Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC41.4% 159.9 / 31.0 87V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (48/3);  new base C (0/36);  total (48/39)
Fisher's exact test for biased strand distribution p-value = 2.50e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443129‑3443423
                                                                                                                                                        |                                                                                                                                                  
aGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcc                                                                                                                                                  >  1:968307/1‑151 (MQ=255)
  aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAcccc                                                                                                                                                >  2:711805/1‑151 (MQ=255)
   cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                                                               <  2:240994/151‑1 (MQ=255)
        agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCat                                                                                                                                          >  2:264886/1‑151 (MQ=255)
          agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCatat                                                                                                                                        >  1:381378/1‑151 (MQ=255)
           gTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCatat                                                                                                                                        <  1:308646/150‑1 (MQ=255)
           gTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATAtt                                                                                                                                       >  1:50699/1‑151 (MQ=255)
           gTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATAtt                                                                                                                                       >  1:70633/1‑151 (MQ=255)
                acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAGTAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                                  >  2:10009/1‑151 (MQ=255)
                acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                                  >  2:402641/1‑151 (MQ=255)
                  aTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                >  1:682032/1‑151 (MQ=255)
                   aaaggCGGTGCCCCAGGCTAAAAATACAGCAAATTTGTTGTCCGCCAGTCCCACCCAGTCCCCCCCCCAAACCAGGCCGCGTGGGTCGCCGGAAACAAAAAAATACTGGAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCa                                                                                                                               <  2:324661/147‑1 (MQ=255)
                      aGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCg                                                                                                                            >  1:621697/1‑151 (MQ=255)
                             ccccGGGCTAAAAATACAGAGAATTTTTTGCCACCCATTCCCAACAAGCCCCCCCCCCAAACCAGGCGGCGGGGGCCGCGGGAAAAAAAATAATAGGGGAAGAAAAAATGGGGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                       <  2:629282/148‑1 (MQ=255)
                              cccagccgcaaaaCACAGCAAATTTGTTGCCCCCCTTTCCCACCCAGTCCCCCCCCCAACCCATGACGCGTTGGCCCCCGGAAAAAAAAAAATACGGGAAGAGAAAATGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                       <  2:689816/138‑1 (MQ=255)
                               ccaggtaacAAATACAGCAAATTTGTTGTCAGCAATTTCAACCAAGTCCCCCCCCCAAACCAGGACGCGAGGGTCGCGGGAAAAAAATTAATACGGGAAGCGAAGATGGCGGGAATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                   <  1:1132552/143‑1 (MQ=255)
                                        aaaaaaagggAATTTTTTGCCAGCCATTCCCACCCAGTCCCGCCCCCAACCCAGGACGCGATGGTCGCGGGGAAAAAAATAATTCTGGAAGTAAAAATTGCGGGGATACCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                  <  2:1139167/134‑1 (MQ=255)
                                              agaaaatttgttgCCAGCCAGTCCCAACCAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGAAACAAAAAAATACTGGAAGCGAAGTTGGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                      <  1:639000/142‑1 (MQ=255)
                                                 gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                    >  1:1219557/1‑148 (MQ=255)
                                                  aattttttgtCAGCCAGTCCCAACCAGTCCCCCCCCCGAACCATGCCGCGATGGTCGCGGGAAACAAAATAATACGGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGa                                                                                                 <  1:1115125/144‑1 (MQ=255)
                                                    attttttgtcgCCCGTTCCAACCCGGCCCCCCCCCCGAACCATGACGCGATGGTCGCGGGGAAAAAAATAATAGGGGAGGCGAAGAGGGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                <  2:70651/140‑1 (MQ=255)
                                                    aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                              >  1:453155/1‑151 (MQ=255)
                                                      ttgttgccgCCCGTTCCAACCCGGTCCCCCCCCCAACCCAGGACGGGTGGGTCGCGGGAAAAAAAAAAATAGTGGAAGGAAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagca                                                                                                     <  2:1116620/135‑1 (MQ=255)
                                                        ttgcCAGCCAGTCCCACCAAGTCCCCCCCCCGAACCATGACGCGTTGGTCGCGGGAAAAAAAATAATCCTGAAAGCGAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                           <  1:300387/150‑1 (MQ=255)
                                                           ccgcccAGTCCCACCCATCCCCCCCCCCGAACCAGGACGGGATGGTCGCGGGAAAAAAAATAATACTGGAAGCGAAGTTTGCGGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACAcgacga                                                                                         <  2:195581/145‑1 (MQ=255)
                                                            cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                      >  1:953475/1‑151 (MQ=255)
                                                             aGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                     >  1:921520/1‑151 (MQ=255)
                                                               ccAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGc                                                                                   >  1:676255/1‑151 (MQ=255)
                                                               ccAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGc                                                                                   >  2:48110/1‑151 (MQ=255)
                                                                 aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 >  1:339714/1‑151 (MQ=255)
                                                                 aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 >  1:1007399/1‑151 (MQ=255)
                                                                 aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACACCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 >  1:374124/1‑151 (MQ=255)
                                                                  attaccaacagtcaCCCCCCCCCACCCATGTCGCGAGGGTCCCGGGAACAAGAAAAATCCGGGAGGCAAATTTGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                  <  2:106535/137‑1 (MQ=255)
                                                                     ccAACCAGCCCCGCCCCAAACGCGTGACGCGATGGCCGCGGGAAACAAAAAAATGCGGGAAGCAAGGATTGCGGGGTTTCCCGCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                    <  1:189968/144‑1 (MQ=255)
                                                                      caagccagccccGCCCCCGCACCGGGGCGCAAGGGGCGCGAGGAGAAGAAAAATAATGGAGGGAAGTTTTGCTGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                               <  1:636251/139‑1 (MQ=255)
                                                                        aCCAGTCCCCCCCCAGAACAATGCCGCATGGGTCGCAGGAAACAGAAAAATATTGGAAGGGAAGATTGCCGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                          <  2:978050/151‑1 (MQ=255)
                                                                          ccagccccGCCCCCAACCCATGCCGGGATGGTCGCGGGAAAAAAAATAATACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                          <  1:310729/144‑1 (MQ=255)
                                                                          ccAGTCCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGAGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                          <  2:1076250/150‑1 (MQ=255)
                                                                          ccAGTCCCCCCCCAGACCCATGAGGGGATGGTCGCGGGAAAAAAAATAAAACTGGAAGCGAAAATTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCTGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                          <  2:639731/150‑1 (MQ=255)
                                                                           cAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                        >  1:715737/1‑151 (MQ=255)
                                                                              tACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                      >  1:876779/1‑150 (MQ=255)
                                                                                cccccccccAAACCATGGCGGGAGGGTCCCCGGAAACAAAAAAATACTGGAAGAAAAGTTGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                     <  2:936377/146‑1 (MQ=255)
                                                                                       ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                              >  2:113097/1‑150 (MQ=255)
                                                                                          aaCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                           >  2:848027/1‑150 (MQ=255)
                                                                                          aaCCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                           >  1:1088517/1‑150 (MQ=255)
                                                                                                  cgcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                  >  1:1063001/1‑151 (MQ=255)
                                                                                                   gggATGGTCGCGGGAAAAAAAAAAATACTGGAAGGGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                       <  1:109003/143‑1 (MQ=255)
                                                                                                   gcgATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                 >  1:7078/1‑151 (MQ=255)
                                                                                                     gtgggTCGCGGGAAACAAAAAAATACGGAAAGCGAAGTTGGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                                 <  1:1102229/146‑1 (MQ=255)
                                                                                                     gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                >  1:647701/1‑150 (MQ=255)
                                                                                                       tGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                             >  1:410816/1‑151 (MQ=255)
                                                                                                       tGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAcc                                             >  2:106725/1‑151 (MQ=255)
                                                                                                        ggTCGCGGGAAACAAAATAATACGGGAAGCAAAGATTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                            <  1:3540/151‑1 (MQ=255)
                                                                                                             gcgggaaaaaaaaaaataCGGAAAGCGAAGTTTGCCGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                <  2:192921/129‑1 (MQ=255)
                                                                                                               ggaaaCAAAATAATACGGGAAGCGAAGTTTGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                           <  2:333946/142‑1 (MQ=255)
                                                                                                                ggaaaaaaaataatACTGGAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                       <  1:855599/141‑1 (MQ=255)
                                                                                                                    cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  1:787222/1‑151 (MQ=255)
                                                                                                                    cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  1:1151665/1‑151 (MQ=255)
                                                                                                                    cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  1:851935/1‑151 (MQ=255)
                                                                                                                      ataataatACGTGAAGCAAAGATTGCCGGCATGCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                               <  2:47715/149‑1 (MQ=255)
                                                                                                                      aaaataatACTGGAAGCAAAGTTTGCCGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                <  2:403823/148‑1 (MQ=255)
                                                                                                                      aaaaaattccTGGAAGCGAAGATGGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaaca                                   <  2:234500/138‑1 (MQ=255)
                                                                                                                      aGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  2:613931/1‑150 (MQ=255)
                                                                                                                        aataatACGGGAAGCGAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                             <  2:1062983/151‑1 (MQ=255)
                                                                                                                         ataataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                             >  1:497114/1‑150 (MQ=255)
                                                                                                                           atgccggaaaGCGAAGATGGCCAGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                          <  1:653557/143‑1 (MQ=255)
                                                                                                                           aataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                           >  2:31604/1‑150 (MQ=255)
                                                                                                                                 ggAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                    >  1:400689/1‑151 (MQ=255)
                                                                                                                                 ggAAGAGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                     <  1:1057665/150‑1 (MQ=255)
                                                                                                                                 gaaaGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                    <  2:42890/149‑1 (MQ=255)
                                                                                                                                   aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                  >  2:24307/1‑151 (MQ=255)
                                                                                                                                   aaGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                  >  2:371638/1‑151 (MQ=255)
                                                                                                                                   aaGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                  <  1:192599/151‑1 (MQ=255)
                                                                                                                                   aaGAGATGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                     <  2:371549/148‑1 (MQ=255)
                                                                                                                                     gCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                 >  2:1127973/1‑150 (MQ=255)
                                                                                                                                     gCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                 >  2:620793/1‑150 (MQ=255)
                                                                                                                                      cGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc               >  1:523824/1‑151 (MQ=255)
                                                                                                                                      cGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc               >  1:1169251/1‑151 (MQ=255)
                                                                                                                                       gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc               >  2:1148671/1‑150 (MQ=255)
                                                                                                                                        aaGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt              <  1:465475/150‑1 (MQ=255)
                                                                                                                                         aGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc            >  2:819330/1‑151 (MQ=255)
                                                                                                                                         aGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc            >  1:152030/1‑151 (MQ=255)
                                                                                                                                          gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc            >  2:742544/1‑150 (MQ=255)
                                                                                                                                               gCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa          <  2:319238/148‑1 (MQ=255)
                                                                                                                                                  gggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact     >  1:313157/1‑150 (MQ=255)
                                                                                                                                                   ggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaa   <  2:1031240/151‑1 (MQ=255)
                                                                                                                                                     aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat  >  1:687809/1‑150 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
AGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443129‑3443423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: