BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,905,639	(A)_9→8	intergenic (‑42/+92)	bglF ← / ← bglG	fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component/transcriptional antiterminator of the bgl operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,905,631	0	A	.	100.0%	512.5 / NA	105	intergenic (‑34/+100)	bglF/bglG	fused beta‑glucoside‑specific PTS enzymes: IIA component/IIB component/IIC component/transcriptional antiterminator of the bgl operon
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (51/54); total (51/54)

Only 100 of 113 total aligned reads displayed.

TGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCAAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATA  >  NC_000913/3905482‑3905776

   gctttgctttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaaa                                                                                                                                               <  1:383851/151‑1 (MQ=255)

     tttgctttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaaaa                                                                                                                                              >  1:867136/1‑150 (MQ=255)

          tttCATCCTTTAATTTAAAACGTAATCGCGTTGTGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaaaaaacctg                                                                                                                                        >  2:616145/1‑147 (MQ=255)

           ttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaaaaaacctg                                                                                                                                        >  1:1070179/1‑146 (MQ=255)

           ttCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCaaaaaaaacctg                                                                                                                                        >  1:1277740/1‑146 (MQ=255)

             cATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGaa                                                                                                                                     <  1:1256928/150‑1 (MQ=255)

                ccTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGaaaaa                                                                                                                                  >  2:824198/1‑150 (MQ=255)

                   ttaatttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAAc                                                                                                                               >  2:92943/1‑150 (MQ=255)

                    taatttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACg                                                                                                                              <  2:1271041/150‑1 (MQ=255)

                     aatttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGc                                                                                                                            <  2:713203/151‑1 (MQ=255)

                      atttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGcc                                                                                                                           >  2:630875/1‑151 (MQ=255)

                        ttaaAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTg                                                                                                                         >  2:746529/1‑151 (MQ=255)

                          aaaaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGa                                                                                                                        <  1:296857/150‑1 (MQ=255)

                          aaaaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGAc                                                                                                                       >  2:778403/1‑151 (MQ=255)

                           aaaCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACg                                                                                                                      <  2:759030/151‑1 (MQ=255)

                            aaCCTAATCGCGTTGCGCAATGCATCAGTCTCCCAATGTTATCTGCGCGCTCGACTCCTTCGACTATTTTTCTGGGTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTTC‑AAAAAAAACCTGAAAAAAACGGCCTGAc                                                                                                                       <  2:1276943/149‑1 (MQ=255)

                             aCGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTg                                                                                                                    <  2:13699/151‑1 (MQ=255)

                              cGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTg                                                                                                                    >  2:1319904/1‑150 (MQ=255)

                              cGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGa                                                                                                                   <  1:372741/151‑1 (MQ=255)

                               gTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGa                                                                                                                   >  1:17338/1‑150 (MQ=255)

                                tAATCGCGTTGCGCAATGCATCAGACCCACAATGATATCTGCGCCCCCGACTCCGGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGaa                                                                                                                  <  1:498276/150‑1 (MQ=255)

                                 aaTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAAt                                                                                                                 <  2:1121524/150‑1 (MQ=255)

                                  aTCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCa                                                                                                               <  2:656656/151‑1 (MQ=255)

                                      cgTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGc                                                                                                            >  1:415446/1‑150 (MQ=255)

                                          gcgcAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtt                                                                                                        <  2:51743/150‑1 (MQ=255)

                                          gcgcAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtt                                                                                                        <  1:1203409/150‑1 (MQ=255)

                                           cgcAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAAtttt                                                                                                      >  1:662446/1‑151 (MQ=255)

                                                  cATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAgg                                                                                               >  1:471597/1‑151 (MQ=255)

                                                   aTCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGt                                                                                              <  1:292474/151‑1 (MQ=255)

                                                   aTCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGt                                                                                              <  2:203684/151‑1 (MQ=255)

                                                    tCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGtt                                                                                             >  2:1058310/1‑151 (MQ=255)

                                                     cAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGtt                                                                                             <  2:517463/150‑1 (MQ=255)

                                                      aGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGtttt                                                                                           <  1:1110939/151‑1 (MQ=255)

                                                      aGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGtttt                                                                                           >  2:248578/1‑151 (MQ=255)

                                                        aCTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGc                                                                                         <  1:315355/151‑1 (MQ=255)

                                                        aCTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGc                                                                                         >  1:631997/1‑151 (MQ=255)

                                                          tCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGccc                                                                                       >  1:746209/1‑151 (MQ=255)

                                                            acaATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGcc                                                                                        >  1:1107613/1‑148 (MQ=255)

                                                             caATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGc                                                                                     >  1:1087261/1‑150 (MQ=255)

                                                              aaTGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGcgt                                                                                   <  1:600575/151‑3 (MQ=255)

                                                                  ttATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTg                                                                               >  1:1251193/1‑151 (MQ=255)

                                                                  ttATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTg                                                                               >  1:720954/1‑151 (MQ=255)

                                                                  ttATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTg                                                                               >  1:515748/1‑151 (MQ=255)

                                                                   tATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGc                                                                              >  2:244218/1‑151 (MQ=255)

                                                                   tATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGc                                                                              >  1:1022376/1‑151 (MQ=255)

                                                                    aTCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGc                                                                              <  2:417595/150‑1 (MQ=255)

                                                                    aTCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCg                                                                             >  1:638007/1‑151 (MQ=255)

                                                                        gcgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCg                                                                             <  2:522445/147‑1 (MQ=255)

                                                                        gcgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGt                                                                           <  1:1210360/149‑1 (MQ=255)

                                                                         cgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAAc                                                                        >  1:279615/1‑151 (MQ=255)

                                                                         cgcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGAGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAAc                                                                        >  2:936167/1‑151 (MQ=255)

                                                                          gcCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAAc                                                                        >  1:293679/1‑150 (MQ=255)

                                                                            ccccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACaa                                                                      >  1:1151088/1‑150 (MQ=255)

                                                                            ccccGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAAt                                                                     <  2:506289/151‑1 (MQ=255)

                                                                                gACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCtt                                                                 <  1:942773/151‑1 (MQ=255)

                                                                                gACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCtt                                                                 >  2:1266958/1‑151 (MQ=255)

                                                                                 aCTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCttt                                                                <  2:573126/151‑1 (MQ=255)

                                                                                    ccTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACt                                                             >  2:431011/1‑151 (MQ=255)

                                                                                    ccTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACt                                                             <  1:1053695/151‑1 (MQ=255)

                                                                                    ccTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACt                                                             >  2:949330/1‑151 (MQ=255)

                                                                                      tGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTc                                                            >  2:1299259/1‑150 (MQ=255)

                                                                                       gCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTc                                                            <  2:934741/149‑1 (MQ=255)

                                                                                       gCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCa                                                           <  2:1006694/150‑1 (MQ=255)

                                                                                       gCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAg                                                          <  2:339513/151‑1 (MQ=255)

                                                                                        cGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                                         <  2:400135/151‑1 (MQ=255)

                                                                                         gACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                                         >  1:577462/1‑150 (MQ=255)

                                                                                           cTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGt                                                         <  1:379170/148‑1 (MQ=255)

                                                                                            tATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaat                                                      >  1:438174/1‑150 (MQ=255)

                                                                                             aTTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaata                                                     >  1:212807/1‑150 (MQ=255)

                                                                                             aTTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaata                                                     >  2:590879/1‑150 (MQ=255)

                                                                                             aTTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGtaat                                                      >  2:576091/1‑149 (MQ=255)

                                                                                                 ttCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATAtt                                                >  2:932400/1‑151 (MQ=255)

                                                                                                 ttCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATAtt                                                >  1:728356/1‑151 (MQ=255)

                                                                                                  tCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATAttt                                               >  2:690217/1‑151 (MQ=255)

                                                                                                    tGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTc                                              >  1:1228365/1‑150 (MQ=255)

                                                                                                       cTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAgtg                                          >  1:433454/1‑151 (MQ=255)

                                                                                                         aaCTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAgtgt                                         <  1:641039/150‑1 (MQ=255)

                                                                                                         aaCTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAgtgt                                         <  1:820885/150‑1 (MQ=255)

                                                                                                         aaCTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGtt                                        >  2:882923/1‑151 (MQ=255)

                                                                                                         aaCTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGtt                                        <  2:1090496/151‑1 (MQ=255)

                                                                                                            tCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCtt                                     >  1:1256848/1‑151 (MQ=255)

                                                                                                               gTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgc                                  >  1:846376/1‑151 (MQ=255)

                                                                                                                tCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgc                                  >  2:51885/1‑150 (MQ=255)

                                                                                                                 cATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTgcg                                 <  2:98685/150‑1 (MQ=255)

                                                                                                                          cccTCTACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGctct                       <  2:812778/151‑1 (MQ=255)

                                                                                                                            ctctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCtat                     >  2:1201271/1‑151 (MQ=255)

                                                                                                                              ctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTatat                   >  2:65712/1‑151 (MQ=255)

                                                                                                                              ctACCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTatat                   >  1:1140094/1‑151 (MQ=255)

                                                                                                                                aCCGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATAtt                  <  1:590985/150‑1 (MQ=255)

                                                                                                                                 ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAGACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATAttt                 <  1:925517/150‑1 (MQ=255)

                                                                                                                                 ccGCTTTGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCAGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTACGCACGCGCTCTATAttt                 <  1:921709/150‑1 (MQ=255)

                                                                                                                                       tGCGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCt           <  1:574547/150‑1 (MQ=255)

                                                                                                                                         cGGCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTaaa        <  2:123088/151‑1 (MQ=255)

                                                                                                                                          ggCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTaaa        >  2:296803/1‑150 (MQ=255)

                                                                                                                                           gCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTaaaaa      <  1:213117/151‑1 (MQ=255)

                                                                                                                                           gCAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTaaaaa      <  1:1239217/151‑1 (MQ=255)

                                                                                                                                            cAAAACTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAAc     <  2:73749/151‑1 (MQ=255)

                                                                                                                                              aaaCTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAACCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACAt   >  2:731154/1‑151 (MQ=255)

                                                                                                                                               aaCTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATa  <  1:599808/151‑1 (MQ=255)

                                                                                                                                               aaCTCC‑AAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATa  <  1:262868/151‑1 (MQ=255)

TGCGCTTTGCTTTCATCCTTTAATTTAAAACGTAATCGCGTTGCGCAATGCATCAGACTCACAATGTTATCTGCGCCCCCGACTCCTGCGACTATTTTTCTGGCTAACTCCGTCATAACTTGCCCTCTACCGCTTTGCGGCAAAACTCCAAAAAAAAACCTGAAAAAAACGGCCTGACGTGAATCAAGCAATTTTTTTCAGGTTTTGCCCGCTTAGTGCGGTAACAATCCTTTACTCAGTAATAATATTTCAGTGTTCTTTGCGCACGCGCTCTATATTTATGGCTAAAAACATA  >  NC_000913/3905482‑3905776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`