Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,882	0	T	G	45.6%	114.8 / 25.7	80	Y252S (TAC→TCC)	truA	tRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (12/31);  new base G (36/0);  total (49/31)
Fisher's exact test for biased strand distribution p-value = 2.62e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATA  >  NC_000913/2434735‑2435031                                                                                                                                                    |                                                                                                                                                       ggTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAAt                                                                                                                                                     <  2:1219013/151‑1 (MQ=255)  gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGaa                                                                                                                                                      >  1:789775/1‑149 (MQ=255)     cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATacac                                                                                                                                                 >  1:101283/1‑151 (MQ=255)      atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAAAATAACGGGCAGGGGAATcccac                                                                                                                                                >  1:977985/1‑148 (MQ=255)        atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcgc                                                                                                                                              <  2:852227/151‑1 (MQ=255)        atTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACcg                                                                                                                                               <  1:840112/150‑1 (MQ=255)            gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAGCATACCAGGCAGGGGAATTCAcc                                                                                                                                                >  1:27114/1‑145 (MQ=255)            gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGATCATAACGGTCAGGGGAAACCACCCCCAcc                                                                                                                                          >  1:173656/1‑151 (MQ=255)               tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCAAACCGGGCAGGGGAAACCACCGCGAcc                                                                                                                                          >  1:590138/1‑148 (MQ=255)               tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCCGGGGAAACCACCCCCAcc                                                                                                                                          >  1:175576/1‑148 (MQ=255)                  gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCCCGAcc                                                                                                                                          >  1:525237/1‑145 (MQ=255)                       gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGcc                                                                                                                               <  2:1115071/150‑1 (MQ=255)                         aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCtt                                                                                                                            <  2:2874/151‑1 (MQ=255)                         aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTGGGAGATCATAACGGGAAGGGGAATCCACCCCGACCCGATAC‑AAccc                                                                                                                              >  2:688236/1‑149 (MQ=255)                          cTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACGGTCAGGGGAATCCACCCCGACCAGATAC‑AGCCCtt                                                                                                                            >  1:125694/1‑150 (MQ=255)                          cTCGTCATCTTTCAGGCCGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGGAATCCACCGCGACCAGATAC‑AGCCCtt                                                                                                                            >  2:839180/1‑150 (MQ=255)                            cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTc                                                                                                                           <  2:934145/149‑1 (MQ=255)                               cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCt                                                                                                                       <  2:1299591/150‑1 (MQ=255)                               cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCt                                                                                                                       <  2:255277/150‑1 (MQ=255)                                    ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCttt                                                                                                                     <  2:585378/147‑1 (MQ=255)                                    ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGc                                                                                                                  <  1:1135655/150‑1 (MQ=255)                                    ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AACCCTTCCGCTTTTGcc                                                                                                                 >  2:341332/1‑151 (MQ=255)                                     tCAGGCTGTAACTACGTTGGCTACAGCTTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGcc                                                                                                                 <  2:1081278/150‑1 (MQ=255)                                     tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGcc                                                                                                                 <  1:1286450/150‑1 (MQ=255)                                      cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGt                                                                                                               <  1:624648/151‑1 (MQ=255)                                         gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTtgc                                                                                                            <  2:663409/151‑1 (MQ=255)                                         gCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTTCCGtt                                                                                                              >  2:951196/1‑149 (MQ=255)                                          cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATACCGGGCAGGGGAATCCACCCCCCACAGATACAACCCCTTCCCCtttttcggt                                                                                                               >  1:1249689/1‑143 (MQ=255)                                          cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAAACCACCCCGACCAGATAA‑AACCCTTCCGCTTTTTCCGtt                                                                                                              >  2:41065/1‑148 (MQ=255)                                           tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAAACCACCGCGACCAGATAC‑AACCCTTCCCCCTTTTCCggt                                                                                                              >  1:1058539/1‑145 (MQ=255)                                           tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATAATAACGGTCAGGGGAATCCAACGCGACCAGAAAC‑AGCCCTTCCGCTTTTGGCGGtgct                                                                                                           >  2:959630/1‑150 (MQ=255)                                            gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTtgct                                                                                                           <  2:893620/149‑1 (MQ=255)                                                 tACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAAACCACCGCGACCAGATAC‑AACCCTTCCGCTTTTGCCGTTTCTgccgcc                                                                                                     >  2:1238242/1‑150 (MQ=255)                                                  aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AACCCTTCCGCTTTTGCCGTTGCTgccccc                                                                                                     >  2:429780/1‑149 (MQ=255)                                                   cGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTCTACCTGGCCGGGCGATCAAACGCGGCAAGATAG‑AGCCCCTCCCCTTTTGCCCAtgctgtcccc                                                                                                     >  2:731371/1‑143 (MQ=255)                                                    gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCCCTTTTTCCGTTTCTGCCGCCcgcg                                                                                                 >  2:769056/1‑151 (MQ=255)                                                     ttGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATTCCCGGCCGGGGAAAACAACCCGACCCAAAAA‑AGCCCTTCCCCCTTTTCCGCTTGTGCCCCCcgcg                                                                                                 >  1:567905/1‑150 (MQ=255)                                                      tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGTAATCCACCGCGACCCGATAC‑AGCCCCTCCGCTTTTGCCGTTGGTGCCCCCAGCggt                                                                                               >  1:160380/1‑149 (MQ=255)                                                       ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGtt                                                                                               <  1:438413/150‑1 (MQ=255)                                                        gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCt                                                                                             <  2:1334144/151‑1 (MQ=255)                                                        gCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCt                                                                                             <  1:1205211/151‑1 (MQ=255)                                                              gCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCtt                                                                                       <  1:48388/151‑1 (MQ=255)                                                                    aaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGGCCAGATAC‑AGCCCTTCCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTgcc                                                                                   >  2:1085362/1‑149 (MQ=255)                                                                      tGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGTCAGGGTAATCCACCGCGACCAGATAA‑AGCCCCTCCGCTTTTTCCGTTGCTGCCCCCCGCGTTCTGTCCTTTtccgcc                                                                                >  2:225741/1‑150 (MQ=255)                                                                            gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCCGGGGAATCCACCGCGGCCAGATAC‑AGCCCCTCCGCCTTTGCCGTTGCTGCCCCCCGCGGTCTGTCCCTTGCCGCCCGCAAc                                                                          >  1:977931/1‑150 (MQ=255)                                                                            gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAACATACCGGGCAGGGGAATCCACCGCGACCAGATAA‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCAGCaa                                                                           >  2:1150757/1‑149 (MQ=255)                                                                            gAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTCGAAAAACATTTCCGGCAGGGGAAACCACCCCGAACCAATAA‑AGCCCCTCCCCCTTTTCCGTTTCTGCCGCCAGCGTTCTGGCCTTTgccccc                                                                                >  2:974381/1‑144 (MQ=255)                                                                               cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGCAGATCATACCGGGCAGGGGAATCCACCGCGCCCAGATAC‑AGCCCCTCCCCTTTTGCCGTTGCTGCCGCCAGCGCTCTGTCCTTTtccgcc                                                                                >  1:1278616/1‑141 (MQ=255)                                                                                    ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCCCGACCCGATAC‑AGCCCTTCCGCCTTTTCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCAGCAACTCTTCTAt                                                                  >  2:1264270/1‑150 (MQ=255)                                                                                     tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCGGGGGAATCCACCGCCACCCGAAAC‑AGCCCCTCCCCCTTTTCCGGTGCTTCCGCCCGCGTTCTGTTCTTTGCCGCCCGCCActct                                                                       >  1:836990/1‑144 (MQ=255)                                                                                      ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCCCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTATcc                                                                >  2:711960/1‑150 (MQ=255)                                                                                         gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATACCCCCCGCCCAGATAC‑AGCCCCTCCCCTTTTTCCGGTGCTGCCGCCAGCGGTCTGTTCTTTGCCCCCAGCCACTCTGCTATCCAGc                                                             >  1:699625/1‑150 (MQ=255)                                                                                           tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAACCACCGCGACCAAATAC‑ACCCCTTCCGCTTTTGCCCTTGCTGCCGCCAGCGTTCTGTCCTTTTCCGCCAGCAACATTGCCATCCCGctct                                                          >  2:1139617/1‑151 (MQ=255)                                                                                           tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctc                                                           <  1:1267642/150‑1 (MQ=255)                                                                                            aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCGGGTAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCCCCTTTGCCGTTTCTGTCCCCAGCGGTCTGTCCTTTGCCGCCCGCAACtcttctcttta                                                               >  1:528528/1‑140 (MQ=255)                                                                                             gTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACATACCGGGCCGGGGAAACCAACGCGAACAGATAC‑AACCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTTTGTCCTTTGCCGCCAGCAACACTGCCATCCCGCCCTcc                                                        >  2:1233100/1‑151 (MQ=255)                                                                                                      aaaTAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTTCTATCCAGCTCTCCGGGTgggt                                                >  2:287637/1‑148 (MQ=255)                                                                                                      aaaGAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                                <  1:1089288/150‑1 (MQ=255)                                                                                                       aaTAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATAA‑AGCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                                >  2:273299/1‑149 (MQ=255)                                                                                                           gCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTggg                                           <  1:1047553/150‑1 (MQ=255)                                                                                                             gggCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGTAATCCACCCCGACCCGATAC‑AGCCCCTCCCCTTTTGCCGGTGCTGCCGCCCGCGCTCTGTCCTTTTCCGCCCGCAACCCTGCTCTTCAGCTCTCCGGCTGGTTTTggg                                           >  1:784407/1‑148 (MQ=255)                                                                                                               gCCCATTGGCGGTTTTGGAAGGTCATAACCGGCAGGGGAATCCACCGCGACCCGATAC‑AGCCCTTCCCCTTTTGCCGTTTCTGCCCCCAGCGCTCTGTCCCTTTCCGCCCGCAACTCTTCTATTCAGCTCTCCGGCTGGGTTTGGGGAc                                        >  1:643667/1‑149 (MQ=255)                                                                                                                      ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc                                >  2:344085/1‑150 (MQ=255)                                                                                                                         ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCa                            <  2:102084/151‑1 (MQ=255)                                                                                                                          gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                             >  1:1200246/1‑149 (MQ=255)                                                                                                                          gTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                             >  2:219536/1‑149 (MQ=255)                                                                                                                              tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                        <  2:926458/150‑1 (MQ=255)                                                                                                                               ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg                       <  2:318718/150‑1 (MQ=255)                                                                                                                               ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc                      >  2:102232/1‑151 (MQ=255)                                                                                                                                gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                     >  1:297630/1‑151 (MQ=255)                                                                                                                                 aaGATCATACCGGTCAGGGTAATCCACCGCGACTAGATAC‑AGCCCTTCCGCTTTGGCTGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACCTCCATCAGGCTGc                      >  1:946263/1‑149 (MQ=255)                                                                                                                                  aGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg                    >  2:530087/1‑150 (MQ=255)                                                                                                                                          ccGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt            <  1:712920/150‑1 (MQ=255)                                                                                                                                          ccGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc           >  2:124335/1‑151 (MQ=255)                                                                                                                                            ggTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt         <  1:50529/151‑1 (MQ=255)                                                                                                                                            ggTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCt         >  1:646010/1‑151 (MQ=255)                                                                                                                                             gTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg        <  1:55648/151‑1 (MQ=255)                                                                                                                                             gTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg        <  2:558669/151‑1 (MQ=255)                                                                                                                                             gTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCATg        <  2:593720/151‑1 (MQ=255)                                                                                                                                                   gTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCata  <  1:287864/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                       GGTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAC‑AGCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCATA  >  NC_000913/2434735‑2435031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑