Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,659	0	T	A	41.4%	96.0 / 34.4	70	D601V (GAT→GTT)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base T (10/31);  new base A (29/0);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.63e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCCTTCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAG  >  NC_000913/718509‑718807                                                                                                                                                       |                                                                                                                                                      cGGCCTTCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAt                                                                                                                                                       <  2:951766/151‑1 (MQ=255)    ccTTCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCAt                                                                                                                                                    <  2:241580/151‑1 (MQ=255)    ccTTCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCAt                                                                                                                                                    <  2:1221002/151‑1 (MQ=255)      ttCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCGACAAAACa                                                                                                                                                     >  1:366462/1‑148 (MQ=255)          gCATCCGGCAGACGAACCAGTTCCCCTTCACCGCGGAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCAtt                                                                                                                                               <  2:19192/150‑1 (MQ=255)          gCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCa                                                                                                                                                 <  2:416453/148‑1 (MQ=255)           cATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTc                                                                                                                                             <  2:871782/151‑1 (MQ=255)            aTCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATCAAAAACATTCAttt                                                                                                                                              >  2:664857/1‑149 (MQ=255)            aTCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCAGAAAAAACaagttttt                                                                                                                                              >  1:670302/1‑142 (MQ=255)                ggCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGac                                                                                                                                        <  1:906931/151‑1 (MQ=255)                 gCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGAAACAAAAACTGCATTTTCTTac                                                                                                                                        >  2:105753/1‑150 (MQ=255)                 gCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGaca                                                                                                                                       <  1:1258596/151‑1 (MQ=255)                   aGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGGTACAAAACATGCTTTTTCtttcac                                                                                                                                      >  1:259341/1‑145 (MQ=255)                    gACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGAcaca                                                                                                                                     <  1:1008212/150‑1 (MQ=255)                     aCGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAAc                                                                                                                                   <  2:858014/151‑1 (MQ=255)                      cGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACt                                                                                                                                  <  2:1021497/151‑1 (MQ=255)                       gAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATGACTTTTCTGACCCAAcc                                                                                                                                  >  1:625380/1‑149 (MQ=255)                           cAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGc                                                                                                                             <  2:1199500/151‑1 (MQ=255)                             gTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGTGAAACAAAACATATATTTTTTTACACAACTGAccc                                                                                                                             >  1:953469/1‑147 (MQ=255)                               tCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAgg                                                                                                                          <  1:157209/150‑1 (MQ=255)                               tCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAgg                                                                                                                          <  1:1052620/150‑1 (MQ=255)                                 cACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATTATGCATTTTCTTTCACAAATGAAGCAggg                                                                                                                         >  2:855067/1‑149 (MQ=255)                                  aCTTCACCGCGTAAATAGGGGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCGTACGGAACATCTCTTGTTGCAGTTGTATCACGTTGTGGCGGGCATACAAATCATGCATTGCCTGACACAACTGACGCAggg                                                                                                                         <  1:983351/148‑1 (MQ=255)                                   cTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGCTACAAAACATGCATTTCTTGAGACAACTAGCGCCGGGgat                                                                                                                      >  2:907555/1‑150 (MQ=255)                                   cTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCGTAAAAAAAATGCATTTTCTTTCACAAATAACGCAaggg                                                                                                                        >  2:682752/1‑147 (MQ=255)                                       aCCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAACATTCATTTTCTTCCACAACAGACGCCgggggtaaa                                                                                                                   >  1:678255/1‑143 (MQ=255)                                        ccGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCGTACAAATAATGCATTTTCTTGCACAACCGAACCAgggggtaaa                                                                                                                   >  1:706162/1‑142 (MQ=255)                                         cgcgTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCGTACAAAAAATACACTTTCTGTCAAAACTGACGCCgggggtaaa                                                                                                                   >  2:1071440/1‑141 (MQ=255)                                         cgcgTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGc                                                                                                               <  2:74536/151‑1 (MQ=255)                                           cgTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGt                                                                                                             <  2:851511/151‑1 (MQ=255)                                             tAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAAACgg                                                                                                                >  2:1214980/1‑146 (MQ=255)                                              aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCGAAAAAAAATGCATTTTCTTTCAAAAACGACGGCGGGGGTAAACGGCGTAgg                                                                                                          >  1:45799/1‑150 (MQ=255)                                              aaaTAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATTCATTTTCTTACCCAACTTAAGCAGGGGATaa                                                                                                                    >  2:1236811/1‑141 (MQ=255)                                                   ggCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATTCCTTTTCTGACACAAACTACGCAGGGGATAAACGGCGGAgggccc                                                                                                      >  1:598818/1‑148 (MQ=255)                                                        aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTTGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATg                                                                                                <  1:133919/151‑1 (MQ=255)                                                        aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATTCATTTTCTGACACAAATGAAGCAGGGGATAAAAGGCGGAGGGCTCtt                                                                                                    >  2:147789/1‑147 (MQ=255)                                                             gCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATTCATTTTCTTTCACAACATCCGCAGGGGGTAAACCGCGGAGCGCTCTTCTTGCTGtt                                                                                           >  2:612440/1‑151 (MQ=255)                                                               ttcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATTCATTTTCTTTCCCAACAGACGCAGGGGATAACCCGCGGAGAGCTCTTCATGCTGttt                                                                                          >  2:183566/1‑150 (MQ=255)                                                                tcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGTAGGGTATAACCGGCGTAGCGCTCTTCATGCTGttt                                                                                          <  1:682038/149‑1 (MQ=255)                                                                   tGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAAACGGCGGAGCGCTCTTCATGCTGttttt                                                                                        >  2:966153/1‑146 (MQ=255)                                                                    gCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACAAGCCTTTTCTTACACAAATTACGCCGGGGATAAACCGCGTAGCGCTCTTTCTGCTGttt                                                                                          >  2:21463/1‑145 (MQ=255)                                                                           cATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATATAAAAAATGCCTTTTCTTACACAAACAACCCAAGGGATAAACGGCGCTGCGGCCTTTATTCTGTTTGTTGATGGGAgg                                                                              >  2:1040740/1‑150 (MQ=255)                                                                               cTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTAAAAAAAAAGCAATTTCTTACACAAATCAAGCAGGGGGTAAAACGCGTAGCGCCCTTTTTGCTGTTTTTTGATGGAAgg                                                                              >  1:1162152/1‑146 (MQ=255)                                                                                    gcgTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCCAAAAATACTGCATTTTCTTACACAACTCACGCAGGGGATAACCGGCGTAGCGCCCTTCTTGCTGTTTTTTGATggggggaaggaccttt                                                                    >  1:832400/1‑137 (MQ=255)                                                                                        gggAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTa                                                                <  1:195579/151‑1 (MQ=255)                                                                                          gAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAACATGCATTTTCTGACACAAATGACGCCGGGGATAAACCGCGGAGCGCCCTTCATGCTGTTTGTTGATGGGAGGGAGGACTTCTGCtta                                                               >  1:1110443/1‑148 (MQ=255)                                                                                           aaGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATg                                                             <  1:255472/151‑1 (MQ=255)                                                                                                cTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGGCAAAAAAAATTTCTTTTCTGTCCCAACCGACGGAGGGGGGAAACAGCGGAGAGGCCTTTCTGCTGGTTTTAGAAGGAAGGGAGGAAGTCTGCTAATgggg                                                          >  1:740605/1‑147 (MQ=255)                                                                                                  ccTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTc                                                      <  2:575506/151‑1 (MQ=255)                                                                                                    cttccggaCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCg                                                     <  1:958572/143‑1 (MQ=255)                                                                                                     tACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAATGCATTTTCTGACACAACTGACGCAGGGGATAAACGGCGGAGCGCGCTTCATGCTGTTTGTTGATGGGAGGGAGGACTTCTTCTAAATGCGcggcgg                                                    >  2:92837/1‑150 (MQ=255)                                                                                                      aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCTAAAAAATCATTCGTTTTCCCATAAAACTGTCGCAGGGGATAAACCGCGCAGCGCTTCTTATGCGGTTTTTAGAAGGGAAGGAGCACCTTTTCTAATTGCGCGTCgg                                                    >  2:873670/1‑149 (MQ=255)                                                                                                         gAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCa                                              <  1:361756/151‑1 (MQ=255)                                                                                                          aaCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCaa                                             <  2:1273762/151‑1 (MQ=255)                                                                                                           aCATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAg                                            <  2:130933/151‑1 (MQ=255)                                                                                                             aTCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGc                                           <  1:166818/150‑1 (MQ=255)                                                                                                                tctTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATAATGCATTTTCTTACACAAATGACGCAGGGGATAAACGGCGGAGCGCTCTTCCTGCTGTTTGTAGATGGAAGGGAGGACTTCTGCTAATTGGGCGGCGGGC‑TCa                                              >  2:850796/1‑144 (MQ=255)                                                                                                                 ctTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAAAAAGCATTTCCTGACACAAAAGACGCCGGGGGTAAACGGCGGAGCGGTCTTTATGCTGGTTTTAGATGGGAGGGAGGACTTCTGCTAATGGGGCGTCGGAC‑TCAAACAGgtt                                      >  2:399775/1‑151 (MQ=255)                                                                                                                      tGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAACATTCATTTTCTGATACAACTGACGCCGGGGATAAACCGCGGAGCGCTCTTCATGCTGTTTGTAGATGGGAGGGAGCACTTCTGCTAATGGCGCGGCGGGC‑TCAAGCAGgtttt                                    >  1:245167/1‑148 (MQ=255)                                                                                                                       gCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGaa                                <  1:76872/151‑1 (MQ=255)                                                                                                                       gCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGaa                                <  1:231620/151‑1 (MQ=255)                                                                                                                        cAGTTGTTTCACGTTGTGGCGGGGATACAAAACATGCATTTCCTGACACAAACGACGCAGGGGGAAAAAGGCGGAGCGCCCTTCATTCTGTTTGTAGATGGAAGGGAGGACTTCTTCTAATAGCGCGGCGGGG‑TCAAGAAGgtttt                                    >  1:1055293/1‑146 (MQ=255)                                                                                                                        cAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGGATTTTCTGAGACAAACGACGCAGGGGGTAAACGGCGGAGCGCTCTTCATGCTGTTTGTAGATGGGAGGGAGGAATTTTGCTTATTGGGCGGCGGGC‑CCAAGCAGgttttt                                   >  1:483629/1‑146 (MQ=255)                                                                                                                          gttgttTCACGTTGTGGCGGGCATACAAAACATGCATTTCCTTACACCAAATACGCCGGGGATAACCGGCGGAGCGCCCTTCCTGCTGTTTTTTGGTGGGAGGGAGCAACTCTGCTAATGGCGCGGCGGGCTTCAACAGGTTTTTCGag                                >  1:611596/1‑148 (MQ=255)                                                                                                                                   cGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACcag                       <  1:1297403/148‑1 (MQ=255)                                                                                                                                    gTTGTGGCGGGCATACAAATAATGCATTTTCTTCCACAACTGACGCAGGGGGTAAACGGCGGAGCGCCCTTCCTTCTTTTTGTTGATGGAAGGTAGCACTTCCTCTTATTGCGCGCCGGGC‑TCTAGCAGGTTTTTGTAACGTAcccaccgg                   >  1:454482/1‑145 (MQ=255)                                                                                                                                       gtgGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGc                 <  2:215116/150‑1 (MQ=255)                                                                                                                                            gggCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGcaaca             >  2:739516/1‑149 (MQ=255)                                                                                                                                             ggCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGt          >  2:329362/1‑151 (MQ=255)                                                                                                                                                    aaaTCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGa    >  2:162709/1‑150 (MQ=255)                                                                                                                                                     aaTCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAg  <  2:799485/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      CGGCCTTCTGCATCCGGCAGACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGAC‑TCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAG  >  NC_000913/718509‑718807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑