Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,187,619	C→A	T757K (ACG→AAG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,187,619	0	C	A	100.0%	295.1 / NA	74	T757K (ACG→AAG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/38);  total (36/38)

AACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGA  >  NC_000913/4187471‑4187766                                                                                                                                                     |                                                                                                                                                    aaCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGc                                                                                                                                                   >  1:852677/1‑151 (MQ=255)  aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGcc                                                                                                                                                  <  1:219024/151‑1 (MQ=255)  aCCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGc                                                                                                                                                   >  2:1194618/1‑150 (MQ=255)   ccGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCa                                                                                                                                                 <  2:1021447/151‑1 (MQ=255)   ccGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCa                                                                                                                                                 <  2:575557/151‑1 (MQ=255)    cGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCaa                                                                                                                                                <  1:839111/151‑1 (MQ=255)     gTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAAt                                                                                                                                               <  2:527458/151‑1 (MQ=255)        aCGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCAc                                                                                                                                            <  1:46308/151‑1 (MQ=255)              aGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGaa                                                                                                                                      <  2:403277/151‑1 (MQ=255)              aGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGaa                                                                                                                                      <  1:211250/151‑1 (MQ=255)                gAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACt                                                                                                                                    >  1:537388/1‑151 (MQ=255)                gAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACt                                                                                                                                    <  1:1070400/151‑1 (MQ=255)                       aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGGTGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTg                                                                                                                              >  2:1090645/1‑150 (MQ=255)                       aGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTg                                                                                                                              >  2:26700/1‑150 (MQ=255)                         cAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGa                                                                                                                             >  1:65974/1‑149 (MQ=255)                          aGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAgg                                                                                                                          >  2:1016973/1‑151 (MQ=255)                               tCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTg                                                                                                                      <  1:287577/150‑1 (MQ=255)                               tCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTg                                                                                                                      >  2:556922/1‑150 (MQ=255)                                 cTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc                                                                                                                   >  2:44306/1‑151 (MQ=255)                                   tCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAAc                                                                                                                   <  1:581762/149‑1 (MQ=255)                                    cAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGt                                                                                                                 <  1:740899/150‑1 (MQ=255)                                        aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTcc                                                                                                            >  1:493030/1‑151 (MQ=255)                                        aGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTcc                                                                                                            >  2:518684/1‑151 (MQ=255)                                         gCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCa                                                                                                           <  1:308374/151‑1 (MQ=255)                                          cATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTcc                                                                                                            >  1:419201/1‑149 (MQ=255)                                           aTCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGt                                                                                                         >  2:57991/1‑151 (MQ=255)                                            tCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGt                                                                                                         >  2:605513/1‑150 (MQ=255)                                               aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGt                                                                                                         <  2:721010/147‑1 (MQ=255)                                               aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACtt                                                                                                     <  2:361126/151‑1 (MQ=255)                                               aCATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACtt                                                                                                     <  2:1015848/151‑1 (MQ=255)                                                 atgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTc                                                                                                    >  2:451489/1‑150 (MQ=255)                                                 atgatgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTc                                                                                                    >  2:830477/1‑150 (MQ=255)                                                      ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCAtctc                                                                                               <  1:818544/150‑1 (MQ=255)                                                      ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTcc                                                                                              >  2:988201/1‑151 (MQ=255)                                                        ccGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccac                                                                                            <  2:1060800/151‑1 (MQ=255)                                                         cGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccacc                                                                                           >  2:472883/1‑151 (MQ=255)                                                           aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTccaccca                                                                                         <  1:1016078/151‑1 (MQ=255)                                                             tCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACg                                                                                       >  2:72511/1‑151 (MQ=255)                                                                   gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTc                                                                                 <  1:1059513/151‑1 (MQ=255)                                                                   gcgcGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTc                                                                                 <  2:593095/151‑1 (MQ=255)                                                                     gcgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGt                                                                               <  1:123603/151‑1 (MQ=255)                                                                      cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTa                                                                              <  2:544579/151‑1 (MQ=255)                                                                         ggTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTaa                                                                             >  2:612221/1‑149 (MQ=255)                                                                            tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                         <  2:418500/150‑1 (MQ=255)                                                                            tCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTc                                                                        >  1:1063684/1‑151 (MQ=255)                                                                               gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCt                                                                       <  2:473221/149‑1 (MQ=255)                                                                               gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTg                                                                      <  2:1068461/150‑1 (MQ=255)                                                                                 ggCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCg                                                                   >  2:1217745/1‑151 (MQ=255)                                                                                      aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATa                                                               <  2:591081/150‑1 (MQ=255)                                                                                          tCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGc                                                           >  1:290263/1‑150 (MQ=255)                                                                                            gTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACt                                                        >  2:460551/1‑151 (MQ=255)                                                                                              cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTg                                                       <  1:458337/150‑1 (MQ=255)                                                                                               aGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGa                                                      >  2:707403/1‑150 (MQ=255)                                                                                                gCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                    <  2:895964/151‑1 (MQ=255)                                                                                                    gCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGAAACCGCACTGAAAACt                                                 <  2:514744/150‑1 (MQ=255)                                                                                                       ggTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGc                                               <  1:50370/149‑1 (MQ=255)                                                                                                        gTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGa                                             >  2:474735/1‑150 (MQ=255)                                                                                                          aTGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACt                                          >  2:32235/1‑151 (MQ=255)                                                                                                           tGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                         >  2:609173/1‑151 (MQ=255)                                                                                                                ggTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGtt                                    <  1:353973/151‑1 (MQ=255)                                                                                                                 gTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGtt                                    >  1:577485/1‑150 (MQ=255)                                                                                                                 gTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTa                                   <  2:977242/151‑1 (MQ=255)                                                                                                                     gATGGCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTg                               <  1:819340/151‑1 (MQ=255)                                                                                                                        ggCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACt                            >  2:295357/1‑151 (MQ=255)                                                                                                                         gCGAAGCCGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtc                           >  1:460676/1‑151 (MQ=255)                                                                                                                                cGGATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCt                    >  2:224004/1‑151 (MQ=255)                                                                                                                                 ggATGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTg                   >  1:818227/1‑151 (MQ=255)                                                                                                                                 ggATGGCTCCATCATCGAAAAGCCAAGCGCCGCGAACTTCCGTGAAGGGCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAGAGGTCTGGCGGATGCCGCACTGAAAACTGCGAACTCCGGTTACATGCCTCGTCGTCt                    <  2:1027733/150‑1 (MQ=255)                                                                                                                                   aTGGCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGt                 <  1:57997/151‑1 (MQ=255)                                                                                                                                      gCTCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTg               >  1:547898/1‑150 (MQ=255)                                                                                                                                        tCCATCATCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGAc             >  2:179168/1‑150 (MQ=255)                                                                                                                                          catcatCGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg          <  1:56134/151‑1 (MQ=255)                                                                                                                                           atcatcGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTgg         <  1:147907/151‑1 (MQ=255)                                                                                                                                                cGAAAAGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcgc      >  2:923462/1‑149 (MQ=255)                                                                                                                                                  aaaaGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGa  <  1:51397/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AACCGTGACGGTCAGGAAGAGAAGCAGGTTTCCTTCAACAGCATCTACATGATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGA  >  NC_000913/4187471‑4187766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑