151‑1 (MQ=255)
                   tCTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATCCCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCATGTATAAGCAGtt                                                                                                                             <  2:217200/150‑1 (MQ=255)
                    cTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGt                                                                                                                           >  2:796834/1‑151 (MQ=255)
                     tGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGt                                                                                                                           >  1:6869/1‑150 (MQ=255)
                        cGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTAccc                                                                                                                       >  2:433590/1‑151 (MQ=255)
                                  cttcttCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTcc                                                                                                              >  2:559468/1‑150 (MQ=255)
                                       tctATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCActct                                                                                                         >  2:1076550/1‑150 (MQ=255)
                                        ctATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGc                                                                                                       >  2:885902/1‑151 (MQ=255)
                                                cgaatgttttccgcGGCTTCCTGATCCCGCCCGCGCGATCCCAGCCGGGGGGGGGATT‑AAGAAAAGTAGCGCCGGGCCGGCGCCGTGGAAAAAAGCAAAAATATGGCGGGGATAAGCGGTTGTCCCCGCCAGTTCCACTCTGCACTAATCa                                                                                               <  2:860514/138‑1 (MQ=255)
                                                   aTGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAgcg                                                                                            >  1:918856/1‑151 (MQ=255)
                                                           ctcGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGt                                                                                    >  1:375463/1‑151 (MQ=255)
                                                           cgcGGCTTCCTGATCCCGTCCGCGCGATCCCACCCAGCGGGGGGTTT‑CAGAAAAGTGGCCCCGGCCGAGCGCCGTGGCAAAAAGCGAAATTATGGCAGGGAAAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAg                                                                                     <  1:539716/148‑1 (MQ=255)
                                                            tcGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTc                                                                                   >  1:995988/1‑151 (MQ=255)
                                                                cTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCAt                                                                               >  2:44081/1‑151 (MQ=255)
                                                                 ttCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATc                                                                              >  2:919194/1‑151 (MQ=255)
                                                                   cttgaccccgcccgcGCAATCCCACCCGGGGGGGGGATT‑AAGAAAGGGGGCCCCGGGCAGCCGCGTTGGCAATAAGCGAAAATTTGGCAGGAAAAAGCAGTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTcc                                                                                  <  1:531262/138‑1 (MQ=255)
                                                                   cctaaccccgcccgcGCGATCCAAGCCAGCGGGGGGATT‑AAGAAAAGGAGCGCGGGCCCGGCGCGGTAGCAAAAGGCAAAAATATGGGGGGAAAAAGAATTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATc                                                                              <  1:642085/142‑1 (MQ=255)
                                                                   ccggATCCCGCCCGCGCAATCCCAGCCGGGGGGGGGTTT‑AGGGAAAGTGGCGCCCGCCCGGCGCCGTAGCGATAACCAAAATTATGGCAGGGATAGGTTGTGTTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGt                                                                                    <  2:206178/140‑1 (MQ=255)
                                                                            gTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATg                                                                    >  2:313679/1‑150 (MQ=255)
                                                                              ccGCGCGTTCCCAGCCGGCGGGGGGATT‑AAGGAAAGTAGCGCCAGGCCAGCGCCGGAGCAAAAAGCAAAAATTTGGAAGGAAAAAGCTTTTGCACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAAc                                                                 <  2:1053629/151‑1 (MQ=255)
                                                                              ccGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAAc                                                                 >  2:1122745/1‑151 (MQ=255)
                                                                              ccGCGCGATCCCAGCCGGGGGGGGGTTA‑AAGAAAAGTGGCGCCGGGCCGGCGCCGAAGCAATAAGCAAAAATATGGAGGGAATAAGTTGTTGCCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGa                                                                   <  2:244629/149‑1 (MQ=255)
                                                                                                 ggggggATA‑CAGAAAGGAAGCGCCGGCCCGGCGCCGTAGCGAAAAGCAAAAATTTTGGGGGAAAAGGGTGTTGCCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGtt                                              <  2:839797/149‑1 (MQ=255)
                                                                                                  gggggATTGCAGGAAGGGAGCCCAAGGCGAGCGCGGAAGGAAAAACGGAAAATTTAGCAGGAGTAAACGTTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGt                                               <  1:940932/149‑1 (MQ=255)
                                                                                                  cgGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTg                                             >  1:500921/1‑151 (MQ=255)
                                                                                                             gaaaaGTAGCGCCAGGCCAGCGCCGTAGCAAAAAGCAAAAATATGGCGGGGATAAGCATTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATaa                                      <  1:891132/146‑1 (MQ=255)
                                                                                                               aaggtggcgcCAGCCCGGCCCGTTAGCGAAAAGCGAAAATATTGAGGGGATAAGCTGTGTCCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATa                                       <  2:756482/139‑1 (MQ=255)
                                                                                                               aagggggcgcCGGGCCAGCGCGGTAGAAAAAAGCGAAAATATGGGGGGAAAAAGCGGTTTTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAgg                                   <  2:513891/143‑1 (MQ=255)
                                                                                                               aaatttgcgcCAGCCCGGCGCCGTAGCGATAGGCGAAAATATGGCAGGGATAAGCGTTTTTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGa                                  <  1:731369/144‑1 (MQ=255)
                                                                                                               aaaGGACCGCCAGCCAGGCGCCGAAGCAATAACCAAAAATATTGCGGGAATAGGCTGTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAAt                                        <  1:46259/144‑1 (MQ=255)
                                                                                                                   tAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAAccc                             >  1:864064/1‑151 (MQ=255)
                                                                                                                    aGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAAccc                             >  2:283418/1‑150 (MQ=255)
                                                                                                                      cgcCAGCCCGGCGCCGGAGGAAAAAGCAAAAATATGGAGGGAAAAAGCTGTTTTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAAccc                             <  2:409113/148‑1 (MQ=255)
                                                                                                                        ccggcccagCGCCGTGGAGAAAAGCAAAAATATGGCAGGAATAAGCAGTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACtt                        <  1:1154716/146‑1 (MQ=255)
                                                                                                                          aggccagCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTg                       >  2:302654/1‑150 (MQ=255)
                                                                                                                           ggccagCGCGGTAGCAAAAAGCAAAAATATGGCGGGAATAAGCGTTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGAc                          <  1:1114372/146‑1 (MQ=255)
                                                                                                                           ggccagCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGAc                     >  2:913373/1‑151 (MQ=255)
                                                                                                                              cggcgcGGTAGAGAAAAGCGAAAATATGGCGGGAATAAGCGGTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGAcc                    <  1:349079/147‑1 (MQ=255)
                                                                                                                                  gcCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGACCACGTTc              >  1:1111767/1‑151 (MQ=255)
                                                                                                                                           aaaggCGAAAATATGGCAGGGAAAAGCTTTTTTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGa                      <  1:382291/130‑1 (MQ=255)
                                                                                                                                             aaGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGACCACGTTCCACGATTTCt    >  2:92478/1‑150 (MQ=255)
                                                                                                                                              aGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGACCACGTTCCACGATTTCTAc  >  2:253964/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCGGCAAAGGTTTACCCGTCTGGCGTATGACGCCTTCTTCTATACTCCGCATGACTTTCTCGGCTTCCTGATACCGTCCGCGCGATTCCAGCCAGCGCGGGGATT‑CAGGAAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAATAAAGGAAACCCGACTTGACCACGTTCCACGATTTCTAC  >  NC_000913/1859514‑1859805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑