Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,8020AC43.7% 147.6 / 20.4 88V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
Reads supporting (aligned to +/- strand):  ref base A (44/5);  new base C (0/38);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.45e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCT  >  NC_000913/4378653‑4378951
                                                                                                                                                           |                                                                                                                                                      
ccccTCGAGCAATAGCGTCGCCACCAGGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTa                                                                                                                                                        >  2:934501/1‑150 (MQ=255)
ccccTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTa                                                                                                                                                        <  2:910619/150‑1 (MQ=255)
  ccTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccg                                                                                                                                                    <  1:1180369/151‑1 (MQ=255)
  ccTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccg                                                                                                                                                    >  1:1293238/1‑151 (MQ=255)
      gAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgccac                                                                                                                                                >  2:117394/1‑151 (MQ=255)
         cAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgccaccgc                                                                                                                                             <  1:424296/151‑1 (MQ=255)
         cAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgccaccgc                                                                                                                                             >  1:1320931/1‑151 (MQ=255)
                  cGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                                                                    >  1:248766/1‑151 (MQ=255)
                   gCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCggg                                                                                                                                   >  2:1076031/1‑151 (MQ=255)
                     caccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGAt                                                                                                                                 >  1:750403/1‑151 (MQ=255)
                           gCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTttg                                                                                                                           >  2:1260669/1‑151 (MQ=255)
                            cACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTttgt                                                                                                                          >  2:752758/1‑151 (MQ=255)
                              cGCCAGAAATTGTTTTGCTGACCGAACCTACCTCAAACACCTTTTGTTGTGGGACGGGCTGTTTTTGGGCGATGTCCGCATACCCCCAGGTAAAGTAATAAGTTTTACCCTGAAAAATTCCCCCCACCGCCATACCCGGGATCTTttg                                                                                                                           <  2:346453/148‑1 (MQ=255)
                                ccATTAAATTTTTTGCTGCCCGACCCTACCTAAAACAGCTTTTGCTGTGTGCGGGGCTGTTTTTTGGCGATGCCCGCATACCCCCAGGAAAAGTAAAAGGTTTTCCCTTAATAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGct                                                                                                                      <  2:196966/151‑1 (MQ=255)
                                ccAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGct                                                                                                                      >  1:516425/1‑151 (MQ=255)
                                   gTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGctct                                                                                                                    >  2:922752/1‑150 (MQ=255)
                                     aatttttttGCTGCCCAACCCTAACTAAAACACCTTTTGCTGTGTGGCGGGCTGTTTTTGGGCGTTGCCCGCATGCCCCCGGGAAAAAAAAAAGGTTTTCCCCTAAAAATTCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCta                                                                                                                   <  2:1201896/144‑1 (MQ=255)
                                     aattGTTTTGCTCACCGAACCAAACACAAACAGCTTTTGCTGTGTGGCGGGTTGTTTTTTGGGGATCCCCCCATCCCCCCAGAAAAAATAATAAGTTTTCCCTTAATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGctct                                                                                                                    <  1:376252/145‑1 (MQ=255)
                                     aaaTTTTTTGCTGCCCAAACTTACCACAAACATCTTTTGTTGTTGGGCGGTTTTTTTTTTGGGGATGCCCGCATCCCCCCAGGTAAAGTAATAAGTTTTCCCTTGATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCta                                                                                                                   <  1:1149160/149‑1 (MQ=255)
                                     aaaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGc                                                                                                                       >  1:1279921/1‑145 (MQ=255)
                                     aaaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                                                                  >  2:321888/1‑150 (MQ=255)
                                       aTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtata                                                                                                                 >  1:14636/1‑149 (MQ=255)
                                          ttttGCGGCCCAACCCAAACTAAAACAACTTTTGCTGTGTGAGGGGCTTTTTTTGGGCGATGTCCGCATACCCCCAGGAAAAGTAATAGGTTTTCCCCTGAAAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc                                                                                                              <  2:12043/149‑1 (MQ=255)
                                           tttGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCggg                                                                                                           >  2:72600/1‑151 (MQ=255)
                                                tGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAAt                                                                                                      >  2:1076358/1‑151 (MQ=255)
                                                tGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAAt                                                                                                      >  2:880238/1‑151 (MQ=255)
                                                cgACCCAACCGTACCTCAAAAACCTTTTGTTGGGTGGGGGGCTTTTTTTGGGCGATTCCCGCACCCCCCAGGGTAAAGTAAAATGTTTTCCCCTAAAAATTCCCGGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTa                                                                                                        <  1:492706/149‑1 (MQ=255)
                                                   ccGACCCAAACTAAAACAACGTTTGCGGTGTGAGGGTCTGTTTTTGGGCGATGCCCTCATGCCCCAAGAAAAAGTAATAAGTTTTCCCCTAATAATTTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTa                                                                                                        <  1:970622/146‑1 (MQ=255)
                                                   ccGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtgt                                                                                                   >  1:1016186/1‑151 (MQ=255)
                                                    cGACCCAAACTCAAACAACTTTTGTTGTGGGCGGGGTTTTTTTTGGGCGATGCCCGCATACCCCCAGAAAAAGTAAAAGGTTTTCCCATGAAAAATCCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTaa                                                                                                       <  2:412064/146‑1 (MQ=255)
                                                      acccTAACAAAAACAACGTTGTGTGTGTGACGGGTTGTTTTTGGGTTATGCCAGTATCCCCCATGGTAAAGAAAAAATGTTTCCCCAAATAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCg                                                                                                <  1:694024/149‑1 (MQ=255)
                                                           tAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGc                                                                                            >  1:1154054/1‑151 (MQ=255)
                                                            acccaaaaCACCTTTTGTTTTGGGGCGGGCTGTTTTTGGGTGATGTCCGCATGCCCCAAGGAAAATAAAAAGGTTTTCCCTTAAAAAATTCCCCCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGc                                                                                            <  2:161490/145‑1 (MQ=255)
                                                                  accactgTTTGCTGTGGGCGGGGCTTTTTTTTGGCGATCCCCGAATACCCCCAGAAAAATAAATAATTTTTCCCTTAATAAATTCCCCCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAatat                                                                                     <  1:622395/145‑1 (MQ=255)
                                                                  accaacGTTTGTTGTGTGAGGGTTTGTTTTTTGCCAATGCCCGAATCCCCCTAGGAAAAGTAATAGGTTTTCCCTTAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAatat                                                                                     <  2:228860/149‑1 (MQ=255)
                                                                  aacatcTTTTGCTGGGTGGCGGGCTTTTTTGTGGCGATCCCCAAAAAGCCCCTGAAAAAGTAATAAGTTTCCCCCTGATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAatat                                                                                     <  1:350072/151‑1 (MQ=255)
                                                                   acaagtttggCTGTGTGACGGCTTGTTTTTTGGGAATGCCCGAATCGCCCCAGGAAAATTAAAAGGTTTTCCCTTGAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGcaca                                                                                         <  1:586222/137‑1 (MQ=255)
                                                                    caacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCg                                                                                   >  1:899669/1‑151 (MQ=255)
                                                                     aacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGt                                                                                  >  1:659307/1‑151 (MQ=255)
                                                                       cGTTTGCTGTGTGACGGGCTGCTTTTTGGGGATGTCCGCATAGACCCAGGCAAAGTACTAAGGCTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGtt                                                                                 >  1:1156246/1‑150 (MQ=255)
                                                                        gTTTGGTGTGTGGCGGTCTGTTTTTTGGCTTTGCCCGCATGGCCCGGGAAAAAGTAATATGGTTTCCCCTAATAAATCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGtt                                                                                 <  2:631059/149‑1 (MQ=255)
                                                                          ttGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAtt                                                                             >  2:640886/1‑151 (MQ=255)
                                                                          ttGCTGTGAGGCGGGCTTTTTTTTGGGAATGCCCGAATAGCCCCTGTTAAAGTAATAAGGTTTCCCGTGAAAATTGCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAt                                                                              <  2:1273314/150‑1 (MQ=255)
                                                                          tgggtggggggcGGGCTCTTTTTTGGCGATCCCCGCTGACCCCCAGAAAATGTAATGTGGTTTCCCTTGAAAAATCGCCCCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTg                                                                                <  1:496982/137‑1 (MQ=255)
                                                                           tggtgtgtgAGGGGCTTCTTTTTGGCGATGCCCGAATACCCCAGGGTAAATAAATAGGGTTTCCCCTAATAATTTACCGCAACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAt                                                                              <  2:190504/146‑1 (MQ=255)
                                                                           tGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAttt                                                                            >  2:328761/1‑151 (MQ=255)
                                                                              tgtgtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttgtt                                                                         >  2:1053273/1‑151 (MQ=255)
                                                                               gtgtgACGGGTTTTTTTTGGGCGATGTCCGAATAGCCCCAGAAAAAGTAAAAGGTTTTCCCCTAAAAATTTCCCGCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAt                                                                              <  1:1245198/145‑1 (MQ=255)
                                                                                   ggcggttttttttttGGCGATGCCCGCATACCCCCAGGAAAAGAAATAGTGTTTCCCCTAAAAATTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAggg                                                                    <  1:617205/141‑1 (MQ=255)
                                                                                   gcgggttttttttttGGTTATCTCCCCATACCCCCTGAAAAGATAATATTTTTTCCCTTGATAAATTCCCCCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAg                                                                      <  1:634045/139‑1 (MQ=255)
                                                                                   gACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAggg                                                                    >  2:947691/1‑151 (MQ=255)
                                                                                    aCGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAgggg                                                                   >  2:750523/1‑151 (MQ=255)
                                                                                     cGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgc                                                                  >  1:1006924/1‑151 (MQ=255)
                                                                                      ggtcttttttttGGCGATGTCCGCAGACCCCCAGATAAAGTAATAAGTTTTACCTTAAAAATTTCCCGCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAggg                                                                    <  1:293111/141‑1 (MQ=255)
                                                                                      ggtcttttttttGGCGATGCCCGCATACCCCCAGTAAAAGAAATAAGGTTTCCCCTGATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttg                                                                           <  1:695254/134‑1 (MQ=255)
                                                                                      gggCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgc                                                                  >  2:1278652/1‑150 (MQ=255)
                                                                                      gggCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgc                                                                  >  2:1130516/1‑150 (MQ=255)
                                                                                           gctTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCaa                                                             >  2:1252255/1‑150 (MQ=255)
                                                                                             ttttgGGCAAGGTCCTCAGAGCCCCAGTAAAACTAAAATGTTTTACCTTAAAAATTTCCCGCCTCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAAt                                                           <  1:757512/150‑1 (MQ=255)
                                                                                                 ggggaatgccccaaaaGCCCCAGGAAAAAAAAAAAGTTTTCCCTTAAAAATTCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTgg                                                       <  1:66665/135‑1 (MQ=255)
                                                                                                  ggCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTCCCCTGATAAATTACCGCCACCCCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGa                                                      <  2:930810/150‑1 (MQ=255)
                                                                                                   gCGATGTCCGCATACCCCAAGGTAAATAAAAAGGTTTTCCCCTGAAAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGc                                                    <  2:1190235/151‑1 (MQ=255)
                                                                                                   gCGATGCCCGCATCCCCCCGGGAAAAGTAATAAGTTTTCCCTGAATAAATCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGc                                                    <  2:1331774/151‑1 (MQ=255)
                                                                                                        gTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTAAGGGGCAGCAAATGTGGAGCAag                                                 >  1:288610/1‑149 (MQ=255)
                                                                                                          ccgaatgccccgAGGAAAATTAAAAAGTTTCCCCCTAAAAAATTCCCGCCCCCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGc                                             <  2:470773/143‑1 (MQ=255)
                                                                                                          ccGAATACCCCCAGTTAAAATAATAGGTTTACCCCTATAAATTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAaga                                                <  2:957610/148‑1 (MQ=255)
                                                                                                           cGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCg                                            >  1:1173808/1‑151 (MQ=255)
                                                                                                              aTAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGt                                          >  2:321983/1‑150 (MQ=255)
                                                                                                              aTAGCCCCAGGAAAAGTAATAGGTTTTCCCCTAATAAATTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGt                                          <  2:1090649/150‑1 (MQ=255)
                                                                                                                      aGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATtaata                                  >  2:219392/1‑150 (MQ=255)
                                                                                                                       ggaaaaGAAATAGGGTTTCCCCGAAAAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAg                                <  1:52462/148‑1 (MQ=255)
                                                                                                                       ggTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATtaata                                  >  1:166236/1‑149 (MQ=255)
                                                                                                                          aaaGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGgc                              >  1:816196/1‑150 (MQ=255)
                                                                                                                           aaattaaaaGGTTTTCCCTTGATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGt                                          <  1:916834/131‑1 (MQ=255)
                                                                                                                               aatagGTTTTCCCCTAAAAAATCACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCagag                        <  1:1168906/151‑1 (MQ=255)
                                                                                                                               aatagGTTTTACCCTAAAAAATCCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCagag                        <  1:433620/151‑1 (MQ=255)
                                                                                                                               aataaGTTTTCCCCTAATAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCa                           <  1:1330040/148‑1 (MQ=255)
                                                                                                                               aataaGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCag                          >  1:1141658/1‑149 (MQ=255)
                                                                                                                                    ggTTTCCCTTAAAAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcgtc                    <  2:777868/150‑1 (MQ=255)
                                                                                                                                    ggTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAgcggcg                   >  1:1096069/1‑151 (MQ=255)
                                                                                                                                       tttACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGtt                 >  2:523438/1‑151 (MQ=255)
                                                                                                                                        ttACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGctt                >  1:660230/1‑151 (MQ=255)
                                                                                                                                         tttcccttaaaaaaTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGcgtc                    <  2:221886/137‑1 (MQ=255)
                                                                                                                                           cccTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTg              >  2:855045/1‑150 (MQ=255)
                                                                                                                                           cccTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGCGGTAATTGTGCGATGCACAATATCGTTGCTTTGTTGAGGGGCAGCAAATGTGGCGCAAGAGGCGGTAATTAATAATGCGCAAAGGGCCGtt                 >  1:1061390/1‑147 (MQ=255)
                                                                                                                                           cccTAATAAATTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTg              <  2:807464/150‑1 (MQ=255)
                                                                                                                                            ccTTATAATTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTg              <  2:209154/149‑1 (MQ=255)
                                                                                                                                                  aaaaaTTCCCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGaa            <  2:841327/145‑1 (MQ=255)
                                                                                                                                                  aaaaaTTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACa          <  2:248800/147‑1 (MQ=255)
                                                                                                                                                  aTAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACAt         >  2:938964/1‑150 (MQ=255)
                                                                                                                                                  aTAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACAt         >  2:240812/1‑150 (MQ=255)
                                                                                                                                                   tAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACAt         >  2:1091845/1‑149 (MQ=255)
                                                                                                                                                    aaaTTCCCCCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAg       <  2:353426/150‑1 (MQ=255)
                                                                                                                                                    aaaTTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAgg      >  2:237660/1‑151 (MQ=255)
                                                                                                                                                         tACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCt  >  2:1216137/1‑151 (MQ=255)
                                                                                                                                                           |                                                                                                                                                      
CCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGAGGCGGTAATTAATAAGGCGCAGAGCGTCGTTTTGAACATAGGGTCT  >  NC_000913/4378653‑4378951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: