Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	41.9%	86.6 / 45.9	74	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (9/34);  new base T (31/0);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 7.39e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206294‑3206585                                                                                                                                              |                                                                                                                                                         tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAGTATTTTAGACaaa                                                                                                                                                  >  1:908124/1‑150 (MQ=255)   gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACcaaaa                                                                                                                                                >  2:753329/1‑148 (MQ=255)    cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaaaa                                                                                                                                                >  2:959353/1‑147 (MQ=255)     aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaa                                                                                                                                             <  1:1340420/151‑1 (MQ=255)      aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACcaaaaaaa                                                                                                                                             >  2:1191954/1‑145 (MQ=255)       aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaa                                                                                                                                           <  2:86841/151‑1 (MQ=255)       aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaa                                                                                                                                           <  2:53919/151‑1 (MQ=255)                 ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑Gtt                                                                                                                                 <  2:1046535/150‑1 (MQ=255)                 ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑Gtt                                                                                                                                 <  1:393063/150‑1 (MQ=255)                  tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATgg                                                                                                                                      >  1:941987/1‑145 (MQ=255)                    ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCa                                                                                                                             <  1:840657/151‑1 (MQ=255)                       gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGG‑GGTGcc                                                                                                                              >  2:12233/1‑147 (MQ=255)                       gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatga                                                                                                                          <  2:1166617/151‑1 (MQ=255)                       gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatg                                                                                                                           <  1:1114241/150‑1 (MQ=255)                       gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCatg                                                                                                                           <  1:517911/150‑1 (MQ=255)                          ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAACAAAACAAAAACTCGA‑GTTGGCatgat                                                                                                                         >  1:495073/1‑149 (MQ=255)                           tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGAATTTTTCAAACAAAAACAAAAAATCGA‑gcttaccagcttcg                                                                                                                      >  2:1244723/1‑138 (MQ=255)                            ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCAtgatga                                                                                                                       <  2:1105865/149‑1 (MQ=255)                            ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑g                                                                                                                     <  1:326008/150‑1 (MQ=255)                               gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAAAAAAAAAAGGG‑GTTGGCATGATGA‑GCg                                                                                                                   >  2:883795/1‑149 (MQ=255)                               gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCCGACaaaaacaaaaaccagaggt                                                                                                                                  >  1:549082/1‑129 (MQ=255)                                cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAACTGGA‑GTTGCCATGATGA‑GCGaa                                                                                                                 >  2:551298/1‑150 (MQ=255)                                cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GAGaaa                                                                                                                <  2:391056/151‑1 (MQ=255)                                    attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGA‑GTTGCCATGATGA‑GCGaaa                                                                                                                >  2:971137/1‑147 (MQ=255)                                      tcattcTTAGTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCTAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATGCAAAAACTGGA‑GTTGCCATGATGC‑GAGAACTtaat                                                                                                           <  1:727421/150‑1 (MQ=255)                                          tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGc                                                                                                       <  1:1205360/150‑1 (MQ=255)                                               ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAATGGA‑GTTGCCATGATGA‑GCGAAAGAAATAAGAAACa                                                                                                   >  1:336218/1‑149 (MQ=255)                                                   cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAAAAAAAAAAAAAATGGA‑GGTGCCATGATGA‑GCGAAAAAAATAAACaaaaag                                                                                                 >  2:1299371/1‑143 (MQ=255)                                                        tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTTGCC‑TGATGA‑GCGGAAGGAATAAACAAAAAGCAGTg                                                                                            >  1:363222/1‑147 (MQ=255)                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                       <  1:666013/151‑1 (MQ=255)                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                       <  2:56877/151‑1 (MQ=255)                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                        <  2:1254555/150‑1 (MQ=255)                                                         gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCACACAAAAACAAAAAATGGA‑GGTGGCATGATGA‑GCGAAAGTAATAAACAACAGGCAGGGAATa                                                                                        >  1:616635/1‑150 (MQ=255)                                                              aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAAATGGA‑GTTGCCATGATGA‑GCGGAAGTAATAAACAACAGGCAGTGAATAAAtt                                                                                    >  2:528995/1‑149 (MQ=255)                                                               cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCTACACAAATACAAAAACTTGC‑GCTGTCGTCCTTT‑GCGGAAGAAATAAGCAAAACGCAGGGAAAAAAATGAc                                                                                 >  2:997628/1‑151 (MQ=255)                                                                  aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGA‑GTTGCCATGATGA‑GGGAAAGTAATAAACAACAAGAAGTGAATAAGtt                                                                                    >  1:1166195/1‑145 (MQ=255)                                                                        aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                         <  1:275260/150‑1 (MQ=255)                                                                        aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATAAAAAAAATGGA‑GTTGGCAAGATGA‑GGGGAAGGAATAAACAAAAAGCAGTGGAAAAATTggaagaaaat                                                                          >  1:335684/1‑140 (MQ=255)                                                                         aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                                       <  1:1314285/151‑1 (MQ=255)                                                                           tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAACTGGA‑GTTGTCATGGTGA‑GGGAAAGGAAAAAGCAACAGGCAGTGAATAAAATGACAGAGATTGTCg                                                                      >  2:94427/1‑150 (MQ=255)                                                                           tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAAAAGG‑GGTGCCCTGATGA‑GAGAAAATAATAAAAAAAAAGGAGTGAATAAATTGACAGAGATTGt                                                                        >  1:717845/1‑148 (MQ=255)                                                                              atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTaa                                                                  <  2:1212383/151‑1 (MQ=255)                                                                               tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAATAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTaa                                                                  >  2:418307/1‑150 (MQ=255)                                                                                  tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACtt                                                               <  2:673671/150‑1 (MQ=255)                                                                                     cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GTTGCCCTGATGA‑GCGAAAGGAATAAACAACAAGCAATGAATAAATTGACAGAGAATGTTGCTAAATTTAcc                                                           >  2:375870/1‑151 (MQ=255)                                                                                     cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                           <  2:246728/151‑1 (MQ=255)                                                                                      ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                          <  1:930276/151‑1 (MQ=255)                                                                                      ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                                          <  2:783579/151‑1 (MQ=255)                                                                                            aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATAAAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGa                                                    <  1:649932/151‑1 (MQ=255)                                                                                             aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                                   <  1:25155/151‑1 (MQ=255)                                                                                               aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACACAAAAAAAAAAAAATGA‑GTTGGCATGATGA‑GCGGAAGAAATAAAAAACAGGCAGGGGATAAATTGACAGAGAATGTCGCTAACTTTACCCCCATGAttt                                                 >  1:683205/1‑151 (MQ=255)                                                                                                aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTc                                                <  2:930059/151‑1 (MQ=255)                                                                                                  tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGA‑GGTGCCCTGATGA‑GCGGAAGGAATAAACAACAGGCAGGGAATAAATTGACAGAGATTGTCGCCAACTTTACCGCCATGAttttt                                               >  2:458911/1‑148 (MQ=255)                                                                                                   ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACGGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTa                                              <  2:798679/150‑1 (MQ=255)                                                                                                    taAAACCACATTTTTAGTGGTAGTTTGTGGCGGCTAATTTTTTTCAACCAATAAAAAAAATCGAGGTTGCCACG‑TGAGGGGAATGTAAAAAG‑AAAAAGCAGAGAAAAAGGTTAAAGAAGTTGTTGCTAACCTTTACGCCCTTATTTCtt                                              >  1:1138448/1‑148 (MQ=255)                                                                                                       aaCCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAAACAAATACAAAAAATGGA‑GGTGCCATGGTGA‑GCGAAAAGAAAAAACAAAAAGCCGTGAATAAGGTGACAGAGATTGTCGCTAACTTTTCCGCCCTGATTTTTAccc                                           >  2:1144622/1‑149 (MQ=255)                                                                                                             aTTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATACAAAAAATGGG‑GGTGCCATGATGA‑GGGAAAGGAATAAGCAAAAGGCAGTGGATAAGGTGGCAGAGAATGTCGCTAACTTTACCGCCATGATTTTTACCCGAATGcc                                    >  2:383993/1‑150 (MQ=255)                                                                                                              tttttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAATAATAAGCAAAAGGCAGGGGATAAGTTGACAGAGAATGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc                                    >  2:631291/1‑149 (MQ=255)                                                                                                                 ttAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGACAAAAAAAAAAAATGGA‑GGTGGCATGATGA‑GCGGAAGTAATAAACAAAAGGCAGGGAAAAAAATTAAAGAGATTGTTGCTAACTTTAACGCCCTGATTTTTACCCCAAAACCtggt                                >  1:1126143/1‑148 (MQ=255)                                                                                                                      ggTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGA‑GGTGGCCTGATGA‑GCGAAAGTAATAAACAAAAAGCAGTGAAAAAAATGAAAGAGAATGTCGCTAACTTTACCGCCCTGATTTTTTCCCGAAAGGCTGATGACgtg                          >  1:489419/1‑151 (MQ=255)                                                                                                                      ggTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGG‑GGTGCCCTGATGA‑GCGAAAGTAATAAAAAACAGGCAGGGAAAAAATTGACAGAGAATGTCGCTAACTTTAACGCCATGATTTTTACCCGAATTCCTGATGACgtg                          >  2:676789/1‑151 (MQ=255)                                                                                                                         aGTTTGTGGCGGTAATTTTTTCCAAACAAATAAAAAAACTGGA‑GTTGCCATGATGA‑GGGAAAGTAAAAAGCAACGGGCATTAAAAAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtgg                         <  2:1190564/149‑1 (MQ=255)                                                                                                                             tgtgGGGGTAAATTTTACAAAACAAATAAAAAAACGGGG‑GTGGCCATGATGA‑GCAAAAGAAATAAGCAACAGGCAGTGAAAAATTTAACAGAGATTGTCCCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                    <  1:1210684/150‑1 (MQ=255)                                                                                                                               tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                  >  2:1013048/1‑150 (MQ=255)                                                                                                                                ggCGTTGAATTTTTCCAGACAAATAAAAAAACGGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa                 <  1:1160407/150‑1 (MQ=255)                                                                                                                                ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc                >  1:11657/1‑151 (MQ=255)                                                                                                                                   ggTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa              >  1:760109/1‑150 (MQ=255)                                                                                                                                     tGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa            >  2:89992/1‑150 (MQ=255)                                                                                                                                      gAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc          >  1:903806/1‑151 (MQ=255)                                                                                                                                       aaTTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa           <  2:860419/149‑1 (MQ=255)                                                                                                                                         tttttCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg        <  2:396044/150‑1 (MQ=255)                                                                                                                                          ttttCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      >  2:1111178/1‑151 (MQ=255)                                                                                                                                           tttCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      >  1:284560/1‑150 (MQ=255)                                                                                                                                           tttCCAGAAAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      <  2:770979/150‑1 (MQ=255)                                                                                                                                            tgccAGACAATGACAAAAACTGGA‑GTTGCCTAAAGGA‑GGAAAATTAATAACCACCAGGGATATAATAAGTTGCCAGTGATTGTCGCTCACTTGTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      <  1:1020742/147‑1 (MQ=255)                                                                                                                                              ccAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:284039/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                         TCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGA‑GTTGCCATGATGA‑GCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206294‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑