Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	44.3%	113.9 / 17.6	70	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (5/34);  new base G (31/0);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 5.36e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCC  >  NC_000913/354141‑354431                                                                                                                                                    |                                                                                                                                                 ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGa                                                                                                                                                <  2:328209/150‑1 (MQ=255) ggggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGa                                                                                                                                                <  2:1205402/150‑1 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgcg                                                                                                                                        <  2:1149888/151‑1 (MQ=255)        ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGTGGAAGATgc                                                                                                                                         >  2:204788/1‑150 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCt                                                                                                                                      <  1:142150/151‑1 (MQ=255)          gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCCGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGGGGGGGGGAAGAGgc                                                                                                                                         >  1:28559/1‑148 (MQ=255)            gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATgcgc                                                                                                                                       >  2:1279532/1‑148 (MQ=255)               gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGaaa                                                                                                                                  <  1:1242449/150‑1 (MQ=255)               gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                                 <  2:792531/151‑1 (MQ=255)               gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATgcg                                                                                                                                        >  1:435847/1‑144 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCTGAAAggg                                                                                                                               >  1:692101/1‑151 (MQ=255)                 tGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGGAATTAACGTTGCCGCGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGAATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGTCCTGacaagg                                                                                                                               >  2:177467/1‑146 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGGTGGAAggg                                                                                                                               >  2:299010/1‑149 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGCTGCGCTGAAAggg                                                                                                                               >  1:1181609/1‑149 (MQ=255)                   aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAggg                                                                                                                               >  2:287880/1‑149 (MQ=255)                    cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAg                                                                                                                            <  2:935508/151‑1 (MQ=255)                    cGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAggg                                                                                                                               >  2:747895/1‑148 (MQ=255)                     ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAgg                                                                                                                           >  2:657272/1‑151 (MQ=255)                      gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAggg                                                                                                                          >  1:517669/1‑150 (MQ=255)                         aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg‑tgg                                                                                                                       <  1:382686/150‑1 (MQ=255)                           cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg‑tggcg                                                                                                                     <  1:1341832/150‑1 (MQ=255)                           cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGCGGCGGGGGGTGGGGGGGAAAATGAGCTGGAAGGGGAgg‑gggggg                                                                                                                    >  1:457842/1‑151 (MQ=255)                            aCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAgg‑tggcggt                                                                                                                   <  1:718747/151‑1 (MQ=255)                                   tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGGGGTGAAAGGGGAGG‑GGGGGGTGGGGttt                                                                                                            >  1:772068/1‑151 (MQ=255)                                      cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGG‑GGGCGGGGGCGGTTGt                                                                                                          >  2:1149773/1‑150 (MQ=255)                                        ggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAAAAGCGCGGAAAGGGCAgg‑ggggggcgggggt                                                                                                             >  1:1042839/1‑141 (MQ=255)                                           gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTcc                                                                                                    <  2:473286/151‑1 (MQ=255)                                            cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCg                                                                                                   <  2:727888/151‑1 (MQ=255)                                            cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGGAAGGGGCGG‑GGGGGGGGGGGGTTGTCCTTCCg                                                                                                   >  1:1036213/1‑151 (MQ=255)                                               tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGGGGTGGAAGGGGAGG‑GGGGGGGGGGGGTTTTCATTCCgga                                                                                                 >  1:967531/1‑148 (MQ=255)                                                   gATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑AGGCGGTGGCGTTTGTCATTCCGAAagaga                                                                                            <  1:95083/151‑1 (MQ=255)                                                      gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGGAAGGGCAGG‑GGGCGGGGGGGTTTGTCATTTCGCAAGAGGGCg                                                                                         >  1:805052/1‑151 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                      <  2:765116/151‑1 (MQ=255)                                                         aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACa                                                                                      <  1:1335326/151‑1 (MQ=255)                                                            aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCTGGAAAGGGAGG‑TGGCGGTGGCGGTTGTCATTCCGAAAGAGAGGGACAGt                                                                                    >  1:817051/1‑150 (MQ=255)                                                                 tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCCCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGAAAAATGAGCCGAAAGGGCAGG‑GGGGGGGGGGGGTTGTTGTTTCGGAAAAgagagagcgattgg                                                                                >  1:559903/1‑141 (MQ=255)                                                                  gCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGaa                                                                              <  1:431953/150‑1 (MQ=255)                                                                   ccGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTCGGGGGGAAAAAAGCGCGGGAAGGGCAGGCCGGCGGTGG‑GTTTGTCCTTCCCGAAGAGAGCGAccgcgtgg                                                                                >  2:592644/1‑140 (MQ=255)                                                                         aTCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGGTGCGCTGAAAGGGGAGG‑GGGCGGGGGGGGTTGTTTTTTCGCAAGAGAGGGGCAGTCTGGAAGATCGt                                                                        >  2:130988/1‑149 (MQ=255)                                                                               tGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGG‑GGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTgg                                                                 >  2:866383/1‑150 (MQ=255)                                                                                ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCCGGAAGGGCAGG‑GGGGGGGGGGGGTTGTCATTCCGAAAAAGAGCGACAGTCTGGAAGATCGTggt                                                                     >  1:349436/1‑143 (MQ=255)                                                                                     cgcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTc                                                          <  1:8333/151‑1 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                       <  2:464181/151‑1 (MQ=255)                                                                                        gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGGAAAGGCAGG‑GGGCGGGGGCGGTTGTCATTACGAAAGAGAGGGACAGGCTGGGAGATCGGGGTGGGGCGCACTc                                                          >  1:296387/1‑148 (MQ=255)                                                                                          gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGG‑GGGCGGGGGCGGTTGGCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGc                                                        >  1:1229031/1‑148 (MQ=255)                                                                                            gaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCCGG‑GGGCGGGGGCGGTTGTCATTTCGAAAGAGAGCGACAGTTTGGAAGATCGGGATGTGGCGCACTccccag                                                     >  1:1158290/1‑144 (MQ=255)                                                                                                gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAAATGCGCCGAAAGGGCAGG‑TGGCGGTGGCGGTTGTCCTTCCGAAAAAGAGCGACAGTCTGGAAGATCGTGGTGTGGCGCACCCGCCAGAGAAgg                                               >  1:241323/1‑151 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                              <  2:399273/151‑1 (MQ=255)                                                                                                 aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGG‑GGGCGGGGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTGGAAGATCGGGATGTGGCGCCCTCGCAAGAGAAgg                                               >  2:1283037/1‑150 (MQ=255)                                                                                                  agagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGTGAAAGATGCGCCGAAAGGGGAGG‑GGGCGGGGGCGTTTGTCCTTTCGAAAGAGAGCGACAGTCTGGGAGATCGTGATGTTGCGCACTCGCAAGAGAAAGCg                                             >  1:1155879/1‑151 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCg                                             <  2:23809/150‑1 (MQ=255)                                                                                                   gagagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGa                                            <  1:680831/151‑1 (MQ=255)                                                                                                    agagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAt                                           <  1:808068/151‑1 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                          <  1:657179/151‑1 (MQ=255)                                                                                                     gagTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGAtt                                          <  1:833110/151‑1 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                       <  2:652278/150‑1 (MQ=255)                                                                                                         aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                       <  1:107104/150‑1 (MQ=255)                                                                                                             ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATgg                                      <  1:1095774/147‑1 (MQ=255)                                                                                                                gCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGG‑GGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgg                                >  2:611387/1‑150 (MQ=255)                                                                                                                 ccATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGGAAGGGCAGG‑TGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCtgggg                              >  1:612430/1‑149 (MQ=255)                                                                                                                       gggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGc                        <  2:461011/151‑1 (MQ=255)                                                                                                                             tGCCGATGTGGCGGCGGCTTGGGTGAAAAAAGCGCTTAAAGGGGCGG‑GGGCGGGGGGGTTTGTCCTTCCGAAAAAGAGCGACAGTCTGGGAGATCGTTGTGTTGCGCACTCGCAAGAGAAGGCGGATATGGCGGTGGGGGGCAGCCAGAtt                  >  1:458355/1‑151 (MQ=255)                                                                                                                              gCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAtt                  <  2:416267/150‑1 (MQ=255)                                                                                                                               ccGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                <  1:785288/151‑1 (MQ=255)                                                                                                                               ccGAAGTGGCGGTGGGTGGGGTGGAAGATGCGCTGAAAGGGCCGG‑GGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGAACGGGGTGTGGCGCCCTCGCAAGAGAAGGCGATTATGGCGCTGGTGGGCAGCCCGATTgg                >  1:317662/1‑151 (MQ=255)                                                                                                                                       gCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg        <  1:1126506/151‑1 (MQ=255)                                                                                                                                         ggtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgc      >  2:1116306/1‑151 (MQ=255)                                                                                                                                            ggtTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg    <  2:1061803/150‑1 (MQ=255)                                                                                                                                             gtTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc  <  2:1267732/151‑1 (MQ=255)                                                                                                                                             gtTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccgcc  <  1:542111/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGG‑TGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCC  >  NC_000913/354141‑354431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑